Structured Review

Toyobo kod fx neo
RFLP using direct <t>PCR</t> products with <t>KOD</t> FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .
Kod Fx Neo, supplied by Toyobo, used in various techniques. Bioz Stars score: 94/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kod fx neo/product/Toyobo
Average 94 stars, based on 327 article reviews
Price from $9.99 to $1999.99
kod fx neo - by Bioz Stars, 2020-09
94/100 stars

Images

1) Product Images from "Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR"

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

Journal: Kobe Journal of Medical Sciences

doi:

RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .
Figure Legend Snippet: RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .

Techniques Used: Polymerase Chain Reaction

2) Product Images from "Zygosity Determination in Hairless Mice by PCR Based onHrhr Gene Analysis"

Article Title: Zygosity Determination in Hairless Mice by PCR Based onHrhr Gene Analysis

Journal: Experimental Animals

doi: 10.1538/expanim.62.267

Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and HotStarTaq was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.
Figure Legend Snippet: Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and HotStarTaq was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.

Techniques Used: Mouse Assay, Sequencing, Mutagenesis, Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis

3) Product Images from "Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR"

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

Journal: Kobe Journal of Medical Sciences

doi:

RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .
Figure Legend Snippet: RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .

Techniques Used: Polymerase Chain Reaction

4) Product Images from "Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR"

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

Journal: Kobe Journal of Medical Sciences

doi:

Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .
Figure Legend Snippet: Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .

Techniques Used: DNA Extraction

RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .
Figure Legend Snippet: RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .

Techniques Used: Polymerase Chain Reaction

5) Product Images from "Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR"

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

Journal: Kobe Journal of Medical Sciences

doi:

Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .
Figure Legend Snippet: Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .

Techniques Used: DNA Extraction

RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .
Figure Legend Snippet: RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .

Techniques Used: Polymerase Chain Reaction

6) Product Images from "Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR"

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

Journal: Kobe Journal of Medical Sciences

doi:

Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .
Figure Legend Snippet: Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .

Techniques Used: DNA Extraction

7) Product Images from "Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR"

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

Journal: Kobe Journal of Medical Sciences

doi:

RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .
Figure Legend Snippet: RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .

Techniques Used: Polymerase Chain Reaction

8) Product Images from "Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR"

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

Journal: Kobe Journal of Medical Sciences

doi:

Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .
Figure Legend Snippet: Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .

Techniques Used: DNA Extraction

Related Articles

Polymerase Chain Reaction:

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. It should be noted that PCR with DNA preparation method (E) using KOD FX Neo™ also generated high amount of PCR products at 35 and 40 cycles. .. The difference of PCR amplification efficiency with KOD FX Neo™ between (E) and others (A–D) may be due to the abundance of DNA.

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. In conclusion, direct PCR with DNA polymerases like KOD FX Neo™ can be widely used in newborn screening for SMA in the near future, because it can skip the process of DNA extraction from DBS and precisely amplify the target sequences in spite of the presence of PCR inhibitors. .. We previously established a DBS-based SMA screening with a modified competitive PCR (mCOP-PCR) technique .

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. The difference of PCR amplification efficiency with KOD FX Neo™ between (E) and others (A–D) may be due to the abundance of DNA. .. DNA in samples (E) was much more abundant than DNA in samples (A–D); one half of the DBS disk (E), which was used as template for PCR, was much more abundant in DNA than 2 μL of DNA solution of (A), (B), (C) and (D).

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. Whereas, when KOD FX Neo™ was used, PCR amplification was successful without DNA extraction process; PCR products at both 35 and 40 cycles were clearly detected on the gel electrophoresis ( ). .. These results indicate that KOD FX Neo™ is much less affected by PCR inhibitors presented in the DBS paper, and that we are able to perform direct PCR from the DBS paper without DNA extraction process.

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. These results indicate that KOD FX Neo™ is much less affected by PCR inhibitors presented in the DBS paper, and that we are able to perform direct PCR from the DBS paper without DNA extraction process. .. We have known from our experience that KOD FX Neo™ polymerase is useful for long-range PCR targeting at amplification of more than 25Kb sized DNA such as SMN ), “the KOD FX Neo™ is able to amplify DNA from crude samples and can reduce influence of PCR inhibitors within soil, food, etc.” Our results clearly proved that KOD FX Neo™ polymerase is useful for the PCR amplification from DBS which can be considered as a kind of crude sample.

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. We have known from our experience that KOD FX Neo™ polymerase is useful for long-range PCR targeting at amplification of more than 25Kb sized DNA such as SMN ), “the KOD FX Neo™ is able to amplify DNA from crude samples and can reduce influence of PCR inhibitors within soil, food, etc.” Our results clearly proved that KOD FX Neo™ polymerase is useful for the PCR amplification from DBS which can be considered as a kind of crude sample. .. Although the company emphasizes that KOD FX Neo™ overcomes PCR inhibitors in the crude samples, it may be empirical data, because we failed to get clear explanation about the mechanism of KOD FX Neo™ in the previous literatures.

DNA Extraction:

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. In conclusion, direct PCR with DNA polymerases like KOD FX Neo™ can be widely used in newborn screening for SMA in the near future, because it can skip the process of DNA extraction from DBS and precisely amplify the target sequences in spite of the presence of PCR inhibitors. .. We previously established a DBS-based SMA screening with a modified competitive PCR (mCOP-PCR) technique .

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. Whereas, when KOD FX Neo™ was used, PCR amplification was successful without DNA extraction process; PCR products at both 35 and 40 cycles were clearly detected on the gel electrophoresis ( ). .. These results indicate that KOD FX Neo™ is much less affected by PCR inhibitors presented in the DBS paper, and that we are able to perform direct PCR from the DBS paper without DNA extraction process.

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. These results indicate that KOD FX Neo™ is much less affected by PCR inhibitors presented in the DBS paper, and that we are able to perform direct PCR from the DBS paper without DNA extraction process. .. We have known from our experience that KOD FX Neo™ polymerase is useful for long-range PCR targeting at amplification of more than 25Kb sized DNA such as SMN ), “the KOD FX Neo™ is able to amplify DNA from crude samples and can reduce influence of PCR inhibitors within soil, food, etc.” Our results clearly proved that KOD FX Neo™ polymerase is useful for the PCR amplification from DBS which can be considered as a kind of crude sample.

Generated:

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. It should be noted that PCR with DNA preparation method (E) using KOD FX Neo™ also generated high amount of PCR products at 35 and 40 cycles. .. The difference of PCR amplification efficiency with KOD FX Neo™ between (E) and others (A–D) may be due to the abundance of DNA.

Amplification:

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. The difference of PCR amplification efficiency with KOD FX Neo™ between (E) and others (A–D) may be due to the abundance of DNA. .. DNA in samples (E) was much more abundant than DNA in samples (A–D); one half of the DBS disk (E), which was used as template for PCR, was much more abundant in DNA than 2 μL of DNA solution of (A), (B), (C) and (D).

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. Whereas, when KOD FX Neo™ was used, PCR amplification was successful without DNA extraction process; PCR products at both 35 and 40 cycles were clearly detected on the gel electrophoresis ( ). .. These results indicate that KOD FX Neo™ is much less affected by PCR inhibitors presented in the DBS paper, and that we are able to perform direct PCR from the DBS paper without DNA extraction process.

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. We have known from our experience that KOD FX Neo™ polymerase is useful for long-range PCR targeting at amplification of more than 25Kb sized DNA such as SMN ), “the KOD FX Neo™ is able to amplify DNA from crude samples and can reduce influence of PCR inhibitors within soil, food, etc.” Our results clearly proved that KOD FX Neo™ polymerase is useful for the PCR amplification from DBS which can be considered as a kind of crude sample. .. Although the company emphasizes that KOD FX Neo™ overcomes PCR inhibitors in the crude samples, it may be empirical data, because we failed to get clear explanation about the mechanism of KOD FX Neo™ in the previous literatures.

Nucleic Acid Electrophoresis:

Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR
Article Snippet: .. Whereas, when KOD FX Neo™ was used, PCR amplification was successful without DNA extraction process; PCR products at both 35 and 40 cycles were clearly detected on the gel electrophoresis ( ). .. These results indicate that KOD FX Neo™ is much less affected by PCR inhibitors presented in the DBS paper, and that we are able to perform direct PCR from the DBS paper without DNA extraction process.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Toyobo kod fx neo
    RFLP using direct <t>PCR</t> products with <t>KOD</t> FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .
    Kod Fx Neo, supplied by Toyobo, used in various techniques. Bioz Stars score: 94/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kod fx neo/product/Toyobo
    Average 94 stars, based on 327 article reviews
    Price from $9.99 to $1999.99
    kod fx neo - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .

    Journal: Kobe Journal of Medical Sciences

    Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

    doi:

    Figure Lengend Snippet: RFLP using direct PCR products with KOD FX Neo ™ polymerase (1) Before DraI digestion. The bands represent the mixture of PCR products from SMN1 and/or SMN2 . (2) After DraI digestion. Upper bands represent PCR products from SMN1 and lower bands PCR products from SMN2 . DraI digestion proved the presence of SMN1 and SMN2 in the PCR products of the healthy volunteers and the absence of SMN1 and the presence of SMN2 in the SMA patients. All SMA patients showed the absence of SMN1 .

    Article Snippet: The difference of PCR amplification efficiency with KOD FX Neo™ between (E) and others (A–D) may be due to the abundance of DNA.

    Techniques: Polymerase Chain Reaction

    Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and HotStarTaq was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.

    Journal: Experimental Animals

    Article Title: Zygosity Determination in Hairless Mice by PCR Based onHrhr Gene Analysis

    doi: 10.1538/expanim.62.267

    Figure Lengend Snippet: Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and HotStarTaq was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.

    Article Snippet: The difference between homozygous (Hrx /Hrx ) and wild-type (Hr /Hr ) genomes was determined using multiple PCRs with HotStarTaq (#203443, Qiagen; regions 1, 8–13) or KOD FX neo (KFX-201, TOYOBO, Osaka, Japan; regions 2–7) DNA polymerases.

    Techniques: Mouse Assay, Sequencing, Mutagenesis, Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis

    Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .

    Journal: Kobe Journal of Medical Sciences

    Article Title: Newborn Screening for Spinal Muscular Atrophy: DNA Preparation from Dried Blood Spot and DNA Polymerase Selection in PCR

    doi:

    Figure Lengend Snippet: Comparison between DNA preparations and between DNA polymerases DNA preparation methods were as follows: (A) DNA extraction by a simple “boiling” method, (B) DNA extraction by a “rinsing and boiling” method, (C) DNA extraction by a “protein fixation-1, rinsing and boiling” method, (D) DNA extraction by a “protein fixation-2, rinsing and boiling” method, and (E) No DNA extraction process. (A), (B), (C) and (D) were grouped into the “DNA extraction” method, while (E) was grouped into the “non-DNA-extraction” method. DNA polymerases tested in this study were TaKaRa Ex Taq ™ and KOD FX Neo ™ .

    Article Snippet: In conclusion, direct PCR with DNA polymerases like KOD FX Neo™ can be widely used in newborn screening for SMA in the near future, because it can skip the process of DNA extraction from DBS and precisely amplify the target sequences in spite of the presence of PCR inhibitors.

    Techniques: DNA Extraction