Journal: PLoS ONE

Article Title: Lactobacilli Inactivate Chlamydia trachomatis through Lactic Acid but Not H 2 O 2

doi: 10.1371/journal.pone.0107758

Figure Lengend Snippet: LCM was collected from overnight still cultures of L. crispatus 33197 (Lc33197), L. crispatus 33820 (Lc33820), L. gasseri 33323 (Lg33323) and L. jensenii 25258 (Lj25258). pH values of undiluted (100%) LCM are shown. EBs were treated with undiluted LCM or LCM diluted 10 fold with 0.9% NaCl for 5 min or 1 h. Following treatments, surviving EBs were serially diluted and then inoculated onto McCoy monolayers; resulting inclusion-forming units were scored by fluorescence microscopy. Values were averages ± standard deviations of triplicate experiments.

Article Snippet: L. crispatus strains 33197 (Lc33197) and 33820 (Lc33820), L. gasseri strain 33323 (Lg33323), L. jensenii strain 25258 (Lj25258), and Shigella flexneri 2a strain 2457T ( S. flexneri 2457T) were purchased from ATCC.

Techniques: Fluorescence, Microscopy

Journal: PLoS ONE

Article Title: Lactobacilli Inactivate Chlamydia trachomatis through Lactic Acid but Not H 2 O 2

doi: 10.1371/journal.pone.0107758

Figure Lengend Snippet: Overnight Lactobacillus cultures were diluted with fresh MRS to 0.02 OD 600 . The diluted bacterial suspensions were incubated as still cultures or shaken cultures as described in “ ”. At indicated times, samples were taken for determination of the concentrations of H 2 O 2 (A) and bacteria (B), pH values (C), lactic acid concentrations (D) and anti-chlamydial activities (E). Values were averages ± standard deviations of triplicate experiments. (A-D) Single and double asterisks indicate statistically difference (P<0.05 and P<0.01, respectively) between still cultures and shaken cultures at indicated times. Single and double asterisks in parentheses in B denote statistically significant decline in bacterial concentration following its peak at 20 h (Lc33820 and Lg33323) or 15 h (Lj25258). Double asterisks in E signify statistically significant differences (P<0.01) between indicated two groups. An asterisk in parenthesis indicates that the difference between control MRS and LCM from Lg33323 shaken cultures was deemed statistically insignificant by a two-tailed t test (P<0.1), but significant by a one-tailed t test (P<0.05).

Article Snippet: L. crispatus strains 33197 (Lc33197) and 33820 (Lc33820), L. gasseri strain 33323 (Lg33323), L. jensenii strain 25258 (Lj25258), and Shigella flexneri 2a strain 2457T ( S. flexneri 2457T) were purchased from ATCC.

Techniques: Incubation, Concentration Assay, Two Tailed Test, One-tailed Test

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Carbohydrate utilization profiles of various lactobacilli

Article Snippet: Bioinformatic analysis of the L. gasseri draft genomes revealed that PTS 7, 10 and 15 from L. gasseri ATCC 33323 are not present in all L. gasseri strains whereas the other 12 complete PTS transporters in L. gasseri ATCC 33323 where also found in L. gasseri 202-4, L. gasseri MV-22 and L. gasseri JV-V03.

Techniques:

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Complete and incomplete PTS transporters in selected lactobacilli

Article Snippet: Bioinformatic analysis of the L. gasseri draft genomes revealed that PTS 7, 10 and 15 from L. gasseri ATCC 33323 are not present in all L. gasseri strains whereas the other 12 complete PTS transporters in L. gasseri ATCC 33323 where also found in L. gasseri 202-4, L. gasseri MV-22 and L. gasseri JV-V03.

Techniques:

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Current annotations and predicted substrates of the PTS transporters in L. gasseri ATCC 33323

Article Snippet: Bioinformatic analysis of the L. gasseri draft genomes revealed that PTS 7, 10 and 15 from L. gasseri ATCC 33323 are not present in all L. gasseri strains whereas the other 12 complete PTS transporters in L. gasseri ATCC 33323 where also found in L. gasseri 202-4, L. gasseri MV-22 and L. gasseri JV-V03.

Techniques:

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Presence of complete L. gasseri ATCC 33323 PTS transporters in other L. gasseri strains

Article Snippet: Bioinformatic analysis of the L. gasseri draft genomes revealed that PTS 7, 10 and 15 from L. gasseri ATCC 33323 are not present in all L. gasseri strains whereas the other 12 complete PTS transporters in L. gasseri ATCC 33323 where also found in L. gasseri 202-4, L. gasseri MV-22 and L. gasseri JV-V03.

Techniques:

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Relative fold changes of the complete PTS transporters in L. gasseri ATCC 33323 . Cells grown in semi-synthetic MRS + selected carbohydrate were compared to cells grown in semi-synthetic MRS + fructose. Selected carbohydrates were sucrose (A), cellobiose (B), glucose (C) and mannose (D). RNA was extracted from log phase cells and subjected to two-step real-time PCR. Results are the average of three independent experiments, and error bars indicate standard deviations.

Article Snippet: Bioinformatic analysis of the L. gasseri draft genomes revealed that PTS 7, 10 and 15 from L. gasseri ATCC 33323 are not present in all L. gasseri strains whereas the other 12 complete PTS transporters in L. gasseri ATCC 33323 where also found in L. gasseri 202-4, L. gasseri MV-22 and L. gasseri JV-V03.

Techniques: Real-time Polymerase Chain Reaction

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Growth curves of selected L. gasseri strains . Growth curves of MJM99 (blue), MJM100 (red), MJM101 (green), MJM75 (purple), NCK334 (black) and an uninoculated control (orange) grown in semi-synthetic MRS + selected carbohydrate. Selected carbohydrates were sucrose (A), cellobiose (B), glucose (C) and mannose (D). Results are the average of duplicate wells from one of three independent experiments.

Article Snippet: Bioinformatic analysis of the L. gasseri draft genomes revealed that PTS 7, 10 and 15 from L. gasseri ATCC 33323 are not present in all L. gasseri strains whereas the other 12 complete PTS transporters in L. gasseri ATCC 33323 where also found in L. gasseri 202-4, L. gasseri MV-22 and L. gasseri JV-V03.

Techniques:

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: Bioinformatic analysis of the L. gasseri draft genomes revealed that PTS 7, 10 and 15 from L. gasseri ATCC 33323 are not present in all L. gasseri strains whereas the other 12 complete PTS transporters in L. gasseri ATCC 33323 where also found in L. gasseri 202-4, L. gasseri MV-22 and L. gasseri JV-V03.

Techniques: Plasmid Preparation

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Complete and incomplete PTS transporters in selected lactobacilli

Article Snippet: Additional L. gasseri strains were analyzed for the presence of the fifteen complete PTS transporters found in L. gasseri ATCC 33323 using PCR.

Techniques:

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Current annotations and predicted substrates of the PTS transporters in L. gasseri ATCC 33323

Article Snippet: Additional L. gasseri strains were analyzed for the presence of the fifteen complete PTS transporters found in L. gasseri ATCC 33323 using PCR.

Techniques:

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Presence of complete L. gasseri ATCC 33323 PTS transporters in other L. gasseri strains

Article Snippet: Additional L. gasseri strains were analyzed for the presence of the fifteen complete PTS transporters found in L. gasseri ATCC 33323 using PCR.

Techniques:

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Relative fold changes of the complete PTS transporters in L. gasseri ATCC 33323 . Cells grown in semi-synthetic MRS + selected carbohydrate were compared to cells grown in semi-synthetic MRS + fructose. Selected carbohydrates were sucrose (A), cellobiose (B), glucose (C) and mannose (D). RNA was extracted from log phase cells and subjected to two-step real-time PCR. Results are the average of three independent experiments, and error bars indicate standard deviations.

Article Snippet: Additional L. gasseri strains were analyzed for the presence of the fifteen complete PTS transporters found in L. gasseri ATCC 33323 using PCR.

Techniques: Real-time Polymerase Chain Reaction

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Growth curves of selected L. gasseri strains . Growth curves of MJM99 (blue), MJM100 (red), MJM101 (green), MJM75 (purple), NCK334 (black) and an uninoculated control (orange) grown in semi-synthetic MRS + selected carbohydrate. Selected carbohydrates were sucrose (A), cellobiose (B), glucose (C) and mannose (D). Results are the average of duplicate wells from one of three independent experiments.

Article Snippet: Additional L. gasseri strains were analyzed for the presence of the fifteen complete PTS transporters found in L. gasseri ATCC 33323 using PCR.

Techniques:

Journal: BMC Microbiology

Article Title: The PTS transporters of Lactobacillus gasseri ATCC 33323

doi: 10.1186/1471-2180-10-77

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: Additional L. gasseri strains were analyzed for the presence of the fifteen complete PTS transporters found in L. gasseri ATCC 33323 using PCR.

Techniques: Plasmid Preparation

Journal: Pharmaceuticals

Article Title: Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated Lactobacillus gasseri Confer Non-Identical Cell Protection against Trichomonas vaginalis -Induced Cell Detachment

doi: 10.3390/ph15111350

Figure Lengend Snippet: The prior adhesion of L. gasseri to cells delays Tv G3-induced cell detachment. ( A ) Levels of parasites adhering to HeLa cells with and without prior colonization with L. gasseri ATCC 9857 or KS 120.1 cells. In ( B ), representative differential interference contrast micrographs of cells. In ( C ), progression of cell detachment over time for cells with and without prior colonization with L. gasseri ATCC 9857 or KS 120.1 cells. The micrographs shown are representative of two independent experiments. Each value shown is the mean ± SD of three separate experiments. In ( A , C ), * p < 0.01 for comparisons with Tv G3.

Article Snippet: L. gasseri strain ATCC 9857 inhibits the colonization of vaginal epithelial cells by the genitourinary tract-associated bacterial pathogens Prevotella bivia , Gardnerella vaginosis and Neisseria gonorrhoeae [ , , ], and the T. vaginalis parasite [ , ], through the action of its cells and a cell-surface aggregation-promoting factor [ , ].

Techniques:

Journal: Pharmaceuticals

Article Title: Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated Lactobacillus gasseri Confer Non-Identical Cell Protection against Trichomonas vaginalis -Induced Cell Detachment

doi: 10.3390/ph15111350

Figure Lengend Snippet: The prior adhesion of L. gasseri bacterial cells delays Tv G3-induced alterations to the F-actin network, cell-to-cell contacts, and focal adhesion contacts. In ( A ), representative differential interference contrast micrographs of T. vaginalis -exposed cells precolonized with L. gasseri ATCC 9857 or L. gasseri KS 120.1 cells. In ( B ), percentages of cells displaying rounding and alteration to cell-to-cell contacts (12 h post-infection). In ( C ), confocal laser scanning microscopy (CLSM) images of the F-actin network. In ( D ), decrease in F-actin relative immunofluorescence intensity (RFI) over time. In ( E ), representative CLSM micrographs of vinculin-positive FAs. In ( F ), decrease in vinculin RFI/cell over time. In ( G ), decrease in the number of vinculin-positive FAs/cell over time. The micrographs shown are representative of three independent experiments. Ctl: Control. Each value shown is the mean ± SD of three independent experiments. In ( D , F , G ), * p < 0.01 for comparisons with Tv G3.

Article Snippet: L. gasseri strain ATCC 9857 inhibits the colonization of vaginal epithelial cells by the genitourinary tract-associated bacterial pathogens Prevotella bivia , Gardnerella vaginosis and Neisseria gonorrhoeae [ , , ], and the T. vaginalis parasite [ , ], through the action of its cells and a cell-surface aggregation-promoting factor [ , ].

Techniques: Infection, Confocal Laser Scanning Microscopy, Immunofluorescence

Journal: Molecules

Article Title: Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis

doi: 10.3390/molecules27175557

Figure Lengend Snippet: The percentages of P. mirabilis isolate growth inhibition according to broth microdilution method. The results are presented as the mean ± standard deviation (SD) of three experiments. All the results are statistically significant ( p < 0.05) according to Cochran’s Q test.

Article Snippet: The greatest activity against pathogenic strains, with the percentage range of growth inhibition 62–99%, was observed in nine isolates ( L. crispatus 1.2, 4, 13.2, 40, 41.4, L. saerimneri 3.2, L. gasseri 35.3, and two references strains L.gasseri ATCC 33323 and L.crispatus ATCC 33197), in contrast to the supernatants of L. crispatus 6.2 and L. jensenii 22.2, which showed the lowest percentage of P. mirabilis growth inhibition (62–79%).

Techniques: Inhibition, Standard Deviation

Journal: Molecules

Article Title: Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis

doi: 10.3390/molecules27175557

Figure Lengend Snippet: The concentration (mM) of organic acids: lactic, citric, and succinic acids in cell-free supernatants of Lactobacillus strains. The results are presented as the means of three experiments.

Article Snippet: The greatest activity against pathogenic strains, with the percentage range of growth inhibition 62–99%, was observed in nine isolates ( L. crispatus 1.2, 4, 13.2, 40, 41.4, L. saerimneri 3.2, L. gasseri 35.3, and two references strains L.gasseri ATCC 33323 and L.crispatus ATCC 33197), in contrast to the supernatants of L. crispatus 6.2 and L. jensenii 22.2, which showed the lowest percentage of P. mirabilis growth inhibition (62–79%).

Techniques: Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Isolation of a Highly Thermostable Bile Salt Hydrolase With Broad Substrate Specificity From Lactobacillus paragasseri

doi: 10.3389/fmicb.2022.810872

Figure Lengend Snippet: Thermal resistance assay of L. paragasseri JCM 5343 T . Strain JCM 5343 T culture was heat treated at 85°C for 30–150 min (intervals of 30 min). After heat treatment, the resulting cultures were spotted on de Man-Rogosa-Sharpe medium (MRS) agar plates and incubated at 37°C under anaerobic conditions. Scale bar indicates 1 cm.

Article Snippet: A probiotic lactic acid bacterium L. paragasseri strain JCM 5343 T (= KCTC 3172 T = ATCC 4963 T ) ( ) was purchased from the Japan Collection of Microorganisms (JCM, RIKEN BRC, Ibaraki, Japan).

Techniques: Incubation