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Boehringer Mannheim klenow dna polymerase
Klenow Dna Polymerase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/klenow dna polymerase/product/Boehringer Mannheim
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
klenow dna polymerase - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Transfection:

Article Title: Mutations in Exon 11 of c-Kit Occur Preferentially in Malignant versus Benign Gastrointestinal Stromal Tumors and Do Not Occur in Leiomyomas or Leiomyosarcomas
Article Snippet: .. The PCR products were purified from the gels, treated with Klenow DNA polymerase (Boehringer Mannheim, Indianapolis, IN), blunt end-ligated into pBSK+ phagemid (Stratagene, La Jolla, CA), and transfected into DH5α-competent cells (Gibco-BRL, Gaithersburg, MD). .. White colonies obtained from the plating on IPTG/X-gal selective medium (Gibco-BRL) were picked and screened by PCR as described above to confirm the presence of the appropriate inserts.

Labeling:

Article Title: Restriction Fragment Length Polymorphism Analysis of Ribosomal DNA Intergenic Regions Is Useful for Differentiating Strains of Trichophyton mentagrophytes
Article Snippet: .. The DNA fragments were denatured by boiling; random hexanucleotide primers, deoxynucleoside triphosphates, Klenow DNA polymerase, and digoxigenin (DIG)-labeled dUTP were added; and the fragments were labeled according to the instructions of the manufacturer of the DIG DNA Labeling kit (Boehringer Mannheim UK Ltd., Lewes, United Kingdom). .. The blotted membranes were hybridized with the probe for 18 h at 65°C, followed by two careful washes, and then the signals were detected with anti-DIG-alkaline phosphatase conjugate and by an enzyme-catalyzed color reaction according to instructions of the manufacturer of the DIG Nucleic Acid Detection kit (Boehringer Mannheim UK Ltd.).

Purification:

Article Title: Mutations in Exon 11 of c-Kit Occur Preferentially in Malignant versus Benign Gastrointestinal Stromal Tumors and Do Not Occur in Leiomyomas or Leiomyosarcomas
Article Snippet: .. The PCR products were purified from the gels, treated with Klenow DNA polymerase (Boehringer Mannheim, Indianapolis, IN), blunt end-ligated into pBSK+ phagemid (Stratagene, La Jolla, CA), and transfected into DH5α-competent cells (Gibco-BRL, Gaithersburg, MD). .. White colonies obtained from the plating on IPTG/X-gal selective medium (Gibco-BRL) were picked and screened by PCR as described above to confirm the presence of the appropriate inserts.

other:

Article Title: Characterization of Dihydrofolate Reductase Genes from Trimethoprim-Susceptible and Trimethoprim-Resistant Strains of Enterococcus faecalis
Article Snippet: Klenow DNA polymerase was obtained from Boehringer Mannheim (Indianapolis, Ind.), shrimp alkaline phosphatase was obtained from United States Biochemicals (Cleveland, Ohio), and Incert agarose and SeaKem Gold agarose were provided by FMC Bioproducts (Rockland, Maine).

DNA Labeling:

Article Title: Restriction Fragment Length Polymorphism Analysis of Ribosomal DNA Intergenic Regions Is Useful for Differentiating Strains of Trichophyton mentagrophytes
Article Snippet: .. The DNA fragments were denatured by boiling; random hexanucleotide primers, deoxynucleoside triphosphates, Klenow DNA polymerase, and digoxigenin (DIG)-labeled dUTP were added; and the fragments were labeled according to the instructions of the manufacturer of the DIG DNA Labeling kit (Boehringer Mannheim UK Ltd., Lewes, United Kingdom). .. The blotted membranes were hybridized with the probe for 18 h at 65°C, followed by two careful washes, and then the signals were detected with anti-DIG-alkaline phosphatase conjugate and by an enzyme-catalyzed color reaction according to instructions of the manufacturer of the DIG Nucleic Acid Detection kit (Boehringer Mannheim UK Ltd.).

Polymerase Chain Reaction:

Article Title: Mutations in Exon 11 of c-Kit Occur Preferentially in Malignant versus Benign Gastrointestinal Stromal Tumors and Do Not Occur in Leiomyomas or Leiomyosarcomas
Article Snippet: .. The PCR products were purified from the gels, treated with Klenow DNA polymerase (Boehringer Mannheim, Indianapolis, IN), blunt end-ligated into pBSK+ phagemid (Stratagene, La Jolla, CA), and transfected into DH5α-competent cells (Gibco-BRL, Gaithersburg, MD). .. White colonies obtained from the plating on IPTG/X-gal selective medium (Gibco-BRL) were picked and screened by PCR as described above to confirm the presence of the appropriate inserts.

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  • 92
    Boehringer Mannheim klenow fragment
    The effect of Ku on the 3′→5′ exonuclease activity of WRN, <t>Klenow</t> and exo <t>III</t> on a substrate containing 8-oxoA. DNA substrate containing 8-oxoA was incubated without enzyme (–enzyme) or with WRN (180 fmol), Klenow (2 U) or exo III (1 U) in the absence or presence of Ku (64 fmol) at 37°C for 1 h. The labeled reaction products were analyzed and visualized as described earlier.
    Klenow Fragment, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/klenow fragment/product/Boehringer Mannheim
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    klenow fragment - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    88
    Boehringer Mannheim klenow dna polymerase i
    Extension synthesis by <t>Klenow</t> <t>DNA</t> <t>polymerase-I</t> on single-stranded DNA breaks. The AP site in 32 P-DS oligomer D was treated with (lanes 2–5) or without (lane 1) E. coli ExoIII ( A ). The reaction mixtures were then incubated either with buffer (lanes 1–3) or dNTP (40 μM) plus endonuclease-free Klenow DNA polymerase-I (lanes 4 and 5 for two different lots of enzymes) at 37°C for 5 min. The oh8dG removal in 32 P-DS oligomer Z was treated with (lanes 2–6) or without (lane 1) E. coli Fpg protein at 37°C for 10 min ( B ). The PO 4 terminus in 32 P-DS oligomer Z was incubated with (lanes 3, 5, 6) or without (lanes 1, 2, 4) E. coli Exo III at 37°C for 10 min before primer extension using Klenow DNA polymerase-I. The reaction was stopped by heating and further analyzed in sequencing PAGE (10%). +, Enzyme added, -, no enzyme (buffer only). Note that DNA polymerase-I could not perform primer extension synthesis (lane 4) unless the Fpg-protein products (3′-PO 4 termini) were pretreated with ExoIII (lanes 5 and 6, for two different lots of DNA pol-I).
    Klenow Dna Polymerase I, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/klenow dna polymerase i/product/Boehringer Mannheim
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    klenow dna polymerase i - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    90
    Boehringer Mannheim escherichia coli dna polymerase i
    Neutralization of <t>DNA</t> polymerase activity by an anti-integrase MAb. Dilutions of a mouse ascites fluid containing MAb 35 were prepared in phosphate-buffered saline, and 1 μl of the diluted antibody was added to each 25-μl reaction mixture. Half-filled triangles represent reactions with integrase; open triangles represent reactions with E. coli DNA <t>polymerase</t> I (Klenow fragment).
    Escherichia Coli Dna Polymerase I, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli dna polymerase i/product/Boehringer Mannheim
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    escherichia coli dna polymerase i - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    Image Search Results


    The effect of Ku on the 3′→5′ exonuclease activity of WRN, Klenow and exo III on a substrate containing 8-oxoA. DNA substrate containing 8-oxoA was incubated without enzyme (–enzyme) or with WRN (180 fmol), Klenow (2 U) or exo III (1 U) in the absence or presence of Ku (64 fmol) at 37°C for 1 h. The labeled reaction products were analyzed and visualized as described earlier.

    Journal: Nucleic Acids Research

    Article Title: A functional interaction of Ku with Werner exonuclease facilitates digestion of damaged DNA

    doi:

    Figure Lengend Snippet: The effect of Ku on the 3′→5′ exonuclease activity of WRN, Klenow and exo III on a substrate containing 8-oxoA. DNA substrate containing 8-oxoA was incubated without enzyme (–enzyme) or with WRN (180 fmol), Klenow (2 U) or exo III (1 U) in the absence or presence of Ku (64 fmol) at 37°C for 1 h. The labeled reaction products were analyzed and visualized as described earlier.

    Article Snippet: Exonuclease III (exo III) and the Klenow fragment of Escherichia coli were purchased from Boehringer Mannheim and T4 polynucleotide kinase was from New England Biolabs (Beverly, MA).

    Techniques: Activity Assay, Incubation, Labeling

    Extension synthesis by Klenow DNA polymerase-I on single-stranded DNA breaks. The AP site in 32 P-DS oligomer D was treated with (lanes 2–5) or without (lane 1) E. coli ExoIII ( A ). The reaction mixtures were then incubated either with buffer (lanes 1–3) or dNTP (40 μM) plus endonuclease-free Klenow DNA polymerase-I (lanes 4 and 5 for two different lots of enzymes) at 37°C for 5 min. The oh8dG removal in 32 P-DS oligomer Z was treated with (lanes 2–6) or without (lane 1) E. coli Fpg protein at 37°C for 10 min ( B ). The PO 4 terminus in 32 P-DS oligomer Z was incubated with (lanes 3, 5, 6) or without (lanes 1, 2, 4) E. coli Exo III at 37°C for 10 min before primer extension using Klenow DNA polymerase-I. The reaction was stopped by heating and further analyzed in sequencing PAGE (10%). +, Enzyme added, -, no enzyme (buffer only). Note that DNA polymerase-I could not perform primer extension synthesis (lane 4) unless the Fpg-protein products (3′-PO 4 termini) were pretreated with ExoIII (lanes 5 and 6, for two different lots of DNA pol-I).

    Journal: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: In situ detection of AP sites and DNA strand breaks bearing 3?-phosphate termini in ischemic mouse brain

    doi:

    Figure Lengend Snippet: Extension synthesis by Klenow DNA polymerase-I on single-stranded DNA breaks. The AP site in 32 P-DS oligomer D was treated with (lanes 2–5) or without (lane 1) E. coli ExoIII ( A ). The reaction mixtures were then incubated either with buffer (lanes 1–3) or dNTP (40 μM) plus endonuclease-free Klenow DNA polymerase-I (lanes 4 and 5 for two different lots of enzymes) at 37°C for 5 min. The oh8dG removal in 32 P-DS oligomer Z was treated with (lanes 2–6) or without (lane 1) E. coli Fpg protein at 37°C for 10 min ( B ). The PO 4 terminus in 32 P-DS oligomer Z was incubated with (lanes 3, 5, 6) or without (lanes 1, 2, 4) E. coli Exo III at 37°C for 10 min before primer extension using Klenow DNA polymerase-I. The reaction was stopped by heating and further analyzed in sequencing PAGE (10%). +, Enzyme added, -, no enzyme (buffer only). Note that DNA polymerase-I could not perform primer extension synthesis (lane 4) unless the Fpg-protein products (3′-PO 4 termini) were pretreated with ExoIII (lanes 5 and 6, for two different lots of DNA pol-I).

    Article Snippet: 32 P-DS oligomer Z after Fpg protein without ExoIII was not used by Klenow DNA polymerase-I, indicating that the 3′-PO4 termini in DS oligomer Z generated by E. coli Fpg protein could not be used for extension by Klenow DNA polymerase I.

    Techniques: Incubation, Sequencing, Polyacrylamide Gel Electrophoresis

    In situ EXOSS detection using E. coli ExoIII and Klenow DNA polymerase-I. Cerebral cortices from animals with no FbIR ( A ), or FbIR of 90/0 ( B ), 90/15 ( C , D ). All panels were from samples treated with ExoIII, except panel D , before incorporation of dig-dUTP using Klenow DNA polymerase-I. The green fluorescence represents signs of EXOSS, and the orange signal indicates nuclei as counter-stained using PI. Bar = 50 μm.

    Journal: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: In situ detection of AP sites and DNA strand breaks bearing 3?-phosphate termini in ischemic mouse brain

    doi:

    Figure Lengend Snippet: In situ EXOSS detection using E. coli ExoIII and Klenow DNA polymerase-I. Cerebral cortices from animals with no FbIR ( A ), or FbIR of 90/0 ( B ), 90/15 ( C , D ). All panels were from samples treated with ExoIII, except panel D , before incorporation of dig-dUTP using Klenow DNA polymerase-I. The green fluorescence represents signs of EXOSS, and the orange signal indicates nuclei as counter-stained using PI. Bar = 50 μm.

    Article Snippet: 32 P-DS oligomer Z after Fpg protein without ExoIII was not used by Klenow DNA polymerase-I, indicating that the 3′-PO4 termini in DS oligomer Z generated by E. coli Fpg protein could not be used for extension by Klenow DNA polymerase I.

    Techniques: In Situ, Fluorescence, Staining

    Neutralization of DNA polymerase activity by an anti-integrase MAb. Dilutions of a mouse ascites fluid containing MAb 35 were prepared in phosphate-buffered saline, and 1 μl of the diluted antibody was added to each 25-μl reaction mixture. Half-filled triangles represent reactions with integrase; open triangles represent reactions with E. coli DNA polymerase I (Klenow fragment).

    Journal: Journal of Virology

    Article Title: Efficient Gap Repair Catalyzed In Vitro by an Intrinsic DNA Polymerase Activity of Human Immunodeficiency Virus Type 1 Integrase

    doi:

    Figure Lengend Snippet: Neutralization of DNA polymerase activity by an anti-integrase MAb. Dilutions of a mouse ascites fluid containing MAb 35 were prepared in phosphate-buffered saline, and 1 μl of the diluted antibody was added to each 25-μl reaction mixture. Half-filled triangles represent reactions with integrase; open triangles represent reactions with E. coli DNA polymerase I (Klenow fragment).

    Article Snippet: CHAPS {3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate} and Escherichia coli DNA polymerase I (Klenow fragment) and the holoenzyme were from Boehringer Mannheim.

    Techniques: Neutralization, Activity Assay