l threonine  (Millipore)


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  • 95
    Name:
    L Threonine
    Description:

    Catalog Number:
    t8625
    Price:
    None
    Applications:
    L-threonine has been used for the selection of transformed cells. It has also been used to study the effects of amino acids in promoting food consumption in Drosophila melanogaster.
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    Structured Review

    Millipore l threonine
    L Threonine

    https://www.bioz.com/result/l threonine/product/Millipore
    Average 95 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    l threonine - by Bioz Stars, 2020-08
    95/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: A novel pathway of nutrient absorption in crustaceans: branchial amino acid uptake in the green shore crab (Carcinus maenas)
    Article Snippet: .. Then, excess of d -leucine, l -threonine or l -lysine (to give a final concentration of 20 µM, Sigma-Aldrich) were added to the bathing medium, and perfusions were conducted, as described above, in a posterior gill (gill 8). .. To test the sodium dependence of l -leucine transport, a low-sodium artificial haemolymph was made by replacing sodium chloride with choline chloride (Sigma-Aldrich) at a concentration of 470 mM to maintain osmolality.

    other:

    Article Title: Slow unloading leads to DNA-bound β2-sliding clamp accumulation in live Escherichia coli cells
    Article Snippet: One litre of M9 growth medium contains 10.5 g l−1 of autoclaved M9 broth (Sigma-Aldrich); 0.1 mM of autoclaved CaCl2 (Sigma-Aldrich); 0.1 mM of autoclaved MgSO4 (J.T.Baker); 0.3% of filter-sterilized glycerol (Sigma-Aldrich) as carbon source; 0.1 g l−1 of filter-sterilized five amino acids, namely L -threonine, L -leucine, L -proline, L -histidine and L -arginine (all from Sigma-Aldrich) and 10 μl of 0.5% filter-sterilized thiamine (Sigma-Aldrich).

    Purification:

    Article Title: Crystallization of Amino Acids on a 21-well Circular PMMA Platform using Metal-Assisted and Microwave-Accelerated Evaporative Crystallization
    Article Snippet: .. L-Histidine, L-Leucine, L-Serine, L-Threonine, and L-Valine were purchased from Sigma-Aldrich (USA) and were used without further purification. .. Hydrochloric acid (HCl) was obtained from Sigma-Aldrich (USA).

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  • 93
    Millipore wt1
    Absence of Npnt increases mesangial cell number. (A and B) Immunohistochemistry with (A) endothelial marker ERG and (B) podocyte marker <t>WT1</t> from sections of 6-week-old control (Ctl; Npnt f/f ) and mutant (Mut; Npnt f/f ; Six2-cre ) mice. Scale bars: 25 μ m. (C) Immunofluorescence with mesangial marker α 8 integrin, endothelial marker ERG, and nuclear marker DAPI in 6-week-old mice. (D and E) Quantification of (A and B) as the percentage of endothelial cells (ERG + ) and podocytes (WT1 + ) per total glomerular cells is shown; > 5000 cells were counted from n =3 mice of each genotype. Analysis with the unpaired t test (* P
    Wt1, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wt1/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    wt1 - by Bioz Stars, 2020-08
    93/100 stars
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    85
    Millipore serine threonine phosphatase inhibitors okadaic acid
    Effect of phosphatase inhibitors on PP1, PP2A, and PP2B enzymatic activity. Sperm were selected and re-suspended in NCM. Each phosphatase activity was measured in sperm extracts as indicated in Materials and Methods in the absence of inhibitors (black bars) and then in the presence of 10 nM <t>okadaic</t> acid (white bar); 40 µg/ml I2 (grey bar); 90 nM endothall (dotted bar); 0.1 nM okadaic acid (light grey bar); or 0.1 nM deltamethrin (striped bar). Results were obtained from five different donors and are expressed as the mean±SEM of forty measurements every 2 min. * p
    Serine Threonine Phosphatase Inhibitors Okadaic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/serine threonine phosphatase inhibitors okadaic acid/product/Millipore
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    serine threonine phosphatase inhibitors okadaic acid - by Bioz Stars, 2020-08
    85/100 stars
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    97
    Millipore raf proto oncogene serine threonine protein kinase raf 1 ras binding domain
    Comparison of effector-binding activity of GTP-bound wild-type, G12D, and G12V K-Ras4B 1–188 by use of <t>Raf-1</t> RBD-binding assay in the absence ( A ) and presence ( B ) of membrane fractions prepared from NIH 3T3 fibroblasts.
    Raf Proto Oncogene Serine Threonine Protein Kinase Raf 1 Ras Binding Domain, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/raf proto oncogene serine threonine protein kinase raf 1 ras binding domain/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    raf proto oncogene serine threonine protein kinase raf 1 ras binding domain - by Bioz Stars, 2020-08
    97/100 stars
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    Image Search Results


    Absence of Npnt increases mesangial cell number. (A and B) Immunohistochemistry with (A) endothelial marker ERG and (B) podocyte marker WT1 from sections of 6-week-old control (Ctl; Npnt f/f ) and mutant (Mut; Npnt f/f ; Six2-cre ) mice. Scale bars: 25 μ m. (C) Immunofluorescence with mesangial marker α 8 integrin, endothelial marker ERG, and nuclear marker DAPI in 6-week-old mice. (D and E) Quantification of (A and B) as the percentage of endothelial cells (ERG + ) and podocytes (WT1 + ) per total glomerular cells is shown; > 5000 cells were counted from n =3 mice of each genotype. Analysis with the unpaired t test (* P

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Nephronectin Regulates Mesangial Cell Adhesion and Behavior in Glomeruli

    doi: 10.1681/ASN.2017070752

    Figure Lengend Snippet: Absence of Npnt increases mesangial cell number. (A and B) Immunohistochemistry with (A) endothelial marker ERG and (B) podocyte marker WT1 from sections of 6-week-old control (Ctl; Npnt f/f ) and mutant (Mut; Npnt f/f ; Six2-cre ) mice. Scale bars: 25 μ m. (C) Immunofluorescence with mesangial marker α 8 integrin, endothelial marker ERG, and nuclear marker DAPI in 6-week-old mice. (D and E) Quantification of (A and B) as the percentage of endothelial cells (ERG + ) and podocytes (WT1 + ) per total glomerular cells is shown; > 5000 cells were counted from n =3 mice of each genotype. Analysis with the unpaired t test (* P

    Article Snippet: Miner), laminin (Sigma, 9393), WT1 (Millipore, 05–753), ERG (Abcam, ab92513), vinculin (SBT, sc-7649), paxillin (Abcam, ab32084), lotus tetragonolobus lectin (Vector Laboratories, B1325), and phalloidin 647 (Invitrogen).

    Techniques: Immunohistochemistry, Marker, CTL Assay, Mutagenesis, Mouse Assay, Immunofluorescence

    Effect of phosphatase inhibitors on PP1, PP2A, and PP2B enzymatic activity. Sperm were selected and re-suspended in NCM. Each phosphatase activity was measured in sperm extracts as indicated in Materials and Methods in the absence of inhibitors (black bars) and then in the presence of 10 nM okadaic acid (white bar); 40 µg/ml I2 (grey bar); 90 nM endothall (dotted bar); 0.1 nM okadaic acid (light grey bar); or 0.1 nM deltamethrin (striped bar). Results were obtained from five different donors and are expressed as the mean±SEM of forty measurements every 2 min. * p

    Journal: PLoS ONE

    Article Title: Protein Phosphatases Decrease Their Activity during Capacitation: A New Requirement for This Event

    doi: 10.1371/journal.pone.0081286

    Figure Lengend Snippet: Effect of phosphatase inhibitors on PP1, PP2A, and PP2B enzymatic activity. Sperm were selected and re-suspended in NCM. Each phosphatase activity was measured in sperm extracts as indicated in Materials and Methods in the absence of inhibitors (black bars) and then in the presence of 10 nM okadaic acid (white bar); 40 µg/ml I2 (grey bar); 90 nM endothall (dotted bar); 0.1 nM okadaic acid (light grey bar); or 0.1 nM deltamethrin (striped bar). Results were obtained from five different donors and are expressed as the mean±SEM of forty measurements every 2 min. * p

    Article Snippet: The serine-threonine phosphatase inhibitors okadaic acid, deltamethrin, and calmodulin were obtained from Calbiochem Corporation (La Jolla, CA).

    Techniques: Activity Assay

    Effect of protein phosphatase inhibitors on human sperm capacitation. Human sperm were incubated in NCM (A) or RCM (B) for 120 min, and the capacitation pattern was evaluated using the CTC assay, as described in the Materials and Methods . The inhibitors used were okadaic acid (black squares), deltamethrin (green diamonds), and endothall (blue triangles). Results were obtained from five different donors and were expressed as the mean±SEM of the percentage of B pattern. The arrow indicates the addition of BSA and bicarbonate. * p

    Journal: PLoS ONE

    Article Title: Protein Phosphatases Decrease Their Activity during Capacitation: A New Requirement for This Event

    doi: 10.1371/journal.pone.0081286

    Figure Lengend Snippet: Effect of protein phosphatase inhibitors on human sperm capacitation. Human sperm were incubated in NCM (A) or RCM (B) for 120 min, and the capacitation pattern was evaluated using the CTC assay, as described in the Materials and Methods . The inhibitors used were okadaic acid (black squares), deltamethrin (green diamonds), and endothall (blue triangles). Results were obtained from five different donors and were expressed as the mean±SEM of the percentage of B pattern. The arrow indicates the addition of BSA and bicarbonate. * p

    Article Snippet: The serine-threonine phosphatase inhibitors okadaic acid, deltamethrin, and calmodulin were obtained from Calbiochem Corporation (La Jolla, CA).

    Techniques: Incubation

    Effect of phosphatase inhibitors on phosphorylation of threonine residues of human sperm proteins. Sperm were selected in NCM and then re-suspended in RCM in the absence (RCM) or in the presence of 10 nM okadaic acid (OA), 90 nM endothall (E), or 0.1 nM deltamethrin (D), for 1 min. Then, the samples were processed for western blot analysis with a monoclonal anti-p-Thr antibody. Molecular mass of selected bands is indicated at the right. The lower panels indicate load control with anti β-tubulin antibody. The immunoblots shown are representative of three different experiments with three different donors.

    Journal: PLoS ONE

    Article Title: Protein Phosphatases Decrease Their Activity during Capacitation: A New Requirement for This Event

    doi: 10.1371/journal.pone.0081286

    Figure Lengend Snippet: Effect of phosphatase inhibitors on phosphorylation of threonine residues of human sperm proteins. Sperm were selected in NCM and then re-suspended in RCM in the absence (RCM) or in the presence of 10 nM okadaic acid (OA), 90 nM endothall (E), or 0.1 nM deltamethrin (D), for 1 min. Then, the samples were processed for western blot analysis with a monoclonal anti-p-Thr antibody. Molecular mass of selected bands is indicated at the right. The lower panels indicate load control with anti β-tubulin antibody. The immunoblots shown are representative of three different experiments with three different donors.

    Article Snippet: The serine-threonine phosphatase inhibitors okadaic acid, deltamethrin, and calmodulin were obtained from Calbiochem Corporation (La Jolla, CA).

    Techniques: Western Blot

    Comparison of effector-binding activity of GTP-bound wild-type, G12D, and G12V K-Ras4B 1–188 by use of Raf-1 RBD-binding assay in the absence ( A ) and presence ( B ) of membrane fractions prepared from NIH 3T3 fibroblasts.

    Journal: The FASEB Journal

    Article Title: The higher level of complexity of K-Ras4B activation at the membrane

    doi: 10.1096/fj.15-279091

    Figure Lengend Snippet: Comparison of effector-binding activity of GTP-bound wild-type, G12D, and G12V K-Ras4B 1–188 by use of Raf-1 RBD-binding assay in the absence ( A ) and presence ( B ) of membrane fractions prepared from NIH 3T3 fibroblasts.

    Article Snippet: The GTP-loaded protein samples were used in the Raf proto-oncogene serine/threonine-protein kinase (Raf-1) Ras-binding domain (RBD) assay, following the manufacturer’s instructions (EMD Millipore, Billerica, MA, USA).

    Techniques: Binding Assay, Activity Assay