l lysine dihydrochloride  (Millipore)


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    Name:
    L Lysine dihydrochloride
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    Catalog Number:
    l5751
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    Millipore l lysine dihydrochloride
    L Lysine dihydrochloride

    https://www.bioz.com/result/l lysine dihydrochloride/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l lysine dihydrochloride - by Bioz Stars, 2020-09
    88/100 stars

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    Staining:

    Article Title: iTRAQ-based analysis of progerin expression reveals mitochondrial dysfunction, reactive oxygen species accumulation and altered proteostasis
    Article Snippet: .. For staining, the culture plates were fixed in 10 mM sodium periodate, 2 % paraformaldehyde, 75 mM l -lysine dihydrochloride and 37.5 mM dibasic sodium phosphate (Sigma-Aldrich, St. Louis, MO, USA) at pH 7.4 for 15 minutes at room temperature, then air dried and treated with a filtered solution of 0.3 % Oil red O to stain lipid droplets. .. Protein extraction and preparation procedures Cells monolayers were grown until a confluence of 70 % in 100 mm plates, and then washed three times with PBS and harvested with a scraper in SDS lysis buffer (20 % glycerol, 500 mM Tris–HCl, pH 6.8 and 10 % sodium dodecyl sulphate).

    other:

    Article Title: Extracellular L-arginine is required for optimal NO synthesis by eNOS and iNOS in the rat mesenteric artery wall
    Article Snippet: L -arginine hydrochloride, D -arginine hydrochloride, carbamylcholine chloride (carbachol), dexamethasone, gramicidin (from Bacillus brevis ), L -lysine dihydrochloride, phenylephrine hydrochloride and SOD (Cu/Zn-containing enzyme from bovine erythrocytes) were obtained from Sigma (Poole, U.K.).

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  • 95
    Millipore γ secretase substrate
    Inhibitory effect of PL on LPS-induced neuronal cell damage and amyloidogenesis. Cresyl violet staining for the observation of neuronal cell damage in the hippocampal CA3 zone was performed on mice brain sections ( a ). Thioflavin S staining for accumulation of Aβ in the hippocampus was performed on mice brain sections ( b ). Immunostaining of Aβ protein in the hippocampus was performed on mice brain sections with specific primary antibody and the biotinylated secondary antibody ( c ). All stains were performed on 20-µm-thick sections of mouse brains ( n = 3). The level of Aβ 1−42 in mouse brains was measured by ELISA ( n = 3) ( d ). The level of <t>β-secretase</t> activity in mouse brains was measured by β-secretase activity kit ( n = 5) ( e ). The level of <t>γ-secretase</t> activity in mouse brains was measured as described in the “ Materials and Methods ” section ( n = 5) ( f ). Data are expressed as the mean ± S.E.M. ( t test, * p
    γ Secretase Substrate, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/γ secretase substrate/product/Millipore
    Average 95 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    γ secretase substrate - by Bioz Stars, 2020-09
    95/100 stars
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    96
    Millipore mmp specific fluorogenic substrate
    Inhibitory effect of PL on LPS-induced neuronal cell damage and amyloidogenesis. Cresyl violet staining for the observation of neuronal cell damage in the hippocampal CA3 zone was performed on mice brain sections ( a ). Thioflavin S staining for accumulation of Aβ in the hippocampus was performed on mice brain sections ( b ). Immunostaining of Aβ protein in the hippocampus was performed on mice brain sections with specific primary antibody and the biotinylated secondary antibody ( c ). All stains were performed on 20-µm-thick sections of mouse brains ( n = 3). The level of Aβ 1−42 in mouse brains was measured by ELISA ( n = 3) ( d ). The level of <t>β-secretase</t> activity in mouse brains was measured by β-secretase activity kit ( n = 5) ( e ). The level of <t>γ-secretase</t> activity in mouse brains was measured as described in the “ Materials and Methods ” section ( n = 5) ( f ). Data are expressed as the mean ± S.E.M. ( t test, * p
    Mmp Specific Fluorogenic Substrate, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmp specific fluorogenic substrate/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mmp specific fluorogenic substrate - by Bioz Stars, 2020-09
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    85
    Millipore fluorogenic lf substrate cou n nle lys lys lys lys val leu pro ile gln leu asn ala ala thr asp lys qsy 35 gly gly nh2
    Inhibitory effect of PL on LPS-induced neuronal cell damage and amyloidogenesis. Cresyl violet staining for the observation of neuronal cell damage in the hippocampal CA3 zone was performed on mice brain sections ( a ). Thioflavin S staining for accumulation of Aβ in the hippocampus was performed on mice brain sections ( b ). Immunostaining of Aβ protein in the hippocampus was performed on mice brain sections with specific primary antibody and the biotinylated secondary antibody ( c ). All stains were performed on 20-µm-thick sections of mouse brains ( n = 3). The level of Aβ 1−42 in mouse brains was measured by ELISA ( n = 3) ( d ). The level of <t>β-secretase</t> activity in mouse brains was measured by β-secretase activity kit ( n = 5) ( e ). The level of <t>γ-secretase</t> activity in mouse brains was measured as described in the “ Materials and Methods ” section ( n = 5) ( f ). Data are expressed as the mean ± S.E.M. ( t test, * p
    Fluorogenic Lf Substrate Cou N Nle Lys Lys Lys Lys Val Leu Pro Ile Gln Leu Asn Ala Ala Thr Asp Lys Qsy 35 Gly Gly Nh2, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorogenic lf substrate cou n nle lys lys lys lys val leu pro ile gln leu asn ala ala thr asp lys qsy 35 gly gly nh2/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorogenic lf substrate cou n nle lys lys lys lys val leu pro ile gln leu asn ala ala thr asp lys qsy 35 gly gly nh2 - by Bioz Stars, 2020-09
    85/100 stars
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    Inhibitory effect of PL on LPS-induced neuronal cell damage and amyloidogenesis. Cresyl violet staining for the observation of neuronal cell damage in the hippocampal CA3 zone was performed on mice brain sections ( a ). Thioflavin S staining for accumulation of Aβ in the hippocampus was performed on mice brain sections ( b ). Immunostaining of Aβ protein in the hippocampus was performed on mice brain sections with specific primary antibody and the biotinylated secondary antibody ( c ). All stains were performed on 20-µm-thick sections of mouse brains ( n = 3). The level of Aβ 1−42 in mouse brains was measured by ELISA ( n = 3) ( d ). The level of β-secretase activity in mouse brains was measured by β-secretase activity kit ( n = 5) ( e ). The level of γ-secretase activity in mouse brains was measured as described in the “ Materials and Methods ” section ( n = 5) ( f ). Data are expressed as the mean ± S.E.M. ( t test, * p

    Journal: Neuromolecular Medicine

    Article Title: Piperlongumine Improves Lipopolysaccharide-Induced Amyloidogenesis by Suppressing NF-KappaB Pathway

    doi: 10.1007/s12017-018-8495-9

    Figure Lengend Snippet: Inhibitory effect of PL on LPS-induced neuronal cell damage and amyloidogenesis. Cresyl violet staining for the observation of neuronal cell damage in the hippocampal CA3 zone was performed on mice brain sections ( a ). Thioflavin S staining for accumulation of Aβ in the hippocampus was performed on mice brain sections ( b ). Immunostaining of Aβ protein in the hippocampus was performed on mice brain sections with specific primary antibody and the biotinylated secondary antibody ( c ). All stains were performed on 20-µm-thick sections of mouse brains ( n = 3). The level of Aβ 1−42 in mouse brains was measured by ELISA ( n = 3) ( d ). The level of β-secretase activity in mouse brains was measured by β-secretase activity kit ( n = 5) ( e ). The level of γ-secretase activity in mouse brains was measured as described in the “ Materials and Methods ” section ( n = 5) ( f ). Data are expressed as the mean ± S.E.M. ( t test, * p

    Article Snippet: Assay of γ-secretase Activities Solubilized membranes were incubated in 100 µL of assay buffer containing 50 mM Tris–HCl, pH 6.8, 2 mM EDTA, 0.25% CHAPSO (w/v) with 8 µM γ-secretase substrate (Fluorogenic, NMA–Gly–Gly–Val–Val–Ile–Ala–Thr–Val–Lys(DNP)–d -Arg–d -Arg–d -Arg–NH2 , CALBIOCHEM, San Diego, CA, USA) at 37 °C overnight.

    Techniques: Staining, Mouse Assay, Immunostaining, Enzyme-linked Immunosorbent Assay, Activity Assay