Journal: bioRxiv

Article Title: Olfactomedin4 marks luminal progenitor cells that give rise to secretory cell lineage in the mouse cervix

doi: 10.64898/2026.04.28.721179

Figure Lengend Snippet: A. Schematic of lineage-tracing strategy using Krt8-Cre ERT2 ; Rosa26 tdTomato mice with tissues collected across nonpregnant and early-to late-pregnancy: gestation day (GD) 6, 12, 15, 18 and in labor (IL). B. RNA expression in the endocervix for tdTomato (red), Spdef (yellow), and Muc5b (pink) across diestrus, pregnancy stages and in labor. Spdef and Muc5b were co-stained in the same section, with each marker displayed in separate panels, whereas tdTomato was detected on a separate section. Epi-epithelial and Str- stromal regions are indicated. Images are representative of n=1 biological replicate per time point and scale bars =100 µm.

Article Snippet: The Krt8-Cre/ ERT2 (17Blpn/J) mice were sourced from Jackson Laboratories.

Techniques: RNA Expression, Staining, Marker

Journal: bioRxiv

Article Title: Olfactomedin4 marks luminal progenitor cells that give rise to secretory cell lineage in the mouse cervix

doi: 10.64898/2026.04.28.721179

Figure Lengend Snippet: A. Schematic of lineage tracing strategy using Olfm4-Cre ERT2 ; Rosa26 tdTomato mice. B. RNA expression of tdTomato+ (red), Spdef (cyan) and Muc5b (green) in endocervical tissue from nonpregnant (diestrus) and pregnancy (GD 6,12,15, 18 and IL). All three markers were detected in the same section, with each signal displayed in separate panels. Images are representative of n≥ 3 biological replicates per time point and scale bars =100 µm. C. Schematic of workflow used to generate organoid cultures from tdTomato negative and tdTomato positive epithelial populations from the cervix of nonpregnant diestrus Olfm4-Cre ERT2 ; Rosa26 tdTomato mice. D. Organoids generated from flow cytometry sorted tdTomato+ and tdTomato- epithelial populations. tdTomato+ derived organoids retained tdTomato signal, whereas tdTomato-progenitors formed organoids that did not express tdTomato. n = 3 biological replicates and scale bars =100 µm. E. Schematic of short-term and long-term lineage-tracing strategy using Olfm4 Cre ERT2 ; Rosa26 tdTomato mice (8-week-old females). Short-term tracing (top): Tamoxifen (Tmx) was administered at estrus, diestrus, or GD11, and tissues were collected 24-48 hrs later at the subsequent time points (estrus to diestrus, diestrus to estrus, GD11 to GD12). Long-term tracing (bottom): Tmx was administered at the NP stage, followed by a first pregnancy. After the first pregnancy, tissues were collected at two stages of the estrous cycle, or on GD15 of a second pregnancy. F. RNAscope images of endocervix from short-term tracing experiments. Panels indicate tdTomato⁺ (red), Spdef (cyan), Muc5b (green) and nuclei (blue). In short-term tracing, tdTomato⁺ cells are present and co-localize with goblet markers during diestrus (first column) and on GD12 (third column). All three markers were stained in the same section and are displayed in separate panels. Representative images from n = 2 biological replicates per condition. Scale bars = 100 µm. G. RNAscope images of endocervix from long-term tracing experiments. Panels indicate tdTomato⁺ (red), Spdef (cyan), Muc5b (green) and nuclei (blue). In long-term tracing, tdTomato⁺ cells persist and give rise to secretory goblet cells that express Spdef and Muc5b in diestrus (second column) and in a second pregnancy at GD15 (third column). All three markers were stained in the same section and are displayed in separate panels. Representative images from n = 2 biological replicates per condition. Scale bars = 100 µm.

Article Snippet: The Krt8-Cre/ ERT2 (17Blpn/J) mice were sourced from Jackson Laboratories.

Techniques: RNA Expression, Generated, Flow Cytometry, Derivative Assay, RNAscope, Staining

Journal: Translational Oncology

Article Title: UCHL1 promotes temozolomide resistance in glioblastoma by inhibiting the ubiquitination-mediated degradation of keratin 8

doi: 10.1016/j.tranon.2026.102728

Figure Lengend Snippet: UCHL1 induces TMZ resistance by deubiquitination-mediated stabilization of KRT8 in glioblastoma cells. (A) We identified chemoresistant and anti-apoptotic genes in the database, as revealed by IP-MS. Western blotting analysis shows KRT8 levels in glioblastoma cells subjected to UCHL1 knockdown (B) and UCHL1 overexpression (C). (D) The Co-IP assay indicates the interaction between UCHL1 and KRT8 in U87MG cells. (E) HEK293T cells were co-transfected with the indicated plasmids, harvested 48 h post-transfection, and the ubiquitination level of Myc‑tagged KRT8 was determined. (F) HEK293T cells were co-transfected with the indicated plasmids, harvested 48 h post-transfection, and subjected to ubiquitination assay to specifically assess the contribution of K27‑linked ubiquitin chains to KRT8 ubiquitination. * p < 0.05.

Article Snippet: KRT8 siRNA and negative control siRNA were purchased from Sangon Biotech (Shanghai, China).

Techniques: Protein-Protein interactions, Western Blot, Knockdown, Over Expression, Co-Immunoprecipitation Assay, Transfection, Ubiquitin Proteomics

Journal: Translational Oncology

Article Title: UCHL1 promotes temozolomide resistance in glioblastoma by inhibiting the ubiquitination-mediated degradation of keratin 8

doi: 10.1016/j.tranon.2026.102728

Figure Lengend Snippet: KRT8 is a key protein in UCHL1-mediated TMZ resistance in glioblastoma. (A) Survival probability was assessed based on KRT8 expression levels in primary and recurrent glioma samples using data from the CGGA dataset. (B) KRT8 expression levels in WHO grade II, III, and IV glioma tissues, as derived from the CGGA dataset. (C) Western blotting analysis depicting KRT8 expression in U251-sh UCHL1 cells following KRT8 overexpression. (D) Cell viability curves for KRT8 overexpression in U251-sh UCHL1 cells subjected to TMZ treatment. (E) Survival analysis based on UCHL1 and KRT8 co‑expression in glioblastoma patients (TCGA dataset). *** p < 0.001.

Article Snippet: KRT8 siRNA and negative control siRNA were purchased from Sangon Biotech (Shanghai, China).

Techniques: Expressing, Derivative Assay, Western Blot, Over Expression

Journal: Cell reports

Article Title: Aging disrupts sympathetic innervation of the thymus

doi: 10.1016/j.celrep.2026.117126

Figure Lengend Snippet: (A) Representative confocal z stack projection of a 50 μm cryosection demonstrating the loss of β3-tubulin + innervation of large blood vessels immediately before entering the thymic medulla (white arrowheads). The abrupt ending of nerves occurred at the same location as a reduction in α-SMA + cells, and the medulla contains non-vascular α-SMA + cells (green arrowhead). (B) TH + sympathetic innervation ends (yellow arrowhead) along a blood vessel that projects from the cortex, labeled with the cTEC marker keratin 8, into the medulla, labeled with the mTEC marker keratin 5. (C and D) TH + /β3-tubulin + sympathetic nerves innervating a large vessel along the cortico-medullary junction, with cortex/medulla denoted by cell density (Hoechst, C) and mTEC marker (keratin 8, D). Representative confocal z stack projection of TH + sympathetic innervation ending along a blood vessel that enters the medulla (blue arrowheads). Dashed white lines represent cortico-medullary junctions. (E and F) Quantification of TH + segments per thymic region for all sections (E) and average per mouse (F); n = 3–4 sections per mouse for 4 mice. Data are presented as mean ± standard error of the mean and analyzed with repeated measures one-way ANOVA with Tukey’s multiple comparisons test. Subcap, subcapsular cortex; Ctx, cortex only; Med, medulla only; CMJ, cortico-medullary junction; and Ctx and Med, both cortex and medullary regions. Scale bars, 100 μm in (A)–(D).

Article Snippet: Cytokeratin 8 (KRT8) Guinea Pig Polyclonal Antibody , Acris Antibodies , Cat#BP5075; RRID: AB_979823.

Techniques: Labeling, Marker