sba  (Vector Laboratories)


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    Vector Laboratories sba
    Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sba  (Vector Laboratories)


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    Vector Laboratories sba
    Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    soybean agglutinin sba  (Vector Laboratories)


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    Vector Laboratories soybean agglutinin sba
    List of antibodies used for staining.
    Soybean Agglutinin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts"

    Article Title: Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0007475

    List of antibodies used for staining.
    Figure Legend Snippet: List of antibodies used for staining.

    Techniques Used: Staining, Plasmid Preparation

    soybean agglutinin  (Vector Laboratories)


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    Vector Laboratories soybean agglutinin
    Soybean Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sba  (Vector Laboratories)


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    Vector Laboratories sba
    Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sba  (Vector Laboratories)


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    Vector Laboratories sba
    Lectin-biotin (-b) conjugates, 5 μg/mL final concentration, and streptavidin-DyLight488 reporter were applied. See Results text for preabsorption protocols. All panels show single widefield images of cross sections of AOs; RGB camera, 10x 0.3 NA lens. Similar image processing was applied to all panels, including deblurring and increased contrast. B: Barb-like protrusions from transitional epithelial cells of AO interior wall. EN: Electrosensory neuroepithelium. F: Flattened epithelial cells of the deep interior ampulla wall in AOs. P: Papillas lining the neck and pore. ( A <t>)</t> <t>WGA-biotin</t> labeled gel in the lumen of AOs, the EN, the interior epithelium of the ampulla wall, neck, and pore, and in the skin’s striated ectoderm. ( B ) Background fluorescence was low when WGA was omitted, but reporter was applied. EN: Autofluorescence from EN receptor cells (see ). ( C ) Nil label when WGA-biotin was preabsorbed (preabs.) with chitin hydrolysate (Chitin Hy.). ( D ) AO label like A when WGA-biotin was preabsorbed with N-acetyl-D-galactosamine (GalNAc). ( E ) <t>SBA-biotin</t> labeled AOs and luminal gel. ( F ) Label by SBA-biotin was abolished, as expected, when it was preabsorbed with N-acetyl-D-galactosamine, its preferred ligand. Also, B1 shows labeling of AOs, similar to E , by SBA-biotin preabsorbed with chitin hydrolysate.
    Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    sba - by Bioz Stars, 2024-05
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    1) Product Images from "Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon"

    Article Title: Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0276854

    Lectin-biotin (-b) conjugates, 5 μg/mL final concentration, and streptavidin-DyLight488 reporter were applied. See Results text for preabsorption protocols. All panels show single widefield images of cross sections of AOs; RGB camera, 10x 0.3 NA lens. Similar image processing was applied to all panels, including deblurring and increased contrast. B: Barb-like protrusions from transitional epithelial cells of AO interior wall. EN: Electrosensory neuroepithelium. F: Flattened epithelial cells of the deep interior ampulla wall in AOs. P: Papillas lining the neck and pore. ( A ) WGA-biotin labeled gel in the lumen of AOs, the EN, the interior epithelium of the ampulla wall, neck, and pore, and in the skin’s striated ectoderm. ( B ) Background fluorescence was low when WGA was omitted, but reporter was applied. EN: Autofluorescence from EN receptor cells (see ). ( C ) Nil label when WGA-biotin was preabsorbed (preabs.) with chitin hydrolysate (Chitin Hy.). ( D ) AO label like A when WGA-biotin was preabsorbed with N-acetyl-D-galactosamine (GalNAc). ( E ) SBA-biotin labeled AOs and luminal gel. ( F ) Label by SBA-biotin was abolished, as expected, when it was preabsorbed with N-acetyl-D-galactosamine, its preferred ligand. Also, B1 shows labeling of AOs, similar to E , by SBA-biotin preabsorbed with chitin hydrolysate.
    Figure Legend Snippet: Lectin-biotin (-b) conjugates, 5 μg/mL final concentration, and streptavidin-DyLight488 reporter were applied. See Results text for preabsorption protocols. All panels show single widefield images of cross sections of AOs; RGB camera, 10x 0.3 NA lens. Similar image processing was applied to all panels, including deblurring and increased contrast. B: Barb-like protrusions from transitional epithelial cells of AO interior wall. EN: Electrosensory neuroepithelium. F: Flattened epithelial cells of the deep interior ampulla wall in AOs. P: Papillas lining the neck and pore. ( A ) WGA-biotin labeled gel in the lumen of AOs, the EN, the interior epithelium of the ampulla wall, neck, and pore, and in the skin’s striated ectoderm. ( B ) Background fluorescence was low when WGA was omitted, but reporter was applied. EN: Autofluorescence from EN receptor cells (see ). ( C ) Nil label when WGA-biotin was preabsorbed (preabs.) with chitin hydrolysate (Chitin Hy.). ( D ) AO label like A when WGA-biotin was preabsorbed with N-acetyl-D-galactosamine (GalNAc). ( E ) SBA-biotin labeled AOs and luminal gel. ( F ) Label by SBA-biotin was abolished, as expected, when it was preabsorbed with N-acetyl-D-galactosamine, its preferred ligand. Also, B1 shows labeling of AOs, similar to E , by SBA-biotin preabsorbed with chitin hydrolysate.

    Techniques Used: Concentration Assay, Labeling, Fluorescence

    Transverse cross sections (10 μ) of EN were labeled with lectins WGA ( A, B ) or SBA ( C–E ). Tissue fixation or lectin conjugation were varied. Images show flattened projections from widefield partial stacks, with an EN’s apical face ‘up’. The nucleus (n) or cytoplasm (c) of support (s) or receptor (r) cells are indicated. Nuclei of support cells are more apical than nuclei of receptor cells . BL: Basal lamina. L: Lumen of AO. p: Pedicel of support cell . v: Void containing afferent branches . ( A, C, D ) ‘Hard’ fixation for 24 h preserved the apical microvilli (μv) on EN support cells, labeled using lectin-fluor conjugates; monochrome camera. ( B, E ) Brief (45 min) fixation, and label with lectin-biotin (-b) conjugates using streptavidin-DyLight488 reporter; RGB camera. ( A1–A5 ) Triple label with WGA-CF488A (green, Biotium, 2 μg/mL), and phalloidin-CF594 (Phal, red, Biotium), and DAPI (blue). ( A1–3 ) WGA label. ( A2 ) Enlarged part of A1 , resampled 4x. A support cell (S) was also marked on other panels of A . Arrows: Apical face of receptor cell (R). ( A3 ) WGA label with DAPI+ nuclei. Inset: Local patch of WGA+ microvilli in a single image from another section, resampled 4x. ( A4 ) Phalloidin (Phal) labeled apical microvilli (μv) on support cells. ( A5 ) All 3 labels were superimposed. Yellow color of apical microvilli on support cells indicated co-label by WGA and phalloidin. ( B ) WGA-biotin (WGA-b) label of EN, 1 μg/mL; stack of 6 images. ( C, D ) SBA-fluorescein label (Vector, 65 μg/mL). ( C ) This ampullary organ, its gel, and skin were labeled by SBA, which labeled the EN more brightly than in ampulla wall (W), and labeled the apical (Ap) surface of all AO epithelia. SE: Striated ectoderm. ( D ) SBA label of EN. Inset: Expanded view of SBA+ apical microvilli on support cells. ( E ) SBA-biotin label of EN, 5 μg/mL; stack of 3 images. ( A, D ) 60x lens, 0.5 μ stack steps. ( B, E ) 100x lens, 1 μ stack steps. ( C ) 10x 0.45 NA lens.
    Figure Legend Snippet: Transverse cross sections (10 μ) of EN were labeled with lectins WGA ( A, B ) or SBA ( C–E ). Tissue fixation or lectin conjugation were varied. Images show flattened projections from widefield partial stacks, with an EN’s apical face ‘up’. The nucleus (n) or cytoplasm (c) of support (s) or receptor (r) cells are indicated. Nuclei of support cells are more apical than nuclei of receptor cells . BL: Basal lamina. L: Lumen of AO. p: Pedicel of support cell . v: Void containing afferent branches . ( A, C, D ) ‘Hard’ fixation for 24 h preserved the apical microvilli (μv) on EN support cells, labeled using lectin-fluor conjugates; monochrome camera. ( B, E ) Brief (45 min) fixation, and label with lectin-biotin (-b) conjugates using streptavidin-DyLight488 reporter; RGB camera. ( A1–A5 ) Triple label with WGA-CF488A (green, Biotium, 2 μg/mL), and phalloidin-CF594 (Phal, red, Biotium), and DAPI (blue). ( A1–3 ) WGA label. ( A2 ) Enlarged part of A1 , resampled 4x. A support cell (S) was also marked on other panels of A . Arrows: Apical face of receptor cell (R). ( A3 ) WGA label with DAPI+ nuclei. Inset: Local patch of WGA+ microvilli in a single image from another section, resampled 4x. ( A4 ) Phalloidin (Phal) labeled apical microvilli (μv) on support cells. ( A5 ) All 3 labels were superimposed. Yellow color of apical microvilli on support cells indicated co-label by WGA and phalloidin. ( B ) WGA-biotin (WGA-b) label of EN, 1 μg/mL; stack of 6 images. ( C, D ) SBA-fluorescein label (Vector, 65 μg/mL). ( C ) This ampullary organ, its gel, and skin were labeled by SBA, which labeled the EN more brightly than in ampulla wall (W), and labeled the apical (Ap) surface of all AO epithelia. SE: Striated ectoderm. ( D ) SBA label of EN. Inset: Expanded view of SBA+ apical microvilli on support cells. ( E ) SBA-biotin label of EN, 5 μg/mL; stack of 3 images. ( A, D ) 60x lens, 0.5 μ stack steps. ( B, E ) 100x lens, 1 μ stack steps. ( C ) 10x 0.45 NA lens.

    Techniques Used: Labeling, Conjugation Assay, Plasmid Preparation

    ( A1 , B1 ) Frozen 10 μ cross sections of Polyodon AOs were labeled with biotinylated lectins and streptavidin-DyLight488 fluorescent reporter (Vector). Single raw widefield images, 10x 0.3 NA lens, RGB camera. ( A1 ) WGA-biotin (WGA-b), 5 μg/mL. ( B1 ) SBA-biotin, 5 μg/mL, preabsorbed with chitin hydrolysate solution. -b: Biotinylated lectin. BL: Basal Lamina. D: Dermis between AOs. EN: Electrosensory neuroepithelium. F: Flattened smooth ampulla wall epithelium, deep in AOs, distal of the EN. H: Hypodermis deep to AOs. Red arrowheads: Limits of an EN. SE: Striated ectoderm; a double arrow marks its thickness. Yellow lines: Paths of profile plots, identified by their panel codes. ( A2 ) Profile plot of pixel gray values (8-bit scale) along the left AO in A1 , and continuing outside its pore (red * ). Apical EN: Maximal emission near the apical face of an EN. ( A1 , A2 ) Orange ■: Gap between gel and EN; see Discussion . ( A3 ) Profile plot from left to right across a gel-filled partial AO in A1 ; maximal fluorescence was from the apical face of non-EN epithelia of the ampulla walls. ( B2 , B3 ) Profile plots from B1 . SBA+ gel was disturbed in the neck and pore of these AOs.
    Figure Legend Snippet: ( A1 , B1 ) Frozen 10 μ cross sections of Polyodon AOs were labeled with biotinylated lectins and streptavidin-DyLight488 fluorescent reporter (Vector). Single raw widefield images, 10x 0.3 NA lens, RGB camera. ( A1 ) WGA-biotin (WGA-b), 5 μg/mL. ( B1 ) SBA-biotin, 5 μg/mL, preabsorbed with chitin hydrolysate solution. -b: Biotinylated lectin. BL: Basal Lamina. D: Dermis between AOs. EN: Electrosensory neuroepithelium. F: Flattened smooth ampulla wall epithelium, deep in AOs, distal of the EN. H: Hypodermis deep to AOs. Red arrowheads: Limits of an EN. SE: Striated ectoderm; a double arrow marks its thickness. Yellow lines: Paths of profile plots, identified by their panel codes. ( A2 ) Profile plot of pixel gray values (8-bit scale) along the left AO in A1 , and continuing outside its pore (red * ). Apical EN: Maximal emission near the apical face of an EN. ( A1 , A2 ) Orange ■: Gap between gel and EN; see Discussion . ( A3 ) Profile plot from left to right across a gel-filled partial AO in A1 ; maximal fluorescence was from the apical face of non-EN epithelia of the ampulla walls. ( B2 , B3 ) Profile plots from B1 . SBA+ gel was disturbed in the neck and pore of these AOs.

    Techniques Used: Labeling, Plasmid Preparation, Fluorescence

    b: Biotinylated lectin. B: Barbed wall cells. c: Capillaries near AO. D: Dermis. EN: Electrosensory neuroepithelium. F: Flattened cells of deep ampulla wall. L: Lumen of AO. m: Melanin-containing outer fibrous layer on AOs. P: Papilla cells lining neck and pore. Phal: Phalloidin label. SE: Striated ectoderm. W: Non-EN ampulla wall epithelium. ( A) Brightfield cross-section of AO; unstained, 10x phase lens. Partial luminal gel remained at the EN. ( B ) Cross section of an AO and adjacent skin stained with phalloidin (orange) and WGA-biotin (green). *: AO gel with small particles was visible outside the pore. ( C, D ) Parallel views of the interior wall of AOs labeled with anti-S100-ß ( C ) or WGA-CF488A ( D ), showing different cells types of the ampulla wall epithelium, including flattened cells (F) deep in AOs, transitional cells with barb-like protrusions (B), and papilla cells (P) in the neck and pore. ( E–H ) Closeup images of cross sections of ampulla wall showing vesicles in wall epithelial cells labeled by a lectin; see keys. Double arrows: Thickness of wall epithelium. ( E ) WGA-b, 1 μg/mL. ( F ) ConA-b, 5 μg/mL. ( G ) RCA-b, 2.5 μg/mL. ( H ) SBA-b, 5 μg/mL. Arrow: Apical vesicle labeled by SBA-biotin. *: Barb-like SBA+ protrusion from wall cell. (E , H ) 100x lens. ( F , G ) 40x lens. ( I ) SBA-biotin label of horizontally sectioned ampulla walls; stack projection of a 50 μ section parallel to skin. Blue arrow: En face view of SBA-b+ apical surface labeling on epithelial cells, in a tilted part of the ampulla wall, enlarged in inset 1. Green arrow: SBA-b+ apical label of cross-sectioned epithelial cells of ampulla wall. Red arrow: Sectioned SBA-b+ barb-like protrusions from wall cells, enlarged in inset 2 (resampled 4x).
    Figure Legend Snippet: b: Biotinylated lectin. B: Barbed wall cells. c: Capillaries near AO. D: Dermis. EN: Electrosensory neuroepithelium. F: Flattened cells of deep ampulla wall. L: Lumen of AO. m: Melanin-containing outer fibrous layer on AOs. P: Papilla cells lining neck and pore. Phal: Phalloidin label. SE: Striated ectoderm. W: Non-EN ampulla wall epithelium. ( A) Brightfield cross-section of AO; unstained, 10x phase lens. Partial luminal gel remained at the EN. ( B ) Cross section of an AO and adjacent skin stained with phalloidin (orange) and WGA-biotin (green). *: AO gel with small particles was visible outside the pore. ( C, D ) Parallel views of the interior wall of AOs labeled with anti-S100-ß ( C ) or WGA-CF488A ( D ), showing different cells types of the ampulla wall epithelium, including flattened cells (F) deep in AOs, transitional cells with barb-like protrusions (B), and papilla cells (P) in the neck and pore. ( E–H ) Closeup images of cross sections of ampulla wall showing vesicles in wall epithelial cells labeled by a lectin; see keys. Double arrows: Thickness of wall epithelium. ( E ) WGA-b, 1 μg/mL. ( F ) ConA-b, 5 μg/mL. ( G ) RCA-b, 2.5 μg/mL. ( H ) SBA-b, 5 μg/mL. Arrow: Apical vesicle labeled by SBA-biotin. *: Barb-like SBA+ protrusion from wall cell. (E , H ) 100x lens. ( F , G ) 40x lens. ( I ) SBA-biotin label of horizontally sectioned ampulla walls; stack projection of a 50 μ section parallel to skin. Blue arrow: En face view of SBA-b+ apical surface labeling on epithelial cells, in a tilted part of the ampulla wall, enlarged in inset 1. Green arrow: SBA-b+ apical label of cross-sectioned epithelial cells of ampulla wall. Red arrow: Sectioned SBA-b+ barb-like protrusions from wall cells, enlarged in inset 2 (resampled 4x).

    Techniques Used: Staining, Labeling

    ( A ) Cross section of skin showing WGA-CF488A label in the neck and pore of AOs, including a deep AO (left) and an adjacent small AO; 20x 0.5 NA lens. P: Papilla cells covered a ~250 μ-deep band in the neck and pore. * : Transition from papilla cells to flattened ampulla wall cells (W). SE: Striated ectoderm. Small AO: En face view of the interior apical surface of its electrosensory neuroepithelium (EN) and ampulla wall, which included papilla cells (P). Arrow 1: 236 μ depth of the papilla cell band in the deep AO (left side). Arrow 2: 223 μ maximal wall depth of the small AO. Arrow 3: 286 μ total depth of the small AO. Pink construction lines show dimensions of the small AO’s tapered ampulla wall, including its height (142 μ), maximum internal width (218 μ), and the neck’s ID (51 μ), for a model of gel acceleration (Tab 1 in ). ( B–D ) Biotinylated (-b) lectins and streptavidin-DyLight488 reporter were used. ( B ) Stack image parallel to the neck interior of an AO, showing WGA-b+ papillas (P) in the neck (N), and that large WGA-b+ papillas, almost parallel to the wall, continued into the distal ampulla; 10x lens. L: Lumen of AO. ( C ) Lectin DBA-b (preabsorbed with chitin hydrolysate solution) labeled the interior epithelium of this AO, including papilla cells (P) in the neck (N). DBA-b+ gel was visible in the AO’s lumen; 10x lens. ( D ) Cross section of short papilla cells (P) labeled by SBA-b in the neck / pore of an AO; single image, 100x lens.
    Figure Legend Snippet: ( A ) Cross section of skin showing WGA-CF488A label in the neck and pore of AOs, including a deep AO (left) and an adjacent small AO; 20x 0.5 NA lens. P: Papilla cells covered a ~250 μ-deep band in the neck and pore. * : Transition from papilla cells to flattened ampulla wall cells (W). SE: Striated ectoderm. Small AO: En face view of the interior apical surface of its electrosensory neuroepithelium (EN) and ampulla wall, which included papilla cells (P). Arrow 1: 236 μ depth of the papilla cell band in the deep AO (left side). Arrow 2: 223 μ maximal wall depth of the small AO. Arrow 3: 286 μ total depth of the small AO. Pink construction lines show dimensions of the small AO’s tapered ampulla wall, including its height (142 μ), maximum internal width (218 μ), and the neck’s ID (51 μ), for a model of gel acceleration (Tab 1 in ). ( B–D ) Biotinylated (-b) lectins and streptavidin-DyLight488 reporter were used. ( B ) Stack image parallel to the neck interior of an AO, showing WGA-b+ papillas (P) in the neck (N), and that large WGA-b+ papillas, almost parallel to the wall, continued into the distal ampulla; 10x lens. L: Lumen of AO. ( C ) Lectin DBA-b (preabsorbed with chitin hydrolysate solution) labeled the interior epithelium of this AO, including papilla cells (P) in the neck (N). DBA-b+ gel was visible in the AO’s lumen; 10x lens. ( D ) Cross section of short papilla cells (P) labeled by SBA-b in the neck / pore of an AO; single image, 100x lens.

    Techniques Used: Labeling

    soybean agglutinins sba  (Vector Laboratories)


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    Vector Laboratories soybean agglutinins sba
    Surface glycan motifs of Tn-bearing LL/2 and WT cells determined by lectin recognition. LL/2 cells were stained with plant lectins and analyzed by flow cytometry. ( A ) Representative gating strategy. B-H) Histogram and median fluorescence intensity (MFI) are shown for LL/2-Tn + -H12 (green) and LL/2-Tn + -F9 (pink) relative to WT LL/2 cells for VVL ( B ), JAC ( C ), HPA ( D ), <t>SBA</t> ( E ), DBA ( F ), MAL II ( G ) <t>and</t> <t>SNA</t> ( H ). Dotted histograms represent the control condition consisting of cells incubated only in the presence of streptavidin-FITC. The specificity of lectins is shown in . Asterisks represent significant differences (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.00001).
    Soybean Agglutinins Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soybean agglutinins sba/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    Images

    1) Product Images from "Lung Tumor Cells with Different Tn Antigen Expression Present Distinctive Immunomodulatory Properties"

    Article Title: Lung Tumor Cells with Different Tn Antigen Expression Present Distinctive Immunomodulatory Properties

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms231912047

    Surface glycan motifs of Tn-bearing LL/2 and WT cells determined by lectin recognition. LL/2 cells were stained with plant lectins and analyzed by flow cytometry. ( A ) Representative gating strategy. B-H) Histogram and median fluorescence intensity (MFI) are shown for LL/2-Tn + -H12 (green) and LL/2-Tn + -F9 (pink) relative to WT LL/2 cells for VVL ( B ), JAC ( C ), HPA ( D ), SBA ( E ), DBA ( F ), MAL II ( G ) and SNA ( H ). Dotted histograms represent the control condition consisting of cells incubated only in the presence of streptavidin-FITC. The specificity of lectins is shown in . Asterisks represent significant differences (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.00001).
    Figure Legend Snippet: Surface glycan motifs of Tn-bearing LL/2 and WT cells determined by lectin recognition. LL/2 cells were stained with plant lectins and analyzed by flow cytometry. ( A ) Representative gating strategy. B-H) Histogram and median fluorescence intensity (MFI) are shown for LL/2-Tn + -H12 (green) and LL/2-Tn + -F9 (pink) relative to WT LL/2 cells for VVL ( B ), JAC ( C ), HPA ( D ), SBA ( E ), DBA ( F ), MAL II ( G ) and SNA ( H ). Dotted histograms represent the control condition consisting of cells incubated only in the presence of streptavidin-FITC. The specificity of lectins is shown in . Asterisks represent significant differences (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.00001).

    Techniques Used: Staining, Flow Cytometry, Fluorescence, Incubation

    soybean agglutinin  (Vector Laboratories)


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    Vector Laboratories soybean agglutinin
    Soybean Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    soybean agglutinin sba unconjugated  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
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    Vector Laboratories soybean agglutinin sba unconjugated
    Soybean Agglutinin Sba Unconjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soybean agglutinin sba unconjugated/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    sba  (Vector Laboratories)


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    Vector Laboratories sba
    Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    soy bean agglutinin  (Vector Laboratories)


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  • 93

    Structured Review

    Vector Laboratories soy bean agglutinin
    Soy Bean Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soy bean agglutinin/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    Vector Laboratories sba
    Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sba/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sba - by Bioz Stars, 2024-05
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    93
    Vector Laboratories soybean agglutinin sba
    List of antibodies used for staining.
    Soybean Agglutinin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soybean agglutinin sba/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    93
    Vector Laboratories soybean agglutinin
    List of antibodies used for staining.
    Soybean Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Vector Laboratories soybean agglutinins sba
    Surface glycan motifs of Tn-bearing LL/2 and WT cells determined by lectin recognition. LL/2 cells were stained with plant lectins and analyzed by flow cytometry. ( A ) Representative gating strategy. B-H) Histogram and median fluorescence intensity (MFI) are shown for LL/2-Tn + -H12 (green) and LL/2-Tn + -F9 (pink) relative to WT LL/2 cells for VVL ( B ), JAC ( C ), HPA ( D ), <t>SBA</t> ( E ), DBA ( F ), MAL II ( G ) <t>and</t> <t>SNA</t> ( H ). Dotted histograms represent the control condition consisting of cells incubated only in the presence of streptavidin-FITC. The specificity of lectins is shown in . Asterisks represent significant differences (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.00001).
    Soybean Agglutinins Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soybean agglutinins sba/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    soybean agglutinins sba - by Bioz Stars, 2024-05
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    Vector Laboratories soybean agglutinin sba unconjugated
    Surface glycan motifs of Tn-bearing LL/2 and WT cells determined by lectin recognition. LL/2 cells were stained with plant lectins and analyzed by flow cytometry. ( A ) Representative gating strategy. B-H) Histogram and median fluorescence intensity (MFI) are shown for LL/2-Tn + -H12 (green) and LL/2-Tn + -F9 (pink) relative to WT LL/2 cells for VVL ( B ), JAC ( C ), HPA ( D ), <t>SBA</t> ( E ), DBA ( F ), MAL II ( G ) <t>and</t> <t>SNA</t> ( H ). Dotted histograms represent the control condition consisting of cells incubated only in the presence of streptavidin-FITC. The specificity of lectins is shown in . Asterisks represent significant differences (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.00001).
    Soybean Agglutinin Sba Unconjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soybean agglutinin sba unconjugated/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    soybean agglutinin sba unconjugated - by Bioz Stars, 2024-05
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    93
    Vector Laboratories soy bean agglutinin
    Surface glycan motifs of Tn-bearing LL/2 and WT cells determined by lectin recognition. LL/2 cells were stained with plant lectins and analyzed by flow cytometry. ( A ) Representative gating strategy. B-H) Histogram and median fluorescence intensity (MFI) are shown for LL/2-Tn + -H12 (green) and LL/2-Tn + -F9 (pink) relative to WT LL/2 cells for VVL ( B ), JAC ( C ), HPA ( D ), <t>SBA</t> ( E ), DBA ( F ), MAL II ( G ) <t>and</t> <t>SNA</t> ( H ). Dotted histograms represent the control condition consisting of cells incubated only in the presence of streptavidin-FITC. The specificity of lectins is shown in . Asterisks represent significant differences (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.00001).
    Soy Bean Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soy bean agglutinin/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    soy bean agglutinin - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    List of antibodies used for staining.

    Journal: PLoS ONE

    Article Title: Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts

    doi: 10.1371/journal.pone.0007475

    Figure Lengend Snippet: List of antibodies used for staining.

    Article Snippet: Soybean agglutinin (SBA) , B-1015 , Biotinylated lectin , Vector Laboratories.

    Techniques: Staining, Plasmid Preparation

    Surface glycan motifs of Tn-bearing LL/2 and WT cells determined by lectin recognition. LL/2 cells were stained with plant lectins and analyzed by flow cytometry. ( A ) Representative gating strategy. B-H) Histogram and median fluorescence intensity (MFI) are shown for LL/2-Tn + -H12 (green) and LL/2-Tn + -F9 (pink) relative to WT LL/2 cells for VVL ( B ), JAC ( C ), HPA ( D ), SBA ( E ), DBA ( F ), MAL II ( G ) and SNA ( H ). Dotted histograms represent the control condition consisting of cells incubated only in the presence of streptavidin-FITC. The specificity of lectins is shown in . Asterisks represent significant differences (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.00001).

    Journal: International Journal of Molecular Sciences

    Article Title: Lung Tumor Cells with Different Tn Antigen Expression Present Distinctive Immunomodulatory Properties

    doi: 10.3390/ijms231912047

    Figure Lengend Snippet: Surface glycan motifs of Tn-bearing LL/2 and WT cells determined by lectin recognition. LL/2 cells were stained with plant lectins and analyzed by flow cytometry. ( A ) Representative gating strategy. B-H) Histogram and median fluorescence intensity (MFI) are shown for LL/2-Tn + -H12 (green) and LL/2-Tn + -F9 (pink) relative to WT LL/2 cells for VVL ( B ), JAC ( C ), HPA ( D ), SBA ( E ), DBA ( F ), MAL II ( G ) and SNA ( H ). Dotted histograms represent the control condition consisting of cells incubated only in the presence of streptavidin-FITC. The specificity of lectins is shown in . Asterisks represent significant differences (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.00001).

    Article Snippet: Wild type (WT) and Tn + LL/2 tumor cells were stained with anti-Tn antibody 83D4 or the following lectins (Vector Labs, Newark, CA, USA): Helix pomatia lectin (HPA), VVL, Sambucus Nigra lectin (SNA), Maackia amurensis II lectin (MAL II),), Dolichos biflorus agglutinin (DBA) and Soybean agglutinins (SBA) in PBS containing 2% BSA, 0.02% NaN 3 , or MGL1-Fc and MGL2-Fc in Hanks’ Balanced Salt Solution (HBSS) with 0.5% BSA, for 30 min at 4 °C.

    Techniques: Staining, Flow Cytometry, Fluorescence, Incubation