Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Inward Rectifier K + Currents Contribute to the Proarrhythmic Electrical Phenotype of Atria Overexpressing Cyclic Adenosine Monophosphate Response Element Modulator Isoform CREM‐IbΔC‐X

doi: 10.1161/JAHA.119.016144

Figure Lengend Snippet: Experimental animals involved wild‐type mice (WT) and transgenic mice (TG) expressing the repressor isoform of cyclic adenosine monophosphate response element modulator, CREM‐IbΔC‐X, and were distributed according to age in 6‐week‐old (WT 6w and TG 6w ) and 12‐week‐old (WT 12w and TG 12w ) groups. A , Representative transient outward K + current (I to ) traces recorded as −40 mV sensitive currents from atrial cardiomyocytes of WT 12w and TG 12w . For clarity, current density (expressed in picoamperes/picofarads [pA/pF]) traces from −40 mV with +20 mV increments are displayed. B , Mean±SE of current–voltage plots of the peak current density (I to ). For WT 6w , n/N are 41/15, for TG 6w n/N are 22/14, for WT 12w n/N are 35/19, and for TG 12w n/N are 20/16. Ca through d , Representative immunoblots and quantification of protein expression of calsequestrin (CSQ; Ca ) normalized to Ponceau and of K + voltage‐gated channel subfamily D member 2 (Kv4.2, Cb ) and 3 (Kv4.3, Cc ), and K + channel interacting protein 2 (KChIP2) ( Cd ) normalized to CSQ. Data show mean±SE of N=7 mice per group, except for Kv4.3, where N=6 as the result of an undetectable band in TG 12w . * P <0.05 TG 6w vs WT 6w (gray), TG 12w vs WT 12w (black), # P <0.05 WT 12w vs WT 6w (gray), TG 12w vs TG 6w (black) from 2‐way repeated‐measures analysis of variance with Bonferroni post hoc test ( B ) and parametric or nonparametric 1‐way analysis of variance with the corresponding post hoc test ( C ).

Article Snippet: The following rabbit polyclonal primary antibodies were used against K + voltage‐gated channels (Kv) subunits Kv4.2 (1:200, APC 023, Alomone Labs, Jerusalem, Israel), Kv4.3 (1:200, APC 017, Alomone Labs), K + channel interacting protein 2 (KChIP2) (1:200, sc‐25685, Santa Cruz Biotechnology Inc, Santa Cruz, CA), and K + inwardly rectifying channel (Kir) subunits Kir2.1 (1:200, APC 159, Alomone Labs), Kir2.3 (1:1000, APC 032, Alomone Labs), Kir3.1 (1:200, APC 005, Alomone Labs), Kir3.4 (1:200, APC 027, Alomone Labs), and as loading control calsequestrin (1:2500, PA1‐913, Thermo Fisher Scientific).

Techniques: Transgenic Assay, Expressing, Western Blot

Journal:

Article Title: Both Laminin and Schwann cell Dystroglycan are necessary for proper clustering of Sodium Channels at Nodes of Ranvier.

doi: 10.1523/JNEUROSCI.2068-05.2005

Figure Lengend Snippet: Antibodies

Article Snippet: Potassium channel Kv 1.1 , pAb , rabbit , , 1:100 , Alomone Labs.

Techniques: