ksr xf (Thermo Fisher)
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Name:
CTS KnockOut SR XenoFree Medium
Description:
GIBCO CTS KnockOut SR XenoFree Medium when formulated as a complete medium enables the growth and expansion of human embryonic stem cells hESC and human induced pluripotent stem cells hiPSC in a cell culture medium containing only human derived or human recombinant proteins to facilitate the transition of hESC and hiPSC research from the bench to the clinic The CTS product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications • Defined xenofree medium for hESC⁄iPSC stem cell culture • First to market xenofree hESC⁄iPSC product intended for Ex Vivo tissue and cell culture processing applications CTS KnockOut SR XenoFree Medium does not contain bovine or other non human animal derived components Besides pluripotent stem cell culture expansion and maintenance CTS KnockOut SR XenoFree Medium can be used for hESC⁄hiPSC cryopreservation derivation and differentiation studies GIBCO CTS products are of high quality and are supplied with harmonized documentation such as Certificates of Analysis Certificates of Origin and access to authorization letters for our Drug Master Files as appropriate For human ex vivo tissue and cell culture processing applications CAUTION Not intended for direct administration to humans or animals
Catalog Number:
12618013
Price:
None
Category:
Cell Culture Transfection Reagents
Applications:
Cell Culture|Cell Therapy Systems|Clinical|Clinical & Translational Research|Embryonic Stem Cell Culture|Induced Pluripotent Stem Cell Culture|Mammalian Cell Culture|Stem Cell Culture|Stem Cell Research|Stem Cell Therapy Research
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Structured Review
Thermo Fisher
ksr xf
GIBCO CTS KnockOut SR XenoFree Medium when formulated as a complete medium enables the growth and expansion of human embryonic stem cells hESC and human induced pluripotent stem cells hiPSC in a cell culture medium containing only human derived or human recombinant proteins to facilitate the transition of hESC and hiPSC research from the bench to the clinic The CTS product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications • Defined xenofree medium for hESC⁄iPSC stem cell culture • First to market xenofree hESC⁄iPSC product intended for Ex Vivo tissue and cell culture processing applications CTS KnockOut SR XenoFree Medium does not contain bovine or other non human animal derived components Besides pluripotent stem cell culture expansion and maintenance CTS KnockOut SR XenoFree Medium can be used for hESC⁄hiPSC cryopreservation derivation and differentiation studies GIBCO CTS products are of high quality and are supplied with harmonized documentation such as Certificates of Analysis Certificates of Origin and access to authorization letters for our Drug Master Files as appropriate For human ex vivo tissue and cell culture processing applications CAUTION Not intended for direct administration to humans or animals
https://www.bioz.com/result/ksr xf/product/Thermo Fisher
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
GIBCO CTS KnockOut SR XenoFree Medium when formulated as a complete medium enables the growth and expansion of human embryonic stem cells hESC and human induced pluripotent stem cells hiPSC in a cell culture medium containing only human derived or human recombinant proteins to facilitate the transition of hESC and hiPSC research from the bench to the clinic The CTS product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications • Defined xenofree medium for hESC⁄iPSC stem cell culture • First to market xenofree hESC⁄iPSC product intended for Ex Vivo tissue and cell culture processing applications CTS KnockOut SR XenoFree Medium does not contain bovine or other non human animal derived components Besides pluripotent stem cell culture expansion and maintenance CTS KnockOut SR XenoFree Medium can be used for hESC⁄hiPSC cryopreservation derivation and differentiation studies GIBCO CTS products are of high quality and are supplied with harmonized documentation such as Certificates of Analysis Certificates of Origin and access to authorization letters for our Drug Master Files as appropriate For human ex vivo tissue and cell culture processing applications CAUTION Not intended for direct administration to humans or animals
https://www.bioz.com/result/ksr xf/product/Thermo Fisher
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ksr xf - by Bioz Stars,
2021-03
94/100 stars
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Related Articles
Knock-Out:Article Title: Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method Article Snippet: .. Undifferentiated hPSCs were detached and transferred to Corning® Costar® Ultra-Low attachment plates in XF-Ko-SR medium (KnockOut™ Dulbecoo’s modified Eagle’s medium (DMEM) supplemented with 15% Article Title: Evaluation of Therapeutic Oligonucleotides for Familial Amyloid Polyneuropathy in Patient-Derived Hepatocyte-Like Cells Article Snippet: After 24 hours, culture medium was changed for the next 3 days to DMEM/F12 supplemented with 100 ng/ml recombinant Activin-A (R & D Systems), 100 ng/ml fibroblast growth factor-2 (FGF2, Peprotech), 50 ng/ml recombinant human Wnt3a (R & D Systems). .. Concentration of Article Title: Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells Article Snippet: At the end of Stage 3, cells were detached and either re-plated at a 30%–40% confluence in 3D sandwich culture or seeded into decellularized liver matrix for further maturation. .. Cells were grown for 4 days in a defined medium containing 45% DMEM low glucose 1g/l (ThermoFisher Scientific, Waltham, MA), 45% F-12 (ThermoFisher Scientific, Waltham, MA), 10% Article Title: A short-term plastic adherence incubation of the stromal vascular fraction leads to a predictable GMP-compliant cell-product Article Snippet: The resulting cells were washed twice with PBS w/o Mg2+ Ca2+ (Biochrom, Berlin, Germany), 2% KnockOut™ (ThermoFisher Scientific, Waltham, USA). .. For short-term incubation and purification, the cells were seeded on cell culture dishes (Greiner Bio-One) at high density (≥ 1.0 x 105 cells/cm2 ) in DMEM-F12, 2% Article Title: A short-term plastic adherence incubation of the stromal vascular fraction leads to a predictable GMP-compliant cell-product Article Snippet: The digestion was stopped with EDTA (Corning Inc., New York , USA) as recommended by Nordmark for Collagenase digestion. .. The cell pellet was resuspended in DMEM-F12, 2% Modification:Article Title: Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method Article Snippet: .. Undifferentiated hPSCs were detached and transferred to Corning® Costar® Ultra-Low attachment plates in XF-Ko-SR medium (KnockOut™ Dulbecoo’s modified Eagle’s medium (DMEM) supplemented with 15% Staining:Article Title: Optimized Approaches for the Induction of Putative Canine Induced Pluripotent Stem Cells from Old Fibroblasts Using Synthetic RNAs Article Snippet: Putative ciPSC colonies were cultured in stage 3 medium containing 10 ng/mL of bFGF (Bio-Bud) or 1000 units/mL of murine leukemia inhibitory factor (mLIF; ESGRO, Millipore, Billerica, MA, USA), plus 0.5 μM of MEK1/2 inhibitor (PD0325901; Stemgent, Cambridge, MA, USA) and 3 μM of GSK3β inhibitor (CHIR99021; Stemgent). .. Primary iPSC Colony Staining with Alkaline Phosphatase (AP) and TRA-1-60ciPSCs were washed with PBS three times after removing the Concentration Assay:Article Title: Evaluation of Therapeutic Oligonucleotides for Familial Amyloid Polyneuropathy in Patient-Derived Hepatocyte-Like Cells Article Snippet: After 24 hours, culture medium was changed for the next 3 days to DMEM/F12 supplemented with 100 ng/ml recombinant Activin-A (R & D Systems), 100 ng/ml fibroblast growth factor-2 (FGF2, Peprotech), 50 ng/ml recombinant human Wnt3a (R & D Systems). .. Concentration of Incubation:Article Title: A short-term plastic adherence incubation of the stromal vascular fraction leads to a predictable GMP-compliant cell-product Article Snippet: The resulting cells were washed twice with PBS w/o Mg2+ Ca2+ (Biochrom, Berlin, Germany), 2% KnockOut™ (ThermoFisher Scientific, Waltham, USA). .. For short-term incubation and purification, the cells were seeded on cell culture dishes (Greiner Bio-One) at high density (≥ 1.0 x 105 cells/cm2 ) in DMEM-F12, 2% Purification:Article Title: A short-term plastic adherence incubation of the stromal vascular fraction leads to a predictable GMP-compliant cell-product Article Snippet: The resulting cells were washed twice with PBS w/o Mg2+ Ca2+ (Biochrom, Berlin, Germany), 2% KnockOut™ (ThermoFisher Scientific, Waltham, USA). .. For short-term incubation and purification, the cells were seeded on cell culture dishes (Greiner Bio-One) at high density (≥ 1.0 x 105 cells/cm2 ) in DMEM-F12, 2% Cell Culture:Article Title: A short-term plastic adherence incubation of the stromal vascular fraction leads to a predictable GMP-compliant cell-product Article Snippet: The resulting cells were washed twice with PBS w/o Mg2+ Ca2+ (Biochrom, Berlin, Germany), 2% KnockOut™ (ThermoFisher Scientific, Waltham, USA). .. For short-term incubation and purification, the cells were seeded on cell culture dishes (Greiner Bio-One) at high density (≥ 1.0 x 105 cells/cm2 ) in DMEM-F12, 2% Recombinant:Article Title: CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice Article Snippet: Human embryonic and neural stem cell culture and differentiation Culture of hESC lines Shef3, Shef4, and Shef6 (University of Sheffield, UK) was established at UC Irvine in accordance with all appropriate hSCRO and IBC protocols on mitotically-inactivated mouse embryonic fibroblasts (MEFs, EMD Millipore) and in defined media consisting of KO DMEM/ F12, 20% KO Serum Replacement (KO SR), 0.1 mM NEAA, 2 mM GlutaMAX, 0.1 mM β-Mercaptoethanol, and 20 ng/mL bFGF (All from Life Technologies). .. To transition cells to Xeno-Free (XF) culture conditions, all non-human animal-based components (MEFs, KOSR) were removed and replaced with human-based or recombinant alternatives including CELLstart CTS, |
![Stage 3: Generation of a homogenous and functional retinal pigment epithelium (RPE) population. (A): Two days’ postseeding, SHEF1 human embryonic stem cells were treated with Induction Medium 1 for 4 days, followed by Induction Medium 2 for 3 days. At day 9, cells were replated in Induction Medium 3 for a period of 19 days. At day 28, cells were replated in Dulbecco's modified Eagle's medium KSR‐XF and cultured for a period of 14 days. Representative images showing immunocytochemistry for indicated RPE markers are shown. Images are captured at ×10 magnification. (B): Cells cultured as in panel A were further dissociated and replated on transwells and cultured for a period of 10 weeks. A representative image showing en face immunocytochemistry for ZO‐1 is shown. Images are captured at ×20 magnification. (C): Spent medium from transwells described in panel B was collected from the top and bottom chambers and quantified for vascular endothelial growth factor (VEGF) and pigment epithelium‐derived factor (PEDF) concentration. The ratio of [VEGF]:[PEDF] was quantified in media from the two compartments ( n = 3, ±SD). (D): Representative confocal images showing phagocytosis of fluorescent bead (red) by RPE. ZO‐1 immunocytochemistry (green) shows the cell edge, and the presence of bead within the cell boundary indicates internalization by phagocytosis. Images are captured at ×63 magnification. Abbreviations: PEDF, pigment epithelium‐derived factor; VEGF, vascular endothelial growth factor.](https://storage.googleapis.com/bioz_article_images/PMC5442825/SCT3-6-490-g004.jpg)