gsl i b 4 isolectin conjugated  (Vector Laboratories)


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    Vector Laboratories gsl i b 4 isolectin conjugated
    Gsl I B 4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gsl i b 4 isolectin conjugated  (Vector Laboratories)


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    Vector Laboratories gsl i b 4 isolectin conjugated
    Gsl I B 4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gsl 1 isolectin b4  (Vector Laboratories)


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    Vector Laboratories gsl 1 isolectin b4
    Gsl 1 Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    griffonia simplicifolia isolectin b4  (Vector Laboratories)


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    Vector Laboratories griffonia simplicifolia isolectin b4
    Analysis of migration, proliferation, and death of porcine ciliary epithelium (CE)-derived cells after subretinal transplantation. A : Quantification of the transplanted cells that had migrated into the neuroretina. CM-DiI-labeled cells were counted in 20 random sections from each eye. The middle third, containing the optic nerve, was considered to be the central retina and the two peripheral thirds, including the ora serrata, were considered to be the peripheral retina. The results are presented as the mean±SEM B , C : Cell proliferation assessed by Ki67 labeling in transplanted retinas. CM-DiI-positive cell aggregates in the subretinal space (red in B ) contained rare Ki67-labeled cells (green, arrow in C ), eight days after transplantation. D , E : Phagocytosis of transplanted cells by macrophages. CM-DiI-labeled particles (red in D , arrow) contained within <t>isolectin</t> <t>B4-positive</t> macrophages (green in E , arrow). The nuclei are labeled with 4',6-diamidino-2-phenylindole (DAPI; blue). Outer nuclear layer (ONL); inner nuclear layer (INL); and subretinal space (SS).
    Griffonia Simplicifolia Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "RPE and neuronal differentiation of allotransplantated porcine ciliary epithelium-derived cells"

    Article Title: RPE and neuronal differentiation of allotransplantated porcine ciliary epithelium-derived cells

    Journal: Molecular Vision

    doi:

    Analysis of migration, proliferation, and death of porcine ciliary epithelium (CE)-derived cells after subretinal transplantation. A : Quantification of the transplanted cells that had migrated into the neuroretina. CM-DiI-labeled cells were counted in 20 random sections from each eye. The middle third, containing the optic nerve, was considered to be the central retina and the two peripheral thirds, including the ora serrata, were considered to be the peripheral retina. The results are presented as the mean±SEM B , C : Cell proliferation assessed by Ki67 labeling in transplanted retinas. CM-DiI-positive cell aggregates in the subretinal space (red in B ) contained rare Ki67-labeled cells (green, arrow in C ), eight days after transplantation. D , E : Phagocytosis of transplanted cells by macrophages. CM-DiI-labeled particles (red in D , arrow) contained within isolectin B4-positive macrophages (green in E , arrow). The nuclei are labeled with 4',6-diamidino-2-phenylindole (DAPI; blue). Outer nuclear layer (ONL); inner nuclear layer (INL); and subretinal space (SS).
    Figure Legend Snippet: Analysis of migration, proliferation, and death of porcine ciliary epithelium (CE)-derived cells after subretinal transplantation. A : Quantification of the transplanted cells that had migrated into the neuroretina. CM-DiI-labeled cells were counted in 20 random sections from each eye. The middle third, containing the optic nerve, was considered to be the central retina and the two peripheral thirds, including the ora serrata, were considered to be the peripheral retina. The results are presented as the mean±SEM B , C : Cell proliferation assessed by Ki67 labeling in transplanted retinas. CM-DiI-positive cell aggregates in the subretinal space (red in B ) contained rare Ki67-labeled cells (green, arrow in C ), eight days after transplantation. D , E : Phagocytosis of transplanted cells by macrophages. CM-DiI-labeled particles (red in D , arrow) contained within isolectin B4-positive macrophages (green in E , arrow). The nuclei are labeled with 4',6-diamidino-2-phenylindole (DAPI; blue). Outer nuclear layer (ONL); inner nuclear layer (INL); and subretinal space (SS).

    Techniques Used: Migration, Derivative Assay, Transplantation Assay, Labeling

    gsl i isolectin b4  (Vector Laboratories)


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    Vector Laboratories gsl i isolectin b4
    Changes in the mRNA and protein expression of P2X 7 and P2X 4 receptors in the striatum and substantia nigra obtained from P2X 7 receptor wild-type (WT) and P2X 7 -/- (KO) mice after in vivo MPTP treatment . Mice (6-8 mice per group) were injected i.p. with 4 × 20 mg/kg of MPTP or saline (SAL). A-C. After decapitation, the brains were removed immediately, total RNA extracted from the striatum (A, C) and substantia nigra (B) and then reverse-transcribed to cDNA. Data are displayed as the means ± S.E.M. Asterisks indicate significant differences from the corresponding saline-injected mice or between genotypes as indicated (* P < 0.05, ***P < 0.0001). D, E. and F. Immunofluorescent staining for P2X 7 receptor and microglial cells in striatal sections of saline- and MPTP-treated WT and P2X 7 -/- mice. Merged pictures. Fluorescein-labeled <t>GSL</t> <t>I</t> - <t>isolectin</t> <t>B4</t> is a marker for endothelial (see stars in picture
    Gsl I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Lack of neuroprotection in the absence of P2X7 receptors in toxin-induced animal models of Parkinson's disease"

    Article Title: Lack of neuroprotection in the absence of P2X7 receptors in toxin-induced animal models of Parkinson's disease

    Journal: Molecular Neurodegeneration

    doi: 10.1186/1750-1326-6-28

    Changes in the mRNA and protein expression of P2X 7 and P2X 4 receptors in the striatum and substantia nigra obtained from P2X 7 receptor wild-type (WT) and P2X 7 -/- (KO) mice after in vivo MPTP treatment . Mice (6-8 mice per group) were injected i.p. with 4 × 20 mg/kg of MPTP or saline (SAL). A-C. After decapitation, the brains were removed immediately, total RNA extracted from the striatum (A, C) and substantia nigra (B) and then reverse-transcribed to cDNA. Data are displayed as the means ± S.E.M. Asterisks indicate significant differences from the corresponding saline-injected mice or between genotypes as indicated (* P < 0.05, ***P < 0.0001). D, E. and F. Immunofluorescent staining for P2X 7 receptor and microglial cells in striatal sections of saline- and MPTP-treated WT and P2X 7 -/- mice. Merged pictures. Fluorescein-labeled GSL I - isolectin B4 is a marker for endothelial (see stars in picture
    Figure Legend Snippet: Changes in the mRNA and protein expression of P2X 7 and P2X 4 receptors in the striatum and substantia nigra obtained from P2X 7 receptor wild-type (WT) and P2X 7 -/- (KO) mice after in vivo MPTP treatment . Mice (6-8 mice per group) were injected i.p. with 4 × 20 mg/kg of MPTP or saline (SAL). A-C. After decapitation, the brains were removed immediately, total RNA extracted from the striatum (A, C) and substantia nigra (B) and then reverse-transcribed to cDNA. Data are displayed as the means ± S.E.M. Asterisks indicate significant differences from the corresponding saline-injected mice or between genotypes as indicated (* P < 0.05, ***P < 0.0001). D, E. and F. Immunofluorescent staining for P2X 7 receptor and microglial cells in striatal sections of saline- and MPTP-treated WT and P2X 7 -/- mice. Merged pictures. Fluorescein-labeled GSL I - isolectin B4 is a marker for endothelial (see stars in picture "F") and microglial cells. P2X 7 receptors were labeled with a P2X 7 -DyLight 549 conjugate (red). Orange means the same localization for both stains. DAPI (in Vectashield mounting medium) labels nuclei (blue). Scale bar: 20 μm. D. Detail of the striatal section of saline-treated WT mouse brain. Inserts (arrows) show the localization of P2X 7 receptors in FITC-labeled microglial cells. E. Only microglial cells are labeled (green, insert, arrow) in the striatal section of MPTP-treated WT mouse brain. F. Microglia (arrow, insert) and endothelial cells (stars) are labeled by the isolectin, but no P2X 7 immunofluorescence is visible in the striatal sections of saline-treated P2X 7 -/- mouse brain.

    Techniques Used: Expressing, In Vivo, Injection, Staining, Labeling, Marker, Immunofluorescence

    biotin conjugated griffonia simplicifolia lectin gsl i isolectin b 4  (Vector Laboratories)


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    Vector Laboratories biotin conjugated griffonia simplicifolia lectin gsl i isolectin b 4
    Biotin Conjugated Griffonia Simplicifolia Lectin Gsl I Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    isolectin b4  (Vector Laboratories)


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    Vector Laboratories isolectin b4
    Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fitc conjugated griffonia simplicifolia lectin  (Vector Laboratories)


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    Vector Laboratories fitc conjugated griffonia simplicifolia lectin
    Fitc Conjugated Griffonia Simplicifolia Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated griffonia simplicifolia lectin/product/Vector Laboratories
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    bandeiraea simplicifolia isolectin b4  (Vector Laboratories)


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    Vector Laboratories bandeiraea simplicifolia isolectin b4
    The effect of AKE and CA on retinal neovascularization in OIR mice . (a) The retinal neovascular tufts were visualized using <t>isolectin</t> <t>B4</t> staining. (b) Quantification results are expressed as neovascular tufts on the retina surface. The bar graph values represent the mean ± SEM, n = 7, ∗ p < 0.05 versus OIR mice.
    Bandeiraea Simplicifolia Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Aster koraiensis Extract and Chlorogenic Acid Inhibit Retinal Angiogenesis in a Mouse Model of Oxygen-Induced Retinopathy"

    Article Title: Aster koraiensis Extract and Chlorogenic Acid Inhibit Retinal Angiogenesis in a Mouse Model of Oxygen-Induced Retinopathy

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2018/6402650

    The effect of AKE and CA on retinal neovascularization in OIR mice . (a) The retinal neovascular tufts were visualized using isolectin B4 staining. (b) Quantification results are expressed as neovascular tufts on the retina surface. The bar graph values represent the mean ± SEM, n = 7, ∗ p < 0.05 versus OIR mice.
    Figure Legend Snippet: The effect of AKE and CA on retinal neovascularization in OIR mice . (a) The retinal neovascular tufts were visualized using isolectin B4 staining. (b) Quantification results are expressed as neovascular tufts on the retina surface. The bar graph values represent the mean ± SEM, n = 7, ∗ p < 0.05 versus OIR mice.

    Techniques Used: Staining

    fitc conjugated griffonia simplicifolia lectin i isolectinb4  (Vector Laboratories)


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    Vector Laboratories fitc conjugated griffonia simplicifolia lectin i isolectinb4
    Different vascular morphologies displayed a similar pattern of gene expression. High variability of vascular morphology was observed in tumors of large volume. Large lumen-containing vessels ((b, d) vessel lumen size > 25 μ m) existed next to much smaller blood vessels that did not contain a visible lumen (a, c). (a, b) Immunohistochemical staining for CD31: pictures represent different regions within the same large tumor (Magnification 100x). (c, d) Cryosections from large tumors were stained with <t>GSL-I</t> <t>IsolectinB4:FITC</t> and the different vascular morphologies were separately isolated by laser microdissection along the depicted lines, magnification 400x. Microdissection of these different vascular phenotypes followed by gene expression profiling did not reveal a differential pattern of mRNA expression of angiogenesis- and vascular stability-regulating genes (e), nor of the Notch family of genes that has recently been identified to be important regulators of both angiogenic sprouting and endothelial-pericyte adhesion (f), but demonstrated that the lumen-containing vessels were associated with significantly higher levels of α SMA mRNA compared to the small vessels without lumen (g). (e)–(g) Mean values + SD of duplicate qPCR measurements of three large tumors. ND: not detectable. * P < 0.05.
    Fitc Conjugated Griffonia Simplicifolia Lectin I Isolectinb4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated griffonia simplicifolia lectin i isolectinb4/product/Vector Laboratories
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    1) Product Images from "Tumor Vascular Morphology Undergoes Dramatic Changes during Outgrowth of B16 Melanoma While Proangiogenic Gene Expression Remains Unchanged"

    Article Title: Tumor Vascular Morphology Undergoes Dramatic Changes during Outgrowth of B16 Melanoma While Proangiogenic Gene Expression Remains Unchanged

    Journal: ISRN Oncology

    doi: 10.5402/2011/409308

    Different vascular morphologies displayed a similar pattern of gene expression. High variability of vascular morphology was observed in tumors of large volume. Large lumen-containing vessels ((b, d) vessel lumen size > 25 μ m) existed next to much smaller blood vessels that did not contain a visible lumen (a, c). (a, b) Immunohistochemical staining for CD31: pictures represent different regions within the same large tumor (Magnification 100x). (c, d) Cryosections from large tumors were stained with GSL-I IsolectinB4:FITC and the different vascular morphologies were separately isolated by laser microdissection along the depicted lines, magnification 400x. Microdissection of these different vascular phenotypes followed by gene expression profiling did not reveal a differential pattern of mRNA expression of angiogenesis- and vascular stability-regulating genes (e), nor of the Notch family of genes that has recently been identified to be important regulators of both angiogenic sprouting and endothelial-pericyte adhesion (f), but demonstrated that the lumen-containing vessels were associated with significantly higher levels of α SMA mRNA compared to the small vessels without lumen (g). (e)–(g) Mean values + SD of duplicate qPCR measurements of three large tumors. ND: not detectable. * P < 0.05.
    Figure Legend Snippet: Different vascular morphologies displayed a similar pattern of gene expression. High variability of vascular morphology was observed in tumors of large volume. Large lumen-containing vessels ((b, d) vessel lumen size > 25 μ m) existed next to much smaller blood vessels that did not contain a visible lumen (a, c). (a, b) Immunohistochemical staining for CD31: pictures represent different regions within the same large tumor (Magnification 100x). (c, d) Cryosections from large tumors were stained with GSL-I IsolectinB4:FITC and the different vascular morphologies were separately isolated by laser microdissection along the depicted lines, magnification 400x. Microdissection of these different vascular phenotypes followed by gene expression profiling did not reveal a differential pattern of mRNA expression of angiogenesis- and vascular stability-regulating genes (e), nor of the Notch family of genes that has recently been identified to be important regulators of both angiogenic sprouting and endothelial-pericyte adhesion (f), but demonstrated that the lumen-containing vessels were associated with significantly higher levels of α SMA mRNA compared to the small vessels without lumen (g). (e)–(g) Mean values + SD of duplicate qPCR measurements of three large tumors. ND: not detectable. * P < 0.05.

    Techniques Used: Expressing, Immunohistochemical staining, Staining, Isolation, Laser Capture Microdissection

    biotin conjugated griffonia simplicifolia lectin i isolectin b4  (Vector Laboratories)


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    Vector Laboratories biotin conjugated griffonia simplicifolia lectin i isolectin b4
    Reduced extracellular collagen deposition ( A–C; picrosirius red = red), increased capillary density ( D–F; <t>Isolectin</t> <t>B4</t> = red), and increased proliferation ( G–I; Ki67 = red; nuclei = blue; cTnT = green) were observed in the border areas at day 28 after BMC transplantation (BMC group), compared to the PBS control (CON group). These effects were all abolished by anti-HMGB1 antibody neutralization (AB group), but not by control IgG administration (IgG group). Representative images of only BMC and AB groups are present (see for additional images). Scale bars = 50 µm in A, B, G, H and 30 µm in D, E . *: p <0.05 versus the CON group, † : p <0.05 versus the BMC group, ‡ : p <0.05 versus the IgG group, mean±SEM for n = 5∼7 in each group.
    Biotin Conjugated Griffonia Simplicifolia Lectin I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated griffonia simplicifolia lectin i isolectin b4/product/Vector Laboratories
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    1) Product Images from "Extracellular High Mobility Group Box 1 Plays a Role in the Effect of Bone Marrow Mononuclear Cell Transplantation for Heart Failure"

    Article Title: Extracellular High Mobility Group Box 1 Plays a Role in the Effect of Bone Marrow Mononuclear Cell Transplantation for Heart Failure

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0076908

    Reduced extracellular collagen deposition ( A–C; picrosirius red = red), increased capillary density ( D–F; Isolectin B4 = red), and increased proliferation ( G–I; Ki67 = red; nuclei = blue; cTnT = green) were observed in the border areas at day 28 after BMC transplantation (BMC group), compared to the PBS control (CON group). These effects were all abolished by anti-HMGB1 antibody neutralization (AB group), but not by control IgG administration (IgG group). Representative images of only BMC and AB groups are present (see for additional images). Scale bars = 50 µm in A, B, G, H and 30 µm in D, E . *: p <0.05 versus the CON group, † : p <0.05 versus the BMC group, ‡ : p <0.05 versus the IgG group, mean±SEM for n = 5∼7 in each group.
    Figure Legend Snippet: Reduced extracellular collagen deposition ( A–C; picrosirius red = red), increased capillary density ( D–F; Isolectin B4 = red), and increased proliferation ( G–I; Ki67 = red; nuclei = blue; cTnT = green) were observed in the border areas at day 28 after BMC transplantation (BMC group), compared to the PBS control (CON group). These effects were all abolished by anti-HMGB1 antibody neutralization (AB group), but not by control IgG administration (IgG group). Representative images of only BMC and AB groups are present (see for additional images). Scale bars = 50 µm in A, B, G, H and 30 µm in D, E . *: p <0.05 versus the CON group, † : p <0.05 versus the BMC group, ‡ : p <0.05 versus the IgG group, mean±SEM for n = 5∼7 in each group.

    Techniques Used: Transplantation Assay, Neutralization

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    Vector Laboratories gsl i b 4 isolectin conjugated
    Gsl I B 4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories griffonia simplicifolia isolectin b4
    Analysis of migration, proliferation, and death of porcine ciliary epithelium (CE)-derived cells after subretinal transplantation. A : Quantification of the transplanted cells that had migrated into the neuroretina. CM-DiI-labeled cells were counted in 20 random sections from each eye. The middle third, containing the optic nerve, was considered to be the central retina and the two peripheral thirds, including the ora serrata, were considered to be the peripheral retina. The results are presented as the mean±SEM B , C : Cell proliferation assessed by Ki67 labeling in transplanted retinas. CM-DiI-positive cell aggregates in the subretinal space (red in B ) contained rare Ki67-labeled cells (green, arrow in C ), eight days after transplantation. D , E : Phagocytosis of transplanted cells by macrophages. CM-DiI-labeled particles (red in D , arrow) contained within <t>isolectin</t> <t>B4-positive</t> macrophages (green in E , arrow). The nuclei are labeled with 4',6-diamidino-2-phenylindole (DAPI; blue). Outer nuclear layer (ONL); inner nuclear layer (INL); and subretinal space (SS).
    Griffonia Simplicifolia Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/griffonia simplicifolia isolectin b4/product/Vector Laboratories
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    Changes in the mRNA and protein expression of P2X 7 and P2X 4 receptors in the striatum and substantia nigra obtained from P2X 7 receptor wild-type (WT) and P2X 7 -/- (KO) mice after in vivo MPTP treatment . Mice (6-8 mice per group) were injected i.p. with 4 × 20 mg/kg of MPTP or saline (SAL). A-C. After decapitation, the brains were removed immediately, total RNA extracted from the striatum (A, C) and substantia nigra (B) and then reverse-transcribed to cDNA. Data are displayed as the means ± S.E.M. Asterisks indicate significant differences from the corresponding saline-injected mice or between genotypes as indicated (* P < 0.05, ***P < 0.0001). D, E. and F. Immunofluorescent staining for P2X 7 receptor and microglial cells in striatal sections of saline- and MPTP-treated WT and P2X 7 -/- mice. Merged pictures. Fluorescein-labeled <t>GSL</t> <t>I</t> - <t>isolectin</t> <t>B4</t> is a marker for endothelial (see stars in picture
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    Changes in the mRNA and protein expression of P2X 7 and P2X 4 receptors in the striatum and substantia nigra obtained from P2X 7 receptor wild-type (WT) and P2X 7 -/- (KO) mice after in vivo MPTP treatment . Mice (6-8 mice per group) were injected i.p. with 4 × 20 mg/kg of MPTP or saline (SAL). A-C. After decapitation, the brains were removed immediately, total RNA extracted from the striatum (A, C) and substantia nigra (B) and then reverse-transcribed to cDNA. Data are displayed as the means ± S.E.M. Asterisks indicate significant differences from the corresponding saline-injected mice or between genotypes as indicated (* P < 0.05, ***P < 0.0001). D, E. and F. Immunofluorescent staining for P2X 7 receptor and microglial cells in striatal sections of saline- and MPTP-treated WT and P2X 7 -/- mice. Merged pictures. Fluorescein-labeled <t>GSL</t> <t>I</t> - <t>isolectin</t> <t>B4</t> is a marker for endothelial (see stars in picture
    Biotin Conjugated Griffonia Simplicifolia Lectin Gsl I Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Changes in the mRNA and protein expression of P2X 7 and P2X 4 receptors in the striatum and substantia nigra obtained from P2X 7 receptor wild-type (WT) and P2X 7 -/- (KO) mice after in vivo MPTP treatment . Mice (6-8 mice per group) were injected i.p. with 4 × 20 mg/kg of MPTP or saline (SAL). A-C. After decapitation, the brains were removed immediately, total RNA extracted from the striatum (A, C) and substantia nigra (B) and then reverse-transcribed to cDNA. Data are displayed as the means ± S.E.M. Asterisks indicate significant differences from the corresponding saline-injected mice or between genotypes as indicated (* P < 0.05, ***P < 0.0001). D, E. and F. Immunofluorescent staining for P2X 7 receptor and microglial cells in striatal sections of saline- and MPTP-treated WT and P2X 7 -/- mice. Merged pictures. Fluorescein-labeled <t>GSL</t> <t>I</t> - <t>isolectin</t> <t>B4</t> is a marker for endothelial (see stars in picture
    Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Changes in the mRNA and protein expression of P2X 7 and P2X 4 receptors in the striatum and substantia nigra obtained from P2X 7 receptor wild-type (WT) and P2X 7 -/- (KO) mice after in vivo MPTP treatment . Mice (6-8 mice per group) were injected i.p. with 4 × 20 mg/kg of MPTP or saline (SAL). A-C. After decapitation, the brains were removed immediately, total RNA extracted from the striatum (A, C) and substantia nigra (B) and then reverse-transcribed to cDNA. Data are displayed as the means ± S.E.M. Asterisks indicate significant differences from the corresponding saline-injected mice or between genotypes as indicated (* P < 0.05, ***P < 0.0001). D, E. and F. Immunofluorescent staining for P2X 7 receptor and microglial cells in striatal sections of saline- and MPTP-treated WT and P2X 7 -/- mice. Merged pictures. Fluorescein-labeled <t>GSL</t> <t>I</t> - <t>isolectin</t> <t>B4</t> is a marker for endothelial (see stars in picture
    Fitc Conjugated Griffonia Simplicifolia Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The effect of AKE and CA on retinal neovascularization in OIR mice . (a) The retinal neovascular tufts were visualized using <t>isolectin</t> <t>B4</t> staining. (b) Quantification results are expressed as neovascular tufts on the retina surface. The bar graph values represent the mean ± SEM, n = 7, ∗ p < 0.05 versus OIR mice.
    Bandeiraea Simplicifolia Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Different vascular morphologies displayed a similar pattern of gene expression. High variability of vascular morphology was observed in tumors of large volume. Large lumen-containing vessels ((b, d) vessel lumen size > 25 μ m) existed next to much smaller blood vessels that did not contain a visible lumen (a, c). (a, b) Immunohistochemical staining for CD31: pictures represent different regions within the same large tumor (Magnification 100x). (c, d) Cryosections from large tumors were stained with <t>GSL-I</t> <t>IsolectinB4:FITC</t> and the different vascular morphologies were separately isolated by laser microdissection along the depicted lines, magnification 400x. Microdissection of these different vascular phenotypes followed by gene expression profiling did not reveal a differential pattern of mRNA expression of angiogenesis- and vascular stability-regulating genes (e), nor of the Notch family of genes that has recently been identified to be important regulators of both angiogenic sprouting and endothelial-pericyte adhesion (f), but demonstrated that the lumen-containing vessels were associated with significantly higher levels of α SMA mRNA compared to the small vessels without lumen (g). (e)–(g) Mean values + SD of duplicate qPCR measurements of three large tumors. ND: not detectable. * P < 0.05.
    Fitc Conjugated Griffonia Simplicifolia Lectin I Isolectinb4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Reduced extracellular collagen deposition ( A–C; picrosirius red = red), increased capillary density ( D–F; <t>Isolectin</t> <t>B4</t> = red), and increased proliferation ( G–I; Ki67 = red; nuclei = blue; cTnT = green) were observed in the border areas at day 28 after BMC transplantation (BMC group), compared to the PBS control (CON group). These effects were all abolished by anti-HMGB1 antibody neutralization (AB group), but not by control IgG administration (IgG group). Representative images of only BMC and AB groups are present (see for additional images). Scale bars = 50 µm in A, B, G, H and 30 µm in D, E . *: p <0.05 versus the CON group, † : p <0.05 versus the BMC group, ‡ : p <0.05 versus the IgG group, mean±SEM for n = 5∼7 in each group.
    Biotin Conjugated Griffonia Simplicifolia Lectin I Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Analysis of migration, proliferation, and death of porcine ciliary epithelium (CE)-derived cells after subretinal transplantation. A : Quantification of the transplanted cells that had migrated into the neuroretina. CM-DiI-labeled cells were counted in 20 random sections from each eye. The middle third, containing the optic nerve, was considered to be the central retina and the two peripheral thirds, including the ora serrata, were considered to be the peripheral retina. The results are presented as the mean±SEM B , C : Cell proliferation assessed by Ki67 labeling in transplanted retinas. CM-DiI-positive cell aggregates in the subretinal space (red in B ) contained rare Ki67-labeled cells (green, arrow in C ), eight days after transplantation. D , E : Phagocytosis of transplanted cells by macrophages. CM-DiI-labeled particles (red in D , arrow) contained within isolectin B4-positive macrophages (green in E , arrow). The nuclei are labeled with 4',6-diamidino-2-phenylindole (DAPI; blue). Outer nuclear layer (ONL); inner nuclear layer (INL); and subretinal space (SS).

    Journal: Molecular Vision

    Article Title: RPE and neuronal differentiation of allotransplantated porcine ciliary epithelium-derived cells

    doi:

    Figure Lengend Snippet: Analysis of migration, proliferation, and death of porcine ciliary epithelium (CE)-derived cells after subretinal transplantation. A : Quantification of the transplanted cells that had migrated into the neuroretina. CM-DiI-labeled cells were counted in 20 random sections from each eye. The middle third, containing the optic nerve, was considered to be the central retina and the two peripheral thirds, including the ora serrata, were considered to be the peripheral retina. The results are presented as the mean±SEM B , C : Cell proliferation assessed by Ki67 labeling in transplanted retinas. CM-DiI-positive cell aggregates in the subretinal space (red in B ) contained rare Ki67-labeled cells (green, arrow in C ), eight days after transplantation. D , E : Phagocytosis of transplanted cells by macrophages. CM-DiI-labeled particles (red in D , arrow) contained within isolectin B4-positive macrophages (green in E , arrow). The nuclei are labeled with 4',6-diamidino-2-phenylindole (DAPI; blue). Outer nuclear layer (ONL); inner nuclear layer (INL); and subretinal space (SS).

    Article Snippet: For isolectin B4 staining, sections were blocked in 5% BSA for 30 min, incubated with biothynilated Griffonia simplicifolia Isolectin B4 (Vector) 1:100 for 1 h, washed for 3×5 min with PBS, and were finally incubated with streptavidin-FITC 1:200 for 1 h. For the immunocytochemistry of the differentiated cells, post-fixation glass slides were washed 3× in PBS, incubated in 10% NGS, 0.3% Triton X-100, and 0.01% NaN 3 in PBS for 1 h at room temperature, followed by overnight incubation at 4 °C.

    Techniques: Migration, Derivative Assay, Transplantation Assay, Labeling

    Changes in the mRNA and protein expression of P2X 7 and P2X 4 receptors in the striatum and substantia nigra obtained from P2X 7 receptor wild-type (WT) and P2X 7 -/- (KO) mice after in vivo MPTP treatment . Mice (6-8 mice per group) were injected i.p. with 4 × 20 mg/kg of MPTP or saline (SAL). A-C. After decapitation, the brains were removed immediately, total RNA extracted from the striatum (A, C) and substantia nigra (B) and then reverse-transcribed to cDNA. Data are displayed as the means ± S.E.M. Asterisks indicate significant differences from the corresponding saline-injected mice or between genotypes as indicated (* P < 0.05, ***P < 0.0001). D, E. and F. Immunofluorescent staining for P2X 7 receptor and microglial cells in striatal sections of saline- and MPTP-treated WT and P2X 7 -/- mice. Merged pictures. Fluorescein-labeled GSL I - isolectin B4 is a marker for endothelial (see stars in picture

    Journal: Molecular Neurodegeneration

    Article Title: Lack of neuroprotection in the absence of P2X7 receptors in toxin-induced animal models of Parkinson's disease

    doi: 10.1186/1750-1326-6-28

    Figure Lengend Snippet: Changes in the mRNA and protein expression of P2X 7 and P2X 4 receptors in the striatum and substantia nigra obtained from P2X 7 receptor wild-type (WT) and P2X 7 -/- (KO) mice after in vivo MPTP treatment . Mice (6-8 mice per group) were injected i.p. with 4 × 20 mg/kg of MPTP or saline (SAL). A-C. After decapitation, the brains were removed immediately, total RNA extracted from the striatum (A, C) and substantia nigra (B) and then reverse-transcribed to cDNA. Data are displayed as the means ± S.E.M. Asterisks indicate significant differences from the corresponding saline-injected mice or between genotypes as indicated (* P < 0.05, ***P < 0.0001). D, E. and F. Immunofluorescent staining for P2X 7 receptor and microglial cells in striatal sections of saline- and MPTP-treated WT and P2X 7 -/- mice. Merged pictures. Fluorescein-labeled GSL I - isolectin B4 is a marker for endothelial (see stars in picture "F") and microglial cells. P2X 7 receptors were labeled with a P2X 7 -DyLight 549 conjugate (red). Orange means the same localization for both stains. DAPI (in Vectashield mounting medium) labels nuclei (blue). Scale bar: 20 μm. D. Detail of the striatal section of saline-treated WT mouse brain. Inserts (arrows) show the localization of P2X 7 receptors in FITC-labeled microglial cells. E. Only microglial cells are labeled (green, insert, arrow) in the striatal section of MPTP-treated WT mouse brain. F. Microglia (arrow, insert) and endothelial cells (stars) are labeled by the isolectin, but no P2X 7 immunofluorescence is visible in the striatal sections of saline-treated P2X 7 -/- mouse brain.

    Article Snippet: After thorough washing, fluorescein-labeled GSL I - isolectin B4 (Vector Laboratories; Burlingame, CA, USA) in a 1:100 dilution and 1:400 DyLight 549 AffiniPure Donkey Anti-Goat IgG (Jackson ImmunoResearch) were applied overnight.

    Techniques: Expressing, In Vivo, Injection, Staining, Labeling, Marker, Immunofluorescence

    The effect of AKE and CA on retinal neovascularization in OIR mice . (a) The retinal neovascular tufts were visualized using isolectin B4 staining. (b) Quantification results are expressed as neovascular tufts on the retina surface. The bar graph values represent the mean ± SEM, n = 7, ∗ p < 0.05 versus OIR mice.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Aster koraiensis Extract and Chlorogenic Acid Inhibit Retinal Angiogenesis in a Mouse Model of Oxygen-Induced Retinopathy

    doi: 10.1155/2018/6402650

    Figure Lengend Snippet: The effect of AKE and CA on retinal neovascularization in OIR mice . (a) The retinal neovascular tufts were visualized using isolectin B4 staining. (b) Quantification results are expressed as neovascular tufts on the retina surface. The bar graph values represent the mean ± SEM, n = 7, ∗ p < 0.05 versus OIR mice.

    Article Snippet: The neovascular tufts in the retina were stained with rhodamine-labeled Bandeiraea simplicifolia isolectin B4 (Vector Laboratories Ltd., Burlingame, CA, USA).

    Techniques: Staining

    Different vascular morphologies displayed a similar pattern of gene expression. High variability of vascular morphology was observed in tumors of large volume. Large lumen-containing vessels ((b, d) vessel lumen size > 25 μ m) existed next to much smaller blood vessels that did not contain a visible lumen (a, c). (a, b) Immunohistochemical staining for CD31: pictures represent different regions within the same large tumor (Magnification 100x). (c, d) Cryosections from large tumors were stained with GSL-I IsolectinB4:FITC and the different vascular morphologies were separately isolated by laser microdissection along the depicted lines, magnification 400x. Microdissection of these different vascular phenotypes followed by gene expression profiling did not reveal a differential pattern of mRNA expression of angiogenesis- and vascular stability-regulating genes (e), nor of the Notch family of genes that has recently been identified to be important regulators of both angiogenic sprouting and endothelial-pericyte adhesion (f), but demonstrated that the lumen-containing vessels were associated with significantly higher levels of α SMA mRNA compared to the small vessels without lumen (g). (e)–(g) Mean values + SD of duplicate qPCR measurements of three large tumors. ND: not detectable. * P < 0.05.

    Journal: ISRN Oncology

    Article Title: Tumor Vascular Morphology Undergoes Dramatic Changes during Outgrowth of B16 Melanoma While Proangiogenic Gene Expression Remains Unchanged

    doi: 10.5402/2011/409308

    Figure Lengend Snippet: Different vascular morphologies displayed a similar pattern of gene expression. High variability of vascular morphology was observed in tumors of large volume. Large lumen-containing vessels ((b, d) vessel lumen size > 25 μ m) existed next to much smaller blood vessels that did not contain a visible lumen (a, c). (a, b) Immunohistochemical staining for CD31: pictures represent different regions within the same large tumor (Magnification 100x). (c, d) Cryosections from large tumors were stained with GSL-I IsolectinB4:FITC and the different vascular morphologies were separately isolated by laser microdissection along the depicted lines, magnification 400x. Microdissection of these different vascular phenotypes followed by gene expression profiling did not reveal a differential pattern of mRNA expression of angiogenesis- and vascular stability-regulating genes (e), nor of the Notch family of genes that has recently been identified to be important regulators of both angiogenic sprouting and endothelial-pericyte adhesion (f), but demonstrated that the lumen-containing vessels were associated with significantly higher levels of α SMA mRNA compared to the small vessels without lumen (g). (e)–(g) Mean values + SD of duplicate qPCR measurements of three large tumors. ND: not detectable. * P < 0.05.

    Article Snippet: Tumor vascular segments representing small vascular profiles and large lumen-containing vessels were separately microdissected using an LMD6000 Laser Microdissection system (Leica, Wetzlar, Germany) from 9 μ m cryosections of large tumors mounted on polyethylene-terephthalate membranes on steel frames (Leica) that were stained with FITC-conjugated Griffonia Simplicifolia Lectin I IsolectinB4 (GSLI-isolectin B4: Vector Labs, Burlingame, CA, USA).

    Techniques: Expressing, Immunohistochemical staining, Staining, Isolation, Laser Capture Microdissection

    Reduced extracellular collagen deposition ( A–C; picrosirius red = red), increased capillary density ( D–F; Isolectin B4 = red), and increased proliferation ( G–I; Ki67 = red; nuclei = blue; cTnT = green) were observed in the border areas at day 28 after BMC transplantation (BMC group), compared to the PBS control (CON group). These effects were all abolished by anti-HMGB1 antibody neutralization (AB group), but not by control IgG administration (IgG group). Representative images of only BMC and AB groups are present (see for additional images). Scale bars = 50 µm in A, B, G, H and 30 µm in D, E . *: p <0.05 versus the CON group, † : p <0.05 versus the BMC group, ‡ : p <0.05 versus the IgG group, mean±SEM for n = 5∼7 in each group.

    Journal: PLoS ONE

    Article Title: Extracellular High Mobility Group Box 1 Plays a Role in the Effect of Bone Marrow Mononuclear Cell Transplantation for Heart Failure

    doi: 10.1371/journal.pone.0076908

    Figure Lengend Snippet: Reduced extracellular collagen deposition ( A–C; picrosirius red = red), increased capillary density ( D–F; Isolectin B4 = red), and increased proliferation ( G–I; Ki67 = red; nuclei = blue; cTnT = green) were observed in the border areas at day 28 after BMC transplantation (BMC group), compared to the PBS control (CON group). These effects were all abolished by anti-HMGB1 antibody neutralization (AB group), but not by control IgG administration (IgG group). Representative images of only BMC and AB groups are present (see for additional images). Scale bars = 50 µm in A, B, G, H and 30 µm in D, E . *: p <0.05 versus the CON group, † : p <0.05 versus the BMC group, ‡ : p <0.05 versus the IgG group, mean±SEM for n = 5∼7 in each group.

    Article Snippet: Cryosections were cut and incubated with biotin conjugated Griffonia simplicifolia lectin I-isolectin B4 (1∶100, Vector), monoclonal anti-rat CD68 antibody (1∶100, AbD Serotec), monoclonal anti-rat CD86 antibody (1∶50, BD), monoclonal anti-rat CD163 antibody (1∶100, AbD Serotec), monoclonal anti-rat Ki-67 antibody (1∶50, DakoCytomation), and/or polyclonal anti-rat cardiac troponin-T (cTnT) antibody (1∶200, HyTest) followed by visualization using appropriate fluorophore-conjugated secondary antibodies (Molecular Probes).

    Techniques: Transplantation Assay, Neutralization