jacalin  (Vector Laboratories)


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    Vector Laboratories jacalin
    Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    jacalin  (Vector Laboratories)


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    Vector Laboratories jacalin
    Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    jacalin conjugated agarose beads  (Vector Laboratories)


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    Vector Laboratories jacalin conjugated agarose beads
    X-ray irradiation induces the expression of C1GalT1 and core 1 O-glycans in esophageal cancer cells. (A) C1GalT1 mRNA expression in esophageal cancer cells 48 h after irradiation was measured by qPCR. (B) C1GalT1 protein expression in esophageal cancer cells 48 h after irradiation was measured by western blot. (C) Expression of core 1 O-glycans was detected by flow cytometry. (D) Core 1 O-glycans were analyzed by <t>Jacalin</t> <t>lectin</t> blot. (E) Expression of core 1 O-glycans in esophageal cancer cells 48 h after irradiation was measured by flow cytometry. *P<0.05.
    Jacalin Conjugated Agarose Beads, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jacalin conjugated agarose beads/product/Vector Laboratories
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    1) Product Images from "Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation"

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation

    Journal: Journal of Cancer

    doi: 10.7150/jca.25252

    X-ray irradiation induces the expression of C1GalT1 and core 1 O-glycans in esophageal cancer cells. (A) C1GalT1 mRNA expression in esophageal cancer cells 48 h after irradiation was measured by qPCR. (B) C1GalT1 protein expression in esophageal cancer cells 48 h after irradiation was measured by western blot. (C) Expression of core 1 O-glycans was detected by flow cytometry. (D) Core 1 O-glycans were analyzed by Jacalin lectin blot. (E) Expression of core 1 O-glycans in esophageal cancer cells 48 h after irradiation was measured by flow cytometry. *P<0.05.
    Figure Legend Snippet: X-ray irradiation induces the expression of C1GalT1 and core 1 O-glycans in esophageal cancer cells. (A) C1GalT1 mRNA expression in esophageal cancer cells 48 h after irradiation was measured by qPCR. (B) C1GalT1 protein expression in esophageal cancer cells 48 h after irradiation was measured by western blot. (C) Expression of core 1 O-glycans was detected by flow cytometry. (D) Core 1 O-glycans were analyzed by Jacalin lectin blot. (E) Expression of core 1 O-glycans in esophageal cancer cells 48 h after irradiation was measured by flow cytometry. *P<0.05.

    Techniques Used: Irradiation, Expressing, Western Blot, Flow Cytometry

    C1GALT1 modifies O-glycans on β1-integrin. (A)Expression of core 1 O-glycans was detected by flow cytometry. (B) Core 1 O-glycans were analyzed by Jacalin lectin blot. (C) Cell lysates were pulled down (PD) by Jacalin and immunoblotted with an anti-β1-integrin antibody. (D) Cell lysates were immunoprecipitated with an anti-β1-integrin antibody and blotted with Jacalin or anti-β1-integrin antibody. **P<0.001.
    Figure Legend Snippet: C1GALT1 modifies O-glycans on β1-integrin. (A)Expression of core 1 O-glycans was detected by flow cytometry. (B) Core 1 O-glycans were analyzed by Jacalin lectin blot. (C) Cell lysates were pulled down (PD) by Jacalin and immunoblotted with an anti-β1-integrin antibody. (D) Cell lysates were immunoprecipitated with an anti-β1-integrin antibody and blotted with Jacalin or anti-β1-integrin antibody. **P<0.001.

    Techniques Used: Expressing, Flow Cytometry, Immunoprecipitation

    jacalin  (Vector Laboratories)


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    Vector Laboratories jacalin
    Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    jacalin  (Vector Laboratories)


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    Vector Laboratories jacalin
    Work flow illustrating the steps involved in the proteomic analysis of OA synovial fluid. OA synovial fluid samples were pooled and subjected to depletion of abundant proteins by MARS-6 LC column and lectin affinity chromatography using three <t>different</t> <t>lectins</t> (Concanavalin A, wheat germ agglutinin and <t>jacalin).</t> The depleted fraction was then subjected to SDS-PAGE, SCX and OFFGEL fractionation. The lectin enriched fraction was subjected to SDS-PAGE analysis and SCX fractionation. All fractions were analyzed on LTQ-Orbitrap Velos mass spectrometer. Sequest and Mascot algorithms were used to perform database searches. Subsequently, gene ontology-based functional characterization of the identified synovial fluid proteins was carried out. Further, validation by MRM- based assays was carried out for three proteins identified from discovery studies.
    Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Proteomic analysis of human osteoarthritis synovial fluid"

    Article Title: Proteomic analysis of human osteoarthritis synovial fluid

    Journal: Clinical proteomics

    doi: 10.1186/1559-0275-11-6

    Work flow illustrating the steps involved in the proteomic analysis of OA synovial fluid. OA synovial fluid samples were pooled and subjected to depletion of abundant proteins by MARS-6 LC column and lectin affinity chromatography using three different lectins (Concanavalin A, wheat germ agglutinin and jacalin). The depleted fraction was then subjected to SDS-PAGE, SCX and OFFGEL fractionation. The lectin enriched fraction was subjected to SDS-PAGE analysis and SCX fractionation. All fractions were analyzed on LTQ-Orbitrap Velos mass spectrometer. Sequest and Mascot algorithms were used to perform database searches. Subsequently, gene ontology-based functional characterization of the identified synovial fluid proteins was carried out. Further, validation by MRM- based assays was carried out for three proteins identified from discovery studies.
    Figure Legend Snippet: Work flow illustrating the steps involved in the proteomic analysis of OA synovial fluid. OA synovial fluid samples were pooled and subjected to depletion of abundant proteins by MARS-6 LC column and lectin affinity chromatography using three different lectins (Concanavalin A, wheat germ agglutinin and jacalin). The depleted fraction was then subjected to SDS-PAGE, SCX and OFFGEL fractionation. The lectin enriched fraction was subjected to SDS-PAGE analysis and SCX fractionation. All fractions were analyzed on LTQ-Orbitrap Velos mass spectrometer. Sequest and Mascot algorithms were used to perform database searches. Subsequently, gene ontology-based functional characterization of the identified synovial fluid proteins was carried out. Further, validation by MRM- based assays was carried out for three proteins identified from discovery studies.

    Techniques Used: Affinity Chromatography, SDS Page, Fractionation, Mass Spectrometry, Functional Assay

    jacalin  (Vector Laboratories)


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  • 93

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    Vector Laboratories jacalin
    Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    jacalin jac  (Vector Laboratories)


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    Vector Laboratories jacalin jac
    Lectins binding Galactose and N-acetyl-Galactosamine inhibit the interaction of GST-RadA 35-252 with type II mucin. Coated type II mucin (coated at 4 pmol per wells (40 nM)) was pre-incubated with various lectins: Wheat Germ Agglutinin (WGA, specific for GlcNAc), Ulex Europaeus Agglutinin (UEA, specific of Gal) and <t>Jacalin</t> <t>(JAC,</t> specific of Gal and GalNAc) (all at 100 µg/mL). A solid-phase binding assay of GST-RadA 35-252 was then conducted as explained in Materials and Methods. Results are expressed as means ± S.D. *: p < 0.01 ( n = 3).
    Jacalin Jac, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "RadA, a MSCRAMM Adhesin of the Dominant Symbiote Ruminococcus gnavus E1, Binds Human Immunoglobulins and Intestinal Mucins"

    Article Title: RadA, a MSCRAMM Adhesin of the Dominant Symbiote Ruminococcus gnavus E1, Binds Human Immunoglobulins and Intestinal Mucins

    Journal: Biomolecules

    doi: 10.3390/biom11111613

    Lectins binding Galactose and N-acetyl-Galactosamine inhibit the interaction of GST-RadA 35-252 with type II mucin. Coated type II mucin (coated at 4 pmol per wells (40 nM)) was pre-incubated with various lectins: Wheat Germ Agglutinin (WGA, specific for GlcNAc), Ulex Europaeus Agglutinin (UEA, specific of Gal) and Jacalin (JAC, specific of Gal and GalNAc) (all at 100 µg/mL). A solid-phase binding assay of GST-RadA 35-252 was then conducted as explained in Materials and Methods. Results are expressed as means ± S.D. *: p < 0.01 ( n = 3).
    Figure Legend Snippet: Lectins binding Galactose and N-acetyl-Galactosamine inhibit the interaction of GST-RadA 35-252 with type II mucin. Coated type II mucin (coated at 4 pmol per wells (40 nM)) was pre-incubated with various lectins: Wheat Germ Agglutinin (WGA, specific for GlcNAc), Ulex Europaeus Agglutinin (UEA, specific of Gal) and Jacalin (JAC, specific of Gal and GalNAc) (all at 100 µg/mL). A solid-phase binding assay of GST-RadA 35-252 was then conducted as explained in Materials and Methods. Results are expressed as means ± S.D. *: p < 0.01 ( n = 3).

    Techniques Used: Binding Assay, Incubation

    jacalin jac  (Vector Laboratories)


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    Vector Laboratories jacalin jac
    Lectins binding Galactose and N-acetyl-Galactosamine inhibit the interaction of GST-RadA 35-252 with type II mucin. Coated type II mucin (coated at 4 pmol per wells (40 nM)) was pre-incubated with various lectins: Wheat Germ Agglutinin (WGA, specific for GlcNAc), Ulex Europaeus Agglutinin (UEA, specific of Gal) and <t>Jacalin</t> <t>(JAC,</t> specific of Gal and GalNAc) (all at 100 µg/mL). A solid-phase binding assay of GST-RadA 35-252 was then conducted as explained in Materials and Methods. Results are expressed as means ± S.D. *: p < 0.01 ( n = 3).
    Jacalin Jac, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jacalin jac/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    1) Product Images from "RadA, a MSCRAMM Adhesin of the Dominant Symbiote Ruminococcus gnavus E1, Binds Human Immunoglobulins and Intestinal Mucins"

    Article Title: RadA, a MSCRAMM Adhesin of the Dominant Symbiote Ruminococcus gnavus E1, Binds Human Immunoglobulins and Intestinal Mucins

    Journal: Biomolecules

    doi: 10.3390/biom11111613

    Lectins binding Galactose and N-acetyl-Galactosamine inhibit the interaction of GST-RadA 35-252 with type II mucin. Coated type II mucin (coated at 4 pmol per wells (40 nM)) was pre-incubated with various lectins: Wheat Germ Agglutinin (WGA, specific for GlcNAc), Ulex Europaeus Agglutinin (UEA, specific of Gal) and Jacalin (JAC, specific of Gal and GalNAc) (all at 100 µg/mL). A solid-phase binding assay of GST-RadA 35-252 was then conducted as explained in Materials and Methods. Results are expressed as means ± S.D. *: p < 0.01 ( n = 3).
    Figure Legend Snippet: Lectins binding Galactose and N-acetyl-Galactosamine inhibit the interaction of GST-RadA 35-252 with type II mucin. Coated type II mucin (coated at 4 pmol per wells (40 nM)) was pre-incubated with various lectins: Wheat Germ Agglutinin (WGA, specific for GlcNAc), Ulex Europaeus Agglutinin (UEA, specific of Gal) and Jacalin (JAC, specific of Gal and GalNAc) (all at 100 µg/mL). A solid-phase binding assay of GST-RadA 35-252 was then conducted as explained in Materials and Methods. Results are expressed as means ± S.D. *: p < 0.01 ( n = 3).

    Techniques Used: Binding Assay, Incubation

    jacalin  (Vector Laboratories)


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    Vector Laboratories jacalin
    ( A ) The apical surface area of the epithelium expressing cilia in healthy (H) versus CF Airway (CF) Chips. Data are expressed as % total surface area per field. ( B ) Ciliary beat frequency (CBF) in Hz measured in healthy versus CF Airway Chips or Transwells (TWs). ( C ) Total mucin harvested from the epithelium after 7 days of culture under ALI from healthy versus CF Airway Chips, as measured by Alcian Blue staining. ( D ) Representative side view images of healthy and CF Airway Chips stained for MUC5AC with <t>jacalin</t> (JAC) or for MUC5B with wheat <t>germ</t> <t>agglutinin</t> (WGA) (right), and respective histograms below showing quantification of the area covered by the signal, and densitometric analysis (bar, 500 μm). All data represent mean ± SD obtained from at least 2 separate experiments; n = 22-30 ROIs from 2 different chips or TWs for A and B ; n = 6 biological replicates (3 chips from 2 healthy donors and 2 CF donors each) in C ; n = 4 chip replicates for D ; **** p<0.0001, *** p<0.001, ** p<0.01, * p <0.05.
    Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Modeling Pulmonary Cystic Fibrosis in a Human Lung Airway-on-a-chip"

    Article Title: Modeling Pulmonary Cystic Fibrosis in a Human Lung Airway-on-a-chip

    Journal: medRxiv

    doi: 10.1101/2021.07.15.21260407

    ( A ) The apical surface area of the epithelium expressing cilia in healthy (H) versus CF Airway (CF) Chips. Data are expressed as % total surface area per field. ( B ) Ciliary beat frequency (CBF) in Hz measured in healthy versus CF Airway Chips or Transwells (TWs). ( C ) Total mucin harvested from the epithelium after 7 days of culture under ALI from healthy versus CF Airway Chips, as measured by Alcian Blue staining. ( D ) Representative side view images of healthy and CF Airway Chips stained for MUC5AC with jacalin (JAC) or for MUC5B with wheat germ agglutinin (WGA) (right), and respective histograms below showing quantification of the area covered by the signal, and densitometric analysis (bar, 500 μm). All data represent mean ± SD obtained from at least 2 separate experiments; n = 22-30 ROIs from 2 different chips or TWs for A and B ; n = 6 biological replicates (3 chips from 2 healthy donors and 2 CF donors each) in C ; n = 4 chip replicates for D ; **** p<0.0001, *** p<0.001, ** p<0.01, * p <0.05.
    Figure Legend Snippet: ( A ) The apical surface area of the epithelium expressing cilia in healthy (H) versus CF Airway (CF) Chips. Data are expressed as % total surface area per field. ( B ) Ciliary beat frequency (CBF) in Hz measured in healthy versus CF Airway Chips or Transwells (TWs). ( C ) Total mucin harvested from the epithelium after 7 days of culture under ALI from healthy versus CF Airway Chips, as measured by Alcian Blue staining. ( D ) Representative side view images of healthy and CF Airway Chips stained for MUC5AC with jacalin (JAC) or for MUC5B with wheat germ agglutinin (WGA) (right), and respective histograms below showing quantification of the area covered by the signal, and densitometric analysis (bar, 500 μm). All data represent mean ± SD obtained from at least 2 separate experiments; n = 22-30 ROIs from 2 different chips or TWs for A and B ; n = 6 biological replicates (3 chips from 2 healthy donors and 2 CF donors each) in C ; n = 4 chip replicates for D ; **** p<0.0001, *** p<0.001, ** p<0.01, * p <0.05.

    Techniques Used: Expressing, Staining

    jacalin  (Vector Laboratories)


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    Vector Laboratories jacalin
    Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    unconjugated jacalin  (Vector Laboratories)


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    Vector Laboratories unconjugated jacalin
    KEY RESOURCES TABLE
    Unconjugated Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unconjugated jacalin/product/Vector Laboratories
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    1) Product Images from "Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies"

    Article Title: Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies

    Journal: Cell reports

    doi: 10.1016/j.celrep.2020.01.056

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Plasmid Preparation, Reporter Gene Assay, Knock-Out, Expressing, Software, Western Blot

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    Vector Laboratories jacalin
    Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories jacalin conjugated agarose beads
    X-ray irradiation induces the expression of C1GalT1 and core 1 O-glycans in esophageal cancer cells. (A) C1GalT1 mRNA expression in esophageal cancer cells 48 h after irradiation was measured by qPCR. (B) C1GalT1 protein expression in esophageal cancer cells 48 h after irradiation was measured by western blot. (C) Expression of core 1 O-glycans was detected by flow cytometry. (D) Core 1 O-glycans were analyzed by <t>Jacalin</t> <t>lectin</t> blot. (E) Expression of core 1 O-glycans in esophageal cancer cells 48 h after irradiation was measured by flow cytometry. *P<0.05.
    Jacalin Conjugated Agarose Beads, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories jacalin jac
    Lectins binding Galactose and N-acetyl-Galactosamine inhibit the interaction of GST-RadA 35-252 with type II mucin. Coated type II mucin (coated at 4 pmol per wells (40 nM)) was pre-incubated with various lectins: Wheat Germ Agglutinin (WGA, specific for GlcNAc), Ulex Europaeus Agglutinin (UEA, specific of Gal) and <t>Jacalin</t> <t>(JAC,</t> specific of Gal and GalNAc) (all at 100 µg/mL). A solid-phase binding assay of GST-RadA 35-252 was then conducted as explained in Materials and Methods. Results are expressed as means ± S.D. *: p < 0.01 ( n = 3).
    Jacalin Jac, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories unconjugated jacalin
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    Unconjugated Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    X-ray irradiation induces the expression of C1GalT1 and core 1 O-glycans in esophageal cancer cells. (A) C1GalT1 mRNA expression in esophageal cancer cells 48 h after irradiation was measured by qPCR. (B) C1GalT1 protein expression in esophageal cancer cells 48 h after irradiation was measured by western blot. (C) Expression of core 1 O-glycans was detected by flow cytometry. (D) Core 1 O-glycans were analyzed by Jacalin lectin blot. (E) Expression of core 1 O-glycans in esophageal cancer cells 48 h after irradiation was measured by flow cytometry. *P<0.05.

    Journal: Journal of Cancer

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation

    doi: 10.7150/jca.25252

    Figure Lengend Snippet: X-ray irradiation induces the expression of C1GalT1 and core 1 O-glycans in esophageal cancer cells. (A) C1GalT1 mRNA expression in esophageal cancer cells 48 h after irradiation was measured by qPCR. (B) C1GalT1 protein expression in esophageal cancer cells 48 h after irradiation was measured by western blot. (C) Expression of core 1 O-glycans was detected by flow cytometry. (D) Core 1 O-glycans were analyzed by Jacalin lectin blot. (E) Expression of core 1 O-glycans in esophageal cancer cells 48 h after irradiation was measured by flow cytometry. *P<0.05.

    Article Snippet: For lectin pull down assay, 1 mg total cell lysates were applied to Jacalin conjugated agarose beads (AL11153; Vector Laboratories) at 4°C overnight.

    Techniques: Irradiation, Expressing, Western Blot, Flow Cytometry

    C1GALT1 modifies O-glycans on β1-integrin. (A)Expression of core 1 O-glycans was detected by flow cytometry. (B) Core 1 O-glycans were analyzed by Jacalin lectin blot. (C) Cell lysates were pulled down (PD) by Jacalin and immunoblotted with an anti-β1-integrin antibody. (D) Cell lysates were immunoprecipitated with an anti-β1-integrin antibody and blotted with Jacalin or anti-β1-integrin antibody. **P<0.001.

    Journal: Journal of Cancer

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation

    doi: 10.7150/jca.25252

    Figure Lengend Snippet: C1GALT1 modifies O-glycans on β1-integrin. (A)Expression of core 1 O-glycans was detected by flow cytometry. (B) Core 1 O-glycans were analyzed by Jacalin lectin blot. (C) Cell lysates were pulled down (PD) by Jacalin and immunoblotted with an anti-β1-integrin antibody. (D) Cell lysates were immunoprecipitated with an anti-β1-integrin antibody and blotted with Jacalin or anti-β1-integrin antibody. **P<0.001.

    Article Snippet: For lectin pull down assay, 1 mg total cell lysates were applied to Jacalin conjugated agarose beads (AL11153; Vector Laboratories) at 4°C overnight.

    Techniques: Expressing, Flow Cytometry, Immunoprecipitation

    Lectins binding Galactose and N-acetyl-Galactosamine inhibit the interaction of GST-RadA 35-252 with type II mucin. Coated type II mucin (coated at 4 pmol per wells (40 nM)) was pre-incubated with various lectins: Wheat Germ Agglutinin (WGA, specific for GlcNAc), Ulex Europaeus Agglutinin (UEA, specific of Gal) and Jacalin (JAC, specific of Gal and GalNAc) (all at 100 µg/mL). A solid-phase binding assay of GST-RadA 35-252 was then conducted as explained in Materials and Methods. Results are expressed as means ± S.D. *: p < 0.01 ( n = 3).

    Journal: Biomolecules

    Article Title: RadA, a MSCRAMM Adhesin of the Dominant Symbiote Ruminococcus gnavus E1, Binds Human Immunoglobulins and Intestinal Mucins

    doi: 10.3390/biom11111613

    Figure Lengend Snippet: Lectins binding Galactose and N-acetyl-Galactosamine inhibit the interaction of GST-RadA 35-252 with type II mucin. Coated type II mucin (coated at 4 pmol per wells (40 nM)) was pre-incubated with various lectins: Wheat Germ Agglutinin (WGA, specific for GlcNAc), Ulex Europaeus Agglutinin (UEA, specific of Gal) and Jacalin (JAC, specific of Gal and GalNAc) (all at 100 µg/mL). A solid-phase binding assay of GST-RadA 35-252 was then conducted as explained in Materials and Methods. Results are expressed as means ± S.D. *: p < 0.01 ( n = 3).

    Article Snippet: The interaction of GST-RadA 35-252 (100 nM) with the human intestinal cells was also measured after 30 min pre-incubation of the cells at 37 °C with lectins (Wheat Germ Agglutinin (WGA), Ulex Europaeus Agglutinin (UEA) and Jacalin (JAC) (Vector Laboratories, Peterborough, UK) diluted at 100 µg/mL or after 30 min pre-incubation at 37 °C of GST-RadA 35-252 (100 nM) with 500 mM of Gal or GalNAc.

    Techniques: Binding Assay, Incubation

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Discovery of O-Linked Carbohydrate on HIV-1 Envelope and Its Role in Shielding against One Category of Broadly Neutralizing Antibodies

    doi: 10.1016/j.celrep.2020.01.056

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Unconjugated jacalin , Vector Laboratories , Cat# L-1150-10.

    Techniques: Recombinant, Plasmid Preparation, Reporter Gene Assay, Knock-Out, Expressing, Software, Western Blot