Structured Review

PAA Laboratories l glutamine
L Glutamine, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/l glutamine/product/PAA Laboratories
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
l glutamine - by Bioz Stars, 2021-03
86/100 stars

Images

Related Articles

Modification:

Article Title: Identification of a common reference gene pair for qPCR in human mesenchymal stromal cells from different tissue sources treated with VEGF
Article Snippet: Primary cell cultures of MNCs were established by seeding 2 × 107 cells/T75-flask (Nunc, Thermo Scientific, Denmark, cat.no. .. 156494) in complete medium containing Dulbecco’s Modified Eagle Medium, low glucose (1 g/l) (DMEM) supplemented with 25 mM HEPES and L-Glutamin, (PAA Laboratories, Austria, cat.no. .. E15-808), 10% Fetal Bovine Serum Farma grade (FBS, PAA Laboratories, cat.no.

Chloramphenicol Acetyltransferase Assay:

Article Title: Identification of a common reference gene pair for qPCR in human mesenchymal stromal cells from different tissue sources treated with VEGF
Article Snippet: Primary cell cultures of MNCs were established by seeding 2 × 107 cells/T75-flask (Nunc, Thermo Scientific, Denmark, cat.no. .. 156494) in complete medium containing Dulbecco’s Modified Eagle Medium, low glucose (1 g/l) (DMEM) supplemented with 25 mM HEPES and L-Glutamin, (PAA Laboratories, Austria, cat.no. .. E15-808), 10% Fetal Bovine Serum Farma grade (FBS, PAA Laboratories, cat.no.

In Vitro:

Article Title: The blood mMDSC to DC ratio is a sensitive and easy to assess independent predictive factor for epithelial ovarian cancer survival
Article Snippet: Isolation of peripheral blood mononuclear cells PBMC were isolated from venous blood samples by density gradient centrifugation using Ficoll-amidotrizoate (LUMC pharmacy, The Netherlands), cryopreserved in 90% fetal calf serum (FCS; PAA Laboratories, Austria) and 10% DMSO (Sigma-Aldrich, USA), and stored in the vapor phase of liquid nitrogen until further use as described earlier., Handling, storing and staining of the PBMC were done according to the standard operation procedures (SOP) of the department of Medical Oncology at the LUMC by trained personnel. .. Myeloid cell depletion and antigen reactivity testing of PBMC in vitro To test the association between increased circulating myeloid cells and reduced T cell reactivity towards recall and/or tumor antigens, CD14+ monocytes were depleted from two platinum-sensitive EOC patients with high baseline monocyte counts by CD14-guided magnetic cell sorting (Miltenyi Biotec, Germany) as described earlier., CD14-depleted and non-depleted PBMC were subsequently cultured for 11 days with autologous monocytes loaded with either a mix of 30 amino acid long and overlapping influenza virus M1 (FLU) synthetic long peptides (SLP) and memory response mix (MRM) or a mix of 30 amino acid long and overlapping p53 or NY-ESO-1 SLP in IMDM medium (Lonza, Switzerland) supplemented with 10% human AB serum (Capricorn scientific, Germany), 100 U/ml penicillin, 100 μg/ml streptomycin and 2 mM L-glutamin (all from PAA laboratories, Austria) in the presence of 10% T cell Growth Factor (Zeptometrix, USA) and 5 ng/ml IL-7 and IL-15 (Peprotech, USA). .. After 11 days, the cells were tested for antigen reactivity in triplicate wells in a 4-day proliferation assay following re-stimulation with either unloaded (control) or pulsed (FLU/MRM, p53 or NY-ESO-1) monocytes.

FACS:

Article Title: The blood mMDSC to DC ratio is a sensitive and easy to assess independent predictive factor for epithelial ovarian cancer survival
Article Snippet: Isolation of peripheral blood mononuclear cells PBMC were isolated from venous blood samples by density gradient centrifugation using Ficoll-amidotrizoate (LUMC pharmacy, The Netherlands), cryopreserved in 90% fetal calf serum (FCS; PAA Laboratories, Austria) and 10% DMSO (Sigma-Aldrich, USA), and stored in the vapor phase of liquid nitrogen until further use as described earlier., Handling, storing and staining of the PBMC were done according to the standard operation procedures (SOP) of the department of Medical Oncology at the LUMC by trained personnel. .. Myeloid cell depletion and antigen reactivity testing of PBMC in vitro To test the association between increased circulating myeloid cells and reduced T cell reactivity towards recall and/or tumor antigens, CD14+ monocytes were depleted from two platinum-sensitive EOC patients with high baseline monocyte counts by CD14-guided magnetic cell sorting (Miltenyi Biotec, Germany) as described earlier., CD14-depleted and non-depleted PBMC were subsequently cultured for 11 days with autologous monocytes loaded with either a mix of 30 amino acid long and overlapping influenza virus M1 (FLU) synthetic long peptides (SLP) and memory response mix (MRM) or a mix of 30 amino acid long and overlapping p53 or NY-ESO-1 SLP in IMDM medium (Lonza, Switzerland) supplemented with 10% human AB serum (Capricorn scientific, Germany), 100 U/ml penicillin, 100 μg/ml streptomycin and 2 mM L-glutamin (all from PAA laboratories, Austria) in the presence of 10% T cell Growth Factor (Zeptometrix, USA) and 5 ng/ml IL-7 and IL-15 (Peprotech, USA). .. After 11 days, the cells were tested for antigen reactivity in triplicate wells in a 4-day proliferation assay following re-stimulation with either unloaded (control) or pulsed (FLU/MRM, p53 or NY-ESO-1) monocytes.

Article Title: The blood mMDSC to DC ratio is a sensitive and easy to assess independent predictive factor for epithelial ovarian cancer survival
Article Snippet: PBMC were isolated from venous blood samples by density gradient centrifugation using Ficoll-amidotrizoate (LUMC pharmacy, The Netherlands), cryopreserved in 90% fetal calf serum (FCS; PAA Laboratories, Austria) and 10% DMSO (Sigma-Aldrich, USA), and stored in the vapor phase of liquid nitrogen until further use as described earlier., Handling, storing and staining of the PBMC were done according to the standard operation procedures (SOP) of the department of Medical Oncology at the LUMC by trained personnel. .. To test the association between increased circulating myeloid cells and reduced T cell reactivity towards recall and/or tumor antigens, CD14+ monocytes were depleted from two platinum-sensitive EOC patients with high baseline monocyte counts by CD14-guided magnetic cell sorting (Miltenyi Biotec, Germany) as described earlier., CD14-depleted and non-depleted PBMC were subsequently cultured for 11 days with autologous monocytes loaded with either a mix of 30 amino acid long and overlapping influenza virus M1 (FLU) synthetic long peptides (SLP) and memory response mix (MRM) or a mix of 30 amino acid long and overlapping p53 or NY-ESO-1 SLP in IMDM medium (Lonza, Switzerland) supplemented with 10% human AB serum (Capricorn scientific, Germany), 100 U/ml penicillin, 100 μg/ml streptomycin and 2 mM L-glutamin (all from PAA laboratories, Austria) in the presence of 10% T cell Growth Factor (Zeptometrix, USA) and 5 ng/ml IL-7 and IL-15 (Peprotech, USA). .. After 11 days, the cells were tested for antigen reactivity in triplicate wells in a 4-day proliferation assay following re-stimulation with either unloaded (control) or pulsed (FLU/MRM, p53 or NY-ESO-1) monocytes.

Cell Culture:

Article Title: The blood mMDSC to DC ratio is a sensitive and easy to assess independent predictive factor for epithelial ovarian cancer survival
Article Snippet: Isolation of peripheral blood mononuclear cells PBMC were isolated from venous blood samples by density gradient centrifugation using Ficoll-amidotrizoate (LUMC pharmacy, The Netherlands), cryopreserved in 90% fetal calf serum (FCS; PAA Laboratories, Austria) and 10% DMSO (Sigma-Aldrich, USA), and stored in the vapor phase of liquid nitrogen until further use as described earlier., Handling, storing and staining of the PBMC were done according to the standard operation procedures (SOP) of the department of Medical Oncology at the LUMC by trained personnel. .. Myeloid cell depletion and antigen reactivity testing of PBMC in vitro To test the association between increased circulating myeloid cells and reduced T cell reactivity towards recall and/or tumor antigens, CD14+ monocytes were depleted from two platinum-sensitive EOC patients with high baseline monocyte counts by CD14-guided magnetic cell sorting (Miltenyi Biotec, Germany) as described earlier., CD14-depleted and non-depleted PBMC were subsequently cultured for 11 days with autologous monocytes loaded with either a mix of 30 amino acid long and overlapping influenza virus M1 (FLU) synthetic long peptides (SLP) and memory response mix (MRM) or a mix of 30 amino acid long and overlapping p53 or NY-ESO-1 SLP in IMDM medium (Lonza, Switzerland) supplemented with 10% human AB serum (Capricorn scientific, Germany), 100 U/ml penicillin, 100 μg/ml streptomycin and 2 mM L-glutamin (all from PAA laboratories, Austria) in the presence of 10% T cell Growth Factor (Zeptometrix, USA) and 5 ng/ml IL-7 and IL-15 (Peprotech, USA). .. After 11 days, the cells were tested for antigen reactivity in triplicate wells in a 4-day proliferation assay following re-stimulation with either unloaded (control) or pulsed (FLU/MRM, p53 or NY-ESO-1) monocytes.

Article Title: The blood mMDSC to DC ratio is a sensitive and easy to assess independent predictive factor for epithelial ovarian cancer survival
Article Snippet: PBMC were isolated from venous blood samples by density gradient centrifugation using Ficoll-amidotrizoate (LUMC pharmacy, The Netherlands), cryopreserved in 90% fetal calf serum (FCS; PAA Laboratories, Austria) and 10% DMSO (Sigma-Aldrich, USA), and stored in the vapor phase of liquid nitrogen until further use as described earlier., Handling, storing and staining of the PBMC were done according to the standard operation procedures (SOP) of the department of Medical Oncology at the LUMC by trained personnel. .. To test the association between increased circulating myeloid cells and reduced T cell reactivity towards recall and/or tumor antigens, CD14+ monocytes were depleted from two platinum-sensitive EOC patients with high baseline monocyte counts by CD14-guided magnetic cell sorting (Miltenyi Biotec, Germany) as described earlier., CD14-depleted and non-depleted PBMC were subsequently cultured for 11 days with autologous monocytes loaded with either a mix of 30 amino acid long and overlapping influenza virus M1 (FLU) synthetic long peptides (SLP) and memory response mix (MRM) or a mix of 30 amino acid long and overlapping p53 or NY-ESO-1 SLP in IMDM medium (Lonza, Switzerland) supplemented with 10% human AB serum (Capricorn scientific, Germany), 100 U/ml penicillin, 100 μg/ml streptomycin and 2 mM L-glutamin (all from PAA laboratories, Austria) in the presence of 10% T cell Growth Factor (Zeptometrix, USA) and 5 ng/ml IL-7 and IL-15 (Peprotech, USA). .. After 11 days, the cells were tested for antigen reactivity in triplicate wells in a 4-day proliferation assay following re-stimulation with either unloaded (control) or pulsed (FLU/MRM, p53 or NY-ESO-1) monocytes.

Article Title: Molecular mechanism of action and potential biomarkers of growth inhibition of synergistic combination of afatinib and dasatinib against gefitinib-resistant non-small cell lung cancer cells
Article Snippet: As13, As87, and As125 NSCLC cell lines were derived from patients’ pleural effusion by us. .. All cell lines were cultured in RPMI culture medium with Hepes and L-glutamin (PAA laboratories cell Culture Products, Austria), containing 10% Fatal Bovine Serum (Invitrogen, USA), 1% antibiotic with 100 UI/ml Penicillin and 100ug/ml Streptomycin (Invitrogen, USA). .. Incubation condition was set at 37° C in a humidified atmosphere of 95% air and 5% CO2 .

Article Title: CTLA4-Ig immunosuppressive activity at the level of dendritic cell/T cell crosstalk
Article Snippet: .. 2.2 Cell culture medium All assays were performed in IMDM (Invitrogen, Carlsbad, CA, USA) containing 2 mM l -glutamin and 25 mM Hepes supplemented with 10% FBS (PAA Laboratories, Pasching, Austria), antibiotics and 50 μM 2-mercaptoethanol (Sigma, St. Louis, MO, USA), hereafter termed complete medium. ..

Article Title: Features of autophagic cell death in Plasmodium liver-stage parasites
Article Snippet: PbATG8 was amplified from cDNA using primer pair 5′-CC CTCGAG CCATGCCATCATTAAAAG-3′ and 5′-CC GGATCC TTATCCAAAACAACTTTCACAAC-3′ and ligated into pEGFP-C1 with appropriate restriction enzymes (New England Biolabs, R0146, R0136). .. HepG2 cells were purchased from the European Cell Culture Collection and kept in Minimum Essential Medium with Earle’s salts supplemented with 10% FCS, 1% penicillin/streptomycin and 1% L-glutamine (all from PAA Laboratories, E15-024, A15-101, P11-010, M11-004). .. They were cultured at 37 ° C and 5% CO2 and split twice a week using Accutase (PAA Laboratories, L11-007).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    PAA Laboratories l glutamine
    L Glutamine, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l glutamine/product/PAA Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l glutamine - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    PAA Laboratories stable l glutamine
    Stable L Glutamine, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stable l glutamine/product/PAA Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    stable l glutamine - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results