kpni  (New England Biolabs)


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    Structured Review

    New England Biolabs kpni
    Kpni, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kpni/product/New England Biolabs
    Average 99 stars, based on 174 article reviews
    Price from $9.99 to $1999.99
    kpni - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Countercurrent Chromatography:

    Article Title: Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization
    Article Snippet: HTLV-1 SL(450–470) (5′-AUG GGC CAA AUC UUU UCC CGU UU -3′) and SL(474–508) (5′-GCU AGC CCU AUU CCG CGG CCG CCC CGG GGG CUG GC U U -3′) were purchased from Dharmacon (GE healthcare). .. The following HTLV-1 gRNA constructs were derived from the proviral plasmid ACHneo ( ) (a gift from Patrick L. Green, Department of Veterinary Biosciences, The Ohio State University) and cloned into KpnI and PstI sites of pUC19 (New England Biolabs) in front of a T7 RNA polymerase promoter: HTLV-1 SL(447–512) derived from nt 447–512, R derived from nt 1–228, U5 derived from nt 229–450, 5′ gag derived from 450–625, and 5′ leader derived from nt 1–625.

    Clone Assay:

    Article Title: Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization
    Article Snippet: .. The following HTLV-1 gRNA constructs were derived from the proviral plasmid ACHneo ( ) (a gift from Patrick L. Green, Department of Veterinary Biosciences, The Ohio State University) and cloned into KpnI and PstI sites of pUC19 (New England Biolabs) in front of a T7 RNA polymerase promoter: HTLV-1 SL(447–512) derived from nt 447–512, R derived from nt 1–228, U5 derived from nt 229–450, 5′ gag derived from 450–625, and 5′ leader derived from nt 1–625. .. A 15-nt 5′-extension (GGC CTT CGG GCC AAG) was added to these gRNA constructs to facilitate SHAPE analysis ( ).

    In Vitro:

    Article Title: Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization
    Article Snippet: All other HTLV-1 gRNA-derived constructs were in vitro transcribed from digested plasmids using T7 RNA polymerase and previously established methods ( ). .. The following HTLV-1 gRNA constructs were derived from the proviral plasmid ACHneo ( ) (a gift from Patrick L. Green, Department of Veterinary Biosciences, The Ohio State University) and cloned into KpnI and PstI sites of pUC19 (New England Biolabs) in front of a T7 RNA polymerase promoter: HTLV-1 SL(447–512) derived from nt 447–512, R derived from nt 1–228, U5 derived from nt 229–450, 5′ gag derived from 450–625, and 5′ leader derived from nt 1–625.

    Southern Blot:

    Article Title: Generation and characterization of bat-induced pluripotent stem cells.
    Article Snippet: Paragraph title: Southern blot analysis and PCR ... The genomic DNAs were digested with KpnI (NEB).

    Synthesized:

    Article Title: Generation and characterization of bat-induced pluripotent stem cells.
    Article Snippet: The genomic DNAs were digested with KpnI (NEB). .. The probe was synthesized by PCR with DIG-labeled dNTPs.

    Mutagenesis:

    Article Title: Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization
    Article Snippet: The following HTLV-1 gRNA constructs were derived from the proviral plasmid ACHneo ( ) (a gift from Patrick L. Green, Department of Veterinary Biosciences, The Ohio State University) and cloned into KpnI and PstI sites of pUC19 (New England Biolabs) in front of a T7 RNA polymerase promoter: HTLV-1 SL(447–512) derived from nt 447–512, R derived from nt 1–228, U5 derived from nt 229–450, 5′ gag derived from 450–625, and 5′ leader derived from nt 1–625. .. To probe potential dimerization sites in the 5′ leader, three palindromic loop regions, nt 348–350, nt 382–386, and nt 498–500, were mutated to GAGA tetraloops individually and simultaneously using site-directed, ligase-independent mutagenesis (SLIM) ( ).

    Labeling:

    Article Title: Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization
    Article Snippet: The two Us in italics are not encoded by HTLV-1 and were added to facilitate fluorescent labeling and ensure free rotation of the dye. .. The following HTLV-1 gRNA constructs were derived from the proviral plasmid ACHneo ( ) (a gift from Patrick L. Green, Department of Veterinary Biosciences, The Ohio State University) and cloned into KpnI and PstI sites of pUC19 (New England Biolabs) in front of a T7 RNA polymerase promoter: HTLV-1 SL(447–512) derived from nt 447–512, R derived from nt 1–228, U5 derived from nt 229–450, 5′ gag derived from 450–625, and 5′ leader derived from nt 1–625.

    Purification:

    Article Title: Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization
    Article Snippet: The purified protein was stored at −20 °C. .. The following HTLV-1 gRNA constructs were derived from the proviral plasmid ACHneo ( ) (a gift from Patrick L. Green, Department of Veterinary Biosciences, The Ohio State University) and cloned into KpnI and PstI sites of pUC19 (New England Biolabs) in front of a T7 RNA polymerase promoter: HTLV-1 SL(447–512) derived from nt 447–512, R derived from nt 1–228, U5 derived from nt 229–450, 5′ gag derived from 450–625, and 5′ leader derived from nt 1–625.

    Plasmid Preparation:

    Article Title: Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization
    Article Snippet: .. The following HTLV-1 gRNA constructs were derived from the proviral plasmid ACHneo ( ) (a gift from Patrick L. Green, Department of Veterinary Biosciences, The Ohio State University) and cloned into KpnI and PstI sites of pUC19 (New England Biolabs) in front of a T7 RNA polymerase promoter: HTLV-1 SL(447–512) derived from nt 447–512, R derived from nt 1–228, U5 derived from nt 229–450, 5′ gag derived from 450–625, and 5′ leader derived from nt 1–625. .. A 15-nt 5′-extension (GGC CTT CGG GCC AAG) was added to these gRNA constructs to facilitate SHAPE analysis ( ).

    Article Title: Generation and characterization of bat-induced pluripotent stem cells.
    Article Snippet: The genomic DNAs were digested with KpnI (NEB). .. Southern blot analysis and PCR The colonies that were detected as negative in Southern blots were further confirmed by PCR with GoTaq polymerase using primers specific for the Neo gene of the pSTEM-h103 vector, as it is more sensitive than the Southern blot.

    Construct:

    Article Title: Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization
    Article Snippet: .. The following HTLV-1 gRNA constructs were derived from the proviral plasmid ACHneo ( ) (a gift from Patrick L. Green, Department of Veterinary Biosciences, The Ohio State University) and cloned into KpnI and PstI sites of pUC19 (New England Biolabs) in front of a T7 RNA polymerase promoter: HTLV-1 SL(447–512) derived from nt 447–512, R derived from nt 1–228, U5 derived from nt 229–450, 5′ gag derived from 450–625, and 5′ leader derived from nt 1–625. .. A 15-nt 5′-extension (GGC CTT CGG GCC AAG) was added to these gRNA constructs to facilitate SHAPE analysis ( ).

    Polymerase Chain Reaction:

    Article Title: Generation and characterization of bat-induced pluripotent stem cells.
    Article Snippet: Paragraph title: Southern blot analysis and PCR ... The genomic DNAs were digested with KpnI (NEB).

    Cellular Antioxidant Activity Assay:

    Article Title: Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization
    Article Snippet: HTLV-1 SL(450–470) (5′-AUG GGC CAA AUC UUU UCC CGU UU -3′) and SL(474–508) (5′-GCU AGC CCU AUU CCG CGG CCG CCC CGG GGG CUG GC U U -3′) were purchased from Dharmacon (GE healthcare). .. The following HTLV-1 gRNA constructs were derived from the proviral plasmid ACHneo ( ) (a gift from Patrick L. Green, Department of Veterinary Biosciences, The Ohio State University) and cloned into KpnI and PstI sites of pUC19 (New England Biolabs) in front of a T7 RNA polymerase promoter: HTLV-1 SL(447–512) derived from nt 447–512, R derived from nt 1–228, U5 derived from nt 229–450, 5′ gag derived from 450–625, and 5′ leader derived from nt 1–625.

    Derivative Assay:

    Article Title: Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization
    Article Snippet: .. The following HTLV-1 gRNA constructs were derived from the proviral plasmid ACHneo ( ) (a gift from Patrick L. Green, Department of Veterinary Biosciences, The Ohio State University) and cloned into KpnI and PstI sites of pUC19 (New England Biolabs) in front of a T7 RNA polymerase promoter: HTLV-1 SL(447–512) derived from nt 447–512, R derived from nt 1–228, U5 derived from nt 229–450, 5′ gag derived from 450–625, and 5′ leader derived from nt 1–625. .. A 15-nt 5′-extension (GGC CTT CGG GCC AAG) was added to these gRNA constructs to facilitate SHAPE analysis ( ).

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    New England Biolabs kpn i
    Southern blot and PCR analysis for wt AAV <t>DNA</t> at the site of administration. (A) Southern blot of unamplified, Kpn I-digested genomic DNA isolated from the site of wt AAV administration. Liver tissue from animals infected intravenously (lanes 1 and 2), muscle tissue from animals infected intramuscularly (lanes 4 to 6), and nasal tissue from animals infected intranasally either without (lanes 8 and 9) or with (lanes 10 and 11) helper Ad were examined. Liver, muscle, and nasal epithelial DNAs from a saline-injected control animal were also examined (lanes 3, 7, and 12, respectively). Plasmid DNAs equating to 0.1, 1, 10, and 100 copies of wt AAV genomes per cell were included as standards (lanes 13 to 16, respectively). (B) PCR analysis for internal AAV-Rep gene sequences was performed for each of the same samples.
    Kpn I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kpn i/product/New England Biolabs
    Average 99 stars, based on 196 article reviews
    Price from $9.99 to $1999.99
    kpn i - by Bioz Stars, 2020-04
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    95
    New England Biolabs ecori kpni
    Genetic and restriction map of the constructed plasmids. A ( a ) pCMV6-XL5-SMN digested with NotI, ( b ) pAAV-CB6-PI was digested with <t>EcoRI</t> and <t>KpnI,</t> to construct ( c ) pAAVsc-CB6-PI- SMN. B ( d ) pCMV6-XL5-SMN was digested with Bgl II, ( e ) pAAVsc-CB6-PI was
    Ecori Kpni, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori kpni/product/New England Biolabs
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ecori kpni - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    Southern blot and PCR analysis for wt AAV DNA at the site of administration. (A) Southern blot of unamplified, Kpn I-digested genomic DNA isolated from the site of wt AAV administration. Liver tissue from animals infected intravenously (lanes 1 and 2), muscle tissue from animals infected intramuscularly (lanes 4 to 6), and nasal tissue from animals infected intranasally either without (lanes 8 and 9) or with (lanes 10 and 11) helper Ad were examined. Liver, muscle, and nasal epithelial DNAs from a saline-injected control animal were also examined (lanes 3, 7, and 12, respectively). Plasmid DNAs equating to 0.1, 1, 10, and 100 copies of wt AAV genomes per cell were included as standards (lanes 13 to 16, respectively). (B) PCR analysis for internal AAV-Rep gene sequences was performed for each of the same samples.

    Journal: Journal of Virology

    Article Title: Latent Adeno-Associated Virus Infection Elicits Humoral but Not Cell-Mediated Immune Responses in a Nonhuman Primate Model

    doi:

    Figure Lengend Snippet: Southern blot and PCR analysis for wt AAV DNA at the site of administration. (A) Southern blot of unamplified, Kpn I-digested genomic DNA isolated from the site of wt AAV administration. Liver tissue from animals infected intravenously (lanes 1 and 2), muscle tissue from animals infected intramuscularly (lanes 4 to 6), and nasal tissue from animals infected intranasally either without (lanes 8 and 9) or with (lanes 10 and 11) helper Ad were examined. Liver, muscle, and nasal epithelial DNAs from a saline-injected control animal were also examined (lanes 3, 7, and 12, respectively). Plasmid DNAs equating to 0.1, 1, 10, and 100 copies of wt AAV genomes per cell were included as standards (lanes 13 to 16, respectively). (B) PCR analysis for internal AAV-Rep gene sequences was performed for each of the same samples.

    Article Snippet: The DNA (30 μg) was digested for 24 h with Kpn I (New England BioLabs) under conditions recommended by the manufacturer.

    Techniques: Southern Blot, Polymerase Chain Reaction, Isolation, Infection, Injection, Plasmid Preparation

    In vitro selection process. ( A ) RNA aptamer library format, random region and tetraloop highlighted in black. ( B ) Fraction of RNA recovered from selections against BamHI (blue circles), KpnI (green triangles) and PacI (red squares), as a function of selection round.

    Journal: Nucleic Acids Research

    Article Title: RNA aptamer inhibitors of a restriction endonuclease

    doi: 10.1093/nar/gkv702

    Figure Lengend Snippet: In vitro selection process. ( A ) RNA aptamer library format, random region and tetraloop highlighted in black. ( B ) Fraction of RNA recovered from selections against BamHI (blue circles), KpnI (green triangles) and PacI (red squares), as a function of selection round.

    Article Snippet: Binding reactions contain radiolabeled aptamer (13 nM) and the indicated concentration of KpnI in New England Biolabs 1.1 buffer (10 mM Bis-Tris-Propane-HCl, pH 7, 10 mM MgCl2 , 100 μg/ml bovine serum albumin (BSA)) for 30 min at 37°C.

    Techniques: In Vitro, Selection

    Quantitation of KpnI binding affinity by electrophoretic gel mobility shift assay for ( A ) aptamer 20; ( B ) aptamer 24; ( C ) aptamer 29; and ( D ) aptamer 30. Replicate data are shown as black and gray points.

    Journal: Nucleic Acids Research

    Article Title: RNA aptamer inhibitors of a restriction endonuclease

    doi: 10.1093/nar/gkv702

    Figure Lengend Snippet: Quantitation of KpnI binding affinity by electrophoretic gel mobility shift assay for ( A ) aptamer 20; ( B ) aptamer 24; ( C ) aptamer 29; and ( D ) aptamer 30. Replicate data are shown as black and gray points.

    Article Snippet: Binding reactions contain radiolabeled aptamer (13 nM) and the indicated concentration of KpnI in New England Biolabs 1.1 buffer (10 mM Bis-Tris-Propane-HCl, pH 7, 10 mM MgCl2 , 100 μg/ml bovine serum albumin (BSA)) for 30 min at 37°C.

    Techniques: Quantitation Assay, Binding Assay, Mobility Shift

    Titration of 50 nM KpnI with high affinity radiolabeled aptamer 20 in the concentration range 100 pM–500 nM.

    Journal: Nucleic Acids Research

    Article Title: RNA aptamer inhibitors of a restriction endonuclease

    doi: 10.1093/nar/gkv702

    Figure Lengend Snippet: Titration of 50 nM KpnI with high affinity radiolabeled aptamer 20 in the concentration range 100 pM–500 nM.

    Article Snippet: Binding reactions contain radiolabeled aptamer (13 nM) and the indicated concentration of KpnI in New England Biolabs 1.1 buffer (10 mM Bis-Tris-Propane-HCl, pH 7, 10 mM MgCl2 , 100 μg/ml bovine serum albumin (BSA)) for 30 min at 37°C.

    Techniques: Titration, Concentration Assay

    Example of anti-KpnI aptamer 20 binding to KpnI as measured by quantitative electrophoretic gel mobility shift assay. A total of 13 nM radiolabeled RNA aptamer titrated with increasing concentrations of KpnI as indicated.

    Journal: Nucleic Acids Research

    Article Title: RNA aptamer inhibitors of a restriction endonuclease

    doi: 10.1093/nar/gkv702

    Figure Lengend Snippet: Example of anti-KpnI aptamer 20 binding to KpnI as measured by quantitative electrophoretic gel mobility shift assay. A total of 13 nM radiolabeled RNA aptamer titrated with increasing concentrations of KpnI as indicated.

    Article Snippet: Binding reactions contain radiolabeled aptamer (13 nM) and the indicated concentration of KpnI in New England Biolabs 1.1 buffer (10 mM Bis-Tris-Propane-HCl, pH 7, 10 mM MgCl2 , 100 μg/ml bovine serum albumin (BSA)) for 30 min at 37°C.

    Techniques: Binding Assay, Mobility Shift

    Example gels showing results of qualitative screens for aptamer activity. ( A ) Electrophoretic gel mobility shift binding screen. Radiolabeled aptamer candidates (4 nM) were exposed to their corresponding protein targets, in this case KpnI (20 nM; even lanes). ( B ) Restriction inhibition screen. Fluorescent DNA probe (20 nM, see panel C ) was exposed to REase that had been incubated with a high concentration of the indicated RNA aptamers (40 μM). The examples shown are RNA aptamers against KpnI.

    Journal: Nucleic Acids Research

    Article Title: RNA aptamer inhibitors of a restriction endonuclease

    doi: 10.1093/nar/gkv702

    Figure Lengend Snippet: Example gels showing results of qualitative screens for aptamer activity. ( A ) Electrophoretic gel mobility shift binding screen. Radiolabeled aptamer candidates (4 nM) were exposed to their corresponding protein targets, in this case KpnI (20 nM; even lanes). ( B ) Restriction inhibition screen. Fluorescent DNA probe (20 nM, see panel C ) was exposed to REase that had been incubated with a high concentration of the indicated RNA aptamers (40 μM). The examples shown are RNA aptamers against KpnI.

    Article Snippet: Binding reactions contain radiolabeled aptamer (13 nM) and the indicated concentration of KpnI in New England Biolabs 1.1 buffer (10 mM Bis-Tris-Propane-HCl, pH 7, 10 mM MgCl2 , 100 μg/ml bovine serum albumin (BSA)) for 30 min at 37°C.

    Techniques: Activity Assay, Mobility Shift, Binding Assay, Inhibition, Incubation, Concentration Assay

    Competition gel shi ft assay showing inhibition of KpnI binding to fluorescent DNA probe in the presence of 100 pM–10 μM anti-KpnI RNA aptamers. ( A ) Aptamer 20, ( B ) aptamer 24.

    Journal: Nucleic Acids Research

    Article Title: RNA aptamer inhibitors of a restriction endonuclease

    doi: 10.1093/nar/gkv702

    Figure Lengend Snippet: Competition gel shi ft assay showing inhibition of KpnI binding to fluorescent DNA probe in the presence of 100 pM–10 μM anti-KpnI RNA aptamers. ( A ) Aptamer 20, ( B ) aptamer 24.

    Article Snippet: Binding reactions contain radiolabeled aptamer (13 nM) and the indicated concentration of KpnI in New England Biolabs 1.1 buffer (10 mM Bis-Tris-Propane-HCl, pH 7, 10 mM MgCl2 , 100 μg/ml bovine serum albumin (BSA)) for 30 min at 37°C.

    Techniques: Inhibition, Binding Assay

    ( A ) In-line probing ( 36 ) of high-affinity anti-KpnI aptamers 20, 24, 29, 30. (UN: unmodified samples (lane 1, 6, 11 and 16); T1: RNAse T1 digestions (lane 2, 7, 12 and 17); OH: alkaline hydrolysis reactions (lane 3, 8, 13 and 18); IN: In-line attack reactions after 24 h (lane 4, 9, 14 and 19) and 48 h (lane 5, 10, 15 and 20). ( B ) Predicted secondary structure of aptamer 20 and validation by in-line probing shown as color-coded heat map (red, high in-line attack rate; white, intermediate in-line attack rate; blue, low in-line attack rate). Black box highlights the nucleotides that were randomized in the original library.

    Journal: Nucleic Acids Research

    Article Title: RNA aptamer inhibitors of a restriction endonuclease

    doi: 10.1093/nar/gkv702

    Figure Lengend Snippet: ( A ) In-line probing ( 36 ) of high-affinity anti-KpnI aptamers 20, 24, 29, 30. (UN: unmodified samples (lane 1, 6, 11 and 16); T1: RNAse T1 digestions (lane 2, 7, 12 and 17); OH: alkaline hydrolysis reactions (lane 3, 8, 13 and 18); IN: In-line attack reactions after 24 h (lane 4, 9, 14 and 19) and 48 h (lane 5, 10, 15 and 20). ( B ) Predicted secondary structure of aptamer 20 and validation by in-line probing shown as color-coded heat map (red, high in-line attack rate; white, intermediate in-line attack rate; blue, low in-line attack rate). Black box highlights the nucleotides that were randomized in the original library.

    Article Snippet: Binding reactions contain radiolabeled aptamer (13 nM) and the indicated concentration of KpnI in New England Biolabs 1.1 buffer (10 mM Bis-Tris-Propane-HCl, pH 7, 10 mM MgCl2 , 100 μg/ml bovine serum albumin (BSA)) for 30 min at 37°C.

    Techniques:

    KpnI inhibition by anti-KpnI RNA aptamers. KpnI in the presence of 20 nM fluorescent DNA probe was incubated with anti-KpnI aptamers in the concentration range 10 pM–100 μM. ( A ) Aptamer 20; ( B ) aptamer 24; ( C ) aptamer 29; ( D ) aptamer 30.

    Journal: Nucleic Acids Research

    Article Title: RNA aptamer inhibitors of a restriction endonuclease

    doi: 10.1093/nar/gkv702

    Figure Lengend Snippet: KpnI inhibition by anti-KpnI RNA aptamers. KpnI in the presence of 20 nM fluorescent DNA probe was incubated with anti-KpnI aptamers in the concentration range 10 pM–100 μM. ( A ) Aptamer 20; ( B ) aptamer 24; ( C ) aptamer 29; ( D ) aptamer 30.

    Article Snippet: Binding reactions contain radiolabeled aptamer (13 nM) and the indicated concentration of KpnI in New England Biolabs 1.1 buffer (10 mM Bis-Tris-Propane-HCl, pH 7, 10 mM MgCl2 , 100 μg/ml bovine serum albumin (BSA)) for 30 min at 37°C.

    Techniques: Inhibition, Incubation, Concentration Assay

    Restriction digestion patterns of PERV clones. A , Five patterns (A-E) of MboI digestion and B , four patterns (a-d) of KpnI digestion were identified in the PCR product amplified by Easy A polymerase high fidelity.

    Journal: Virology Journal

    Article Title: Characterization of PERV in a new conserved pig herd as potential donor animals for xenotransplantation in China

    doi: 10.1186/s12985-014-0212-1

    Figure Lengend Snippet: Restriction digestion patterns of PERV clones. A , Five patterns (A-E) of MboI digestion and B , four patterns (a-d) of KpnI digestion were identified in the PCR product amplified by Easy A polymerase high fidelity.

    Article Snippet: Three hundred single positive clones were picked for PCR amplification, and the PCR products were analyzed using the restriction enzymes KpnI and MboI (New England Biolabs, MA, USA).

    Techniques: Clone Assay, Polymerase Chain Reaction, Amplification

    Genetic and restriction map of the constructed plasmids. A ( a ) pCMV6-XL5-SMN digested with NotI, ( b ) pAAV-CB6-PI was digested with EcoRI and KpnI, to construct ( c ) pAAVsc-CB6-PI- SMN. B ( d ) pCMV6-XL5-SMN was digested with Bgl II, ( e ) pAAVsc-CB6-PI was

    Journal: Molecular biotechnology

    Article Title: Large-Scale Production of Adeno-Associated Viral Vector Serotype-9 Carrying the Human Survival Motor Neuron Gene

    doi: 10.1007/s12033-015-9899-5

    Figure Lengend Snippet: Genetic and restriction map of the constructed plasmids. A ( a ) pCMV6-XL5-SMN digested with NotI, ( b ) pAAV-CB6-PI was digested with EcoRI and KpnI, to construct ( c ) pAAVsc-CB6-PI- SMN. B ( d ) pCMV6-XL5-SMN was digested with Bgl II, ( e ) pAAVsc-CB6-PI was

    Article Snippet: The pAAV-CB6-PI and pAAVsc-CB6-PI plasmids were digested with EcoRI/KpnI and AgeI/SacI (New England Biolabs, MA, USA) restriction enzymes.

    Techniques: Construct