l media with kanamycin  (New England Biolabs)


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    Name:
    Monarch Plasmid Miniprep Kit
    Description:
    Monarch Plasmid Miniprep Kit 250 preps
    Catalog Number:
    T1010L
    Price:
    354
    Category:
    Plasmid Purification Kits
    Size:
    250 preps
    Buy from Supplier


    Structured Review

    New England Biolabs l media with kanamycin
    Monarch Plasmid Miniprep Kit
    Monarch Plasmid Miniprep Kit 250 preps
    https://www.bioz.com/result/l media with kanamycin/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l media with kanamycin - by Bioz Stars, 2021-09
    96/100 stars

    Images

    1) Product Images from "Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant"

    Article Title: Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-89029-2

    CaldoCas9 based genome editing of T. thermophilus at 65 °C. ( A ) pTTCC_crtI transformants streaked out on fresh media and grown overnight to reveal pigmentation difference in the transformants and WT cells. ( B ) Agarose gel electrophoresis of products from colony PCR amplification of the genomic locus containing crtI in T. thermophilus pTTCC_crtI transformants. Expected band size of the KO allele was 1351 bp and of WT allele 2891 bp. ( C ) pTTCC_purA transformants streaked out on minimal media with and without adenine supplementation (+ Ade − Ade, respectively) to reveal the adenine auxotrophic phenotype. ( D ) Agarose gel electrophoresis of products from colony PCR amplification of genomic locus containing purA in T. thermophilus pTTCC_purA transformants. Expected band size of the KO allele was 1094 bp and the WT allele 2320 bp. ( E ) After growth of pTTCC_purA and pTTCC_crtI transformants in media without antibiotic selection, individual colonies were streaked out on media with and without kanamycin (+ Kan, − Kan, respectively) to reveal the presence or absence of the plasmid. The agarose gel images ( B , D ) have been digitally manipulated for clarity. The original images are provided in supplementary file 5 .
    Figure Legend Snippet: CaldoCas9 based genome editing of T. thermophilus at 65 °C. ( A ) pTTCC_crtI transformants streaked out on fresh media and grown overnight to reveal pigmentation difference in the transformants and WT cells. ( B ) Agarose gel electrophoresis of products from colony PCR amplification of the genomic locus containing crtI in T. thermophilus pTTCC_crtI transformants. Expected band size of the KO allele was 1351 bp and of WT allele 2891 bp. ( C ) pTTCC_purA transformants streaked out on minimal media with and without adenine supplementation (+ Ade − Ade, respectively) to reveal the adenine auxotrophic phenotype. ( D ) Agarose gel electrophoresis of products from colony PCR amplification of genomic locus containing purA in T. thermophilus pTTCC_purA transformants. Expected band size of the KO allele was 1094 bp and the WT allele 2320 bp. ( E ) After growth of pTTCC_purA and pTTCC_crtI transformants in media without antibiotic selection, individual colonies were streaked out on media with and without kanamycin (+ Kan, − Kan, respectively) to reveal the presence or absence of the plasmid. The agarose gel images ( B , D ) have been digitally manipulated for clarity. The original images are provided in supplementary file 5 .

    Techniques Used: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Selection, Plasmid Preparation

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    Article Snippet: .. The Wizard genomic DNA purification kit (Promega, Madison, WI) was used for isolation of bacterial genomic DNA (gDNA), and the Monarch plasmid miniprep kit (New England BioLabs, Ipswich, MA) used for plasmid purification. ..

    Isolation:

    Article Title: c-di-AMP is essential for the virulence of Enterococcus faecalis
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    Article Title: Efficient Secretion and Recombinant Production of a Lactobacillal α-amylase in Lactiplantibacillus plantarum WCFS1: Analysis and Comparison of the Secretion Using Different Signal Peptides
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    Article Title: The method of choice to knock-in large inserts via CRISPR
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    Plasmid Preparation:

    Article Title: c-di-AMP is essential for the virulence of Enterococcus faecalis
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    Article Title: Bacterial type 1A topoisomerases maintain the stability of the genome by preventing and dealing with R-loop-and nucleotide excision repair-dependent topological stress
    Article Snippet: .. Plasmid extraction for supercoiling analysis pACYC184Δtet 5’ DNA extraction for supercoiling analysis was performed by using the Monarch®Plasmid Miniprep Kit (NEB). ..

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    Article Title: Split NanoLuc technology allows quantitation of interactions between PII protein and its receptors with unprecedented sensitivity and reveals transient interactions
    Article Snippet: .. Plasmid DNA was purified from cells using the Monarch Plasmid Miniprep Kit (New England Biolabs, Frankfurt a.M., Germany) according to the manufacturer's instructions. ..

    Article Title: The method of choice to knock-in large inserts via CRISPR
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    Article Title: Genetic engineering biofilms in situ using ultrasound‐mediated DNA delivery
    Article Snippet: .. Colonies formed on the agar plates were resuspended in sterile 0.9% NaCl solution and underwent plasmid extraction procedure using Monarch® Plasmid Miniprep Kit (New England Biolabs, Ipswich, MA, USA) according to manufacturer’s instructions. ..

    Purification:

    Article Title: c-di-AMP is essential for the virulence of Enterococcus faecalis
    Article Snippet: .. The Wizard genomic DNA purification kit (Promega, Madison, WI) was used for isolation of bacterial genomic DNA (gDNA), and the Monarch plasmid miniprep kit (New England BioLabs, Ipswich, MA) used for plasmid purification. ..

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    Article Snippet: .. Plasmid DNA was purified using Monarch® Plasmid Miniprep Kit (NEB) after pre-treatment of S. aureus cells with 20 µg of lysostaphin (Sigma-Aldrich) for 30 min at 37 °C. ..

    Article Title: Split NanoLuc technology allows quantitation of interactions between PII protein and its receptors with unprecedented sensitivity and reveals transient interactions
    Article Snippet: .. Plasmid DNA was purified from cells using the Monarch Plasmid Miniprep Kit (New England Biolabs, Frankfurt a.M., Germany) according to the manufacturer's instructions. ..

    Transformation Assay:

    Article Title: Efficient Secretion and Recombinant Production of a Lactobacillal α-amylase in Lactiplantibacillus plantarum WCFS1: Analysis and Comparison of the Secretion Using Different Signal Peptides
    Article Snippet: .. DNA Manipulation and Transformation Plasmids were isolated from E. coli NEB5α using the Monarch plasmid miniprep kit (New England Biolabs). ..

    Article Title: The method of choice to knock-in large inserts via CRISPR
    Article Snippet: .. Subsequently, the constructs were transformed into bacteria, followed by plasmid isolation (NEB, Monarch Plasmid Miniprep Kit). ..

    DNA Extraction:

    Article Title: Bacterial type 1A topoisomerases maintain the stability of the genome by preventing and dealing with R-loop-and nucleotide excision repair-dependent topological stress
    Article Snippet: .. Plasmid extraction for supercoiling analysis pACYC184Δtet 5’ DNA extraction for supercoiling analysis was performed by using the Monarch®Plasmid Miniprep Kit (NEB). ..

    Construct:

    Article Title: The method of choice to knock-in large inserts via CRISPR
    Article Snippet: .. Subsequently, the constructs were transformed into bacteria, followed by plasmid isolation (NEB, Monarch Plasmid Miniprep Kit). ..

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    New England Biolabs l media with kanamycin
    CaldoCas9 based genome editing of T. thermophilus at 65 °C. ( A ) pTTCC_crtI transformants streaked out on fresh <t>media</t> and grown overnight to reveal pigmentation difference in the transformants and WT cells. ( B ) Agarose gel electrophoresis of products from colony PCR amplification of the genomic locus containing crtI in T. thermophilus pTTCC_crtI transformants. Expected band size of the KO allele was 1351 bp and of WT allele 2891 bp. ( C ) pTTCC_purA transformants streaked out on minimal media <t>with</t> and without adenine supplementation (+ Ade − Ade, respectively) to reveal the adenine auxotrophic phenotype. ( D ) Agarose gel electrophoresis of products from colony PCR amplification of genomic locus containing purA in T. thermophilus pTTCC_purA transformants. Expected band size of the KO allele was 1094 bp and the WT allele 2320 bp. ( E ) After growth of pTTCC_purA and pTTCC_crtI transformants in media without antibiotic selection, individual colonies were streaked out on media with and without <t>kanamycin</t> (+ Kan, − Kan, respectively) to reveal the presence or absence of the plasmid. The agarose gel images ( B , D ) have been digitally manipulated for clarity. The original images are provided in supplementary file 5 .
    L Media With Kanamycin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l media with kanamycin/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l media with kanamycin - by Bioz Stars, 2021-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    CaldoCas9 based genome editing of T. thermophilus at 65 °C. ( A ) pTTCC_crtI transformants streaked out on fresh media and grown overnight to reveal pigmentation difference in the transformants and WT cells. ( B ) Agarose gel electrophoresis of products from colony PCR amplification of the genomic locus containing crtI in T. thermophilus pTTCC_crtI transformants. Expected band size of the KO allele was 1351 bp and of WT allele 2891 bp. ( C ) pTTCC_purA transformants streaked out on minimal media with and without adenine supplementation (+ Ade − Ade, respectively) to reveal the adenine auxotrophic phenotype. ( D ) Agarose gel electrophoresis of products from colony PCR amplification of genomic locus containing purA in T. thermophilus pTTCC_purA transformants. Expected band size of the KO allele was 1094 bp and the WT allele 2320 bp. ( E ) After growth of pTTCC_purA and pTTCC_crtI transformants in media without antibiotic selection, individual colonies were streaked out on media with and without kanamycin (+ Kan, − Kan, respectively) to reveal the presence or absence of the plasmid. The agarose gel images ( B , D ) have been digitally manipulated for clarity. The original images are provided in supplementary file 5 .

    Journal: Scientific Reports

    Article Title: Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variant

    doi: 10.1038/s41598-021-89029-2

    Figure Lengend Snippet: CaldoCas9 based genome editing of T. thermophilus at 65 °C. ( A ) pTTCC_crtI transformants streaked out on fresh media and grown overnight to reveal pigmentation difference in the transformants and WT cells. ( B ) Agarose gel electrophoresis of products from colony PCR amplification of the genomic locus containing crtI in T. thermophilus pTTCC_crtI transformants. Expected band size of the KO allele was 1351 bp and of WT allele 2891 bp. ( C ) pTTCC_purA transformants streaked out on minimal media with and without adenine supplementation (+ Ade − Ade, respectively) to reveal the adenine auxotrophic phenotype. ( D ) Agarose gel electrophoresis of products from colony PCR amplification of genomic locus containing purA in T. thermophilus pTTCC_purA transformants. Expected band size of the KO allele was 1094 bp and the WT allele 2320 bp. ( E ) After growth of pTTCC_purA and pTTCC_crtI transformants in media without antibiotic selection, individual colonies were streaked out on media with and without kanamycin (+ Kan, − Kan, respectively) to reveal the presence or absence of the plasmid. The agarose gel images ( B , D ) have been digitally manipulated for clarity. The original images are provided in supplementary file 5 .

    Article Snippet: A few clones were cultured overnight in liquid L media with kanamycin (30 µg/ml) and plasmids miniprepped (NEB, T1010).

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Selection, Plasmid Preparation