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goat anti klf4  (R&D Systems)


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    Structured Review

    R&D Systems goat anti klf4
    Goat Anti Klf4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/klf4/Mouse+KLF4+Antibody/us12582698-2007-34-37
    Average 95 stars, based on 181 article reviews
    goat anti klf4 - by Bioz Stars, 2026-09
    95/100 stars

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    other:

    Article Title: Spatial multi-omics reveals cell-type-specific nuclear compartments.
    Article Snippet: The mammalian nucleus is compartmentalized by diverse subnuclear structures.. These subnuclear structures, marked by nuclear bodies and histone modifications, are often cell-type specific and affect gene regulation and 3D genome organization.. Understanding their relationships rests on identifying the molecular constituents of subnuclear structures and mapping their associations with specific genomic loci and transcriptional levels in individual cells, all in complex tissues.

    Chromatin Immunoprecipitation:

    Article Title: Nrf2 Drives Epigenetic Reprogramming and Acts as the Master Regulator of KLF4 Expression and Activity in Arsenic-Induced Transformation.
    Article Snippet: DNA concentration was measured using a NanoDrop spectrophotometer (Thermo Scientific). .. A 30 μg aliquot of chromatin was precleared with protein A agarose beads (Invitrogen), and ChIP was performed using 4 μg of antibodies against H3K4me1 (Thermofisher, cat#710795), H3K4me3 (Thermofisher, cat#711958), H3K9me3 (CST, cat#4658), H3K27Ac (CST, cat#8173), H3K27me3 (CST, cat#9733), H3K36me3 (CST, cat#4909), H4K20me3 (CST, cat#5737) and KLF4 (R&D Systems, catAF3640). ..

    Article Title: Poised PABP-RNA hubs implement signal-dependent mRNA decay in development.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies used for cell immunoflourescence LIN28A (A177 Cell Signaling and ab63740 Abcam) Klf4 (AF3158, R&D Systems) Nanog (8822, Cell Signaling) Antibodies used for FACS experiments SSEA1 (MC480, Thermo) SSEA4 (MC813-70, Thermo) Antibodies used for Western blotting LIN28A (A177 Cell Signaling and AF3757, R&D Systems) H3 (Abcam, ab1791) 4 nature portfolio | reporting sum m ary April 2023 GAPDH (Cell Signaling, 2118S) Antibodies used for iCLIP GFP polyclonal Antibody - Thermo Fisher A6455 LIN28A polyclonal Antibody - Cell signaling #3978 PABPC1 - Abcam ab21060 and Proteintech 10970-1-AP PABPC4 - Proteintech 14960-1-AP Validation Antibodies used for Western blotting and cell immunoflourescence: LIN28A (A177 Cell Signaling). .. An antibody routinely used as a first choice for LIN28A immunoprecipitations, Western Blots and immunoflourescence: Product website outlines 42 relevant citations (https://www.cellsignal.co.uk/products/primary-antibodies/ lin28a-a177-antibody/3978?N=0+4294956287&Nrpp=200&No=3200&fromPage=plp), including publications that validated the specificity of A177 LIN28A Ab when compared to a cell line not expressing LIN28A (PMID: 27992407).

    Article Title: Nrf2 Drives Epigenetic Reprogramming and Acts as the Master Regulator of KLF4 Expression and Activity in Arsenic‐Induced Transformation
    Article Snippet: DNA concentration was measured using a NanoDrop spectrophotometer (Thermo Scientific). .. A 30 μg aliquot of chromatin was precleared with protein A agarose beads (Invitrogen), and ChIP was performed using 4 μg of antibodies against H3K4me1 (Thermofisher, cat#710795), H3K4me3 (Thermofisher, cat#711958), H3K9me3 (CST, cat#4658), H3K27Ac (CST, cat#8173), H3K27me3 (CST, cat#9733), H3K36me3 (CST, cat#4909), H4K20me3 (CST, cat#5737) and KLF4 (R&D Systems, catAF3640). ..

    Flow Cytometry:

    Article Title: Poised PABP-RNA hubs implement signal-dependent mRNA decay in development.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies used for cell immunoflourescence LIN28A (A177 Cell Signaling and ab63740 Abcam) Klf4 (AF3158, R&D Systems) Nanog (8822, Cell Signaling) Antibodies used for FACS experiments SSEA1 (MC480, Thermo) SSEA4 (MC813-70, Thermo) Antibodies used for Western blotting LIN28A (A177 Cell Signaling and AF3757, R&D Systems) H3 (Abcam, ab1791) 4 nature portfolio | reporting sum m ary April 2023 GAPDH (Cell Signaling, 2118S) Antibodies used for iCLIP GFP polyclonal Antibody - Thermo Fisher A6455 LIN28A polyclonal Antibody - Cell signaling #3978 PABPC1 - Abcam ab21060 and Proteintech 10970-1-AP PABPC4 - Proteintech 14960-1-AP Validation Antibodies used for Western blotting and cell immunoflourescence: LIN28A (A177 Cell Signaling). .. An antibody routinely used as a first choice for LIN28A immunoprecipitations, Western Blots and immunoflourescence: Product website outlines 42 relevant citations (https://www.cellsignal.co.uk/products/primary-antibodies/ lin28a-a177-antibody/3978?N=0+4294956287&Nrpp=200&No=3200&fromPage=plp), including publications that validated the specificity of A177 LIN28A Ab when compared to a cell line not expressing LIN28A (PMID: 27992407).

    Magnetic Resonance Imaging:

    Article Title: Poised PABP-RNA hubs implement signal-dependent mRNA decay in development.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies used for cell immunoflourescence LIN28A (A177 Cell Signaling and ab63740 Abcam) Klf4 (AF3158, R&D Systems) Nanog (8822, Cell Signaling) Antibodies used for FACS experiments SSEA1 (MC480, Thermo) SSEA4 (MC813-70, Thermo) Antibodies used for Western blotting LIN28A (A177 Cell Signaling and AF3757, R&D Systems) H3 (Abcam, ab1791) 4 nature portfolio | reporting sum m ary April 2023 GAPDH (Cell Signaling, 2118S) Antibodies used for iCLIP GFP polyclonal Antibody - Thermo Fisher A6455 LIN28A polyclonal Antibody - Cell signaling #3978 PABPC1 - Abcam ab21060 and Proteintech 10970-1-AP PABPC4 - Proteintech 14960-1-AP Validation Antibodies used for Western blotting and cell immunoflourescence: LIN28A (A177 Cell Signaling). .. An antibody routinely used as a first choice for LIN28A immunoprecipitations, Western Blots and immunoflourescence: Product website outlines 42 relevant citations (https://www.cellsignal.co.uk/products/primary-antibodies/ lin28a-a177-antibody/3978?N=0+4294956287&Nrpp=200&No=3200&fromPage=plp), including publications that validated the specificity of A177 LIN28A Ab when compared to a cell line not expressing LIN28A (PMID: 27992407).

    FACS:

    Article Title: Poised PABP-RNA hubs implement signal-dependent mRNA decay in development.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies used for cell immunoflourescence LIN28A (A177 Cell Signaling and ab63740 Abcam) Klf4 (AF3158, R&D Systems) Nanog (8822, Cell Signaling) Antibodies used for FACS experiments SSEA1 (MC480, Thermo) SSEA4 (MC813-70, Thermo) Antibodies used for Western blotting LIN28A (A177 Cell Signaling and AF3757, R&D Systems) H3 (Abcam, ab1791) 4 nature portfolio | reporting sum m ary April 2023 GAPDH (Cell Signaling, 2118S) Antibodies used for iCLIP GFP polyclonal Antibody - Thermo Fisher A6455 LIN28A polyclonal Antibody - Cell signaling #3978 PABPC1 - Abcam ab21060 and Proteintech 10970-1-AP PABPC4 - Proteintech 14960-1-AP Validation Antibodies used for Western blotting and cell immunoflourescence: LIN28A (A177 Cell Signaling). .. An antibody routinely used as a first choice for LIN28A immunoprecipitations, Western Blots and immunoflourescence: Product website outlines 42 relevant citations (https://www.cellsignal.co.uk/products/primary-antibodies/ lin28a-a177-antibody/3978?N=0+4294956287&Nrpp=200&No=3200&fromPage=plp), including publications that validated the specificity of A177 LIN28A Ab when compared to a cell line not expressing LIN28A (PMID: 27992407).

    Western Blot:

    Article Title: Poised PABP-RNA hubs implement signal-dependent mRNA decay in development.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies used for cell immunoflourescence LIN28A (A177 Cell Signaling and ab63740 Abcam) Klf4 (AF3158, R&D Systems) Nanog (8822, Cell Signaling) Antibodies used for FACS experiments SSEA1 (MC480, Thermo) SSEA4 (MC813-70, Thermo) Antibodies used for Western blotting LIN28A (A177 Cell Signaling and AF3757, R&D Systems) H3 (Abcam, ab1791) 4 nature portfolio | reporting sum m ary April 2023 GAPDH (Cell Signaling, 2118S) Antibodies used for iCLIP GFP polyclonal Antibody - Thermo Fisher A6455 LIN28A polyclonal Antibody - Cell signaling #3978 PABPC1 - Abcam ab21060 and Proteintech 10970-1-AP PABPC4 - Proteintech 14960-1-AP Validation Antibodies used for Western blotting and cell immunoflourescence: LIN28A (A177 Cell Signaling). .. An antibody routinely used as a first choice for LIN28A immunoprecipitations, Western Blots and immunoflourescence: Product website outlines 42 relevant citations (https://www.cellsignal.co.uk/products/primary-antibodies/ lin28a-a177-antibody/3978?N=0+4294956287&Nrpp=200&No=3200&fromPage=plp), including publications that validated the specificity of A177 LIN28A Ab when compared to a cell line not expressing LIN28A (PMID: 27992407).

    Biomarker Discovery:

    Article Title: Poised PABP-RNA hubs implement signal-dependent mRNA decay in development.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Antibodies used for cell immunoflourescence LIN28A (A177 Cell Signaling and ab63740 Abcam) Klf4 (AF3158, R&D Systems) Nanog (8822, Cell Signaling) Antibodies used for FACS experiments SSEA1 (MC480, Thermo) SSEA4 (MC813-70, Thermo) Antibodies used for Western blotting LIN28A (A177 Cell Signaling and AF3757, R&D Systems) H3 (Abcam, ab1791) 4 nature portfolio | reporting sum m ary April 2023 GAPDH (Cell Signaling, 2118S) Antibodies used for iCLIP GFP polyclonal Antibody - Thermo Fisher A6455 LIN28A polyclonal Antibody - Cell signaling #3978 PABPC1 - Abcam ab21060 and Proteintech 10970-1-AP PABPC4 - Proteintech 14960-1-AP Validation Antibodies used for Western blotting and cell immunoflourescence: LIN28A (A177 Cell Signaling). .. An antibody routinely used as a first choice for LIN28A immunoprecipitations, Western Blots and immunoflourescence: Product website outlines 42 relevant citations (https://www.cellsignal.co.uk/products/primary-antibodies/ lin28a-a177-antibody/3978?N=0+4294956287&Nrpp=200&No=3200&fromPage=plp), including publications that validated the specificity of A177 LIN28A Ab when compared to a cell line not expressing LIN28A (PMID: 27992407).

    Immunofluorescence:

    Article Title: Partial reprogramming by cyclical overexpression of Yamanaka factors improves pathological phenotypes of tauopathy mouse model of human Alzheimer's disease.
    Article Snippet: Partial reprogramming induced by the controlled and cyclical overexpression of Yamanaka factors in the nervous system has so far succeeded in reversing some aging-associated phenotypes, such as improving memory function.. These promising results suggest that partial reprogramming could be a potential strategy to prevent or mitigate aging-related pathologies like tauopathies, including Alzheimer’s disease.. Here, we explore the potential of this strategy in addressing tauopathy development in the P301S mouse model. To achieve this, a new transgenic animal was created that can inducibly overexpress Yamanaka factors upon doxycycline administration and carries the Tau-P301S mutation, which leads to tauopathy development.

    Staining:

    Article Title: Basis for lineage-determining pioneer factors targeting distinct repressed chromatin states
    Article Snippet: .. Primary V5 (1:500; Invitrogen, R960-25), OCT4 (1:100; Santa Cruz Biotechnology, C-10), KLF4 (1:100; R&D Systems, AF3640), c-MYC [1:100; Cell Signaling Technology (CST), D84C12], and SOX2 (1:500; R&D Systems, AF2018) antibody staining in blocking buffer was performed at 4°C overnight. ..

    Blocking Assay:

    Article Title: Basis for lineage-determining pioneer factors targeting distinct repressed chromatin states
    Article Snippet: .. Primary V5 (1:500; Invitrogen, R960-25), OCT4 (1:100; Santa Cruz Biotechnology, C-10), KLF4 (1:100; R&D Systems, AF3640), c-MYC [1:100; Cell Signaling Technology (CST), D84C12], and SOX2 (1:500; R&D Systems, AF2018) antibody staining in blocking buffer was performed at 4°C overnight. ..

    ChIP-qPCR:

    Article Title: Promoter-centric gene regulation in drug-resistant cancer
    Article Snippet: DNA was de-crosslinked overnight at 65C and purified with the MinElute PCR purification kit (Qiagen). .. ChIP-qPCR experiments were carried out as described above with the following antibodies: KLF4 (AF3158 – R&D Systems), NYFA (C15310261 – Diagenode). .. DNA was quantified by Qubit and equal amounts (2ng) of DNA was used for sequencing library construction with the Ovation Ultralow Library System V2 (Tecan) using 15 PCR cycles according to the manufacturer’s recommendations.



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    A) Volcano plots showing <t>KLF4</t> affinity purification-mass spectrometry (KLF4 AP-MS) data from serum-starved HaCaT keratinocytes treated with FGF7 for 6 h or vehicle vs . the respective IgG control. Enriched prey proteins are located in the upper right quadrant, with KLF4 highlighted (N = 3, one-sided Student’s t -test, FDR<0-05). B) Volcano plot depicting FGF7-dependent changes in KLF4-associated proteins (KLF4 AP-MS data) under the same experimental conditions. p < 0.05 (two-sided Student’s t -test), filtering threshold ≥3 unique tryptic peptides per protein. C) Proteins, which also showed a significantly different abundance in the KLF4 interactome upon FGF7 treatment in an independent experiment. D) Schematic representation of the luciferase reporter vector harboring 4 KLF4 binding sites in the promoter. E) Luciferase activity in lysates of serum-starved HaCaT keratinocytes, stably transduced with lentiviruses including a luciferase reporter gene preceded by KLF4 response elements. Cells were treated for 8, 16 or 24 h with FGF7 or vehicle (N = 6). F) Luciferase activity in lysates of HaCaT keratinocytes, stably transduced with lentiviruses containing a luciferase reporter gene preceded by KLF4 response elements. Cells had been serum-starved and pre-treated for 2 h with the MEK1/2 inhibitor U0126 or vehicle, followed by a 6 h treatment with FGF7 or vehicle (N = 6). G) IL6 promoter cloning strategy showing the deletion of two KLF4 binding sites (BS) and the insertion of the IL6 promoter fragment into the firefly luciferase lentiviral vector. H) Luciferase activity in lysates of HaCaT keratinocytes, stably transduced with lentiviruses containing the IL6 promoter fragment with or without KLF4 BS in front of a luciferase gene. Cells were serum-starved and treated for 6 h with FGF7 or vehicle (N = 6). I) Luciferase activity in lysates of serum-starved HaCaT keratinocytes, stably transduced with lentiviruses containing the IL6 promoter fragment with or without KLF4 BS in front of a luciferase gene. Cells had been serum-starved and pre-treated for 3 h with FGF7 or vehicle and incubated for 6 h with poly(I:C), TNFα, or vehicle (N = 6). Data information: Graphs show mean and SD. Non-significant (ns), *P < 0.05, **P < 0.01; ***P < 0.001; ****P < 0.0001 (Mann-Whitney U test (D, G; normalized to respective control), or 2-way ANOVA with Bonferroni’s multiple comparisons test (E; H).
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    Image Search Results


    A) Volcano plots showing KLF4 affinity purification-mass spectrometry (KLF4 AP-MS) data from serum-starved HaCaT keratinocytes treated with FGF7 for 6 h or vehicle vs . the respective IgG control. Enriched prey proteins are located in the upper right quadrant, with KLF4 highlighted (N = 3, one-sided Student’s t -test, FDR<0-05). B) Volcano plot depicting FGF7-dependent changes in KLF4-associated proteins (KLF4 AP-MS data) under the same experimental conditions. p < 0.05 (two-sided Student’s t -test), filtering threshold ≥3 unique tryptic peptides per protein. C) Proteins, which also showed a significantly different abundance in the KLF4 interactome upon FGF7 treatment in an independent experiment. D) Schematic representation of the luciferase reporter vector harboring 4 KLF4 binding sites in the promoter. E) Luciferase activity in lysates of serum-starved HaCaT keratinocytes, stably transduced with lentiviruses including a luciferase reporter gene preceded by KLF4 response elements. Cells were treated for 8, 16 or 24 h with FGF7 or vehicle (N = 6). F) Luciferase activity in lysates of HaCaT keratinocytes, stably transduced with lentiviruses containing a luciferase reporter gene preceded by KLF4 response elements. Cells had been serum-starved and pre-treated for 2 h with the MEK1/2 inhibitor U0126 or vehicle, followed by a 6 h treatment with FGF7 or vehicle (N = 6). G) IL6 promoter cloning strategy showing the deletion of two KLF4 binding sites (BS) and the insertion of the IL6 promoter fragment into the firefly luciferase lentiviral vector. H) Luciferase activity in lysates of HaCaT keratinocytes, stably transduced with lentiviruses containing the IL6 promoter fragment with or without KLF4 BS in front of a luciferase gene. Cells were serum-starved and treated for 6 h with FGF7 or vehicle (N = 6). I) Luciferase activity in lysates of serum-starved HaCaT keratinocytes, stably transduced with lentiviruses containing the IL6 promoter fragment with or without KLF4 BS in front of a luciferase gene. Cells had been serum-starved and pre-treated for 3 h with FGF7 or vehicle and incubated for 6 h with poly(I:C), TNFα, or vehicle (N = 6). Data information: Graphs show mean and SD. Non-significant (ns), *P < 0.05, **P < 0.01; ***P < 0.001; ****P < 0.0001 (Mann-Whitney U test (D, G; normalized to respective control), or 2-way ANOVA with Bonferroni’s multiple comparisons test (E; H).

    Journal: bioRxiv

    Article Title: An FGF7-FGFR2-KLF4 feedback loop sustains anti-inflammatory signaling in epithelial cells

    doi: 10.64898/2026.03.20.711763

    Figure Lengend Snippet: A) Volcano plots showing KLF4 affinity purification-mass spectrometry (KLF4 AP-MS) data from serum-starved HaCaT keratinocytes treated with FGF7 for 6 h or vehicle vs . the respective IgG control. Enriched prey proteins are located in the upper right quadrant, with KLF4 highlighted (N = 3, one-sided Student’s t -test, FDR<0-05). B) Volcano plot depicting FGF7-dependent changes in KLF4-associated proteins (KLF4 AP-MS data) under the same experimental conditions. p < 0.05 (two-sided Student’s t -test), filtering threshold ≥3 unique tryptic peptides per protein. C) Proteins, which also showed a significantly different abundance in the KLF4 interactome upon FGF7 treatment in an independent experiment. D) Schematic representation of the luciferase reporter vector harboring 4 KLF4 binding sites in the promoter. E) Luciferase activity in lysates of serum-starved HaCaT keratinocytes, stably transduced with lentiviruses including a luciferase reporter gene preceded by KLF4 response elements. Cells were treated for 8, 16 or 24 h with FGF7 or vehicle (N = 6). F) Luciferase activity in lysates of HaCaT keratinocytes, stably transduced with lentiviruses containing a luciferase reporter gene preceded by KLF4 response elements. Cells had been serum-starved and pre-treated for 2 h with the MEK1/2 inhibitor U0126 or vehicle, followed by a 6 h treatment with FGF7 or vehicle (N = 6). G) IL6 promoter cloning strategy showing the deletion of two KLF4 binding sites (BS) and the insertion of the IL6 promoter fragment into the firefly luciferase lentiviral vector. H) Luciferase activity in lysates of HaCaT keratinocytes, stably transduced with lentiviruses containing the IL6 promoter fragment with or without KLF4 BS in front of a luciferase gene. Cells were serum-starved and treated for 6 h with FGF7 or vehicle (N = 6). I) Luciferase activity in lysates of serum-starved HaCaT keratinocytes, stably transduced with lentiviruses containing the IL6 promoter fragment with or without KLF4 BS in front of a luciferase gene. Cells had been serum-starved and pre-treated for 3 h with FGF7 or vehicle and incubated for 6 h with poly(I:C), TNFα, or vehicle (N = 6). Data information: Graphs show mean and SD. Non-significant (ns), *P < 0.05, **P < 0.01; ***P < 0.001; ****P < 0.0001 (Mann-Whitney U test (D, G; normalized to respective control), or 2-way ANOVA with Bonferroni’s multiple comparisons test (E; H).

    Article Snippet: The beads were resuspended in 80 μl binding buffer, to which 9.21 μg of KLF4 antibody (Cell Signaling) or 9.21 μg of rabbit control IgG antibody (Merck, Darmstadt, Germany) was added.

    Techniques: Affinity Purification, Mass Spectrometry, Protein-Protein interactions, Control, Luciferase, Plasmid Preparation, Binding Assay, Activity Assay, Stable Transfection, Transduction, Cloning, Incubation, MANN-WHITNEY

    A) ChIP-seq data from the Gene Transcription Regulation Database (GTRD) showing the number of KLF4 binding sites (BS) in the promoter regions (−100 to +10 bp relative to the TSS) of FGF7-suppressed genes. B) RT-qPCR for IL6, RSAD2, ISG20 relative to RPL27 using RNA from serum-starved HaCaT keratinocytes or HPKs, which had been transfected with scrambled (scr) or KLF4 siRNA (N = 6; HPKs from two donors). C) Luciferase activity in lysates of HaCaT keratinocytes, stably transduced with lentiviruses containing the IL6 promoter fragment with or without KLF4 binding sites in front of a luciferase gene. Cells had been transfected with scrambled (scr) or KLF4 siRNA, serum-starved, and treated for 6 h with FGF7 or vehicle (N = 6). Data information: Graphs show mean and SD. Non-significant (ns), **P < 0.01, ****P < 0.0001 (Mann-Whitney U test (B, C)).

    Journal: bioRxiv

    Article Title: An FGF7-FGFR2-KLF4 feedback loop sustains anti-inflammatory signaling in epithelial cells

    doi: 10.64898/2026.03.20.711763

    Figure Lengend Snippet: A) ChIP-seq data from the Gene Transcription Regulation Database (GTRD) showing the number of KLF4 binding sites (BS) in the promoter regions (−100 to +10 bp relative to the TSS) of FGF7-suppressed genes. B) RT-qPCR for IL6, RSAD2, ISG20 relative to RPL27 using RNA from serum-starved HaCaT keratinocytes or HPKs, which had been transfected with scrambled (scr) or KLF4 siRNA (N = 6; HPKs from two donors). C) Luciferase activity in lysates of HaCaT keratinocytes, stably transduced with lentiviruses containing the IL6 promoter fragment with or without KLF4 binding sites in front of a luciferase gene. Cells had been transfected with scrambled (scr) or KLF4 siRNA, serum-starved, and treated for 6 h with FGF7 or vehicle (N = 6). Data information: Graphs show mean and SD. Non-significant (ns), **P < 0.01, ****P < 0.0001 (Mann-Whitney U test (B, C)).

    Article Snippet: The beads were resuspended in 80 μl binding buffer, to which 9.21 μg of KLF4 antibody (Cell Signaling) or 9.21 μg of rabbit control IgG antibody (Merck, Darmstadt, Germany) was added.

    Techniques: ChIP-sequencing, Binding Assay, Quantitative RT-PCR, Transfection, Luciferase, Activity Assay, Stable Transfection, Transduction, MANN-WHITNEY

    A) Western blot of lysates from serum-starved HaCaT keratinocytes, transfected with scrambled (scr) or KLF4 siRNA mix and treated at 48 h post transfection with FGF7 or vehicle for 15 min. Graphs show densitometric quantification of KLF4, FGFR2 and total FRS2α band intensities normalized to the intensity of α-tubulin (upper panel) or p-FRS2α/FRS2α and p-ERK1/2/total ERK1/2 ratios (lower panels) (N = 3). B) RT-qPCR for DUSP6 and INHBA relative to RPL27 using RNA from serum-starved HaCaT keratinocytes, transfected with scr or KLF4 siRNA and treated at 48 h post transfection with FGF7 or vehicle for 6 h (N = 3). C, D) RT-qPCR for FGFR2 and FRS2A using RNA from serum-starved HaCaT keratinocytes or HPKs, transfected with scr or KLF4 siRNA (N = 3-6; HPKs from two donors). E) ChIP-seq data from the GTRD showing the number of KLF4 binding sites (BS) in the promoter regions (−500/−100 to +10/+50 bp relative to the TSS) of the FRS2A and FGFR1 - FGFR4 genes. F) RT-qPCR for FGFR2 and FRS2A using RNA from serum-starved HaCaT keratinocytes, incubated with membrane-permeable KLF4-TAT, FITC-TAT, or vehicle for 8 h (N = 6). Data information: Graphs show mean and standard deviation (SD). Non-significant (ns), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (Student’s t-test (A, normalized to respective control; C, D), one-way ANOVA with Bonferroni’s multiple comparisons test (F) or 2-way ANOVA with Bonferroni’s multiple comparisons test (B)).

    Journal: bioRxiv

    Article Title: An FGF7-FGFR2-KLF4 feedback loop sustains anti-inflammatory signaling in epithelial cells

    doi: 10.64898/2026.03.20.711763

    Figure Lengend Snippet: A) Western blot of lysates from serum-starved HaCaT keratinocytes, transfected with scrambled (scr) or KLF4 siRNA mix and treated at 48 h post transfection with FGF7 or vehicle for 15 min. Graphs show densitometric quantification of KLF4, FGFR2 and total FRS2α band intensities normalized to the intensity of α-tubulin (upper panel) or p-FRS2α/FRS2α and p-ERK1/2/total ERK1/2 ratios (lower panels) (N = 3). B) RT-qPCR for DUSP6 and INHBA relative to RPL27 using RNA from serum-starved HaCaT keratinocytes, transfected with scr or KLF4 siRNA and treated at 48 h post transfection with FGF7 or vehicle for 6 h (N = 3). C, D) RT-qPCR for FGFR2 and FRS2A using RNA from serum-starved HaCaT keratinocytes or HPKs, transfected with scr or KLF4 siRNA (N = 3-6; HPKs from two donors). E) ChIP-seq data from the GTRD showing the number of KLF4 binding sites (BS) in the promoter regions (−500/−100 to +10/+50 bp relative to the TSS) of the FRS2A and FGFR1 - FGFR4 genes. F) RT-qPCR for FGFR2 and FRS2A using RNA from serum-starved HaCaT keratinocytes, incubated with membrane-permeable KLF4-TAT, FITC-TAT, or vehicle for 8 h (N = 6). Data information: Graphs show mean and standard deviation (SD). Non-significant (ns), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (Student’s t-test (A, normalized to respective control; C, D), one-way ANOVA with Bonferroni’s multiple comparisons test (F) or 2-way ANOVA with Bonferroni’s multiple comparisons test (B)).

    Article Snippet: The beads were resuspended in 80 μl binding buffer, to which 9.21 μg of KLF4 antibody (Cell Signaling) or 9.21 μg of rabbit control IgG antibody (Merck, Darmstadt, Germany) was added.

    Techniques: Western Blot, Transfection, Quantitative RT-PCR, ChIP-sequencing, Binding Assay, Incubation, Membrane, Standard Deviation, Control

    A) Scheme depicting the FGF7–FGFR2b-ERK1/2-KLF4 signaling axis and its effect on inflammatory gene expression keratinocytes. The icons were obtained from BioRender. Werner, S. (2026) https://BioRender.com/kreppyv . B) Representative immunofluorescence stainings of normal human skin for FGFR2 (purple) and KLF4 (green). Scale bar: 20 μm.

    Journal: bioRxiv

    Article Title: An FGF7-FGFR2-KLF4 feedback loop sustains anti-inflammatory signaling in epithelial cells

    doi: 10.64898/2026.03.20.711763

    Figure Lengend Snippet: A) Scheme depicting the FGF7–FGFR2b-ERK1/2-KLF4 signaling axis and its effect on inflammatory gene expression keratinocytes. The icons were obtained from BioRender. Werner, S. (2026) https://BioRender.com/kreppyv . B) Representative immunofluorescence stainings of normal human skin for FGFR2 (purple) and KLF4 (green). Scale bar: 20 μm.

    Article Snippet: The beads were resuspended in 80 μl binding buffer, to which 9.21 μg of KLF4 antibody (Cell Signaling) or 9.21 μg of rabbit control IgG antibody (Merck, Darmstadt, Germany) was added.

    Techniques: Gene Expression, Immunofluorescence