l arginine arg0  (Millipore)


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    Structured Review

    Millipore l arginine arg0
    L Arginine Arg0, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l arginine arg0/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l arginine arg0 - by Bioz Stars, 2020-04
    86/100 stars

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    Related Articles

    Modification:

    Article Title: Modulation of cell proteome by 25-hydroxycholesterol and 27-hydroxycholesterol: A link between cholesterol metabolism and antiviral defense.
    Article Snippet: Cell culture and SILAC labelling Human epithelial adenocarcinoma HeLa cells (ATCC® CCL-2™) and African green monkey kidney epithelial cells (MA104) were propagated in Dulbecco's modified Eagle's medium (DMEM) (Gibco-BRL, Gaithersburg, MD) supplemented with heat-inactivated 10% fetal bovine serum (FBS) (Gibco/BRL) and 1% penicillin-streptomycin solution 100X (EuroClone), at 37°C in an atmosphere of 5% of CO 2 . .. To this medium, the appropriate amino acids were added as follows: unlabelled L-lysine (Lys0) and L-arginine (Arg0) (both from Sigma) were used to obtain light-labelled cells, and 13 C 6 15 N 4 L-arginine (Arg10, CNLM-539, Cambridge Isotope Laboratory) and 13 C 6 15 N 2 L-lysine (Lys8, CNLM-291, Cambridge Isotope Laboratory)

    Concentration Assay:

    Article Title: Modulation of cell proteome by 25-hydroxycholesterol and 27-hydroxycholesterol: A link between cholesterol metabolism and antiviral defense.
    Article Snippet: To this medium, the appropriate amino acids were added as follows: unlabelled L-lysine (Lys0) and L-arginine (Arg0) (both from Sigma) were used to obtain light-labelled cells, and 13 C 6 15 N 4 L-arginine (Arg10, CNLM-539, Cambridge Isotope Laboratory) and 13 C 6 15 N 2 L-lysine (Lys8, CNLM-291, Cambridge Isotope Laboratory) .. The final amino acid concentration corresponded to the standard DMEM composition (i.e.84 mg/l arginine and 146 mg/l lysine).

    Article Title: Quantitative Chemical Proteomics Identifies Novel Targets of the Anti-cancer Multi-kinase Inhibitor E-3810 *
    Article Snippet: A2780 cells were SILAC-labeled using RPMI 1640 medium deficient in lysine and arginine (Invitrogen) supplemented with 10% dialyzed fetal bovine serum (Invitrogen) and the appropriate amino acids as follows: unlabeled l -lysine (Lys0) and l -arginine (Arg0) (both from Sigma) were used to obtain light-labeled cells, and 13 C6 15 N4 l -arginine (Arg10, Sigma, 608033) and 13 C6 15 N2 l -lysine (Lys8, Sigma, 608041) were used to obtain heavy-labeled cells. .. The final amino acid concentration corresponded to the standard RPMI 1640 composition (i.e. 200 mg/l arginine and 40 mg/l lysine).

    Cell Culture:

    Article Title: Modulation of cell proteome by 25-hydroxycholesterol and 27-hydroxycholesterol: A link between cholesterol metabolism and antiviral defense.
    Article Snippet: Paragraph title: Cell culture and SILAC labelling ... To this medium, the appropriate amino acids were added as follows: unlabelled L-lysine (Lys0) and L-arginine (Arg0) (both from Sigma) were used to obtain light-labelled cells, and 13 C 6 15 N 4 L-arginine (Arg10, CNLM-539, Cambridge Isotope Laboratory) and 13 C 6 15 N 2 L-lysine (Lys8, CNLM-291, Cambridge Isotope Laboratory)

    Article Title: Quantitative Chemical Proteomics Identifies Novel Targets of the Anti-cancer Multi-kinase Inhibitor E-3810 *
    Article Snippet: Paragraph title: Cell Culture and SILAC Labeling ... A2780 cells were SILAC-labeled using RPMI 1640 medium deficient in lysine and arginine (Invitrogen) supplemented with 10% dialyzed fetal bovine serum (Invitrogen) and the appropriate amino acids as follows: unlabeled l -lysine (Lys0) and l -arginine (Arg0) (both from Sigma) were used to obtain light-labeled cells, and 13 C6 15 N4 l -arginine (Arg10, Sigma, 608033) and 13 C6 15 N2 l -lysine (Lys8, Sigma, 608041) were used to obtain heavy-labeled cells.

    Labeling:

    Article Title: Quantitative Chemical Proteomics Identifies Novel Targets of the Anti-cancer Multi-kinase Inhibitor E-3810 *
    Article Snippet: Paragraph title: Cell Culture and SILAC Labeling ... A2780 cells were SILAC-labeled using RPMI 1640 medium deficient in lysine and arginine (Invitrogen) supplemented with 10% dialyzed fetal bovine serum (Invitrogen) and the appropriate amino acids as follows: unlabeled l -lysine (Lys0) and l -arginine (Arg0) (both from Sigma) were used to obtain light-labeled cells, and 13 C6 15 N4 l -arginine (Arg10, Sigma, 608033) and 13 C6 15 N2 l -lysine (Lys8, Sigma, 608041) were used to obtain heavy-labeled cells.

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    Millipore microcystin lr
    Comparison of <t>microcystin-LR</t> (MC-LR) equivalents determined by HPLC with DAD and PP1 inhibition in extracts of laboratory strains and environmental samples of cyanobacteria. Extracts were analyzed by HPLC with DAD, and the results were compared to the results of PP1 analyses of the same extracts performed in the presence of preimmune (null) serum (■) and microcystin-LR antiserum (●), expressed in micrograms per milligram of cyanobacterial cells. The vertical error bars indicate standard deviations where their dimensions exceed those of the symbols. Regression lines are shown for HPLC-DAD analysis versus PP1 inhibition in the presence of preimmune serum (—) ( y = 1.25 x − 0.02; R 2 = 0.94; P
    Microcystin Lr, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microcystin lr/product/Millipore
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    microcystin lr - by Bioz Stars, 2020-04
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    91
    Millipore pp2a inhibitor microcystin lr
    Tracer coupling supported by Cx79.8 in HeLa cells. Drug treatments in all panels include no treatment (Con), 10 μM PKA antagonist Rp-8-cpt-cAMPS (Rp), 10 μM PKA agonist Sp-8-cpt-cAMPS (Sp), and 0.5 nM <t>PP2A</t> antagonist <t>Microcystin</t> LR (Mic). (A) Transfection with EGFP empty vector alone; (B) transfection with Cx79.8-EGFP; (C) transfection with untagged Cx79.8. Data shown are mean diffusion coefficients for Neurobiotin transfer for 3 to 6 experiments; ** p
    Pp2a Inhibitor Microcystin Lr, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp2a inhibitor microcystin lr/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pp2a inhibitor microcystin lr - by Bioz Stars, 2020-04
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    94
    Millipore z phe arg amc substrate
    Tracer coupling supported by Cx79.8 in HeLa cells. Drug treatments in all panels include no treatment (Con), 10 μM PKA antagonist Rp-8-cpt-cAMPS (Rp), 10 μM PKA agonist Sp-8-cpt-cAMPS (Sp), and 0.5 nM <t>PP2A</t> antagonist <t>Microcystin</t> LR (Mic). (A) Transfection with EGFP empty vector alone; (B) transfection with Cx79.8-EGFP; (C) transfection with untagged Cx79.8. Data shown are mean diffusion coefficients for Neurobiotin transfer for 3 to 6 experiments; ** p
    Z Phe Arg Amc Substrate, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z phe arg amc substrate/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z phe arg amc substrate - by Bioz Stars, 2020-04
    94/100 stars
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    Image Search Results


    Comparison of microcystin-LR (MC-LR) equivalents determined by HPLC with DAD and PP1 inhibition in extracts of laboratory strains and environmental samples of cyanobacteria. Extracts were analyzed by HPLC with DAD, and the results were compared to the results of PP1 analyses of the same extracts performed in the presence of preimmune (null) serum (■) and microcystin-LR antiserum (●), expressed in micrograms per milligram of cyanobacterial cells. The vertical error bars indicate standard deviations where their dimensions exceed those of the symbols. Regression lines are shown for HPLC-DAD analysis versus PP1 inhibition in the presence of preimmune serum (—) ( y = 1.25 x − 0.02; R 2 = 0.94; P

    Journal: Applied and Environmental Microbiology

    Article Title: Colorimetric Immuno-Protein Phosphatase Inhibition Assay for Specific Detection of Microcystins and Nodularins of Cyanobacteria

    doi: 10.1128/AEM.67.2.904-909.2001

    Figure Lengend Snippet: Comparison of microcystin-LR (MC-LR) equivalents determined by HPLC with DAD and PP1 inhibition in extracts of laboratory strains and environmental samples of cyanobacteria. Extracts were analyzed by HPLC with DAD, and the results were compared to the results of PP1 analyses of the same extracts performed in the presence of preimmune (null) serum (■) and microcystin-LR antiserum (●), expressed in micrograms per milligram of cyanobacterial cells. The vertical error bars indicate standard deviations where their dimensions exceed those of the symbols. Regression lines are shown for HPLC-DAD analysis versus PP1 inhibition in the presence of preimmune serum (—) ( y = 1.25 x − 0.02; R 2 = 0.94; P

    Article Snippet: Solutions of purified microcystin-LR (0 to 1,000 μg liter−1 ) were prepared by using Milli-Q (Millipore) water.

    Techniques: High Performance Liquid Chromatography, Inhibition, Environmental Sampling

    Tracer coupling supported by Cx79.8 in HeLa cells. Drug treatments in all panels include no treatment (Con), 10 μM PKA antagonist Rp-8-cpt-cAMPS (Rp), 10 μM PKA agonist Sp-8-cpt-cAMPS (Sp), and 0.5 nM PP2A antagonist Microcystin LR (Mic). (A) Transfection with EGFP empty vector alone; (B) transfection with Cx79.8-EGFP; (C) transfection with untagged Cx79.8. Data shown are mean diffusion coefficients for Neurobiotin transfer for 3 to 6 experiments; ** p

    Journal: Developmental neurobiology

    Article Title: Zebrafish Connexin 79.8 (Gja8a): a lens connexin used as an electrical synapse in some neurons

    doi: 10.1002/dneu.22418

    Figure Lengend Snippet: Tracer coupling supported by Cx79.8 in HeLa cells. Drug treatments in all panels include no treatment (Con), 10 μM PKA antagonist Rp-8-cpt-cAMPS (Rp), 10 μM PKA agonist Sp-8-cpt-cAMPS (Sp), and 0.5 nM PP2A antagonist Microcystin LR (Mic). (A) Transfection with EGFP empty vector alone; (B) transfection with Cx79.8-EGFP; (C) transfection with untagged Cx79.8. Data shown are mean diffusion coefficients for Neurobiotin transfer for 3 to 6 experiments; ** p

    Article Snippet: Transfected HeLa cell coverslips were incubated in oxygenated Ringer's medium at 34°C for 10 min with or without PKA activator Sp-8-cpt-cAMPS (10 μM; Axxora, Farmingdale, NY), PKA inhibitor Rp-8-cpt-cAMPS (10 μM; Axxora), or PP2A inhibitor Microcystin-LR (0.5 nM; EMD Millipore, Billerica, MA).

    Techniques: Cycling Probe Technology, Transfection, Plasmid Preparation, Diffusion-based Assay