Journal: BioMed Research International

Article Title: Highlighting the Potency of Biosurfactants Produced by Pseudomonas Strains as Anti- Legionella Agents

doi: 10.1155/2018/8194368

Figure Lengend Snippet: Antibacterial spectrum of lipopeptide mixtures produced by Pseudomonas strains.

Article Snippet: L. longbeachae ATCC 33484 , 0.4 , 12.5 , 3.3 , 7.9 , 13.1.

Techniques: Produced

Journal: BioMed Research International

Article Title: Highlighting the Potency of Biosurfactants Produced by Pseudomonas Strains as Anti- Legionella Agents

doi: 10.1155/2018/8194368

Figure Lengend Snippet: Antibacterial spectrum of rhamnolipid mixtures produced by Pseudomonas strains.

Article Snippet: L. longbeachae ATCC 33484 , 0.4 , 12.5 , 3.3 , 7.9 , 13.1.

Techniques: Produced

Journal: Scientific Reports

Article Title: Extensive epigenetic modification with large-scale chromosomal and plasmid recombination characterise the Legionella longbeachae serogroup 1 genome

doi: 10.1038/s41598-022-09721-9

Figure Lengend Snippet: Maximum clade credibility tree of 54 L. longbeachae clinical isolates. The scale bar represents the length of 100 years. Isolates are coloured by date of collection (squares), region (circles) and plasmid read coverage (heat map). The years in parentheses represent the estimated timing of coalescent events (95% Highest Posterior Density interval).

Article Snippet: Two more complete genomes have also been obtained, including for the ATCC type-strain from one of the first reported L. longbeachae LD cases in Long Beach, California in 1980 (GenBank: FDAARGOS_201 ) and one we sequenced as part of this study from a NZ patient hospitalised with LD in 2014 (F1157CHC; GenBank NZ_CP020894 ).

Techniques: Plasmid Preparation

Journal: Scientific Reports

Article Title: Extensive epigenetic modification with large-scale chromosomal and plasmid recombination characterise the Legionella longbeachae serogroup 1 genome

doi: 10.1038/s41598-022-09721-9

Figure Lengend Snippet: Circos plot of NZ L. longbeachae isolate F1157CHC. Tracks from the outside to the inside and the predicted gene functional COG category colours are denoted in the key. All data for the SNPs, methylation patterns and GC percentage are values calculated in non-overlapping 1 kb bins. The data for the SNPs and methylation patterns are shown in a log10 scale, and the histograms are also coloured so that larger values are in darker colours. In the centre of the plot are the results from the Reputer analysis, with palindromic repeats in red and forward repeats in blue. The repeats are darker in colour with a smaller Hamming distance between the repeats.

Article Snippet: Two more complete genomes have also been obtained, including for the ATCC type-strain from one of the first reported L. longbeachae LD cases in Long Beach, California in 1980 (GenBank: FDAARGOS_201 ) and one we sequenced as part of this study from a NZ patient hospitalised with LD in 2014 (F1157CHC; GenBank NZ_CP020894 ).

Techniques: Functional Assay, Methylation

Journal: Scientific Reports

Article Title: Extensive epigenetic modification with large-scale chromosomal and plasmid recombination characterise the Legionella longbeachae serogroup 1 genome

doi: 10.1038/s41598-022-09721-9

Figure Lengend Snippet: Mauve alignment of the three complete L. longbeachae sg1 genomes, F1157CHC, FDAARGOS and NSW150 from top to bottom. The 4 main collinear blocks are indicated by colours (LCB1 is red, LCB2 is yellow, LCB3 is green and LCB4 is blue). The sizes of the blocks for LCB1, LCB2, LCB3 and LCB4 are ~ 81 kb, ~ 2265 kb, ~ 1807 kb and ~ 272 kb respectively. Lines between LCBs in the isolates are for the same LCB. In the visualisation the areas within the LCBs without colour indicates underlying differences in the LCB, as explained by the fact that the LCBs are different sizes in each isolate.

Article Snippet: Two more complete genomes have also been obtained, including for the ATCC type-strain from one of the first reported L. longbeachae LD cases in Long Beach, California in 1980 (GenBank: FDAARGOS_201 ) and one we sequenced as part of this study from a NZ patient hospitalised with LD in 2014 (F1157CHC; GenBank NZ_CP020894 ).

Techniques:

Journal: Scientific Reports

Article Title: Extensive epigenetic modification with large-scale chromosomal and plasmid recombination characterise the Legionella longbeachae serogroup 1 genome

doi: 10.1038/s41598-022-09721-9

Figure Lengend Snippet: Mauve alignment of five L. longbeachae reference plasmids.

Article Snippet: Two more complete genomes have also been obtained, including for the ATCC type-strain from one of the first reported L. longbeachae LD cases in Long Beach, California in 1980 (GenBank: FDAARGOS_201 ) and one we sequenced as part of this study from a NZ patient hospitalised with LD in 2014 (F1157CHC; GenBank NZ_CP020894 ).

Techniques:

Journal: Scientific Reports

Article Title: Extensive epigenetic modification with large-scale chromosomal and plasmid recombination characterise the Legionella longbeachae serogroup 1 genome

doi: 10.1038/s41598-022-09721-9

Figure Lengend Snippet: The type I-F CRISPR-Cas element found in some of the L. longbeachae isolates sequenced in this study.

Article Snippet: Two more complete genomes have also been obtained, including for the ATCC type-strain from one of the first reported L. longbeachae LD cases in Long Beach, California in 1980 (GenBank: FDAARGOS_201 ) and one we sequenced as part of this study from a NZ patient hospitalised with LD in 2014 (F1157CHC; GenBank NZ_CP020894 ).

Techniques: CRISPR

Journal: Scientific Reports

Article Title: Extensive epigenetic modification with large-scale chromosomal and plasmid recombination characterise the Legionella longbeachae serogroup 1 genome

doi: 10.1038/s41598-022-09721-9

Figure Lengend Snippet: Methyl-distribution and methyl COG. Both methylation marks are approximately evenly distributed across the L. longbeachae genome. ( A ) The frequency with which m 4 C (above in green) and m 6 A (below in blue) modifications were detected is plotted in 1 kb windows, note the difference in y-axes for each sub-plot. The black box represents the region of unusually high m 6 A modifications highlighted in ( B ) and the locations of individual m 6 A modifications are shown as blue circles for the region with an unusually high rate of this mark. The alternating white and grey boxes represent genes in this region, and are labelled with their NCBI locus ID. The gene responsible for the very high rate of m 6 A modification, B0B39_12100, is a tetratricopeptide repeat protein. m 4 C methylation is not strongly associated with any functional class. ( C ) The proportion of ‘C’ nucleotides are with evidence for methylation in coding and intergenic sequences. Error bars represent a 95% confidence interval, calculated using the normal approximation of a binomial distribution. ( D ) Each point represents the proportion of ‘C’ nucleotides in a given gene that show evidence for methylation. The genes are grouped and shaded by the COG category (x-axis). The box plots summarise the distribution of this value across each COG category.

Article Snippet: Two more complete genomes have also been obtained, including for the ATCC type-strain from one of the first reported L. longbeachae LD cases in Long Beach, California in 1980 (GenBank: FDAARGOS_201 ) and one we sequenced as part of this study from a NZ patient hospitalised with LD in 2014 (F1157CHC; GenBank NZ_CP020894 ).

Techniques: Methylation, Modification, Functional Assay