rabbit polyclonal anti kv4 2 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit polyclonal anti kv4 2 antibody
    <t>Kv4.3</t> immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with <t>antibodies</t> directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.
    Rabbit Polyclonal Anti Kv4 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti kv4 2 antibody/product/Alomone Labs
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti kv4 2 antibody - by Bioz Stars, 2022-12
    94/100 stars

    Images

    1) Product Images from "Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve"

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    Journal: Frontiers in Neuroanatomy

    doi: 10.3389/fnana.2022.958986

    Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.
    Figure Legend Snippet: Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.

    Techniques Used:

    Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.
    Figure Legend Snippet: Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.

    Techniques Used: Binding Assay, Microscopy, Western Blot, Fluorescence, Molecular Weight, Migration

    Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.
    Figure Legend Snippet: Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.

    Techniques Used: Immunoprecipitation, Western Blot, Electrophoresis, Molecular Weight, Migration

    2) Product Images from "Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve"

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    Journal: Frontiers in Neuroanatomy

    doi: 10.3389/fnana.2022.958986

    Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.
    Figure Legend Snippet: Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.

    Techniques Used:

    Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.
    Figure Legend Snippet: Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.

    Techniques Used: Binding Assay, Microscopy, Western Blot, Fluorescence, Molecular Weight, Migration

    Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.
    Figure Legend Snippet: Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.

    Techniques Used: Immunoprecipitation, Western Blot, Electrophoresis, Molecular Weight, Migration

    3) Product Images from "Potassium Channel Conductance Is Involved in Phenylephrine-Induced Spontaneous Firing of Serotonergic Neurons in the Dorsal Raphe Nucleus"

    Article Title: Potassium Channel Conductance Is Involved in Phenylephrine-Induced Spontaneous Firing of Serotonergic Neurons in the Dorsal Raphe Nucleus

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2022.891912

    Expression of A-type K + channels and their contribution to the PE-induced spontaneous firing of the DRN 5-HT neurons. (A) Expression of A-type K + channel-related subfamily members assessed using single-cell PCR analysis in the DRN neurons. (i) Representative image of single-cell PCR products showing the presence of different Kv subunits. (ii) Proportion of Kv1.4, 3.3, 3.4, and Kv4s-positive neurons in the TPH-positive neurons ( n = 20). (B) (i) Confocal images of Kv4.2 and 4.3 protein expression in slice of the DRN, assessed using immunofluorescence methods. Scale bar = 50 μm. (ii) Proportion of Kv4.2 and 4.3-positive neurons in the TPH-positive neurons ( n = 200). (C) PE potently inhibited A-type currents recorded using whole-cell patch clamp in the DRN 5-HT neurons. (i) Recording protocol used and the typical current traces recorded; the latter were from –20 mV. The current amplitude at the beginning of the –20 mV step was measured (dotted square, enlarged in inset). (ii) Time course for current amplitudes measured in (i) . (iii) Summarized data for experiments shown in (i,ii) . Paired t -test, ** p
    Figure Legend Snippet: Expression of A-type K + channels and their contribution to the PE-induced spontaneous firing of the DRN 5-HT neurons. (A) Expression of A-type K + channel-related subfamily members assessed using single-cell PCR analysis in the DRN neurons. (i) Representative image of single-cell PCR products showing the presence of different Kv subunits. (ii) Proportion of Kv1.4, 3.3, 3.4, and Kv4s-positive neurons in the TPH-positive neurons ( n = 20). (B) (i) Confocal images of Kv4.2 and 4.3 protein expression in slice of the DRN, assessed using immunofluorescence methods. Scale bar = 50 μm. (ii) Proportion of Kv4.2 and 4.3-positive neurons in the TPH-positive neurons ( n = 200). (C) PE potently inhibited A-type currents recorded using whole-cell patch clamp in the DRN 5-HT neurons. (i) Recording protocol used and the typical current traces recorded; the latter were from –20 mV. The current amplitude at the beginning of the –20 mV step was measured (dotted square, enlarged in inset). (ii) Time course for current amplitudes measured in (i) . (iii) Summarized data for experiments shown in (i,ii) . Paired t -test, ** p

    Techniques Used: Expressing, Polymerase Chain Reaction, Immunofluorescence, Patch Clamp

    4) Product Images from "Cellular and Subcellular Localisation of Kv4-Associated KChIP Proteins in the Rat Cerebellum"

    Article Title: Cellular and Subcellular Localisation of Kv4-Associated KChIP Proteins in the Rat Cerebellum

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21176403

    Regional and cellular distribution of voltage-gated potassium (Kv) channel subunits Kv4.2 and Kv4.3 in the cerebellum. ( A – D ) Immunoreactivity for Kv4.2 and Kv4.3 in the rat cerebellar cortex using a pre-embedding immunoperoxidase method at the light microscopic level. Parasagittal photomicrographs of the cerebellar cortex. The strongest immunoreactivity for Kv4.2 and Kv4.3 was found in the granule cell layer (gcl). Strong immunoreactivity for Kv4.3 was also observed in the molecular layer (ml), but weaker for Kv4.2. The white matter (wm) was always devoid of any immunolabelling. Immunoreactivity for Kv4.2 and Kv4.3 in the molecular layer was mostly neuropilar, but Kv4.3 labelling was also detected in cell bodies and dendrites of basket cells (black arrows). In the granule cell layer, Kv4.2 and Kv4.3 immunolabelling particularly concentrated in glomeruli (white arrows) and surrounding GCs. Scale bars: ( A , B ), 50 µm; ( C , D ), 25 µm.
    Figure Legend Snippet: Regional and cellular distribution of voltage-gated potassium (Kv) channel subunits Kv4.2 and Kv4.3 in the cerebellum. ( A – D ) Immunoreactivity for Kv4.2 and Kv4.3 in the rat cerebellar cortex using a pre-embedding immunoperoxidase method at the light microscopic level. Parasagittal photomicrographs of the cerebellar cortex. The strongest immunoreactivity for Kv4.2 and Kv4.3 was found in the granule cell layer (gcl). Strong immunoreactivity for Kv4.3 was also observed in the molecular layer (ml), but weaker for Kv4.2. The white matter (wm) was always devoid of any immunolabelling. Immunoreactivity for Kv4.2 and Kv4.3 in the molecular layer was mostly neuropilar, but Kv4.3 labelling was also detected in cell bodies and dendrites of basket cells (black arrows). In the granule cell layer, Kv4.2 and Kv4.3 immunolabelling particularly concentrated in glomeruli (white arrows) and surrounding GCs. Scale bars: ( A , B ), 50 µm; ( C , D ), 25 µm.

    Techniques Used:

    Subcellular distribution of voltage-gated potassium (Kv) channel subunits Kv4.2 and Kv4.3 channels. Immunoreactivity for Kv4.2 and Kv4.3 in the cerebellar cortex as demonstrated by pre-embedding immunogold labelling. ( A – D ) Immunoparticles for both Kv4.2 and Kv4.3 in the molecular layer were found in the plasma membrane (arrows) and intracellular sites (crossed arrows) of PC dendrites (Den) and spines (s) establishing synapses with parallel fibres (pf). Although, at low frequency, Kv4.3 immunoparticles were also found in the plasma membrane (arrowheads) of parallel fibres (pf). ( E – J ) Immunoparticles for both Kv4.2 and Kv4.3 in the granule cell layer were found in the plasma membrane (arrows) of GC somata and GC dendrites (Den) in cerebellar glomeruli. In addition, immunolabelling was found intracellularly (crossed arrows). Few immunoparticles were also observed presynaptically along the plasma membrane (arrowheads) and intracellular sites (double arrowheads) in mossy fibre (mf) axon terminals. Scale bars: ( A – J ), 200 nm.
    Figure Legend Snippet: Subcellular distribution of voltage-gated potassium (Kv) channel subunits Kv4.2 and Kv4.3 channels. Immunoreactivity for Kv4.2 and Kv4.3 in the cerebellar cortex as demonstrated by pre-embedding immunogold labelling. ( A – D ) Immunoparticles for both Kv4.2 and Kv4.3 in the molecular layer were found in the plasma membrane (arrows) and intracellular sites (crossed arrows) of PC dendrites (Den) and spines (s) establishing synapses with parallel fibres (pf). Although, at low frequency, Kv4.3 immunoparticles were also found in the plasma membrane (arrowheads) of parallel fibres (pf). ( E – J ) Immunoparticles for both Kv4.2 and Kv4.3 in the granule cell layer were found in the plasma membrane (arrows) of GC somata and GC dendrites (Den) in cerebellar glomeruli. In addition, immunolabelling was found intracellularly (crossed arrows). Few immunoparticles were also observed presynaptically along the plasma membrane (arrowheads) and intracellular sites (double arrowheads) in mossy fibre (mf) axon terminals. Scale bars: ( A – J ), 200 nm.

    Techniques Used:

    Compartmentalisation of voltage-gated potassium (Kv) channel subunits Kv4.2 and Kv4.3 in cerebellar cells. ( A , B ) Bar graphs showing the percentage of immunoparticles for Kv4.2 and Kv4.3 in neuronal compartment in the molecular layer. Immunoparticles for Kv4.2 were mostly localised at the postsynaptic compartment (99% of all particles), while Kv4.3 was distributed at postsynaptic (88.4%) and presynaptic (11.6%) compartments. ( C , D ) Bar graphs showing the percentage of immunoparticles for Kv4.2 and Kv4.3 in the neuronal compartment in the granule cell layer. A total of 667 immunoparticles for Kv4.2 and 771 for Kv4.3 were analysed. Postsynaptically, immunoparticles were detected in dendrites of GCs (91.6% for Kv4.2; 82.2% for Kv4.3), distributed along the plasma membrane (46.3% for Kv4.2; 62.3% for Kv4.3) and at cytoplasmic sites (53.7% for Kv4.2; 37.7% for Kv4.3). Presynaptically, immunoparticles were detected in mossy fibre terminals (8.4% for Kv4.2; 17.8% for Kv4.3), distributed at cytoplasmic sites (87.5% for Kv4.2; 53.3% for Kv4.3) and along the plasma membrane (12.5% for Kv4.2; 46.7% for Kv4.3). ( E , F ) Histogram showing the distribution of immunoparticles for Kv4.2 and Kv4.3 in relation to glutamate release sites in dendritic spines of PCs. About 46% of immunolabelled Kv4.2 and 52% of immunolabelled Kv4.3 were located in a 60–300 nm wide band. These data show that Kv4.2 immunoparticles were more equally distributed along PC spines, while immunoparticles Kv4.3 were skewed toward the PSD of PC spines.
    Figure Legend Snippet: Compartmentalisation of voltage-gated potassium (Kv) channel subunits Kv4.2 and Kv4.3 in cerebellar cells. ( A , B ) Bar graphs showing the percentage of immunoparticles for Kv4.2 and Kv4.3 in neuronal compartment in the molecular layer. Immunoparticles for Kv4.2 were mostly localised at the postsynaptic compartment (99% of all particles), while Kv4.3 was distributed at postsynaptic (88.4%) and presynaptic (11.6%) compartments. ( C , D ) Bar graphs showing the percentage of immunoparticles for Kv4.2 and Kv4.3 in the neuronal compartment in the granule cell layer. A total of 667 immunoparticles for Kv4.2 and 771 for Kv4.3 were analysed. Postsynaptically, immunoparticles were detected in dendrites of GCs (91.6% for Kv4.2; 82.2% for Kv4.3), distributed along the plasma membrane (46.3% for Kv4.2; 62.3% for Kv4.3) and at cytoplasmic sites (53.7% for Kv4.2; 37.7% for Kv4.3). Presynaptically, immunoparticles were detected in mossy fibre terminals (8.4% for Kv4.2; 17.8% for Kv4.3), distributed at cytoplasmic sites (87.5% for Kv4.2; 53.3% for Kv4.3) and along the plasma membrane (12.5% for Kv4.2; 46.7% for Kv4.3). ( E , F ) Histogram showing the distribution of immunoparticles for Kv4.2 and Kv4.3 in relation to glutamate release sites in dendritic spines of PCs. About 46% of immunolabelled Kv4.2 and 52% of immunolabelled Kv4.3 were located in a 60–300 nm wide band. These data show that Kv4.2 immunoparticles were more equally distributed along PC spines, while immunoparticles Kv4.3 were skewed toward the PSD of PC spines.

    Techniques Used:

    5) Product Images from "Memory Decline and Behavioral Inflexibility in Aged Mice Are Correlated With Dysregulation of Protein Synthesis Capacity"

    Article Title: Memory Decline and Behavioral Inflexibility in Aged Mice Are Correlated With Dysregulation of Protein Synthesis Capacity

    Journal: Frontiers in Aging Neuroscience

    doi: 10.3389/fnagi.2019.00246

    Dysregulations of protein synthesis capacity in aged mice. Western blot performed on hippocampal tissues demonstrated that compared to young mice, old mice exhibited: (A) Increased levels of mTOR phosphorylation (Ser2448); (B) Increased levels of p70S6K phosphorylation (Thr389); (C) Unaltered levels of 4EBP1 phosphorylation; (D) Increased levels of AKT phosphorylation (Ser473); (E) Increased levels of GSK3β (Ser9); (F) Increased levels of eEF2 phosphorylation (Thr56); (G) Increased levels of AMPKα phosphorylation (Thr172); (H) Unaltered levels of Kv4.2 phosphorylation; (I) Decreased levels of GluA1 expression. Except for GluA1, no change on levels of total proteins was observed between young and old mice. n = 7 for young mice and n = 6 for old mice (representative bands from three mice per group). ∗ p
    Figure Legend Snippet: Dysregulations of protein synthesis capacity in aged mice. Western blot performed on hippocampal tissues demonstrated that compared to young mice, old mice exhibited: (A) Increased levels of mTOR phosphorylation (Ser2448); (B) Increased levels of p70S6K phosphorylation (Thr389); (C) Unaltered levels of 4EBP1 phosphorylation; (D) Increased levels of AKT phosphorylation (Ser473); (E) Increased levels of GSK3β (Ser9); (F) Increased levels of eEF2 phosphorylation (Thr56); (G) Increased levels of AMPKα phosphorylation (Thr172); (H) Unaltered levels of Kv4.2 phosphorylation; (I) Decreased levels of GluA1 expression. Except for GluA1, no change on levels of total proteins was observed between young and old mice. n = 7 for young mice and n = 6 for old mice (representative bands from three mice per group). ∗ p

    Techniques Used: Mouse Assay, Western Blot, Expressing

    6) Product Images from "Memory Decline and Behavioral Inflexibility in Aged Mice Are Correlated With Dysregulation of Protein Synthesis Capacity"

    Article Title: Memory Decline and Behavioral Inflexibility in Aged Mice Are Correlated With Dysregulation of Protein Synthesis Capacity

    Journal: Frontiers in Aging Neuroscience

    doi: 10.3389/fnagi.2019.00246

    Dysregulations of protein synthesis capacity in aged mice. Western blot performed on hippocampal tissues demonstrated that compared to young mice, old mice exhibited: (A) Increased levels of mTOR phosphorylation (Ser2448); (B) Increased levels of p70S6K phosphorylation (Thr389); (C) Unaltered levels of 4EBP1 phosphorylation; (D) Increased levels of AKT phosphorylation (Ser473); (E) Increased levels of GSK3β (Ser9); (F) Increased levels of eEF2 phosphorylation (Thr56); (G) Increased levels of AMPKα phosphorylation (Thr172); (H) Unaltered levels of Kv4.2 phosphorylation; (I) Decreased levels of GluA1 expression. Except for GluA1, no change on levels of total proteins was observed between young and old mice. n = 7 for young mice and n = 6 for old mice (representative bands from three mice per group). ∗ p
    Figure Legend Snippet: Dysregulations of protein synthesis capacity in aged mice. Western blot performed on hippocampal tissues demonstrated that compared to young mice, old mice exhibited: (A) Increased levels of mTOR phosphorylation (Ser2448); (B) Increased levels of p70S6K phosphorylation (Thr389); (C) Unaltered levels of 4EBP1 phosphorylation; (D) Increased levels of AKT phosphorylation (Ser473); (E) Increased levels of GSK3β (Ser9); (F) Increased levels of eEF2 phosphorylation (Thr56); (G) Increased levels of AMPKα phosphorylation (Thr172); (H) Unaltered levels of Kv4.2 phosphorylation; (I) Decreased levels of GluA1 expression. Except for GluA1, no change on levels of total proteins was observed between young and old mice. n = 7 for young mice and n = 6 for old mice (representative bands from three mice per group). ∗ p

    Techniques Used: Mouse Assay, Western Blot, Expressing

    7) Product Images from "Memory Decline and Behavioral Inflexibility in Aged Mice Are Correlated With Dysregulation of Protein Synthesis Capacity"

    Article Title: Memory Decline and Behavioral Inflexibility in Aged Mice Are Correlated With Dysregulation of Protein Synthesis Capacity

    Journal: Frontiers in Aging Neuroscience

    doi: 10.3389/fnagi.2019.00246

    Dysregulations of protein synthesis capacity in aged mice. Western blot performed on hippocampal tissues demonstrated that compared to young mice, old mice exhibited: (A) Increased levels of mTOR phosphorylation (Ser2448); (B) Increased levels of p70S6K phosphorylation (Thr389); (C) Unaltered levels of 4EBP1 phosphorylation; (D) Increased levels of AKT phosphorylation (Ser473); (E) Increased levels of GSK3β (Ser9); (F) Increased levels of eEF2 phosphorylation (Thr56); (G) Increased levels of AMPKα phosphorylation (Thr172); (H) Unaltered levels of Kv4.2 phosphorylation; (I) Decreased levels of GluA1 expression. Except for GluA1, no change on levels of total proteins was observed between young and old mice. n = 7 for young mice and n = 6 for old mice (representative bands from three mice per group). ∗ p
    Figure Legend Snippet: Dysregulations of protein synthesis capacity in aged mice. Western blot performed on hippocampal tissues demonstrated that compared to young mice, old mice exhibited: (A) Increased levels of mTOR phosphorylation (Ser2448); (B) Increased levels of p70S6K phosphorylation (Thr389); (C) Unaltered levels of 4EBP1 phosphorylation; (D) Increased levels of AKT phosphorylation (Ser473); (E) Increased levels of GSK3β (Ser9); (F) Increased levels of eEF2 phosphorylation (Thr56); (G) Increased levels of AMPKα phosphorylation (Thr172); (H) Unaltered levels of Kv4.2 phosphorylation; (I) Decreased levels of GluA1 expression. Except for GluA1, no change on levels of total proteins was observed between young and old mice. n = 7 for young mice and n = 6 for old mice (representative bands from three mice per group). ∗ p

    Techniques Used: Mouse Assay, Western Blot, Expressing

    8) Product Images from "Memory Decline and Behavioral Inflexibility in Aged Mice Are Correlated With Dysregulation of Protein Synthesis Capacity"

    Article Title: Memory Decline and Behavioral Inflexibility in Aged Mice Are Correlated With Dysregulation of Protein Synthesis Capacity

    Journal: Frontiers in Aging Neuroscience

    doi: 10.3389/fnagi.2019.00246

    Dysregulations of protein synthesis capacity in aged mice. Western blot performed on hippocampal tissues demonstrated that compared to young mice, old mice exhibited: (A) Increased levels of mTOR phosphorylation (Ser2448); (B) Increased levels of p70S6K phosphorylation (Thr389); (C) Unaltered levels of 4EBP1 phosphorylation; (D) Increased levels of AKT phosphorylation (Ser473); (E) Increased levels of GSK3β (Ser9); (F) Increased levels of eEF2 phosphorylation (Thr56); (G) Increased levels of AMPKα phosphorylation (Thr172); (H) Unaltered levels of Kv4.2 phosphorylation; (I) Decreased levels of GluA1 expression. Except for GluA1, no change on levels of total proteins was observed between young and old mice. n = 7 for young mice and n = 6 for old mice (representative bands from three mice per group). ∗ p
    Figure Legend Snippet: Dysregulations of protein synthesis capacity in aged mice. Western blot performed on hippocampal tissues demonstrated that compared to young mice, old mice exhibited: (A) Increased levels of mTOR phosphorylation (Ser2448); (B) Increased levels of p70S6K phosphorylation (Thr389); (C) Unaltered levels of 4EBP1 phosphorylation; (D) Increased levels of AKT phosphorylation (Ser473); (E) Increased levels of GSK3β (Ser9); (F) Increased levels of eEF2 phosphorylation (Thr56); (G) Increased levels of AMPKα phosphorylation (Thr172); (H) Unaltered levels of Kv4.2 phosphorylation; (I) Decreased levels of GluA1 expression. Except for GluA1, no change on levels of total proteins was observed between young and old mice. n = 7 for young mice and n = 6 for old mice (representative bands from three mice per group). ∗ p

    Techniques Used: Mouse Assay, Western Blot, Expressing

    9) Product Images from "Transient Outward K+ Current (Ito) Underlies the Right Ventricular Initiation of Polymorphic Ventricular Tachycardia in a Transgenic Rabbit Model of Long QT Type 1"

    Article Title: Transient Outward K+ Current (Ito) Underlies the Right Ventricular Initiation of Polymorphic Ventricular Tachycardia in a Transgenic Rabbit Model of Long QT Type 1

    Journal: Circulation. Arrhythmia and electrophysiology

    doi: 10.1161/CIRCEP.117.005414

    I to recovery from inactivation. A) The recovery kinetics was tested by a double-pulse protocol with interpulse time varying from 50 ms to 15 sec (n=12 RV and 7 LV cells from n=3 hearts). B) The amplitudes of the slow and fast inactivating components of I to (I to,si and I I to,fi ) as a function of inter-pulse interval were determined by fitting the time course of I to decay during the second pulse to a double exponential function. The x-axis of inter-pulse intervals is in a logarithmic scale. C) The amplitudes of I to,fi and I to,si from RV and LV. Fast and slow-inactivating components (I to,fi and I to,si ) of each I to,f and I to,s were calculated as described in Methods and represented as a stacked column plot. D) Western blots of Kv4.2, Kv1.4, and KChIP2 from LQT1 hearts. E). The accessory unit of I to , KChIP2, known to affect inactivation and recovery kinetics, was twofold higher in RV (ANOVA, p .
    Figure Legend Snippet: I to recovery from inactivation. A) The recovery kinetics was tested by a double-pulse protocol with interpulse time varying from 50 ms to 15 sec (n=12 RV and 7 LV cells from n=3 hearts). B) The amplitudes of the slow and fast inactivating components of I to (I to,si and I I to,fi ) as a function of inter-pulse interval were determined by fitting the time course of I to decay during the second pulse to a double exponential function. The x-axis of inter-pulse intervals is in a logarithmic scale. C) The amplitudes of I to,fi and I to,si from RV and LV. Fast and slow-inactivating components (I to,fi and I to,si ) of each I to,f and I to,s were calculated as described in Methods and represented as a stacked column plot. D) Western blots of Kv4.2, Kv1.4, and KChIP2 from LQT1 hearts. E). The accessory unit of I to , KChIP2, known to affect inactivation and recovery kinetics, was twofold higher in RV (ANOVA, p .

    Techniques Used: Mass Spectrometry, Size-exclusion Chromatography, Western Blot

    10) Product Images from "Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus"

    Article Title: Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20020246

    Distribution of immunoreactivity for Kv4.2 in the hippocampus. ( A ) At the light microscopic level, immunoreactivity for Kv4.2 was widely distributed in the hippocampus but its intensity varied consistently; ( B ) In the CA1 region, immunolabelling for Kv4.2 was generally moderate-to-strong, with the strata oriens (so) and radiatum (sr) showing the highest and the stratum lacunosum-moleculare (slm) showing weak immunoreactivity. The weakest immunolabelling for Kv4.2 was observed in the stratum pyramidale (sp); ( C ) In the CA3 region, moderate immunolabelling for Kv4.2 was observed in the strata oriens (so), radiatum (sr) and stratum lacunosum-moleculare (slm), weak in the stratum lucidum and very weak in the stratum pyramidale (sp). Kv4.2 immunolabelling was also seen outlining somata and dendrites of scattered interneurons (arrows); ( D ) In the dentate gyrus (DG), immunolabelling was strong in the molecular layer (ml), weak in the hilus (h) and the weakest in the granule cell layer (gc). Scale bars: A , 500 µm; B – D , 150 µm.
    Figure Legend Snippet: Distribution of immunoreactivity for Kv4.2 in the hippocampus. ( A ) At the light microscopic level, immunoreactivity for Kv4.2 was widely distributed in the hippocampus but its intensity varied consistently; ( B ) In the CA1 region, immunolabelling for Kv4.2 was generally moderate-to-strong, with the strata oriens (so) and radiatum (sr) showing the highest and the stratum lacunosum-moleculare (slm) showing weak immunoreactivity. The weakest immunolabelling for Kv4.2 was observed in the stratum pyramidale (sp); ( C ) In the CA3 region, moderate immunolabelling for Kv4.2 was observed in the strata oriens (so), radiatum (sr) and stratum lacunosum-moleculare (slm), weak in the stratum lucidum and very weak in the stratum pyramidale (sp). Kv4.2 immunolabelling was also seen outlining somata and dendrites of scattered interneurons (arrows); ( D ) In the dentate gyrus (DG), immunolabelling was strong in the molecular layer (ml), weak in the hilus (h) and the weakest in the granule cell layer (gc). Scale bars: A , 500 µm; B – D , 150 µm.

    Techniques Used:

    Subcellular localisation of Kv4.2 in the adult hippocampus. Electron micrographs showing immunoparticles for Kv4.2 in the hippocampus, as detected using the pre-embedding immunogold technique at P60. ( A – D ) Immunoreactivity for Kv4.2 was detected in similar neuronal compartments in the CA1 (panels A , B and C ) and CA3 (panels D and E ) regions and dentate gyrus (DG, panels F and G ). Kv4.2 immunoparticles were abundant along the extrasynaptic plasma membrane (arrows) of dendritic spines (s) contacted by axon terminals (at) and dendritic shafts (Den) of pyramidal cells and granule cells. Immunoparticles were also observed at intracellular sites (crossed arrows) in dendritic spines (s) and shafts (Den). Very few immunoparticles for Kv4.2 were also localised to the extrasynaptic plasma membrane (arrowheads) of axon terminals (at) establishing asymmetrical synapses with spines (s). Kv4.2 immunoparticles (double arrowheads) were detected along the main body of the postsynaptic membrane specialisation of GABAergic synapses in the CA1 (panel C ), CA3 (panel E ) and DG (panel G ); ( H ) Compartmentalisation of Kv4.2 in CA1 pyramidal cells, CA3 pyramidal cells and DG granule cells. Bar graphs showing the percentage of immunoparticles for Kv4.2 at post- and presynaptic compartments, and along the plasma membrane and intracellular sites in dendritic spines, dendritic shafts and axon terminals. A total of 1355 immunoparticles in the CA1, 1223 in the CA3 and 1037 in the DG were analysed. Most immunoparticles were postsynaptic, both along the plasma membrane and at cytoplasmic sites. Postsynaptically, immunoparticles were detected in dendritic spines and in dendritic shafts; ( I ) Histogram showing the distribution of immunoreactive Kv4.2 in relation to glutamate release sites in dendritic spines of CA1 and CA3 pyramidal cells, and DG granule cells. Data are expressed as the proportion of immunoparticles at a given distance from the edge of the synaptic specialisation. These data show that Kv4.2 immunoparticles were distributed similarly in spines in CA1, CA3 and DG and in the proximity of asymmetrical synapses on dendritic spines. Scale bars: A , D , F , G , 200 nm; B , C , E , 500 nm.
    Figure Legend Snippet: Subcellular localisation of Kv4.2 in the adult hippocampus. Electron micrographs showing immunoparticles for Kv4.2 in the hippocampus, as detected using the pre-embedding immunogold technique at P60. ( A – D ) Immunoreactivity for Kv4.2 was detected in similar neuronal compartments in the CA1 (panels A , B and C ) and CA3 (panels D and E ) regions and dentate gyrus (DG, panels F and G ). Kv4.2 immunoparticles were abundant along the extrasynaptic plasma membrane (arrows) of dendritic spines (s) contacted by axon terminals (at) and dendritic shafts (Den) of pyramidal cells and granule cells. Immunoparticles were also observed at intracellular sites (crossed arrows) in dendritic spines (s) and shafts (Den). Very few immunoparticles for Kv4.2 were also localised to the extrasynaptic plasma membrane (arrowheads) of axon terminals (at) establishing asymmetrical synapses with spines (s). Kv4.2 immunoparticles (double arrowheads) were detected along the main body of the postsynaptic membrane specialisation of GABAergic synapses in the CA1 (panel C ), CA3 (panel E ) and DG (panel G ); ( H ) Compartmentalisation of Kv4.2 in CA1 pyramidal cells, CA3 pyramidal cells and DG granule cells. Bar graphs showing the percentage of immunoparticles for Kv4.2 at post- and presynaptic compartments, and along the plasma membrane and intracellular sites in dendritic spines, dendritic shafts and axon terminals. A total of 1355 immunoparticles in the CA1, 1223 in the CA3 and 1037 in the DG were analysed. Most immunoparticles were postsynaptic, both along the plasma membrane and at cytoplasmic sites. Postsynaptically, immunoparticles were detected in dendritic spines and in dendritic shafts; ( I ) Histogram showing the distribution of immunoreactive Kv4.2 in relation to glutamate release sites in dendritic spines of CA1 and CA3 pyramidal cells, and DG granule cells. Data are expressed as the proportion of immunoparticles at a given distance from the edge of the synaptic specialisation. These data show that Kv4.2 immunoparticles were distributed similarly in spines in CA1, CA3 and DG and in the proximity of asymmetrical synapses on dendritic spines. Scale bars: A , D , F , G , 200 nm; B , C , E , 500 nm.

    Techniques Used:

    Co-localisation of Kv4.2 and Kv4.3 in granule cells. ( A – C ) Electron micrographs showing co-localisation for Kv4.2 with Kv4.3, as detected using a double-labelling pre-embedding immunogold method at P60. Labelling is shown with immunoperoxidase reaction for Kv4.2, and with silver-intensified immunogold reaction for Kv4.3. Immunoparticles for Kv4.3 were seen in dendritic spines (s) and shafts (Den) of granule cells immunopositive for Kv4.2, both along the plasma membrane (arrows) and at intracellular sites (crossed arrows); ( D ) Change in the density of Kv4.2 and Kv4.3 in DG granule cells as a function of distance from the soma in six somato-dendritic domains. Density of immunoparticles for the two channel subtypes increased significantly from soma to dendritic spines (Sp) in the inner one-third and outer two-thirds of the molecular layer. This analysis demonstrated their similar non-uniform distributions over the neuronal surface of granule cells. Inner SB, spiny branchlets in the inner one-third; Outer SB, spiny branchlets in the outer two-third; Inner Sp, spines in the inner one-third; Outer Sp, spines in the outer two-third. Scale bars: A – C , 500 nm.
    Figure Legend Snippet: Co-localisation of Kv4.2 and Kv4.3 in granule cells. ( A – C ) Electron micrographs showing co-localisation for Kv4.2 with Kv4.3, as detected using a double-labelling pre-embedding immunogold method at P60. Labelling is shown with immunoperoxidase reaction for Kv4.2, and with silver-intensified immunogold reaction for Kv4.3. Immunoparticles for Kv4.3 were seen in dendritic spines (s) and shafts (Den) of granule cells immunopositive for Kv4.2, both along the plasma membrane (arrows) and at intracellular sites (crossed arrows); ( D ) Change in the density of Kv4.2 and Kv4.3 in DG granule cells as a function of distance from the soma in six somato-dendritic domains. Density of immunoparticles for the two channel subtypes increased significantly from soma to dendritic spines (Sp) in the inner one-third and outer two-thirds of the molecular layer. This analysis demonstrated their similar non-uniform distributions over the neuronal surface of granule cells. Inner SB, spiny branchlets in the inner one-third; Outer SB, spiny branchlets in the outer two-third; Inner Sp, spines in the inner one-third; Outer Sp, spines in the outer two-third. Scale bars: A – C , 500 nm.

    Techniques Used:

    Developmental and regional distribution of the Kv4.2 channel in the mouse brain. ( A ) Kv4.2 protein distribution was visualised on histoblots of brain horizontal sections at various stages of postnatal development using an affinity-purified anti-Kv4.2 antibody. Kv4.2 was expressed in the brain since the day of birth (P0), and at all stages the strongest expression was detected in the cerebellum (Cb), caudate putamen (CPu), hippocampus (Hp) and thalamus (Th), with the lowest intensity in the cortex (Cx) and septum (Sp); ( B ) The histoblots were scanned and densitometric measurements from four independent experiments were averaged to compare the protein densities for each developmental time point. The analysis revealed a differential Kv4.2 expression in a developmental stage- and region-specific manner. Kv4.2 expression was detected at P0, increased progressively to reach a peak at P10, P15 or P21 depending on the brain region, and then decreasing at P30. Error bars indicate SEM. Scale bars, 2 mm.
    Figure Legend Snippet: Developmental and regional distribution of the Kv4.2 channel in the mouse brain. ( A ) Kv4.2 protein distribution was visualised on histoblots of brain horizontal sections at various stages of postnatal development using an affinity-purified anti-Kv4.2 antibody. Kv4.2 was expressed in the brain since the day of birth (P0), and at all stages the strongest expression was detected in the cerebellum (Cb), caudate putamen (CPu), hippocampus (Hp) and thalamus (Th), with the lowest intensity in the cortex (Cx) and septum (Sp); ( B ) The histoblots were scanned and densitometric measurements from four independent experiments were averaged to compare the protein densities for each developmental time point. The analysis revealed a differential Kv4.2 expression in a developmental stage- and region-specific manner. Kv4.2 expression was detected at P0, increased progressively to reach a peak at P10, P15 or P21 depending on the brain region, and then decreasing at P30. Error bars indicate SEM. Scale bars, 2 mm.

    Techniques Used: Affinity Purification, Expressing

    Regional distribution of the Kv4.2 channel in the adult mouse brain. ( A , B ) The distribution of the Kv4.2 protein was visualised in histoblots of horizontal brain sections at P60 using an affinity-purified anti-Kv4.2 antibody. The expression of Kv4.2 in different brain regions was determined by densitometric analysis of the scanned histoblots. The strongest expression was detected in the cerebellum (Cb) and hippocampus (Hp), with moderate expression in the caudate putamen (CPu) and thalamus (Th). Weak expression level was detected in the cortex (Cx) and septum (Sp); ( C , D ) In the hippocampus, very strong Kv4.2 expression was detected in the strata oriens (so) and radiatum (sr) of the CA1 region and the molecular layer (ml) of the dentate gyrus (DG); ( C , D ) Moderate staining was observed in all dendritic layers of CA3, in the stratum lacunosum-moleculare (slm) of the CA1 region and the hilus of the dentate gyrus. Very weak Kv4.2 staining was observed in the stratum pyramidale of the CA1 and CA3 regions and in the granule cell layer of the dentate gyrusso, stratum oriens ; sr, stratum radiatum ; DG, dentate gyrus; h, hilus .; ( E , F ) In the cerebellum, the strongest expression level was detected in the granule cell layer (gc), with weak expression in the molecular layer (ml) and very weak in the white matter (wm). Error bars indicate SEM; * p
    Figure Legend Snippet: Regional distribution of the Kv4.2 channel in the adult mouse brain. ( A , B ) The distribution of the Kv4.2 protein was visualised in histoblots of horizontal brain sections at P60 using an affinity-purified anti-Kv4.2 antibody. The expression of Kv4.2 in different brain regions was determined by densitometric analysis of the scanned histoblots. The strongest expression was detected in the cerebellum (Cb) and hippocampus (Hp), with moderate expression in the caudate putamen (CPu) and thalamus (Th). Weak expression level was detected in the cortex (Cx) and septum (Sp); ( C , D ) In the hippocampus, very strong Kv4.2 expression was detected in the strata oriens (so) and radiatum (sr) of the CA1 region and the molecular layer (ml) of the dentate gyrus (DG); ( C , D ) Moderate staining was observed in all dendritic layers of CA3, in the stratum lacunosum-moleculare (slm) of the CA1 region and the hilus of the dentate gyrus. Very weak Kv4.2 staining was observed in the stratum pyramidale of the CA1 and CA3 regions and in the granule cell layer of the dentate gyrusso, stratum oriens ; sr, stratum radiatum ; DG, dentate gyrus; h, hilus .; ( E , F ) In the cerebellum, the strongest expression level was detected in the granule cell layer (gc), with weak expression in the molecular layer (ml) and very weak in the white matter (wm). Error bars indicate SEM; * p

    Techniques Used: Affinity Purification, Expressing, Staining

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    Alomone Labs rabbit polyclonal anti kv4 2 antibody
    <t>Kv4.3</t> immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with <t>antibodies</t> directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.
    Rabbit Polyclonal Anti Kv4 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti kv4 2 antibody/product/Alomone Labs
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    95
    Alomone Labs anti kv4 3 antibody
    <t>Kv4.3</t> immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with <t>antibodies</t> directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.
    Anti Kv4 3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv4 3 antibody/product/Alomone Labs
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    anti kv4 3 antibody - by Bioz Stars, 2022-12
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    Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.

    Article Snippet: The following antibodies were used for immunostaining only (all at a dilution of 1:400): rabbit polyclonal anti-Kv4.2 antibody (Alomone, RRID:AB_2040176 ) generated against amino acids 454–469 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877281 ) generated against a synthetic peptide corresponding to amino acids 209–225 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877425 ) generated against a fusion protein corresponding to amino acids 471-630 of rat Kv4.2; rabbit polyclonal anti-Kv1.4 antibody (Alomone, RRID:AB_2040153 ) generated against a GST fusion protein corresponding to amino acid 589-655 of rat Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877317 ) generated against a synthetic peptide corresponding to amino acids 13–37 of rat brain Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877393 ) generated against a fusion peptide of amino acids 336-370 of rat Kv1.4; and mouse monoclonal IgG1 anti-NF-L antibody (Millipore MAB1615) directed against enzymatically dephosphorylated pig neurofilament-L (70 kDa).

    Techniques:

    Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.

    Article Snippet: The following antibodies were used for immunostaining only (all at a dilution of 1:400): rabbit polyclonal anti-Kv4.2 antibody (Alomone, RRID:AB_2040176 ) generated against amino acids 454–469 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877281 ) generated against a synthetic peptide corresponding to amino acids 209–225 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877425 ) generated against a fusion protein corresponding to amino acids 471-630 of rat Kv4.2; rabbit polyclonal anti-Kv1.4 antibody (Alomone, RRID:AB_2040153 ) generated against a GST fusion protein corresponding to amino acid 589-655 of rat Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877317 ) generated against a synthetic peptide corresponding to amino acids 13–37 of rat brain Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877393 ) generated against a fusion peptide of amino acids 336-370 of rat Kv1.4; and mouse monoclonal IgG1 anti-NF-L antibody (Millipore MAB1615) directed against enzymatically dephosphorylated pig neurofilament-L (70 kDa).

    Techniques: Binding Assay, Microscopy, Western Blot, Fluorescence, Molecular Weight, Migration

    Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.

    Article Snippet: The following antibodies were used for immunostaining only (all at a dilution of 1:400): rabbit polyclonal anti-Kv4.2 antibody (Alomone, RRID:AB_2040176 ) generated against amino acids 454–469 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877281 ) generated against a synthetic peptide corresponding to amino acids 209–225 of rat Kv4.2; mouse monoclonal IgG1 anti-Kv4.2 antibody (NeuroMab, RRID:AB_2877425 ) generated against a fusion protein corresponding to amino acids 471-630 of rat Kv4.2; rabbit polyclonal anti-Kv1.4 antibody (Alomone, RRID:AB_2040153 ) generated against a GST fusion protein corresponding to amino acid 589-655 of rat Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877317 ) generated against a synthetic peptide corresponding to amino acids 13–37 of rat brain Kv1.4; mouse monoclonal IgG1 anti-Kv1.4 antibody (NeuroMab, RRID:AB_2877393 ) generated against a fusion peptide of amino acids 336-370 of rat Kv1.4; and mouse monoclonal IgG1 anti-NF-L antibody (Millipore MAB1615) directed against enzymatically dephosphorylated pig neurofilament-L (70 kDa).

    Techniques: Immunoprecipitation, Western Blot, Electrophoresis, Molecular Weight, Migration

    Expression of A-type K + channels and their contribution to the PE-induced spontaneous firing of the DRN 5-HT neurons. (A) Expression of A-type K + channel-related subfamily members assessed using single-cell PCR analysis in the DRN neurons. (i) Representative image of single-cell PCR products showing the presence of different Kv subunits. (ii) Proportion of Kv1.4, 3.3, 3.4, and Kv4s-positive neurons in the TPH-positive neurons ( n = 20). (B) (i) Confocal images of Kv4.2 and 4.3 protein expression in slice of the DRN, assessed using immunofluorescence methods. Scale bar = 50 μm. (ii) Proportion of Kv4.2 and 4.3-positive neurons in the TPH-positive neurons ( n = 200). (C) PE potently inhibited A-type currents recorded using whole-cell patch clamp in the DRN 5-HT neurons. (i) Recording protocol used and the typical current traces recorded; the latter were from –20 mV. The current amplitude at the beginning of the –20 mV step was measured (dotted square, enlarged in inset). (ii) Time course for current amplitudes measured in (i) . (iii) Summarized data for experiments shown in (i,ii) . Paired t -test, ** p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Potassium Channel Conductance Is Involved in Phenylephrine-Induced Spontaneous Firing of Serotonergic Neurons in the Dorsal Raphe Nucleus

    doi: 10.3389/fncel.2022.891912

    Figure Lengend Snippet: Expression of A-type K + channels and their contribution to the PE-induced spontaneous firing of the DRN 5-HT neurons. (A) Expression of A-type K + channel-related subfamily members assessed using single-cell PCR analysis in the DRN neurons. (i) Representative image of single-cell PCR products showing the presence of different Kv subunits. (ii) Proportion of Kv1.4, 3.3, 3.4, and Kv4s-positive neurons in the TPH-positive neurons ( n = 20). (B) (i) Confocal images of Kv4.2 and 4.3 protein expression in slice of the DRN, assessed using immunofluorescence methods. Scale bar = 50 μm. (ii) Proportion of Kv4.2 and 4.3-positive neurons in the TPH-positive neurons ( n = 200). (C) PE potently inhibited A-type currents recorded using whole-cell patch clamp in the DRN 5-HT neurons. (i) Recording protocol used and the typical current traces recorded; the latter were from –20 mV. The current amplitude at the beginning of the –20 mV step was measured (dotted square, enlarged in inset). (ii) Time course for current amplitudes measured in (i) . (iii) Summarized data for experiments shown in (i,ii) . Paired t -test, ** p

    Article Snippet: Commercial antibodies used were anti-TPH (1:400, mouse, sigma, T0678, RRID:AB_261587 ), anti-Kv4.2 (1:400, rabbit, AlomoneLabs, APC-023, RRID:AB_2040176 ), anti-Kv4.3 (1:400, rabbit, AlomoneLabs, APC-017, RRID:AB_2040178 ), anti-SK2 (1:200, rabbit, Bioss, DF13499, RRID:AB_2846518 ), anti-SK3 (1:200, rabbit, Proteintech, 17188-1-AP), anti-KCNQ2 (1:200, goat, Santa Cruz, sc-7793, RRID:AB_2296585 ), anti-KCNQ3 (1:200, goat, Santa Cruz, sc-7794, RRID:AB_2131714 ), and anti-KCNQ4 (1:200, rabbit, AlomoneLabs, APC-164, RRID:AB_2341042 ).

    Techniques: Expressing, Polymerase Chain Reaction, Immunofluorescence, Patch Clamp

    Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.

    Article Snippet: The anti-CaMKII antibody immunoprecipitated protein that was detectable by anti-CaMKII antibody ( , lane 4), co-immunoprecipitated protein that was detectable by anti-Kv4.3 antibody ( , lane 2), and bound to protein in the elute from the anti-Kv4.3 antibody pull-down ( , lane 5).

    Techniques:

    Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.

    Article Snippet: The anti-CaMKII antibody immunoprecipitated protein that was detectable by anti-CaMKII antibody ( , lane 4), co-immunoprecipitated protein that was detectable by anti-Kv4.3 antibody ( , lane 2), and bound to protein in the elute from the anti-Kv4.3 antibody pull-down ( , lane 5).

    Techniques: Binding Assay, Microscopy, Western Blot, Fluorescence, Molecular Weight, Migration

    Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.

    Article Snippet: The anti-CaMKII antibody immunoprecipitated protein that was detectable by anti-CaMKII antibody ( , lane 4), co-immunoprecipitated protein that was detectable by anti-Kv4.3 antibody ( , lane 2), and bound to protein in the elute from the anti-Kv4.3 antibody pull-down ( , lane 5).

    Techniques: Immunoprecipitation, Western Blot, Electrophoresis, Molecular Weight, Migration