anti kv1 4 polyclonal antibodies  (Alomone Labs)


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    Alomone Labs anti kv1 4 polyclonal antibodies
    Immunodetection of Kv1.2, <t>Kv1.4,</t> Kv1.5, and Kv1.6 channel proteins in OP cells/OLGs. A , B , Specificity of anti-Kv1.5 and anti-Kv1.6 <t>polyclonal</t> antibodies in transfected HEK 293 cells. Shown are Western blots of extracts from HEK 293 cells transfected
    Anti Kv1 4 Polyclonal Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv1 4 polyclonal antibodies/product/Alomone Labs
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    anti kv1 4 polyclonal antibodies - by Bioz Stars, 2022-09
    92/100 stars

    Images

    1) Product Images from "Characterization of Delayed Rectifier Kv Channels in Oligodendrocytes and Progenitor Cells"

    Article Title: Characterization of Delayed Rectifier Kv Channels in Oligodendrocytes and Progenitor Cells

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.17-21-08234.1997

    Immunodetection of Kv1.2, Kv1.4, Kv1.5, and Kv1.6 channel proteins in OP cells/OLGs. A , B , Specificity of anti-Kv1.5 and anti-Kv1.6 polyclonal antibodies in transfected HEK 293 cells. Shown are Western blots of extracts from HEK 293 cells transfected
    Figure Legend Snippet: Immunodetection of Kv1.2, Kv1.4, Kv1.5, and Kv1.6 channel proteins in OP cells/OLGs. A , B , Specificity of anti-Kv1.5 and anti-Kv1.6 polyclonal antibodies in transfected HEK 293 cells. Shown are Western blots of extracts from HEK 293 cells transfected

    Techniques Used: Immunodetection, Transfection, Western Blot

    2) Product Images from "Colchicine modulates calcium homeostasis and electrical property of HL‐1 cells"

    Article Title: Colchicine modulates calcium homeostasis and electrical property of HL‐1 cells

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.12818

    Effect of colchicine on the protein expression of calcium (Ca 2+ ) regulatory proteins and potassium channel proteins. ( A ) Representative immunoblotting and average data of sarcoplasmic reticulum Ca 2+ ATPase (SERCA2a), Cav1.2, Ca 2+ /calmodulin‐dependent protein kinase II (CaMKII), PLB, Ser16‐ and Thr17‐phosphorylated PLB (PLB‐Ser16 and PLB‐Thr17) from control and colchicine (3 nM)‐treated HL‐1 cells. ( n = 9) ( B ) Representative immunoblotting and average data of RyR type 2, phosphorylation of RyR at S2808 and S2814 (RyR‐2808 and RyR‐2814), the Na + –Ca 2+ exchanger (NCX), Kv1.4, Kv1.5 and Kv4.2 from control and colchicine (3 nM)‐treated HL‐1 cells ( n = 7). * P
    Figure Legend Snippet: Effect of colchicine on the protein expression of calcium (Ca 2+ ) regulatory proteins and potassium channel proteins. ( A ) Representative immunoblotting and average data of sarcoplasmic reticulum Ca 2+ ATPase (SERCA2a), Cav1.2, Ca 2+ /calmodulin‐dependent protein kinase II (CaMKII), PLB, Ser16‐ and Thr17‐phosphorylated PLB (PLB‐Ser16 and PLB‐Thr17) from control and colchicine (3 nM)‐treated HL‐1 cells. ( n = 9) ( B ) Representative immunoblotting and average data of RyR type 2, phosphorylation of RyR at S2808 and S2814 (RyR‐2808 and RyR‐2814), the Na + –Ca 2+ exchanger (NCX), Kv1.4, Kv1.5 and Kv4.2 from control and colchicine (3 nM)‐treated HL‐1 cells ( n = 7). * P

    Techniques Used: Expressing

    3) Product Images from "Characterization of Delayed Rectifier Kv Channels in Oligodendrocytes and Progenitor Cells"

    Article Title: Characterization of Delayed Rectifier Kv Channels in Oligodendrocytes and Progenitor Cells

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.17-21-08234.1997

    Immunodetection of Kv1.2, Kv1.4, Kv1.5, and Kv1.6 channel proteins in OP cells/OLGs. A , B , Specificity of anti-Kv1.5 and anti-Kv1.6 polyclonal antibodies in transfected HEK 293 cells. Shown are Western blots of extracts from HEK 293 cells transfected
    Figure Legend Snippet: Immunodetection of Kv1.2, Kv1.4, Kv1.5, and Kv1.6 channel proteins in OP cells/OLGs. A , B , Specificity of anti-Kv1.5 and anti-Kv1.6 polyclonal antibodies in transfected HEK 293 cells. Shown are Western blots of extracts from HEK 293 cells transfected

    Techniques Used: Immunodetection, Transfection, Western Blot

    4) Product Images from "Characterization of Delayed Rectifier Kv Channels in Oligodendrocytes and Progenitor Cells"

    Article Title: Characterization of Delayed Rectifier Kv Channels in Oligodendrocytes and Progenitor Cells

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.17-21-08234.1997

    Immunodetection of Kv1.2, Kv1.4, Kv1.5, and Kv1.6 channel proteins in OP cells/OLGs. A , B , Specificity of anti-Kv1.5 and anti-Kv1.6 polyclonal antibodies in transfected HEK 293 cells. Shown are Western blots of extracts from HEK 293 cells transfected
    Figure Legend Snippet: Immunodetection of Kv1.2, Kv1.4, Kv1.5, and Kv1.6 channel proteins in OP cells/OLGs. A , B , Specificity of anti-Kv1.5 and anti-Kv1.6 polyclonal antibodies in transfected HEK 293 cells. Shown are Western blots of extracts from HEK 293 cells transfected

    Techniques Used: Immunodetection, Transfection, Western Blot

    5) Product Images from "Colchicine modulates calcium homeostasis and electrical property of HL‐1 cells"

    Article Title: Colchicine modulates calcium homeostasis and electrical property of HL‐1 cells

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.12818

    Effect of colchicine on the protein expression of calcium (Ca 2+ ) regulatory proteins and potassium channel proteins. ( A ) Representative immunoblotting and average data of sarcoplasmic reticulum Ca 2+ ATPase (SERCA2a), Cav1.2, Ca 2+ /calmodulin‐dependent protein kinase II (CaMKII), PLB, Ser16‐ and Thr17‐phosphorylated PLB (PLB‐Ser16 and PLB‐Thr17) from control and colchicine (3 nM)‐treated HL‐1 cells. ( n = 9) ( B ) Representative immunoblotting and average data of RyR type 2, phosphorylation of RyR at S2808 and S2814 (RyR‐2808 and RyR‐2814), the Na + –Ca 2+ exchanger (NCX), Kv1.4, Kv1.5 and Kv4.2 from control and colchicine (3 nM)‐treated HL‐1 cells ( n = 7). * P
    Figure Legend Snippet: Effect of colchicine on the protein expression of calcium (Ca 2+ ) regulatory proteins and potassium channel proteins. ( A ) Representative immunoblotting and average data of sarcoplasmic reticulum Ca 2+ ATPase (SERCA2a), Cav1.2, Ca 2+ /calmodulin‐dependent protein kinase II (CaMKII), PLB, Ser16‐ and Thr17‐phosphorylated PLB (PLB‐Ser16 and PLB‐Thr17) from control and colchicine (3 nM)‐treated HL‐1 cells. ( n = 9) ( B ) Representative immunoblotting and average data of RyR type 2, phosphorylation of RyR at S2808 and S2814 (RyR‐2808 and RyR‐2814), the Na + –Ca 2+ exchanger (NCX), Kv1.4, Kv1.5 and Kv4.2 from control and colchicine (3 nM)‐treated HL‐1 cells ( n = 7). * P

    Techniques Used: Expressing

    6) Product Images from "K+ Channel Alterations in the Progression of Experimental Autoimmune Encephalomyelitis"

    Article Title: K+ Channel Alterations in the Progression of Experimental Autoimmune Encephalomyelitis

    Journal: Neurobiology of Disease

    doi: 10.1016/j.nbd.2012.04.012

    Up-regulated Kv1.4 channels partially colocalized with NG2 but not with YFP-positive axons in Thy1:YFP transgenic mice
    Figure Legend Snippet: Up-regulated Kv1.4 channels partially colocalized with NG2 but not with YFP-positive axons in Thy1:YFP transgenic mice

    Techniques Used: Transgenic Assay, Mouse Assay

    Upregulation of Kv1.4 channels in GFAP-positive cells around EAE lesions
    Figure Legend Snippet: Upregulation of Kv1.4 channels in GFAP-positive cells around EAE lesions

    Techniques Used:

    7) Product Images from "Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats"

    Article Title: Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats

    Journal: CNS Neuroscience & Therapeutics

    doi: 10.1111/j.1755-5949.2010.00133.x

    Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia.  a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp,  b ), Kv 4.1 (467 bp,  c ), Kv 4.2 (110 bp,  d ), Kv 4.3 (400 bp,  e ), and GAPDH (418 bp,  f ). Negative control (no reverse transcription,  g ).
    Figure Legend Snippet: Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia. a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp, b ), Kv 4.1 (467 bp, c ), Kv 4.2 (110 bp, d ), Kv 4.3 (400 bp, e ), and GAPDH (418 bp, f ). Negative control (no reverse transcription, g ).

    Techniques Used: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Negative Control

    8) Product Images from "Astrocytes differentially respond to inflammatory autoimmune insults and imbalances of neural activity"

    Article Title: Astrocytes differentially respond to inflammatory autoimmune insults and imbalances of neural activity

    Journal: Acta Neuropathologica Communications

    doi: 10.1186/2051-5960-1-70

    Activation of astrocytes in spinal cord white matter. A , Clinical scores (top) and body weight (bottom) of mice with chEAE. B , White matter (WM) and gray matter (GM) in spinal cord longitudinal section was stained with FMG (green) and nuclear dye (blue). Box 1 shows both GM and WM and box 2 shows only WM. Spinal cord sections, control (C) and EAE peak (D) , were co-stained for GFAP (green, top), AQP4 (red), Hoechst (blue), and FMG (green, bottom). Co-staining of Kv1.4 (green, top), Vim (red), Hoechst (blue) and FMG (green, bottom) were also performed on control (E) and EAE (F) spinal cord sections. High magnification confocal image stacks were obtained from control (G) and EAE (H) Thy1-YFP transgenic mice. Images contain YFP (green), GFAP (blue) and AQP4 (red). The collapsed 2D image is on the left, and 3 cross sections are on the right. In (G) , the crossbars are centered on a putative node of Ranvier. In (H) , the crossbars are centered on the AQP4+/GFAP + lesion edge. Scale bars, 500 μm in C-F , 50 μm in G , H .
    Figure Legend Snippet: Activation of astrocytes in spinal cord white matter. A , Clinical scores (top) and body weight (bottom) of mice with chEAE. B , White matter (WM) and gray matter (GM) in spinal cord longitudinal section was stained with FMG (green) and nuclear dye (blue). Box 1 shows both GM and WM and box 2 shows only WM. Spinal cord sections, control (C) and EAE peak (D) , were co-stained for GFAP (green, top), AQP4 (red), Hoechst (blue), and FMG (green, bottom). Co-staining of Kv1.4 (green, top), Vim (red), Hoechst (blue) and FMG (green, bottom) were also performed on control (E) and EAE (F) spinal cord sections. High magnification confocal image stacks were obtained from control (G) and EAE (H) Thy1-YFP transgenic mice. Images contain YFP (green), GFAP (blue) and AQP4 (red). The collapsed 2D image is on the left, and 3 cross sections are on the right. In (G) , the crossbars are centered on a putative node of Ranvier. In (H) , the crossbars are centered on the AQP4+/GFAP + lesion edge. Scale bars, 500 μm in C-F , 50 μm in G , H .

    Techniques Used: Activation Assay, Mouse Assay, Staining, Transgenic Assay

    9) Product Images from "Transient Outward K+ Current (Ito) Underlies the Right Ventricular Initiation of Polymorphic Ventricular Tachycardia in a Transgenic Rabbit Model of Long QT Type 1"

    Article Title: Transient Outward K+ Current (Ito) Underlies the Right Ventricular Initiation of Polymorphic Ventricular Tachycardia in a Transgenic Rabbit Model of Long QT Type 1

    Journal: Circulation. Arrhythmia and electrophysiology

    doi: 10.1161/CIRCEP.117.005414

    I to recovery from inactivation. A) The recovery kinetics was tested by a double-pulse protocol with interpulse time varying from 50 ms to 15 sec (n=12 RV and 7 LV cells from n=3 hearts). B) The amplitudes of the slow and fast inactivating components of I to (I to,si and I I to,fi ) as a function of inter-pulse interval were determined by fitting the time course of I to decay during the second pulse to a double exponential function. The x-axis of inter-pulse intervals is in a logarithmic scale. C) The amplitudes of I to,fi and I to,si from RV and LV. Fast and slow-inactivating components (I to,fi and I to,si ) of each I to,f and I to,s were calculated as described in Methods and represented as a stacked column plot. D) Western blots of Kv4.2, Kv1.4, and KChIP2 from LQT1 hearts. E). The accessory unit of I to , KChIP2, known to affect inactivation and recovery kinetics, was twofold higher in RV (ANOVA, p .
    Figure Legend Snippet: I to recovery from inactivation. A) The recovery kinetics was tested by a double-pulse protocol with interpulse time varying from 50 ms to 15 sec (n=12 RV and 7 LV cells from n=3 hearts). B) The amplitudes of the slow and fast inactivating components of I to (I to,si and I I to,fi ) as a function of inter-pulse interval were determined by fitting the time course of I to decay during the second pulse to a double exponential function. The x-axis of inter-pulse intervals is in a logarithmic scale. C) The amplitudes of I to,fi and I to,si from RV and LV. Fast and slow-inactivating components (I to,fi and I to,si ) of each I to,f and I to,s were calculated as described in Methods and represented as a stacked column plot. D) Western blots of Kv4.2, Kv1.4, and KChIP2 from LQT1 hearts. E). The accessory unit of I to , KChIP2, known to affect inactivation and recovery kinetics, was twofold higher in RV (ANOVA, p .

    Techniques Used: Mass Spectrometry, Size-exclusion Chromatography, Western Blot

    10) Product Images from "Bone marrow mesenchymal stem cells protected post-infarcted myocardium against arrhythmias via reversing potassium channels remodelling"

    Article Title: Bone marrow mesenchymal stem cells protected post-infarcted myocardium against arrhythmias via reversing potassium channels remodelling

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.12287

    Bone marrow mesenchymal stem cells (BMSCs) transplantation reversed the changes of K V 4.2, K V 4.3, K V 1.5 and K V 2.1 proteins in infarcted rats. Western blotting analysis of K V 4.2 ( A ), K V 4.3 ( B ), K V 1.5 ( C ), K V 1.4 ( D ) and K V 2.1 ( E ) proteins in LV myocytes of the border zone from control, infarcted and BMSCs-treated infarcted rats. ( F ) The bFGF level was elevated in infarcted myocardium with BMSCs transplantation. * P
    Figure Legend Snippet: Bone marrow mesenchymal stem cells (BMSCs) transplantation reversed the changes of K V 4.2, K V 4.3, K V 1.5 and K V 2.1 proteins in infarcted rats. Western blotting analysis of K V 4.2 ( A ), K V 4.3 ( B ), K V 1.5 ( C ), K V 1.4 ( D ) and K V 2.1 ( E ) proteins in LV myocytes of the border zone from control, infarcted and BMSCs-treated infarcted rats. ( F ) The bFGF level was elevated in infarcted myocardium with BMSCs transplantation. * P

    Techniques Used: Transplantation Assay, Western Blot

    11) Product Images from "Heteromultimeric Delayed-Rectifier K+ Channels in Schwann Cells: Developmental Expression and Role in Cell Proliferation"

    Article Title: Heteromultimeric Delayed-Rectifier K+ Channels in Schwann Cells: Developmental Expression and Role in Cell Proliferation

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.18-24-10398.1998

    Expression of Kv1.2 and Kv1.5 channel α subunits in mouse sciatic nerve during postnatal development. A , Left , Membrane fractions of sciatic nerves from P1, P4, P8, and P40 mice were subjected to SDS-PAGE and immunoblot analysis with anti-Kv1.5 antibodies. To estimate and compare total protein inputs in each lane, blots were stained with Ponceau S before immunoblot analysis (data not shown). Right , RT-PCR, followed by Southern blot analysis of Kv1.5 transcripts in sciatic nerves from P1 and P40 mice. B , Left , Immunoblot analysis of Kv1.2 on postnatal sciatic nerve as in A . Right , RT-PCR and Southern blot analysis of Kv1.2 as in A . Primer pairs to the specific 3′ coding regions of either Kv1.5 ( A ) or Kv1.2 ( B ) amplified PCR fragments of 273 and 248 bp, respectively. The bottom band represents the S16 ribosomal protein PCR fragment (102 bp), which was used to estimate the starting input RNA. C , Quantitation of the developmental downregulation of Kv1.2 and Kv1.5 proteins from P1 to P40 sciatic nerve as illustrated in A and B . Data of densitometric scanning were normalized to values of P40 and represent mean ± SEM of three independent experiments. * p
    Figure Legend Snippet: Expression of Kv1.2 and Kv1.5 channel α subunits in mouse sciatic nerve during postnatal development. A , Left , Membrane fractions of sciatic nerves from P1, P4, P8, and P40 mice were subjected to SDS-PAGE and immunoblot analysis with anti-Kv1.5 antibodies. To estimate and compare total protein inputs in each lane, blots were stained with Ponceau S before immunoblot analysis (data not shown). Right , RT-PCR, followed by Southern blot analysis of Kv1.5 transcripts in sciatic nerves from P1 and P40 mice. B , Left , Immunoblot analysis of Kv1.2 on postnatal sciatic nerve as in A . Right , RT-PCR and Southern blot analysis of Kv1.2 as in A . Primer pairs to the specific 3′ coding regions of either Kv1.5 ( A ) or Kv1.2 ( B ) amplified PCR fragments of 273 and 248 bp, respectively. The bottom band represents the S16 ribosomal protein PCR fragment (102 bp), which was used to estimate the starting input RNA. C , Quantitation of the developmental downregulation of Kv1.2 and Kv1.5 proteins from P1 to P40 sciatic nerve as illustrated in A and B . Data of densitometric scanning were normalized to values of P40 and represent mean ± SEM of three independent experiments. * p

    Techniques Used: Expressing, Mouse Assay, SDS Page, Staining, Reverse Transcription Polymerase Chain Reaction, Southern Blot, Amplification, Polymerase Chain Reaction, Quantitation Assay

    The heteromeric association of Kv1.2 and Kv1.5 channel α subunits in sciatic nerve. Reciprocal coimmunoprecipitation of Kv1.5 and Kv1.2 in P4 sciatic nerve is shown. A , Homogenates of acutely isolated sciatic nerves from P4 mice were subjected to immunoprecipitation with anti-Kv1.2, anti-Kv1.5, anti-Kv3.1, and preimmune antibodies. The Kv1.2 and Kv1.5 subunits were depleted from sciatic nerve extracts with their respective antibodies, and unbound proteins were subjected to a second round of immunoprecipitation with anti-Kv1.2 ( Kv1.5 depl. Kv1.2 ) and anti-Kv1.5 ( Kv1.2 depl. Kv1.5 ), respectively. Blots were probed with anti-Kv1.5. B , Reciprocal coimmunoprecipitation of Kv1.2 with Kv1.5 in P4 sciatic nerve. The blot shown in A was stripped and reprobed with anti-Kv1.2.
    Figure Legend Snippet: The heteromeric association of Kv1.2 and Kv1.5 channel α subunits in sciatic nerve. Reciprocal coimmunoprecipitation of Kv1.5 and Kv1.2 in P4 sciatic nerve is shown. A , Homogenates of acutely isolated sciatic nerves from P4 mice were subjected to immunoprecipitation with anti-Kv1.2, anti-Kv1.5, anti-Kv3.1, and preimmune antibodies. The Kv1.2 and Kv1.5 subunits were depleted from sciatic nerve extracts with their respective antibodies, and unbound proteins were subjected to a second round of immunoprecipitation with anti-Kv1.2 ( Kv1.5 depl. Kv1.2 ) and anti-Kv1.5 ( Kv1.2 depl. Kv1.5 ), respectively. Blots were probed with anti-Kv1.5. B , Reciprocal coimmunoprecipitation of Kv1.2 with Kv1.5 in P4 sciatic nerve. The blot shown in A was stripped and reprobed with anti-Kv1.2.

    Techniques Used: Isolation, Mouse Assay, Immunoprecipitation

    12) Product Images from "Species differences in the cerebellar distribution of six members of the Kv1 channel subfamily"

    Article Title: Species differences in the cerebellar distribution of six members of the Kv1 channel subfamily

    Journal: bioRxiv

    doi: 10.1101/2020.10.06.328237

    Cellular localizations of Kv1.4 subunit in the mouse (A, B); monkey (C, D) and human (E, F) cerebellar cortex. Immunohistochemistry was obtained using Alomone (A, C, E) and Santa Cruz (B, D, F) anti-Kv1.4 subunit antibodies. Strong Kv1.4 immunoreactivity was found in the Purkinje cell bodies in the mouse. In contrast, we observed no Purkinje cell immunoreactivity in monkey and human. In addition, in monkey and human tissue, using Alomone antibodies, for the first time, we report a strong immunoreactivity in cells in the molecular layer, which appear to be stellate in form (arrows). m: molecular layer; p: Purkinje cell layer; g: granular layer. Scale bar=50μm.
    Figure Legend Snippet: Cellular localizations of Kv1.4 subunit in the mouse (A, B); monkey (C, D) and human (E, F) cerebellar cortex. Immunohistochemistry was obtained using Alomone (A, C, E) and Santa Cruz (B, D, F) anti-Kv1.4 subunit antibodies. Strong Kv1.4 immunoreactivity was found in the Purkinje cell bodies in the mouse. In contrast, we observed no Purkinje cell immunoreactivity in monkey and human. In addition, in monkey and human tissue, using Alomone antibodies, for the first time, we report a strong immunoreactivity in cells in the molecular layer, which appear to be stellate in form (arrows). m: molecular layer; p: Purkinje cell layer; g: granular layer. Scale bar=50μm.

    Techniques Used: Immunohistochemistry

    Cellular localizations of Kv1.4 subunit in the mouse (A, B); monkey (C, D) and human (E, F) cerebellar nuclei. Immunohistochemistry was obtained using Alomone (A, C, E) and Santa Cruz (B, D, F) anti-Kv1.4 subunit antibodies. In mouse, generally strong Kv1.4 staining was detected in the cell bodies of cerebellar output neurons, using both sets of antibodies (A, B). In contrast, in primates, staining was absent from the soma of cerebellar output neurons (C, D, E, F). In addition, in primates, using Alomone antibodies, relatively strong (C, E) immunoreactivity was found in cells, which appear to be stellate in form. Scale bar=125μm.
    Figure Legend Snippet: Cellular localizations of Kv1.4 subunit in the mouse (A, B); monkey (C, D) and human (E, F) cerebellar nuclei. Immunohistochemistry was obtained using Alomone (A, C, E) and Santa Cruz (B, D, F) anti-Kv1.4 subunit antibodies. In mouse, generally strong Kv1.4 staining was detected in the cell bodies of cerebellar output neurons, using both sets of antibodies (A, B). In contrast, in primates, staining was absent from the soma of cerebellar output neurons (C, D, E, F). In addition, in primates, using Alomone antibodies, relatively strong (C, E) immunoreactivity was found in cells, which appear to be stellate in form. Scale bar=125μm.

    Techniques Used: Immunohistochemistry, Staining

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    Alomone Labs anti kv1 4 polyclonal antibodies
    Immunodetection of Kv1.2, <t>Kv1.4,</t> Kv1.5, and Kv1.6 channel proteins in OP cells/OLGs. A , B , Specificity of anti-Kv1.5 and anti-Kv1.6 <t>polyclonal</t> antibodies in transfected HEK 293 cells. Shown are Western blots of extracts from HEK 293 cells transfected
    Anti Kv1 4 Polyclonal Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv1 4 polyclonal antibodies/product/Alomone Labs
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti kv1 4 polyclonal antibodies - by Bioz Stars, 2022-09
    92/100 stars
      Buy from Supplier

    93
    Alomone Labs primary antibodies against kv1 3
    Effect of FS48 on the secretion of TNF-α and IL-2 in Jurkat T cells stimulated with PMA/ionomycin. A , effect of FS48 (1, 3, 10, 30 μM) and 100 nM MgTx on the proliferation of Jurkat T cells stimulated with PMA/ionomycin. B , knockdown of <t>Kv1.3</t> channel expression with different siRNA. C and D , the mRNA production of TNF-α and IL-2. E and F , the inhibition rate of TNF-α and IL-2 secretion in Jurkat T cells. G and H , the inhibition rate of TNF-α and IL-2 in Jurkat T cells after knockdown Kv1.3. All data are presented as mean ± SD (n ≥ 3). ### p
    Primary Antibodies Against Kv1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against kv1 3/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary antibodies against kv1 3 - by Bioz Stars, 2022-09
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    Immunodetection of Kv1.2, Kv1.4, Kv1.5, and Kv1.6 channel proteins in OP cells/OLGs. A , B , Specificity of anti-Kv1.5 and anti-Kv1.6 polyclonal antibodies in transfected HEK 293 cells. Shown are Western blots of extracts from HEK 293 cells transfected

    Journal: The Journal of Neuroscience

    Article Title: Characterization of Delayed Rectifier Kv Channels in Oligodendrocytes and Progenitor Cells

    doi: 10.1523/JNEUROSCI.17-21-08234.1997

    Figure Lengend Snippet: Immunodetection of Kv1.2, Kv1.4, Kv1.5, and Kv1.6 channel proteins in OP cells/OLGs. A , B , Specificity of anti-Kv1.5 and anti-Kv1.6 polyclonal antibodies in transfected HEK 293 cells. Shown are Western blots of extracts from HEK 293 cells transfected

    Article Snippet: Anti-Kv1.2 and anti-Kv1.4 polyclonal antibodies were purchased from Alomone Labs (Jerusalem, Israel), raised against GST-rat Kv channel fusion proteins (aa 417–498 and aa 589–655 for Kv1.2 and Kv1.4 channels, respectively), and affinity-purified.

    Techniques: Immunodetection, Transfection, Western Blot

    Effect of colchicine on the protein expression of calcium (Ca 2+ ) regulatory proteins and potassium channel proteins. ( A ) Representative immunoblotting and average data of sarcoplasmic reticulum Ca 2+ ATPase (SERCA2a), Cav1.2, Ca 2+ /calmodulin‐dependent protein kinase II (CaMKII), PLB, Ser16‐ and Thr17‐phosphorylated PLB (PLB‐Ser16 and PLB‐Thr17) from control and colchicine (3 nM)‐treated HL‐1 cells. ( n = 9) ( B ) Representative immunoblotting and average data of RyR type 2, phosphorylation of RyR at S2808 and S2814 (RyR‐2808 and RyR‐2814), the Na + –Ca 2+ exchanger (NCX), Kv1.4, Kv1.5 and Kv4.2 from control and colchicine (3 nM)‐treated HL‐1 cells ( n = 7). * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Colchicine modulates calcium homeostasis and electrical property of HL‐1 cells

    doi: 10.1111/jcmm.12818

    Figure Lengend Snippet: Effect of colchicine on the protein expression of calcium (Ca 2+ ) regulatory proteins and potassium channel proteins. ( A ) Representative immunoblotting and average data of sarcoplasmic reticulum Ca 2+ ATPase (SERCA2a), Cav1.2, Ca 2+ /calmodulin‐dependent protein kinase II (CaMKII), PLB, Ser16‐ and Thr17‐phosphorylated PLB (PLB‐Ser16 and PLB‐Thr17) from control and colchicine (3 nM)‐treated HL‐1 cells. ( n = 9) ( B ) Representative immunoblotting and average data of RyR type 2, phosphorylation of RyR at S2808 and S2814 (RyR‐2808 and RyR‐2814), the Na + –Ca 2+ exchanger (NCX), Kv1.4, Kv1.5 and Kv4.2 from control and colchicine (3 nM)‐treated HL‐1 cells ( n = 7). * P

    Article Snippet: Blots were probed with primary antibodies against ryanodine receptor type 2 (RyR; Affinity BioReagent, Golden, CO, USA), RyR phosphorylation at S2808 and S2814 (RyR‐2808 and RyR‐2814; Badrilla Leeds, UK), SR Ca2+ ATPase (SERCA2a, Santa Cruz Biotechnology, Santa Cruz, CA, USA), NCX (Swant, Bellinzona, Switzerland), Cav1.2 (ICa‐L subunit; Alomone Labs, Jerusalem, Israel), phospholamban (PLB; Thermo, Rockford, IL, USA), Thr17‐phosphorylated PLB (PLB‐Thr17; Badrilla, Leeds, UK), PLB‐Ser16 (Badrilla), Ca2+/calmodulin‐dependent protein kinase II (CaMKII, Abcam, Cambridge, UK), Kv1.4 (Alomone Labs), Kv1.5 (Alomone Labs), Kv4.2 (Abcam) and α‐actin (Sigma‐Aldrich, St. Louis, MO, USA).

    Techniques: Expressing

    Effect of FS48 on the secretion of TNF-α and IL-2 in Jurkat T cells stimulated with PMA/ionomycin. A , effect of FS48 (1, 3, 10, 30 μM) and 100 nM MgTx on the proliferation of Jurkat T cells stimulated with PMA/ionomycin. B , knockdown of Kv1.3 channel expression with different siRNA. C and D , the mRNA production of TNF-α and IL-2. E and F , the inhibition rate of TNF-α and IL-2 secretion in Jurkat T cells. G and H , the inhibition rate of TNF-α and IL-2 in Jurkat T cells after knockdown Kv1.3. All data are presented as mean ± SD (n ≥ 3). ### p

    Journal: The Journal of Biological Chemistry

    Article Title: The toxin mimic FS48 from the salivary gland of Xenopsylla cheopis functions as a Kv1.3 channel-blocking immunomodulator of T cell activation

    doi: 10.1016/j.jbc.2021.101497

    Figure Lengend Snippet: Effect of FS48 on the secretion of TNF-α and IL-2 in Jurkat T cells stimulated with PMA/ionomycin. A , effect of FS48 (1, 3, 10, 30 μM) and 100 nM MgTx on the proliferation of Jurkat T cells stimulated with PMA/ionomycin. B , knockdown of Kv1.3 channel expression with different siRNA. C and D , the mRNA production of TNF-α and IL-2. E and F , the inhibition rate of TNF-α and IL-2 secretion in Jurkat T cells. G and H , the inhibition rate of TNF-α and IL-2 in Jurkat T cells after knockdown Kv1.3. All data are presented as mean ± SD (n ≥ 3). ### p

    Article Snippet: Primary antibodies against Kv1.3 (4 °C, 16 h, 1: 200), p65, p38, p-p38, ERK, p-ERK, JNK, p-JNK, Histone H3, NF-кB p65, NFATc1, GAPDH, (4 °C, 16 h, 1:1000), and horseradish peroxidase–conjugated secondary antibodies (26 °C, 1 h, 1:2000) were applied, respectively.

    Techniques: Expressing, Inhibition

    Effects of FS48 on mRNA and protein expression of Kv1.3 channel. A , the viability of Jurkat T cells incubated with indicated concentrations of FS48 for 24 h. B , the relative expression analysis of KCNA3 mRNA in the presence and absence of FS48 and MgTx by qRT-PCR. C , Kv1.3 protein expression analysis of Kv1.3 channel. The cells were treated with PMA/ionomycin (50 ng/ml; 1 μg/ml) for 24 h after incubated with 3 μM FS48 and 100 nM MgTx for 1 h and then were collected for Western blot analysis. D , the ratios of Kv1.3 proteins to GAPDH. Quantity One software (Bio-Rad) was used for band density analysis. Data are shown as mean ± SD (n ≥ 3). # p

    Journal: The Journal of Biological Chemistry

    Article Title: The toxin mimic FS48 from the salivary gland of Xenopsylla cheopis functions as a Kv1.3 channel-blocking immunomodulator of T cell activation

    doi: 10.1016/j.jbc.2021.101497

    Figure Lengend Snippet: Effects of FS48 on mRNA and protein expression of Kv1.3 channel. A , the viability of Jurkat T cells incubated with indicated concentrations of FS48 for 24 h. B , the relative expression analysis of KCNA3 mRNA in the presence and absence of FS48 and MgTx by qRT-PCR. C , Kv1.3 protein expression analysis of Kv1.3 channel. The cells were treated with PMA/ionomycin (50 ng/ml; 1 μg/ml) for 24 h after incubated with 3 μM FS48 and 100 nM MgTx for 1 h and then were collected for Western blot analysis. D , the ratios of Kv1.3 proteins to GAPDH. Quantity One software (Bio-Rad) was used for band density analysis. Data are shown as mean ± SD (n ≥ 3). # p

    Article Snippet: Primary antibodies against Kv1.3 (4 °C, 16 h, 1: 200), p65, p38, p-p38, ERK, p-ERK, JNK, p-JNK, Histone H3, NF-кB p65, NFATc1, GAPDH, (4 °C, 16 h, 1:1000), and horseradish peroxidase–conjugated secondary antibodies (26 °C, 1 h, 1:2000) were applied, respectively.

    Techniques: Expressing, Incubation, Quantitative RT-PCR, Western Blot, Software

    Modulation of FS48 on endogenous voltage-gated potassium channels. A , representative traces of MgTx and different concentrations of FS48 suppressing the Kv1.3 currents in Jurkat T cells. Currents were elicited by applying 200 ms depolarization pulses from a holding potential of −70 mV to +40 mV in Jurkat T cells. B , concentration–response curve of FS48 inhibiting Kv1.3 currents in Jurkat T cells. Currents were normalized to the control and fitted by a Hill equation. C , current–voltage relationships (I-V). Test potentials were ranged from −50 mV to +40 mV with 10 mV increment steps. Y-axis represents the currents at different activation potential and normalized to the bath current at +40 mV in the present ( red ) or absent ( black ) of FS48; The solid lines represent the average Boltzmann sigmoidal fits. Data are shown as mean ± SD (n ≥ 3). ∗ p

    Journal: The Journal of Biological Chemistry

    Article Title: The toxin mimic FS48 from the salivary gland of Xenopsylla cheopis functions as a Kv1.3 channel-blocking immunomodulator of T cell activation

    doi: 10.1016/j.jbc.2021.101497

    Figure Lengend Snippet: Modulation of FS48 on endogenous voltage-gated potassium channels. A , representative traces of MgTx and different concentrations of FS48 suppressing the Kv1.3 currents in Jurkat T cells. Currents were elicited by applying 200 ms depolarization pulses from a holding potential of −70 mV to +40 mV in Jurkat T cells. B , concentration–response curve of FS48 inhibiting Kv1.3 currents in Jurkat T cells. Currents were normalized to the control and fitted by a Hill equation. C , current–voltage relationships (I-V). Test potentials were ranged from −50 mV to +40 mV with 10 mV increment steps. Y-axis represents the currents at different activation potential and normalized to the bath current at +40 mV in the present ( red ) or absent ( black ) of FS48; The solid lines represent the average Boltzmann sigmoidal fits. Data are shown as mean ± SD (n ≥ 3). ∗ p

    Article Snippet: Primary antibodies against Kv1.3 (4 °C, 16 h, 1: 200), p65, p38, p-p38, ERK, p-ERK, JNK, p-JNK, Histone H3, NF-кB p65, NFATc1, GAPDH, (4 °C, 16 h, 1:1000), and horseradish peroxidase–conjugated secondary antibodies (26 °C, 1 h, 1:2000) were applied, respectively.

    Techniques: Concentration Assay, Activation Assay