1 2 dilauroyl sn glycerol  (Echelon Biosciences)


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    Structured Review

    Echelon Biosciences 1 2 dilauroyl sn glycerol
    1 2 Dilauroyl Sn Glycerol, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 2 dilauroyl sn glycerol/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1 2 dilauroyl sn glycerol - by Bioz Stars, 2024-05
    90/100 stars

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    1 2 dilauroyl sn glycerol  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
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    Structured Review

    Echelon Biosciences 1 2 dilauroyl sn glycerol
    1 2 Dilauroyl Sn Glycerol, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 2 dilauroyl sn glycerol/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1 2 dilauroyl sn glycerol - by Bioz Stars, 2024-05
    90/100 stars

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    1 2 dilauroyl sn glycerol  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
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    Echelon Biosciences 1 2 dilauroyl sn glycerol
    The ER stress sensor PERK demonstrates lipid kinase activity in vitro and in vivo. (a) PERK was immunopurified from p110+/+ or p110α−/− MEFs, and lipid kinase activity was determined in an in vitro assay with phosphatidylinositol as a substrate. NRS, nonspecific rabbit serum. Thaps, thapsigargin. (b) In vitro lipid kinase assay with phosphatidylinositol as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK) or kinase-dead PERK (K618A). (c) In vitro lipid kinase assay with phosphatidylinositol as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK), kinase-dead PERK (K618A), p38, GSK3β, PKA C (500 ng of each kinase), and PI3K p85/p110 complex. (d) In vitro lipid kinase assay with phosphatidylinositol as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK) or recombinant PI3K and various phosphoinositide substrates. CBE, crude brain extract. Products were resolved by chromatography with propanol and acetic acid as the mobile phase. (e) The radioactive spot from the TLC plate in panel d (with CBE as a substrate) was excised, and PERK product was deacylated and then resolved by HPLC with 3H-labeled PI, PI4P, and PI4,5P2 as standards. (f) In vitro lipid kinase assay with diacylglycerol (DAG) as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK). <t>DAGC12,</t> <t>1,2-dilauroyl-sn-glycerol.</t> (g) In vitro lipid kinase assay with phosphatidylinositol (PI) or diacylglycerol (DAG) as a substrate and the recombinant catalytic domain of PERK in the presence or absence of recombinant PI3K p85α or with recombinant diacylglycerol kinase (DGK). DAGC14, 1,2-dimyristoyl-sn-glycerol; DAGC16, 1,2-dipalmitoyl-sn-glycerol; DAGC18, 1,2-distearoyl-sn-glycerol.
    1 2 Dilauroyl Sn Glycerol, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 2 dilauroyl sn glycerol/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1 2 dilauroyl sn glycerol - by Bioz Stars, 2024-05
    90/100 stars

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    1) Product Images from "PERK Utilizes Intrinsic Lipid Kinase Activity To Generate Phosphatidic Acid, Mediate Akt Activation, and Promote Adipocyte Differentiation"

    Article Title: PERK Utilizes Intrinsic Lipid Kinase Activity To Generate Phosphatidic Acid, Mediate Akt Activation, and Promote Adipocyte Differentiation

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.00063-12

    The ER stress sensor PERK demonstrates lipid kinase activity in vitro and in vivo. (a) PERK was immunopurified from p110+/+ or p110α−/− MEFs, and lipid kinase activity was determined in an in vitro assay with phosphatidylinositol as a substrate. NRS, nonspecific rabbit serum. Thaps, thapsigargin. (b) In vitro lipid kinase assay with phosphatidylinositol as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK) or kinase-dead PERK (K618A). (c) In vitro lipid kinase assay with phosphatidylinositol as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK), kinase-dead PERK (K618A), p38, GSK3β, PKA C (500 ng of each kinase), and PI3K p85/p110 complex. (d) In vitro lipid kinase assay with phosphatidylinositol as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK) or recombinant PI3K and various phosphoinositide substrates. CBE, crude brain extract. Products were resolved by chromatography with propanol and acetic acid as the mobile phase. (e) The radioactive spot from the TLC plate in panel d (with CBE as a substrate) was excised, and PERK product was deacylated and then resolved by HPLC with 3H-labeled PI, PI4P, and PI4,5P2 as standards. (f) In vitro lipid kinase assay with diacylglycerol (DAG) as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK). DAGC12, 1,2-dilauroyl-sn-glycerol. (g) In vitro lipid kinase assay with phosphatidylinositol (PI) or diacylglycerol (DAG) as a substrate and the recombinant catalytic domain of PERK in the presence or absence of recombinant PI3K p85α or with recombinant diacylglycerol kinase (DGK). DAGC14, 1,2-dimyristoyl-sn-glycerol; DAGC16, 1,2-dipalmitoyl-sn-glycerol; DAGC18, 1,2-distearoyl-sn-glycerol.
    Figure Legend Snippet: The ER stress sensor PERK demonstrates lipid kinase activity in vitro and in vivo. (a) PERK was immunopurified from p110+/+ or p110α−/− MEFs, and lipid kinase activity was determined in an in vitro assay with phosphatidylinositol as a substrate. NRS, nonspecific rabbit serum. Thaps, thapsigargin. (b) In vitro lipid kinase assay with phosphatidylinositol as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK) or kinase-dead PERK (K618A). (c) In vitro lipid kinase assay with phosphatidylinositol as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK), kinase-dead PERK (K618A), p38, GSK3β, PKA C (500 ng of each kinase), and PI3K p85/p110 complex. (d) In vitro lipid kinase assay with phosphatidylinositol as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK) or recombinant PI3K and various phosphoinositide substrates. CBE, crude brain extract. Products were resolved by chromatography with propanol and acetic acid as the mobile phase. (e) The radioactive spot from the TLC plate in panel d (with CBE as a substrate) was excised, and PERK product was deacylated and then resolved by HPLC with 3H-labeled PI, PI4P, and PI4,5P2 as standards. (f) In vitro lipid kinase assay with diacylglycerol (DAG) as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK). DAGC12, 1,2-dilauroyl-sn-glycerol. (g) In vitro lipid kinase assay with phosphatidylinositol (PI) or diacylglycerol (DAG) as a substrate and the recombinant catalytic domain of PERK in the presence or absence of recombinant PI3K p85α or with recombinant diacylglycerol kinase (DGK). DAGC14, 1,2-dimyristoyl-sn-glycerol; DAGC16, 1,2-dipalmitoyl-sn-glycerol; DAGC18, 1,2-distearoyl-sn-glycerol.

    Techniques Used: Activity Assay, In Vitro, In Vivo, Kinase Assay, Recombinant, Chromatography, Labeling

    p85 increases PERK lipid kinase activity and Akt activation. (a) Activation of Akt in response to ER stress in wild-type, p85/p85 double-knockout (DKO), or DKO MEFs reconstituted with wild-type p85α (+wt p85). Phosphorylated p-4EBP1 is indicated by the arrow. (b) In vitro lipid kinase with DAGC12 as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK) alone or in the presence of recombinant p85α wild type. The intensity of phosphorylated lipid product signal was measured using phosphor imaging screen and STORM scanner (graph shown).
    Figure Legend Snippet: p85 increases PERK lipid kinase activity and Akt activation. (a) Activation of Akt in response to ER stress in wild-type, p85/p85 double-knockout (DKO), or DKO MEFs reconstituted with wild-type p85α (+wt p85). Phosphorylated p-4EBP1 is indicated by the arrow. (b) In vitro lipid kinase with DAGC12 as a substrate and recombinant catalytic domain of PERK (GST-ΔNPERK) alone or in the presence of recombinant p85α wild type. The intensity of phosphorylated lipid product signal was measured using phosphor imaging screen and STORM scanner (graph shown).

    Techniques Used: Activity Assay, Activation Assay, Double Knockout, In Vitro, Recombinant, Imaging

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    Echelon Biosciences 1 2 dilauroyl sn glycerol
    1 2 Dilauroyl Sn Glycerol, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 2 dilauroyl sn glycerol/product/Echelon Biosciences
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1 2 dilauroyl sn glycerol - by Bioz Stars, 2024-05
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