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Millipore kv7 2 antibody
Expressions of Kv channels at the AIS in control and deprived neurons. ( a ) Macroscopic view of brainstem auditory nuclei at 7 days after auditory deprivation. Kv1.1 immunosignals were identified in NM and its target nuclei (nucleus laminaris, NL), but the signals were more prominent in control (left) than in deprived side (right) in NM. ( b ) Schematic drawing of avian auditory pathway. ( c – e ) NM neurons stained with Kv (left, red) and Nav pan (middle, green) antibodies were merged (right). Kv1.1 ( c ), Kv3.1 ( d ) and <t>Kv7.2</t> ( e ). Control (upper) and deprived (lower) neurons were from the same slice. ( f ) Intensities of immunosignals at the soma (circles) and the axon in c – e were plotted. The AIS was identified by fibrous Nav signals (arrowheads in c – e ; vertical broken lines in f ). Average Kv intensity at the AIS is indicated by grey horizontal lines. Dot-like Nav signals correspond to nodes of Ranvier 6 (middle). ( g , h ) Relative signal intensities of Nav pan, Kv1.1, Kv3.1 and Kv7.2 in the soma ( g ) and the AIS ( h ) at 7 days after auditory deprivation. Relative length of the AIS ( h , right). Ratios were calculated in each slice and averaged for five animals (see also Supplementary Fig. 1 ). Kv1.1 decreased at both the soma and the AIS, whereas Kv7.2 and Kv3.1 increased only at the AIS. ( i ) Relative mRNA levels at 7 days after auditory deprivation. Three sets of experiments were conducted for individual molecules (Methods). The uniform reductions of mRNA may suggest the involvement of post-transcriptional mechanisms. ( j ) Time course of changes in signal intensity of Kv1.1 (left), Kv3.1 (middle) and Kv7.2 (right). The time course of changes in the AIS length is plotted (left, dots). Four, four, five and six chicks were used for 1, 3, 7 and 14
Kv7 2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kv7 2 antibody/product/Millipore
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
kv7 2 antibody - by Bioz Stars, 2022-08
99/100 stars

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1) Product Images from "Redistribution of Kv1 and Kv7 enhances neuronal excitability during structural axon initial segment plasticity"

Article Title: Redistribution of Kv1 and Kv7 enhances neuronal excitability during structural axon initial segment plasticity

Journal: Nature Communications

doi: 10.1038/ncomms9815

Expressions of Kv channels at the AIS in control and deprived neurons. ( a ) Macroscopic view of brainstem auditory nuclei at 7 days after auditory deprivation. Kv1.1 immunosignals were identified in NM and its target nuclei (nucleus laminaris, NL), but the signals were more prominent in control (left) than in deprived side (right) in NM. ( b ) Schematic drawing of avian auditory pathway. ( c – e ) NM neurons stained with Kv (left, red) and Nav pan (middle, green) antibodies were merged (right). Kv1.1 ( c ), Kv3.1 ( d ) and Kv7.2 ( e ). Control (upper) and deprived (lower) neurons were from the same slice. ( f ) Intensities of immunosignals at the soma (circles) and the axon in c – e were plotted. The AIS was identified by fibrous Nav signals (arrowheads in c – e ; vertical broken lines in f ). Average Kv intensity at the AIS is indicated by grey horizontal lines. Dot-like Nav signals correspond to nodes of Ranvier 6 (middle). ( g , h ) Relative signal intensities of Nav pan, Kv1.1, Kv3.1 and Kv7.2 in the soma ( g ) and the AIS ( h ) at 7 days after auditory deprivation. Relative length of the AIS ( h , right). Ratios were calculated in each slice and averaged for five animals (see also Supplementary Fig. 1 ). Kv1.1 decreased at both the soma and the AIS, whereas Kv7.2 and Kv3.1 increased only at the AIS. ( i ) Relative mRNA levels at 7 days after auditory deprivation. Three sets of experiments were conducted for individual molecules (Methods). The uniform reductions of mRNA may suggest the involvement of post-transcriptional mechanisms. ( j ) Time course of changes in signal intensity of Kv1.1 (left), Kv3.1 (middle) and Kv7.2 (right). The time course of changes in the AIS length is plotted (left, dots). Four, four, five and six chicks were used for 1, 3, 7 and 14
Figure Legend Snippet: Expressions of Kv channels at the AIS in control and deprived neurons. ( a ) Macroscopic view of brainstem auditory nuclei at 7 days after auditory deprivation. Kv1.1 immunosignals were identified in NM and its target nuclei (nucleus laminaris, NL), but the signals were more prominent in control (left) than in deprived side (right) in NM. ( b ) Schematic drawing of avian auditory pathway. ( c – e ) NM neurons stained with Kv (left, red) and Nav pan (middle, green) antibodies were merged (right). Kv1.1 ( c ), Kv3.1 ( d ) and Kv7.2 ( e ). Control (upper) and deprived (lower) neurons were from the same slice. ( f ) Intensities of immunosignals at the soma (circles) and the axon in c – e were plotted. The AIS was identified by fibrous Nav signals (arrowheads in c – e ; vertical broken lines in f ). Average Kv intensity at the AIS is indicated by grey horizontal lines. Dot-like Nav signals correspond to nodes of Ranvier 6 (middle). ( g , h ) Relative signal intensities of Nav pan, Kv1.1, Kv3.1 and Kv7.2 in the soma ( g ) and the AIS ( h ) at 7 days after auditory deprivation. Relative length of the AIS ( h , right). Ratios were calculated in each slice and averaged for five animals (see also Supplementary Fig. 1 ). Kv1.1 decreased at both the soma and the AIS, whereas Kv7.2 and Kv3.1 increased only at the AIS. ( i ) Relative mRNA levels at 7 days after auditory deprivation. Three sets of experiments were conducted for individual molecules (Methods). The uniform reductions of mRNA may suggest the involvement of post-transcriptional mechanisms. ( j ) Time course of changes in signal intensity of Kv1.1 (left), Kv3.1 (middle) and Kv7.2 (right). The time course of changes in the AIS length is plotted (left, dots). Four, four, five and six chicks were used for 1, 3, 7 and 14

Techniques Used: Staining

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    Millipore kv7 2 antibody
    Expressions of Kv channels at the AIS in control and deprived neurons. ( a ) Macroscopic view of brainstem auditory nuclei at 7 days after auditory deprivation. Kv1.1 immunosignals were identified in NM and its target nuclei (nucleus laminaris, NL), but the signals were more prominent in control (left) than in deprived side (right) in NM. ( b ) Schematic drawing of avian auditory pathway. ( c – e ) NM neurons stained with Kv (left, red) and Nav pan (middle, green) antibodies were merged (right). Kv1.1 ( c ), Kv3.1 ( d ) and <t>Kv7.2</t> ( e ). Control (upper) and deprived (lower) neurons were from the same slice. ( f ) Intensities of immunosignals at the soma (circles) and the axon in c – e were plotted. The AIS was identified by fibrous Nav signals (arrowheads in c – e ; vertical broken lines in f ). Average Kv intensity at the AIS is indicated by grey horizontal lines. Dot-like Nav signals correspond to nodes of Ranvier 6 (middle). ( g , h ) Relative signal intensities of Nav pan, Kv1.1, Kv3.1 and Kv7.2 in the soma ( g ) and the AIS ( h ) at 7 days after auditory deprivation. Relative length of the AIS ( h , right). Ratios were calculated in each slice and averaged for five animals (see also Supplementary Fig. 1 ). Kv1.1 decreased at both the soma and the AIS, whereas Kv7.2 and Kv3.1 increased only at the AIS. ( i ) Relative mRNA levels at 7 days after auditory deprivation. Three sets of experiments were conducted for individual molecules (Methods). The uniform reductions of mRNA may suggest the involvement of post-transcriptional mechanisms. ( j ) Time course of changes in signal intensity of Kv1.1 (left), Kv3.1 (middle) and Kv7.2 (right). The time course of changes in the AIS length is plotted (left, dots). Four, four, five and six chicks were used for 1, 3, 7 and 14
    Kv7 2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kv7 2 antibody/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kv7 2 antibody - by Bioz Stars, 2022-08
    99/100 stars
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    Expressions of Kv channels at the AIS in control and deprived neurons. ( a ) Macroscopic view of brainstem auditory nuclei at 7 days after auditory deprivation. Kv1.1 immunosignals were identified in NM and its target nuclei (nucleus laminaris, NL), but the signals were more prominent in control (left) than in deprived side (right) in NM. ( b ) Schematic drawing of avian auditory pathway. ( c – e ) NM neurons stained with Kv (left, red) and Nav pan (middle, green) antibodies were merged (right). Kv1.1 ( c ), Kv3.1 ( d ) and Kv7.2 ( e ). Control (upper) and deprived (lower) neurons were from the same slice. ( f ) Intensities of immunosignals at the soma (circles) and the axon in c – e were plotted. The AIS was identified by fibrous Nav signals (arrowheads in c – e ; vertical broken lines in f ). Average Kv intensity at the AIS is indicated by grey horizontal lines. Dot-like Nav signals correspond to nodes of Ranvier 6 (middle). ( g , h ) Relative signal intensities of Nav pan, Kv1.1, Kv3.1 and Kv7.2 in the soma ( g ) and the AIS ( h ) at 7 days after auditory deprivation. Relative length of the AIS ( h , right). Ratios were calculated in each slice and averaged for five animals (see also Supplementary Fig. 1 ). Kv1.1 decreased at both the soma and the AIS, whereas Kv7.2 and Kv3.1 increased only at the AIS. ( i ) Relative mRNA levels at 7 days after auditory deprivation. Three sets of experiments were conducted for individual molecules (Methods). The uniform reductions of mRNA may suggest the involvement of post-transcriptional mechanisms. ( j ) Time course of changes in signal intensity of Kv1.1 (left), Kv3.1 (middle) and Kv7.2 (right). The time course of changes in the AIS length is plotted (left, dots). Four, four, five and six chicks were used for 1, 3, 7 and 14

    Journal: Nature Communications

    Article Title: Redistribution of Kv1 and Kv7 enhances neuronal excitability during structural axon initial segment plasticity

    doi: 10.1038/ncomms9815

    Figure Lengend Snippet: Expressions of Kv channels at the AIS in control and deprived neurons. ( a ) Macroscopic view of brainstem auditory nuclei at 7 days after auditory deprivation. Kv1.1 immunosignals were identified in NM and its target nuclei (nucleus laminaris, NL), but the signals were more prominent in control (left) than in deprived side (right) in NM. ( b ) Schematic drawing of avian auditory pathway. ( c – e ) NM neurons stained with Kv (left, red) and Nav pan (middle, green) antibodies were merged (right). Kv1.1 ( c ), Kv3.1 ( d ) and Kv7.2 ( e ). Control (upper) and deprived (lower) neurons were from the same slice. ( f ) Intensities of immunosignals at the soma (circles) and the axon in c – e were plotted. The AIS was identified by fibrous Nav signals (arrowheads in c – e ; vertical broken lines in f ). Average Kv intensity at the AIS is indicated by grey horizontal lines. Dot-like Nav signals correspond to nodes of Ranvier 6 (middle). ( g , h ) Relative signal intensities of Nav pan, Kv1.1, Kv3.1 and Kv7.2 in the soma ( g ) and the AIS ( h ) at 7 days after auditory deprivation. Relative length of the AIS ( h , right). Ratios were calculated in each slice and averaged for five animals (see also Supplementary Fig. 1 ). Kv1.1 decreased at both the soma and the AIS, whereas Kv7.2 and Kv3.1 increased only at the AIS. ( i ) Relative mRNA levels at 7 days after auditory deprivation. Three sets of experiments were conducted for individual molecules (Methods). The uniform reductions of mRNA may suggest the involvement of post-transcriptional mechanisms. ( j ) Time course of changes in signal intensity of Kv1.1 (left), Kv3.1 (middle) and Kv7.2 (right). The time course of changes in the AIS length is plotted (left, dots). Four, four, five and six chicks were used for 1, 3, 7 and 14

    Article Snippet: Immunohistochemistry The mouse monoclonal Nav pan antibody (5 μg ml−1 , Sigma), the rabbit polyclonal Kv1.1 antibody (1 μg ml−1 , Alomone), the Kv1.2 antibody (1 μg ml−1 , Alomone), the Kv3.1b antibody (1.5 μg ml−1 , Sigma), the Kv7.2 antibody (1.1 μg ml−1 , Sigma) and the Kv7.3 antibody (1.5 μg ml−1 , Alomone) were used for immunohistochemistry.

    Techniques: Staining