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Toyobo kod fx neo dna polymerase
Kod Fx Neo Dna Polymerase, supplied by Toyobo, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kod fx neo dna polymerase/product/Toyobo
Average 91 stars, based on 7 article reviews
Price from $9.99 to $1999.99
kod fx neo dna polymerase - by Bioz Stars, 2020-08
91/100 stars

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Amplification:

Article Title: Efficient PCR-Based Amplification of Diverse Alcohol Dehydrogenase Genes from Metagenomes for Improving Biocatalysis: Screening of Gene-Specific Amplicons from Metagenomes
Article Snippet: .. We used hot-start and step-down PCR protocols to avoid nonspecific amplification and to support the sufficient amplification of DNA using KOD FX Neo DNA polymerase or Phusion hot-start flex DNA polymerase. ..

Article Title: AtUBL5 regulates growth and development through pre-mRNA splicing in Arabidopsis thaliana
Article Snippet: .. The AtPRP38 cDNA (1,065 bp) was amplified with KOD FX Neo DNA polymerase (Toyobo, Osaka, Japan) using the AtPRP38 primer set , and then introduced to the Gateway entry vector pENTR™ /D-TOPO (Thermo Fisher Scientific) to yield pENTR-AtPRP38. .. The C-terminal region of AtPRP38 cDNA as amplified with KOD FX Neo DNA polymerase (Toyobo) using the AtPRP38C primer set , and then introduced to the Gateway entry vector pENTR™ /D-TOPO (Thermo Fisher Scientific) to yield pENTR-AtPRP38C.

Inverse PCR:

Article Title: Identification of a novel zinc-binding protein, C1orf123, as an interactor with a heavy metal-associated domain
Article Snippet: .. Mutations were introduced by inverse PCR using KOD-FX-Neo DNA polymerase (TOYOBO). ..

Isolation:

Article Title: Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein–coupled receptors
Article Snippet: .. Genomic DNA was extracted from the isolated cells and subjected to PCR using KOD FX Neo DNA polymerase (Toyobo). .. Three sets of primers (Supplemental Table S3) were used to distinguish the following three states of integration of the donor knock-in vector: forward integration, reverse integration, and no integration with a small insertion or deletion (Supplemental Figure S1, A and D).

Polymerase Chain Reaction:

Article Title: The Endosymbiotic Bacterium Wolbachia Selectively Kills Male Hosts by Targeting the Masculinizing Gene
Article Snippet: .. PCR was carried out with KOD FX-neo DNA polymerase (TOYOBO). .. Sex-specific splicing of Ostrinia dsx by RT-PCR and Wolbachia detection by wsp PCR were performed with primers reported previously [ ].

Article Title: Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein–coupled receptors
Article Snippet: .. Genomic DNA was extracted from the isolated cells and subjected to PCR using KOD FX Neo DNA polymerase (Toyobo). .. Three sets of primers (Supplemental Table S3) were used to distinguish the following three states of integration of the donor knock-in vector: forward integration, reverse integration, and no integration with a small insertion or deletion (Supplemental Figure S1, A and D).

Article Title: Refrex-1, a Soluble Restriction Factor against Feline Endogenous and Exogenous Retroviruses
Article Snippet: .. KOD FX Neo DNA polymerase (Toyobo, Osaka, Japan) was used for PCR. .. The PCR products were cloned into pCR-Blunt (Invitrogen, Carlsbad, CA) and sequenced.

Article Title: Efficient PCR-Based Amplification of Diverse Alcohol Dehydrogenase Genes from Metagenomes for Improving Biocatalysis: Screening of Gene-Specific Amplicons from Metagenomes
Article Snippet: .. We used hot-start and step-down PCR protocols to avoid nonspecific amplification and to support the sufficient amplification of DNA using KOD FX Neo DNA polymerase or Phusion hot-start flex DNA polymerase. ..

Article Title: The Composition and Structure of Biofilms Developed by Propionibacterium acnes Isolated from Cardiac Pacemaker Devices
Article Snippet: .. PCR was performed in a total volume of 50 μl containing 1 ml of crude DNA extract, 1× PCR buffer, 0.4 mM dNTPs, 0.3 μM forward and reverse primers, and 1.0 U KOD FX Neo DNA polymerase (Toyobo Co., Ltd., Osaka, Japan). .. PCR reaction conditions were as follows: initial denaturation for 2 min at 94°C and 30 cycles consisting of 10 s at 98°C, 30 s at 53°C, and 1.5 min at 68°C.

Expressing:

Article Title: Imaging endogenous synaptic proteins in primary neurons at single-cell resolution using CRISPR/Cas9
Article Snippet: .. To detect the expression of PSD-95-EGFP fusion gene, RT-PCR was carried out using KOD FX Neo DNA polymerase (Toyobo, Osaka, Japan), and primer sets (5´-TGCACTATGCTCGTCCCATCATCATC-3´ and 5´-GACACGCTGAACTTGTGGCCGTTTACG -3´, product size 754 base pairs) under the following conditions: one cycle of 98 °C for 3 min, 38 cycles of 98 °C for 10 s, and 68 °C for 1 min. .. The expression of mCherry from pCAG-mCherry was detected by RT-PCR with primers (5´-TAACATGGCCATCATCAAGGAGTTC-3´ and 5´-AGCCCATGGTCTT­CTTCTGCATTAC-3´, product size 309 base pairs) under the following conditions: one cycle of 98°C for 3 min, 35 cycles of 98°C for 10 s, 62°C for 15 s, and 68°C for 30 s. The expression of Actb was detected by RT-PCR with primers (5′-ATGTACGTAGCCATCCAGGCTGT-3′ and 5′-AGCTGTGGTGGTGAAGCTGTAG-3′, product size 419 base pairs) under the following conditions: one cycle of 98°C for 3 min, 25 cycles of 98°C for 10 s, 60°C for 10 s, and 68°C for 30 s. Quantitative RT-PCR was performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using KOD SYBR qPCR Mix (Toyobo, Osaka, Japan) and the primers described above.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Imaging endogenous synaptic proteins in primary neurons at single-cell resolution using CRISPR/Cas9
Article Snippet: .. To detect the expression of PSD-95-EGFP fusion gene, RT-PCR was carried out using KOD FX Neo DNA polymerase (Toyobo, Osaka, Japan), and primer sets (5´-TGCACTATGCTCGTCCCATCATCATC-3´ and 5´-GACACGCTGAACTTGTGGCCGTTTACG -3´, product size 754 base pairs) under the following conditions: one cycle of 98 °C for 3 min, 38 cycles of 98 °C for 10 s, and 68 °C for 1 min. .. The expression of mCherry from pCAG-mCherry was detected by RT-PCR with primers (5´-TAACATGGCCATCATCAAGGAGTTC-3´ and 5´-AGCCCATGGTCTT­CTTCTGCATTAC-3´, product size 309 base pairs) under the following conditions: one cycle of 98°C for 3 min, 35 cycles of 98°C for 10 s, 62°C for 15 s, and 68°C for 30 s. The expression of Actb was detected by RT-PCR with primers (5′-ATGTACGTAGCCATCCAGGCTGT-3′ and 5′-AGCTGTGGTGGTGAAGCTGTAG-3′, product size 419 base pairs) under the following conditions: one cycle of 98°C for 3 min, 25 cycles of 98°C for 10 s, 60°C for 10 s, and 68°C for 30 s. Quantitative RT-PCR was performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) using KOD SYBR qPCR Mix (Toyobo, Osaka, Japan) and the primers described above.

Plasmid Preparation:

Article Title: AtUBL5 regulates growth and development through pre-mRNA splicing in Arabidopsis thaliana
Article Snippet: .. The AtPRP38 cDNA (1,065 bp) was amplified with KOD FX Neo DNA polymerase (Toyobo, Osaka, Japan) using the AtPRP38 primer set , and then introduced to the Gateway entry vector pENTR™ /D-TOPO (Thermo Fisher Scientific) to yield pENTR-AtPRP38. .. The C-terminal region of AtPRP38 cDNA as amplified with KOD FX Neo DNA polymerase (Toyobo) using the AtPRP38C primer set , and then introduced to the Gateway entry vector pENTR™ /D-TOPO (Thermo Fisher Scientific) to yield pENTR-AtPRP38C.

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  • 91
    Toyobo fidelity dna polymerases
    <t>PCR</t> product patterns amplified by the primer pair CS1-F/CS1-R targeting the partial S gene. Samples from left to right: (1) JAi-23, (2) JMi-69, (3) JMi-124, (4) JKa-230, (5) JA0-56, (6) JMi-231, (M) <t>DNA</t> marker. The dashed arrows indicate products of PEDV variants with large genomic deletions (approximately 0.2–0.3 kbp) and the solid arrow indicates the predicted product (872 bp).
    Fidelity Dna Polymerases, supplied by Toyobo, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fidelity dna polymerases/product/Toyobo
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fidelity dna polymerases - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    89
    Toyobo dna polymerase kod fx neo
    <t>PCR</t> product patterns amplified by the primer pair CS1-F/CS1-R targeting the partial S gene. Samples from left to right: (1) JAi-23, (2) JMi-69, (3) JMi-124, (4) JKa-230, (5) JA0-56, (6) JMi-231, (M) <t>DNA</t> marker. The dashed arrows indicate products of PEDV variants with large genomic deletions (approximately 0.2–0.3 kbp) and the solid arrow indicates the predicted product (872 bp).
    Dna Polymerase Kod Fx Neo, supplied by Toyobo, used in various techniques. Bioz Stars score: 89/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase kod fx neo/product/Toyobo
    Average 89 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    dna polymerase kod fx neo - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    Image Search Results


    PCR product patterns amplified by the primer pair CS1-F/CS1-R targeting the partial S gene. Samples from left to right: (1) JAi-23, (2) JMi-69, (3) JMi-124, (4) JKa-230, (5) JA0-56, (6) JMi-231, (M) DNA marker. The dashed arrows indicate products of PEDV variants with large genomic deletions (approximately 0.2–0.3 kbp) and the solid arrow indicates the predicted product (872 bp).

    Journal: PLoS ONE

    Article Title: Novel Porcine Epidemic Diarrhea Virus (PEDV) Variants with Large Deletions in the Spike (S) Gene Coexist with PEDV Strains Possessing an Intact S Gene in Domestic Pigs in Japan: A New Disease Situation

    doi: 10.1371/journal.pone.0170126

    Figure Lengend Snippet: PCR product patterns amplified by the primer pair CS1-F/CS1-R targeting the partial S gene. Samples from left to right: (1) JAi-23, (2) JMi-69, (3) JMi-124, (4) JKa-230, (5) JA0-56, (6) JMi-231, (M) DNA marker. The dashed arrows indicate products of PEDV variants with large genomic deletions (approximately 0.2–0.3 kbp) and the solid arrow indicates the predicted product (872 bp).

    Article Snippet: The same result was observed when one-step RT-PCR tests (AccessQuick RT-PCR System kit, Promega Corp, WI, USA) were performed from extracted RNA and PCR tests using other kits containing high fidelity DNA polymerases (KOD FX, KOD FX neo, and Blend–Tag Plus Kits, Toyobo Co., Japan) were performed from cDNA templates to amplify the CS1 fragment.

    Techniques: Polymerase Chain Reaction, Amplification, Marker