Journal:

Article Title: Comparison of Virulence of Legionella longbeachae Strains in Guinea Pigs and U937 Macrophage-Like Cells

doi: 10.1128/IAI.69.9.5335-5344.2001

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: An L. pneumophila positive control (strain 130b) was also set up in each experiment, as it is well established that this strain can replicate efficiently in U937 cells ( 8 , 11 , 27 , 28 ). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Species Strain ID 50 a Virulence in guinea pigs Type b L. longbeachae serogroup 1 (O) c d ATCC 33462 Long Beach 4 4.55 ± 0.13 Avirulent 3 L. longbeachae serogroup 2 c ATCC 33484 4.62 ± 0.27 Avirulent 3 5.60 ± 0.28 L. longbeachae serogroup 1 A5H3 2.20 ± 0.25 One of five died 2 2.88 ± 0.28 L. longbeachae serogroup 1 A4C5 1.36 ± 0.10 One of five died 2 2.70 ± 0.42 L. longbeachae serogroup 1 L6C9 3.08 ± 0.14 Two of five died 2 2.85 ± 0.15 L. longbeachae serogroup 1 K5H9 2.53 ± 0.13 Three of five died 2 4.00 ± 0.0 L. pneumophila serogroup 1 130b 2.73 ± 0.13 Virulent 1 3.08 ± 0.28 2.04 ± 0.19 2.38 ± 0.18 3.00 ± 0.28 3.10 ± 0.13 L. longbeachae serogroup 1 A5H5 2.60 ± 0.28 Three of five died 1 3.14 ± 0.10 L. longbeachae serogroup 1 A5E1 3.20 ± 0.25 Three of five died; rapid death 1 3.30 ± 0.0 L. longbeachae serogroup 1 K8B9 2.27 ± 0.30 Four of five died; rapid death 1 2.30 ± 0.0 L. longbeachae serogroup 1 K4A1 3.08 ± 0.30 Three of five died 1 Open in a separate window a The ID 50 is expressed as a log value ± the standard deviation (expressed as a log value).

Techniques:

Journal:

Article Title: Comparison of Virulence of Legionella longbeachae Strains in Guinea Pigs and U937 Macrophage-Like Cells

doi: 10.1128/IAI.69.9.5335-5344.2001

Figure Lengend Snippet: Analysis of virulence of L. longbeachae serogroup 1 strains in a guinea pig model of infection

Article Snippet: An L. pneumophila positive control (strain 130b) was also set up in each experiment, as it is well established that this strain can replicate efficiently in U937 cells ( 8 , 11 , 27 , 28 ). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Species Strain ID 50 a Virulence in guinea pigs Type b L. longbeachae serogroup 1 (O) c d ATCC 33462 Long Beach 4 4.55 ± 0.13 Avirulent 3 L. longbeachae serogroup 2 c ATCC 33484 4.62 ± 0.27 Avirulent 3 5.60 ± 0.28 L. longbeachae serogroup 1 A5H3 2.20 ± 0.25 One of five died 2 2.88 ± 0.28 L. longbeachae serogroup 1 A4C5 1.36 ± 0.10 One of five died 2 2.70 ± 0.42 L. longbeachae serogroup 1 L6C9 3.08 ± 0.14 Two of five died 2 2.85 ± 0.15 L. longbeachae serogroup 1 K5H9 2.53 ± 0.13 Three of five died 2 4.00 ± 0.0 L. pneumophila serogroup 1 130b 2.73 ± 0.13 Virulent 1 3.08 ± 0.28 2.04 ± 0.19 2.38 ± 0.18 3.00 ± 0.28 3.10 ± 0.13 L. longbeachae serogroup 1 A5H5 2.60 ± 0.28 Three of five died 1 3.14 ± 0.10 L. longbeachae serogroup 1 A5E1 3.20 ± 0.25 Three of five died; rapid death 1 3.30 ± 0.0 L. longbeachae serogroup 1 K8B9 2.27 ± 0.30 Four of five died; rapid death 1 2.30 ± 0.0 L. longbeachae serogroup 1 K4A1 3.08 ± 0.30 Three of five died 1 Open in a separate window a The ID 50 is expressed as a log value ± the standard deviation (expressed as a log value).

Techniques:

Journal:

Article Title: Comparison of Virulence of Legionella longbeachae Strains in Guinea Pigs and U937 Macrophage-Like Cells

doi: 10.1128/IAI.69.9.5335-5344.2001

Figure Lengend Snippet: Infectivities of Legionella for U937 cells

Article Snippet: An L. pneumophila positive control (strain 130b) was also set up in each experiment, as it is well established that this strain can replicate efficiently in U937 cells ( 8 , 11 , 27 , 28 ). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Species Strain ID 50 a Virulence in guinea pigs Type b L. longbeachae serogroup 1 (O) c d ATCC 33462 Long Beach 4 4.55 ± 0.13 Avirulent 3 L. longbeachae serogroup 2 c ATCC 33484 4.62 ± 0.27 Avirulent 3 5.60 ± 0.28 L. longbeachae serogroup 1 A5H3 2.20 ± 0.25 One of five died 2 2.88 ± 0.28 L. longbeachae serogroup 1 A4C5 1.36 ± 0.10 One of five died 2 2.70 ± 0.42 L. longbeachae serogroup 1 L6C9 3.08 ± 0.14 Two of five died 2 2.85 ± 0.15 L. longbeachae serogroup 1 K5H9 2.53 ± 0.13 Three of five died 2 4.00 ± 0.0 L. pneumophila serogroup 1 130b 2.73 ± 0.13 Virulent 1 3.08 ± 0.28 2.04 ± 0.19 2.38 ± 0.18 3.00 ± 0.28 3.10 ± 0.13 L. longbeachae serogroup 1 A5H5 2.60 ± 0.28 Three of five died 1 3.14 ± 0.10 L. longbeachae serogroup 1 A5E1 3.20 ± 0.25 Three of five died; rapid death 1 3.30 ± 0.0 L. longbeachae serogroup 1 K8B9 2.27 ± 0.30 Four of five died; rapid death 1 2.30 ± 0.0 L. longbeachae serogroup 1 K4A1 3.08 ± 0.30 Three of five died 1 Open in a separate window a The ID 50 is expressed as a log value ± the standard deviation (expressed as a log value).

Techniques:

Journal:

Article Title: Comparison of Virulence of Legionella longbeachae Strains in Guinea Pigs and U937 Macrophage-Like Cells

doi: 10.1128/IAI.69.9.5335-5344.2001

Figure Lengend Snippet: Electron micrograph showing the intracellular location of L. longbeachae serogroup 1 strains. Lungs were taken from a guinea pig infected with strains ATCC 33462 and A5H5, 3 days after exposure. (A) L. longbeachae serogroup 1 strain A5H5 bacteria (B) observed in a membrane bound vacuole within a macrophage. Potential ribosome studding of the vacuole membrane is shown by an arrow. Host organelles such as mitochondria (M) appear associated with the vacuole. Magnification, ×9,540; bar, 1 μm. (B) Ultrathin sections of a vacuole containing several A5H5 bacteria. Magnification, ×6,360; bar, 1 μm. (C) Macrophage with four membrane vacuoles, each containing one L. longbeachae serogroup 1 ATCC 33462 bacterium. Magnification, ×23,850; bar, 0.5 μm. The arrow indicates the macrophage membrane. (D) Phagosome within a macrophage containing several ATCC 33462 bacteria. The membrane of the vacuole appears smooth walled. Magnification, ×19,875; bar, 0.5 μm. The arrow indicates the phagosome membrane.

Article Snippet: An L. pneumophila positive control (strain 130b) was also set up in each experiment, as it is well established that this strain can replicate efficiently in U937 cells ( 8 , 11 , 27 , 28 ). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Species Strain ID 50 a Virulence in guinea pigs Type b L. longbeachae serogroup 1 (O) c d ATCC 33462 Long Beach 4 4.55 ± 0.13 Avirulent 3 L. longbeachae serogroup 2 c ATCC 33484 4.62 ± 0.27 Avirulent 3 5.60 ± 0.28 L. longbeachae serogroup 1 A5H3 2.20 ± 0.25 One of five died 2 2.88 ± 0.28 L. longbeachae serogroup 1 A4C5 1.36 ± 0.10 One of five died 2 2.70 ± 0.42 L. longbeachae serogroup 1 L6C9 3.08 ± 0.14 Two of five died 2 2.85 ± 0.15 L. longbeachae serogroup 1 K5H9 2.53 ± 0.13 Three of five died 2 4.00 ± 0.0 L. pneumophila serogroup 1 130b 2.73 ± 0.13 Virulent 1 3.08 ± 0.28 2.04 ± 0.19 2.38 ± 0.18 3.00 ± 0.28 3.10 ± 0.13 L. longbeachae serogroup 1 A5H5 2.60 ± 0.28 Three of five died 1 3.14 ± 0.10 L. longbeachae serogroup 1 A5E1 3.20 ± 0.25 Three of five died; rapid death 1 3.30 ± 0.0 L. longbeachae serogroup 1 K8B9 2.27 ± 0.30 Four of five died; rapid death 1 2.30 ± 0.0 L. longbeachae serogroup 1 K4A1 3.08 ± 0.30 Three of five died 1 Open in a separate window a The ID 50 is expressed as a log value ± the standard deviation (expressed as a log value).

Techniques: Infection