wheat germ agglutinin wga  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Laboratories wheat germ agglutinin wga
    The 1D gel electrophoresis and Coomassie protein staining of potentially enriched glycoproteins from human tear sample pools. The image shows the eluate fractions of single-lectin <t>columns</t> <t>(ConA,</t> JAC, and <t>WGA)</t> and the multi-lectin column device (combination of ConA, JAC, and WGA, termed 3L). An untreated tear sample pool (50 µg) was included in this experiment to match potentially enriched glycoproteins with the native tear film proteome. The most abundant protein markers were labeled in the respective mass range.
    Wheat Germ Agglutinin Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheat germ agglutinin wga/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin wga - by Bioz Stars, 2024-05
    96/100 stars

    Images

    1) Product Images from "Lectin-Based Affinity Enrichment and Characterization of N-Glycoproteins from Human Tear Film by Mass Spectrometry"

    Article Title: Lectin-Based Affinity Enrichment and Characterization of N-Glycoproteins from Human Tear Film by Mass Spectrometry

    Journal: Molecules

    doi: 10.3390/molecules28020648

    The 1D gel electrophoresis and Coomassie protein staining of potentially enriched glycoproteins from human tear sample pools. The image shows the eluate fractions of single-lectin columns (ConA, JAC, and WGA) and the multi-lectin column device (combination of ConA, JAC, and WGA, termed 3L). An untreated tear sample pool (50 µg) was included in this experiment to match potentially enriched glycoproteins with the native tear film proteome. The most abundant protein markers were labeled in the respective mass range.
    Figure Legend Snippet: The 1D gel electrophoresis and Coomassie protein staining of potentially enriched glycoproteins from human tear sample pools. The image shows the eluate fractions of single-lectin columns (ConA, JAC, and WGA) and the multi-lectin column device (combination of ConA, JAC, and WGA, termed 3L). An untreated tear sample pool (50 µg) was included in this experiment to match potentially enriched glycoproteins with the native tear film proteome. The most abundant protein markers were labeled in the respective mass range.

    Techniques Used: Nucleic Acid Electrophoresis, Staining, Labeling

    The 1D gel electrophoresis and glycoprotein/Coomassie staining of enriched glycoproteins from human tear sample pools. Both images illustrate the eluate as well as the flow-through (FT) fractions of the 3L multi-lectin column (combination of ConA, JAC, and WGA) and the 4L multi-lectin column system (combination of ConA, JAC, WGA, and UEA I). Prior to affinity enrichment, the tear sample pools were partially pre-treated with PNGase F to release the N-glycans from the glycoproteins. An untreated tear sample pool (50 µg) was also included to match the enriched glycoprotein spots with the native tear film proteome. In addition, a glycosylated protein standard (horseradish peroxidase, +CTRL), as well as a non-glycosylated protein standard (soybean trypsin inhibitor, -CTRL), were either included as a positive or negative control in this experiment. ( A ) Glycoprotein staining of the 1D gel which specifically colored the glycan motifs of the tear film glycoproteins/protein standards as magenta stained protein bands. ( B ) Coomassie protein staining of the 1D gel to visualize all the proteins as brilliant blue protein bands. The most abundant protein markers were labeled in the respective mass range.
    Figure Legend Snippet: The 1D gel electrophoresis and glycoprotein/Coomassie staining of enriched glycoproteins from human tear sample pools. Both images illustrate the eluate as well as the flow-through (FT) fractions of the 3L multi-lectin column (combination of ConA, JAC, and WGA) and the 4L multi-lectin column system (combination of ConA, JAC, WGA, and UEA I). Prior to affinity enrichment, the tear sample pools were partially pre-treated with PNGase F to release the N-glycans from the glycoproteins. An untreated tear sample pool (50 µg) was also included to match the enriched glycoprotein spots with the native tear film proteome. In addition, a glycosylated protein standard (horseradish peroxidase, +CTRL), as well as a non-glycosylated protein standard (soybean trypsin inhibitor, -CTRL), were either included as a positive or negative control in this experiment. ( A ) Glycoprotein staining of the 1D gel which specifically colored the glycan motifs of the tear film glycoproteins/protein standards as magenta stained protein bands. ( B ) Coomassie protein staining of the 1D gel to visualize all the proteins as brilliant blue protein bands. The most abundant protein markers were labeled in the respective mass range.

    Techniques Used: Nucleic Acid Electrophoresis, Staining, Negative Control, Labeling

    List of  lectins  and their respective glycan specificity.
    Figure Legend Snippet: List of lectins and their respective glycan specificity.

    Techniques Used:

    wheat germ agglutinin wga  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Laboratories wheat germ agglutinin wga
    The 1D gel electrophoresis and Coomassie protein staining of potentially enriched glycoproteins from human tear sample pools. The image shows the eluate fractions of single-lectin <t>columns</t> <t>(ConA,</t> JAC, and <t>WGA)</t> and the multi-lectin column device (combination of ConA, JAC, and WGA, termed 3L). An untreated tear sample pool (50 µg) was included in this experiment to match potentially enriched glycoproteins with the native tear film proteome. The most abundant protein markers were labeled in the respective mass range.
    Wheat Germ Agglutinin Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheat germ agglutinin wga/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin wga - by Bioz Stars, 2024-05
    96/100 stars

    Images

    1) Product Images from "Lectin-Based Affinity Enrichment and Characterization of N-Glycoproteins from Human Tear Film by Mass Spectrometry"

    Article Title: Lectin-Based Affinity Enrichment and Characterization of N-Glycoproteins from Human Tear Film by Mass Spectrometry

    Journal: Molecules

    doi: 10.3390/molecules28020648

    The 1D gel electrophoresis and Coomassie protein staining of potentially enriched glycoproteins from human tear sample pools. The image shows the eluate fractions of single-lectin columns (ConA, JAC, and WGA) and the multi-lectin column device (combination of ConA, JAC, and WGA, termed 3L). An untreated tear sample pool (50 µg) was included in this experiment to match potentially enriched glycoproteins with the native tear film proteome. The most abundant protein markers were labeled in the respective mass range.
    Figure Legend Snippet: The 1D gel electrophoresis and Coomassie protein staining of potentially enriched glycoproteins from human tear sample pools. The image shows the eluate fractions of single-lectin columns (ConA, JAC, and WGA) and the multi-lectin column device (combination of ConA, JAC, and WGA, termed 3L). An untreated tear sample pool (50 µg) was included in this experiment to match potentially enriched glycoproteins with the native tear film proteome. The most abundant protein markers were labeled in the respective mass range.

    Techniques Used: Nucleic Acid Electrophoresis, Staining, Labeling

    The 1D gel electrophoresis and glycoprotein/Coomassie staining of enriched glycoproteins from human tear sample pools. Both images illustrate the eluate as well as the flow-through (FT) fractions of the 3L multi-lectin column (combination of ConA, JAC, and WGA) and the 4L multi-lectin column system (combination of ConA, JAC, WGA, and UEA I). Prior to affinity enrichment, the tear sample pools were partially pre-treated with PNGase F to release the N-glycans from the glycoproteins. An untreated tear sample pool (50 µg) was also included to match the enriched glycoprotein spots with the native tear film proteome. In addition, a glycosylated protein standard (horseradish peroxidase, +CTRL), as well as a non-glycosylated protein standard (soybean trypsin inhibitor, -CTRL), were either included as a positive or negative control in this experiment. ( A ) Glycoprotein staining of the 1D gel which specifically colored the glycan motifs of the tear film glycoproteins/protein standards as magenta stained protein bands. ( B ) Coomassie protein staining of the 1D gel to visualize all the proteins as brilliant blue protein bands. The most abundant protein markers were labeled in the respective mass range.
    Figure Legend Snippet: The 1D gel electrophoresis and glycoprotein/Coomassie staining of enriched glycoproteins from human tear sample pools. Both images illustrate the eluate as well as the flow-through (FT) fractions of the 3L multi-lectin column (combination of ConA, JAC, and WGA) and the 4L multi-lectin column system (combination of ConA, JAC, WGA, and UEA I). Prior to affinity enrichment, the tear sample pools were partially pre-treated with PNGase F to release the N-glycans from the glycoproteins. An untreated tear sample pool (50 µg) was also included to match the enriched glycoprotein spots with the native tear film proteome. In addition, a glycosylated protein standard (horseradish peroxidase, +CTRL), as well as a non-glycosylated protein standard (soybean trypsin inhibitor, -CTRL), were either included as a positive or negative control in this experiment. ( A ) Glycoprotein staining of the 1D gel which specifically colored the glycan motifs of the tear film glycoproteins/protein standards as magenta stained protein bands. ( B ) Coomassie protein staining of the 1D gel to visualize all the proteins as brilliant blue protein bands. The most abundant protein markers were labeled in the respective mass range.

    Techniques Used: Nucleic Acid Electrophoresis, Staining, Negative Control, Labeling

    List of  lectins  and their respective glycan specificity.
    Figure Legend Snippet: List of lectins and their respective glycan specificity.

    Techniques Used:

    wheat germ agglutinin  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Laboratories wheat germ agglutinin
    Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheat germ agglutinin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin - by Bioz Stars, 2024-05
    96/100 stars

    Images

    wheat germ agglutinin  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Laboratories wheat germ agglutinin
    Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheat germ agglutinin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin - by Bioz Stars, 2024-05
    96/100 stars

    Images

    wga rhodamine  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Laboratories wga rhodamine
    (A) Expression of Meox2 mRNA in the most lateral region (rounded brackets) of presomitic mesoderm at stage 14 (dorsal view and transverse section), 16 (dorsal view), 17/18 (dorsal view and transverse section) and 23 (transverse section). Dotted lines indicate the position of the medial and lateral population of myogenic cells. Vertical lines define the limit between anterior and trunk region. Nc, notochord. (B) Lineage tracing experiments: embryos were injected <t>with</t> <t>WGA-rhodamine</t> (red) in the most lateral presomitic mesoderm (a) or co-injected with WGA-fluorescein (green) in the lateral presomitic mesoderm (c) at stage 13. Transverse sections of the embryo at the trunk level and at stages 18 or 23; embryo injected with WGA-rhodamine and submitted to indirect immunofluorescence with 12/101 antibody followed by secondary Alexa fluor 488 anti-mouse antibody (green) (b) or injected with the both tracers (d, e and f). WGA-rhodamine fluorescence (d), WGA-fluorescein fluorescence (e), merge (f). Dotted lines indicate the bilateral symmetry plan. In (a) and (c): Vertical lines define the limit between anterior and trunk region. (a) and (c) dorsal views, anterior side on the left. (C, D and E). Ablation experiments of presomitic mesoderm at stage 14. After microdissection experiments, embryos were fixed at stage 19 or at the tailbud stage to analyze meox2 and pax3 expression respectively. (C) sham-operated embryos, ectoderm was incised at the lateral level (line), ectoderm and mesoderm were separated from each other on the lateral side, but mesoderm was not removed. (D) Same operation on the mediolateral level but superficial mesoderm was removed (hatched zone) (E) Same operation on the lateral level but superficial mesoderm was removed (hatched zone). Brackets show the axis level corresponding to incision. For complete statistical data, see supporting information, .
    Wga Rhodamine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wga rhodamine/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wga rhodamine - by Bioz Stars, 2024-05
    96/100 stars

    Images

    1) Product Images from "Mef2d Acts Upstream of Muscle Identity Genes and Couples Lateral Myogenesis to Dermomyotome Formation in Xenopus laevis"

    Article Title: Mef2d Acts Upstream of Muscle Identity Genes and Couples Lateral Myogenesis to Dermomyotome Formation in Xenopus laevis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0052359

    (A) Expression of Meox2 mRNA in the most lateral region (rounded brackets) of presomitic mesoderm at stage 14 (dorsal view and transverse section), 16 (dorsal view), 17/18 (dorsal view and transverse section) and 23 (transverse section). Dotted lines indicate the position of the medial and lateral population of myogenic cells. Vertical lines define the limit between anterior and trunk region. Nc, notochord. (B) Lineage tracing experiments: embryos were injected with WGA-rhodamine (red) in the most lateral presomitic mesoderm (a) or co-injected with WGA-fluorescein (green) in the lateral presomitic mesoderm (c) at stage 13. Transverse sections of the embryo at the trunk level and at stages 18 or 23; embryo injected with WGA-rhodamine and submitted to indirect immunofluorescence with 12/101 antibody followed by secondary Alexa fluor 488 anti-mouse antibody (green) (b) or injected with the both tracers (d, e and f). WGA-rhodamine fluorescence (d), WGA-fluorescein fluorescence (e), merge (f). Dotted lines indicate the bilateral symmetry plan. In (a) and (c): Vertical lines define the limit between anterior and trunk region. (a) and (c) dorsal views, anterior side on the left. (C, D and E). Ablation experiments of presomitic mesoderm at stage 14. After microdissection experiments, embryos were fixed at stage 19 or at the tailbud stage to analyze meox2 and pax3 expression respectively. (C) sham-operated embryos, ectoderm was incised at the lateral level (line), ectoderm and mesoderm were separated from each other on the lateral side, but mesoderm was not removed. (D) Same operation on the mediolateral level but superficial mesoderm was removed (hatched zone) (E) Same operation on the lateral level but superficial mesoderm was removed (hatched zone). Brackets show the axis level corresponding to incision. For complete statistical data, see supporting information, .
    Figure Legend Snippet: (A) Expression of Meox2 mRNA in the most lateral region (rounded brackets) of presomitic mesoderm at stage 14 (dorsal view and transverse section), 16 (dorsal view), 17/18 (dorsal view and transverse section) and 23 (transverse section). Dotted lines indicate the position of the medial and lateral population of myogenic cells. Vertical lines define the limit between anterior and trunk region. Nc, notochord. (B) Lineage tracing experiments: embryos were injected with WGA-rhodamine (red) in the most lateral presomitic mesoderm (a) or co-injected with WGA-fluorescein (green) in the lateral presomitic mesoderm (c) at stage 13. Transverse sections of the embryo at the trunk level and at stages 18 or 23; embryo injected with WGA-rhodamine and submitted to indirect immunofluorescence with 12/101 antibody followed by secondary Alexa fluor 488 anti-mouse antibody (green) (b) or injected with the both tracers (d, e and f). WGA-rhodamine fluorescence (d), WGA-fluorescein fluorescence (e), merge (f). Dotted lines indicate the bilateral symmetry plan. In (a) and (c): Vertical lines define the limit between anterior and trunk region. (a) and (c) dorsal views, anterior side on the left. (C, D and E). Ablation experiments of presomitic mesoderm at stage 14. After microdissection experiments, embryos were fixed at stage 19 or at the tailbud stage to analyze meox2 and pax3 expression respectively. (C) sham-operated embryos, ectoderm was incised at the lateral level (line), ectoderm and mesoderm were separated from each other on the lateral side, but mesoderm was not removed. (D) Same operation on the mediolateral level but superficial mesoderm was removed (hatched zone) (E) Same operation on the lateral level but superficial mesoderm was removed (hatched zone). Brackets show the axis level corresponding to incision. For complete statistical data, see supporting information, .

    Techniques Used: Expressing, Injection, Immunofluorescence, Fluorescence, Laser Capture Microdissection

    wheat germ agglutinin wga  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Laboratories wheat germ agglutinin wga
    A–C : A. Shank 1A B. <t>WGA</t> labeling at the OPL. Arrowheads indicate WGA rod spherule labeling, and arrows indicate the location of WGA labeling of cone pedicles. C. YFP cone bipolar cell dendrites. D–F : Shank 1A (blue) and WGA (red) co-localize at cone terminals in the OPL. G–I: YFP cone bipolar cell dendrites (yellow) synapse with WGA (red) labeled cone terminals, which cradle Shank 1A immunoreactive puncta (blue). Scale bar is 10 µm.
    Wheat Germ Agglutinin Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheat germ agglutinin wga/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin wga - by Bioz Stars, 2024-05
    96/100 stars

    Images

    1) Product Images from "Association of Shank 1A Scaffolding Protein with Cone Photoreceptor Terminals in the Mammalian Retina"

    Article Title: Association of Shank 1A Scaffolding Protein with Cone Photoreceptor Terminals in the Mammalian Retina

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0043463

    A–C : A. Shank 1A B. WGA labeling at the OPL. Arrowheads indicate WGA rod spherule labeling, and arrows indicate the location of WGA labeling of cone pedicles. C. YFP cone bipolar cell dendrites. D–F : Shank 1A (blue) and WGA (red) co-localize at cone terminals in the OPL. G–I: YFP cone bipolar cell dendrites (yellow) synapse with WGA (red) labeled cone terminals, which cradle Shank 1A immunoreactive puncta (blue). Scale bar is 10 µm.
    Figure Legend Snippet: A–C : A. Shank 1A B. WGA labeling at the OPL. Arrowheads indicate WGA rod spherule labeling, and arrows indicate the location of WGA labeling of cone pedicles. C. YFP cone bipolar cell dendrites. D–F : Shank 1A (blue) and WGA (red) co-localize at cone terminals in the OPL. G–I: YFP cone bipolar cell dendrites (yellow) synapse with WGA (red) labeled cone terminals, which cradle Shank 1A immunoreactive puncta (blue). Scale bar is 10 µm.

    Techniques Used: Labeling

    Antibodies and Lectins.
    Figure Legend Snippet: Antibodies and Lectins.

    Techniques Used: Sequencing, Generated, Binding Assay, Recombinant, Transduction, Plasmid Preparation

    wheat germ agglutinin  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Laboratories wheat germ agglutinin
    Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheat germ agglutinin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin - by Bioz Stars, 2024-05
    96/100 stars

    Images

    unconjugated wheat germ agglutinin  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Laboratories unconjugated wheat germ agglutinin
    Unconjugated Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unconjugated wheat germ agglutinin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    unconjugated wheat germ agglutinin - by Bioz Stars, 2024-05
    96/100 stars

    Images

    wheat germ agglutinin  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Laboratories wheat germ agglutinin
    Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheat germ agglutinin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin - by Bioz Stars, 2024-05
    96/100 stars

    Images

    wheat germ agglutinin  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Laboratories wheat germ agglutinin
    Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheat germ agglutinin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin - by Bioz Stars, 2024-05
    96/100 stars

    Images

    rhodamine conjugated wheat germ agglutinin  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Vector Laboratories rhodamine conjugated wheat germ agglutinin
    Rhodamine Conjugated Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rhodamine conjugated wheat germ agglutinin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rhodamine conjugated wheat germ agglutinin - by Bioz Stars, 2024-05
    96/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Vector Laboratories wheat germ agglutinin wga
    The 1D gel electrophoresis and Coomassie protein staining of potentially enriched glycoproteins from human tear sample pools. The image shows the eluate fractions of single-lectin <t>columns</t> <t>(ConA,</t> JAC, and <t>WGA)</t> and the multi-lectin column device (combination of ConA, JAC, and WGA, termed 3L). An untreated tear sample pool (50 µg) was included in this experiment to match potentially enriched glycoproteins with the native tear film proteome. The most abundant protein markers were labeled in the respective mass range.
    Wheat Germ Agglutinin Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheat germ agglutinin wga/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin wga - by Bioz Stars, 2024-05
    96/100 stars
      Buy from Supplier

    96
    Vector Laboratories wheat germ agglutinin
    The 1D gel electrophoresis and Coomassie protein staining of potentially enriched glycoproteins from human tear sample pools. The image shows the eluate fractions of single-lectin <t>columns</t> <t>(ConA,</t> JAC, and <t>WGA)</t> and the multi-lectin column device (combination of ConA, JAC, and WGA, termed 3L). An untreated tear sample pool (50 µg) was included in this experiment to match potentially enriched glycoproteins with the native tear film proteome. The most abundant protein markers were labeled in the respective mass range.
    Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wheat germ agglutinin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wheat germ agglutinin - by Bioz Stars, 2024-05
    96/100 stars
      Buy from Supplier

    96
    Vector Laboratories wga rhodamine
    (A) Expression of Meox2 mRNA in the most lateral region (rounded brackets) of presomitic mesoderm at stage 14 (dorsal view and transverse section), 16 (dorsal view), 17/18 (dorsal view and transverse section) and 23 (transverse section). Dotted lines indicate the position of the medial and lateral population of myogenic cells. Vertical lines define the limit between anterior and trunk region. Nc, notochord. (B) Lineage tracing experiments: embryos were injected <t>with</t> <t>WGA-rhodamine</t> (red) in the most lateral presomitic mesoderm (a) or co-injected with WGA-fluorescein (green) in the lateral presomitic mesoderm (c) at stage 13. Transverse sections of the embryo at the trunk level and at stages 18 or 23; embryo injected with WGA-rhodamine and submitted to indirect immunofluorescence with 12/101 antibody followed by secondary Alexa fluor 488 anti-mouse antibody (green) (b) or injected with the both tracers (d, e and f). WGA-rhodamine fluorescence (d), WGA-fluorescein fluorescence (e), merge (f). Dotted lines indicate the bilateral symmetry plan. In (a) and (c): Vertical lines define the limit between anterior and trunk region. (a) and (c) dorsal views, anterior side on the left. (C, D and E). Ablation experiments of presomitic mesoderm at stage 14. After microdissection experiments, embryos were fixed at stage 19 or at the tailbud stage to analyze meox2 and pax3 expression respectively. (C) sham-operated embryos, ectoderm was incised at the lateral level (line), ectoderm and mesoderm were separated from each other on the lateral side, but mesoderm was not removed. (D) Same operation on the mediolateral level but superficial mesoderm was removed (hatched zone) (E) Same operation on the lateral level but superficial mesoderm was removed (hatched zone). Brackets show the axis level corresponding to incision. For complete statistical data, see supporting information, .
    Wga Rhodamine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wga rhodamine/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wga rhodamine - by Bioz Stars, 2024-05
    96/100 stars
      Buy from Supplier

    96
    Vector Laboratories unconjugated wheat germ agglutinin
    (A) Expression of Meox2 mRNA in the most lateral region (rounded brackets) of presomitic mesoderm at stage 14 (dorsal view and transverse section), 16 (dorsal view), 17/18 (dorsal view and transverse section) and 23 (transverse section). Dotted lines indicate the position of the medial and lateral population of myogenic cells. Vertical lines define the limit between anterior and trunk region. Nc, notochord. (B) Lineage tracing experiments: embryos were injected <t>with</t> <t>WGA-rhodamine</t> (red) in the most lateral presomitic mesoderm (a) or co-injected with WGA-fluorescein (green) in the lateral presomitic mesoderm (c) at stage 13. Transverse sections of the embryo at the trunk level and at stages 18 or 23; embryo injected with WGA-rhodamine and submitted to indirect immunofluorescence with 12/101 antibody followed by secondary Alexa fluor 488 anti-mouse antibody (green) (b) or injected with the both tracers (d, e and f). WGA-rhodamine fluorescence (d), WGA-fluorescein fluorescence (e), merge (f). Dotted lines indicate the bilateral symmetry plan. In (a) and (c): Vertical lines define the limit between anterior and trunk region. (a) and (c) dorsal views, anterior side on the left. (C, D and E). Ablation experiments of presomitic mesoderm at stage 14. After microdissection experiments, embryos were fixed at stage 19 or at the tailbud stage to analyze meox2 and pax3 expression respectively. (C) sham-operated embryos, ectoderm was incised at the lateral level (line), ectoderm and mesoderm were separated from each other on the lateral side, but mesoderm was not removed. (D) Same operation on the mediolateral level but superficial mesoderm was removed (hatched zone) (E) Same operation on the lateral level but superficial mesoderm was removed (hatched zone). Brackets show the axis level corresponding to incision. For complete statistical data, see supporting information, .
    Unconjugated Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/unconjugated wheat germ agglutinin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    unconjugated wheat germ agglutinin - by Bioz Stars, 2024-05
    96/100 stars
      Buy from Supplier

    96
    Vector Laboratories rhodamine conjugated wheat germ agglutinin
    (A) Expression of Meox2 mRNA in the most lateral region (rounded brackets) of presomitic mesoderm at stage 14 (dorsal view and transverse section), 16 (dorsal view), 17/18 (dorsal view and transverse section) and 23 (transverse section). Dotted lines indicate the position of the medial and lateral population of myogenic cells. Vertical lines define the limit between anterior and trunk region. Nc, notochord. (B) Lineage tracing experiments: embryos were injected <t>with</t> <t>WGA-rhodamine</t> (red) in the most lateral presomitic mesoderm (a) or co-injected with WGA-fluorescein (green) in the lateral presomitic mesoderm (c) at stage 13. Transverse sections of the embryo at the trunk level and at stages 18 or 23; embryo injected with WGA-rhodamine and submitted to indirect immunofluorescence with 12/101 antibody followed by secondary Alexa fluor 488 anti-mouse antibody (green) (b) or injected with the both tracers (d, e and f). WGA-rhodamine fluorescence (d), WGA-fluorescein fluorescence (e), merge (f). Dotted lines indicate the bilateral symmetry plan. In (a) and (c): Vertical lines define the limit between anterior and trunk region. (a) and (c) dorsal views, anterior side on the left. (C, D and E). Ablation experiments of presomitic mesoderm at stage 14. After microdissection experiments, embryos were fixed at stage 19 or at the tailbud stage to analyze meox2 and pax3 expression respectively. (C) sham-operated embryos, ectoderm was incised at the lateral level (line), ectoderm and mesoderm were separated from each other on the lateral side, but mesoderm was not removed. (D) Same operation on the mediolateral level but superficial mesoderm was removed (hatched zone) (E) Same operation on the lateral level but superficial mesoderm was removed (hatched zone). Brackets show the axis level corresponding to incision. For complete statistical data, see supporting information, .
    Rhodamine Conjugated Wheat Germ Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rhodamine conjugated wheat germ agglutinin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rhodamine conjugated wheat germ agglutinin - by Bioz Stars, 2024-05
    96/100 stars
      Buy from Supplier

    Image Search Results


    The 1D gel electrophoresis and Coomassie protein staining of potentially enriched glycoproteins from human tear sample pools. The image shows the eluate fractions of single-lectin columns (ConA, JAC, and WGA) and the multi-lectin column device (combination of ConA, JAC, and WGA, termed 3L). An untreated tear sample pool (50 µg) was included in this experiment to match potentially enriched glycoproteins with the native tear film proteome. The most abundant protein markers were labeled in the respective mass range.

    Journal: Molecules

    Article Title: Lectin-Based Affinity Enrichment and Characterization of N-Glycoproteins from Human Tear Film by Mass Spectrometry

    doi: 10.3390/molecules28020648

    Figure Lengend Snippet: The 1D gel electrophoresis and Coomassie protein staining of potentially enriched glycoproteins from human tear sample pools. The image shows the eluate fractions of single-lectin columns (ConA, JAC, and WGA) and the multi-lectin column device (combination of ConA, JAC, and WGA, termed 3L). An untreated tear sample pool (50 µg) was included in this experiment to match potentially enriched glycoproteins with the native tear film proteome. The most abundant protein markers were labeled in the respective mass range.

    Article Snippet: For the glycoprotein/glycopeptide enrichment of the pooled tear film samples (see method ), we used the agarose-bound lectins concanavalin (ConA), wheat germ agglutinin (WGA), jacalin (JAC), and ulex europaeus agglutinin I (UEA I) from the manufacturer Vector Laboratories (Burlingame, CA, USA).

    Techniques: Nucleic Acid Electrophoresis, Staining, Labeling

    The 1D gel electrophoresis and glycoprotein/Coomassie staining of enriched glycoproteins from human tear sample pools. Both images illustrate the eluate as well as the flow-through (FT) fractions of the 3L multi-lectin column (combination of ConA, JAC, and WGA) and the 4L multi-lectin column system (combination of ConA, JAC, WGA, and UEA I). Prior to affinity enrichment, the tear sample pools were partially pre-treated with PNGase F to release the N-glycans from the glycoproteins. An untreated tear sample pool (50 µg) was also included to match the enriched glycoprotein spots with the native tear film proteome. In addition, a glycosylated protein standard (horseradish peroxidase, +CTRL), as well as a non-glycosylated protein standard (soybean trypsin inhibitor, -CTRL), were either included as a positive or negative control in this experiment. ( A ) Glycoprotein staining of the 1D gel which specifically colored the glycan motifs of the tear film glycoproteins/protein standards as magenta stained protein bands. ( B ) Coomassie protein staining of the 1D gel to visualize all the proteins as brilliant blue protein bands. The most abundant protein markers were labeled in the respective mass range.

    Journal: Molecules

    Article Title: Lectin-Based Affinity Enrichment and Characterization of N-Glycoproteins from Human Tear Film by Mass Spectrometry

    doi: 10.3390/molecules28020648

    Figure Lengend Snippet: The 1D gel electrophoresis and glycoprotein/Coomassie staining of enriched glycoproteins from human tear sample pools. Both images illustrate the eluate as well as the flow-through (FT) fractions of the 3L multi-lectin column (combination of ConA, JAC, and WGA) and the 4L multi-lectin column system (combination of ConA, JAC, WGA, and UEA I). Prior to affinity enrichment, the tear sample pools were partially pre-treated with PNGase F to release the N-glycans from the glycoproteins. An untreated tear sample pool (50 µg) was also included to match the enriched glycoprotein spots with the native tear film proteome. In addition, a glycosylated protein standard (horseradish peroxidase, +CTRL), as well as a non-glycosylated protein standard (soybean trypsin inhibitor, -CTRL), were either included as a positive or negative control in this experiment. ( A ) Glycoprotein staining of the 1D gel which specifically colored the glycan motifs of the tear film glycoproteins/protein standards as magenta stained protein bands. ( B ) Coomassie protein staining of the 1D gel to visualize all the proteins as brilliant blue protein bands. The most abundant protein markers were labeled in the respective mass range.

    Article Snippet: For the glycoprotein/glycopeptide enrichment of the pooled tear film samples (see method ), we used the agarose-bound lectins concanavalin (ConA), wheat germ agglutinin (WGA), jacalin (JAC), and ulex europaeus agglutinin I (UEA I) from the manufacturer Vector Laboratories (Burlingame, CA, USA).

    Techniques: Nucleic Acid Electrophoresis, Staining, Negative Control, Labeling

    List of  lectins  and their respective glycan specificity.

    Journal: Molecules

    Article Title: Lectin-Based Affinity Enrichment and Characterization of N-Glycoproteins from Human Tear Film by Mass Spectrometry

    doi: 10.3390/molecules28020648

    Figure Lengend Snippet: List of lectins and their respective glycan specificity.

    Article Snippet: For the glycoprotein/glycopeptide enrichment of the pooled tear film samples (see method ), we used the agarose-bound lectins concanavalin (ConA), wheat germ agglutinin (WGA), jacalin (JAC), and ulex europaeus agglutinin I (UEA I) from the manufacturer Vector Laboratories (Burlingame, CA, USA).

    Techniques:

    (A) Expression of Meox2 mRNA in the most lateral region (rounded brackets) of presomitic mesoderm at stage 14 (dorsal view and transverse section), 16 (dorsal view), 17/18 (dorsal view and transverse section) and 23 (transverse section). Dotted lines indicate the position of the medial and lateral population of myogenic cells. Vertical lines define the limit between anterior and trunk region. Nc, notochord. (B) Lineage tracing experiments: embryos were injected with WGA-rhodamine (red) in the most lateral presomitic mesoderm (a) or co-injected with WGA-fluorescein (green) in the lateral presomitic mesoderm (c) at stage 13. Transverse sections of the embryo at the trunk level and at stages 18 or 23; embryo injected with WGA-rhodamine and submitted to indirect immunofluorescence with 12/101 antibody followed by secondary Alexa fluor 488 anti-mouse antibody (green) (b) or injected with the both tracers (d, e and f). WGA-rhodamine fluorescence (d), WGA-fluorescein fluorescence (e), merge (f). Dotted lines indicate the bilateral symmetry plan. In (a) and (c): Vertical lines define the limit between anterior and trunk region. (a) and (c) dorsal views, anterior side on the left. (C, D and E). Ablation experiments of presomitic mesoderm at stage 14. After microdissection experiments, embryos were fixed at stage 19 or at the tailbud stage to analyze meox2 and pax3 expression respectively. (C) sham-operated embryos, ectoderm was incised at the lateral level (line), ectoderm and mesoderm were separated from each other on the lateral side, but mesoderm was not removed. (D) Same operation on the mediolateral level but superficial mesoderm was removed (hatched zone) (E) Same operation on the lateral level but superficial mesoderm was removed (hatched zone). Brackets show the axis level corresponding to incision. For complete statistical data, see supporting information, .

    Journal: PLoS ONE

    Article Title: Mef2d Acts Upstream of Muscle Identity Genes and Couples Lateral Myogenesis to Dermomyotome Formation in Xenopus laevis

    doi: 10.1371/journal.pone.0052359

    Figure Lengend Snippet: (A) Expression of Meox2 mRNA in the most lateral region (rounded brackets) of presomitic mesoderm at stage 14 (dorsal view and transverse section), 16 (dorsal view), 17/18 (dorsal view and transverse section) and 23 (transverse section). Dotted lines indicate the position of the medial and lateral population of myogenic cells. Vertical lines define the limit between anterior and trunk region. Nc, notochord. (B) Lineage tracing experiments: embryos were injected with WGA-rhodamine (red) in the most lateral presomitic mesoderm (a) or co-injected with WGA-fluorescein (green) in the lateral presomitic mesoderm (c) at stage 13. Transverse sections of the embryo at the trunk level and at stages 18 or 23; embryo injected with WGA-rhodamine and submitted to indirect immunofluorescence with 12/101 antibody followed by secondary Alexa fluor 488 anti-mouse antibody (green) (b) or injected with the both tracers (d, e and f). WGA-rhodamine fluorescence (d), WGA-fluorescein fluorescence (e), merge (f). Dotted lines indicate the bilateral symmetry plan. In (a) and (c): Vertical lines define the limit between anterior and trunk region. (a) and (c) dorsal views, anterior side on the left. (C, D and E). Ablation experiments of presomitic mesoderm at stage 14. After microdissection experiments, embryos were fixed at stage 19 or at the tailbud stage to analyze meox2 and pax3 expression respectively. (C) sham-operated embryos, ectoderm was incised at the lateral level (line), ectoderm and mesoderm were separated from each other on the lateral side, but mesoderm was not removed. (D) Same operation on the mediolateral level but superficial mesoderm was removed (hatched zone) (E) Same operation on the lateral level but superficial mesoderm was removed (hatched zone). Brackets show the axis level corresponding to incision. For complete statistical data, see supporting information, .

    Article Snippet: Stage 13 embryos were injected using pulled-glass capillaries either with WGA-rhodamine (dissolved at 200 µg/ml, Vector laboratories) to be analysed at stage 18 or with both WGA-rhodamine and -fluorescein (Vector laboratories) to be analysed at stage 23.

    Techniques: Expressing, Injection, Immunofluorescence, Fluorescence, Laser Capture Microdissection