Journal: International Journal of Microbiology

Article Title: Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

doi: 10.1155/2020/9483670

Figure Lengend Snippet: Minimum inhibitory concentration of M. radiotolerans MAMP 4754 crude extract ( μ g/mL). Kanamycin, Ampicillin, and Ketoconazole were used as positive controls (1 mg/mL). Data were reported as mean values of the crude extract tested in triplicate. Statistical analysis was performed using ANOVA and the differences were considered to be significant at p < 0.05.

Article Snippet: The MICs were recorded at 250 μ g/mL for B. subtilis ATCC 19659, B. cereus ATCC 1076, E. coli ATCC1053, and 62.5 μ g/mL for K. oxytoca ATCC 13182 and M. smegmatis ATCC 21293, while an IC 50 of 5.65 μ g/mL was recorded with the DPPH assay.

Techniques: Concentration Assay

Journal: Journal of AOAC International

Article Title: 3M ™ Petrifilm ™ Rapid Aerobic Count (RAC) Plate Method for the Enumeration of Aerobic Bacteria on Selected Surfaces: AOAC Official Method SM 2015.13

doi: 10.1093/jaoacint/qsac122

Figure Lengend Snippet: Matrix study: 3M Petrifilm RAC Plate versus FDA BAM Ch. 3 results

Article Snippet: The organisms used in the matrix study were obtained from the American Type Culture Collection (ATCC, Manassas, VA): Listeria innocua ATCC 33090 (source: cow brain), Klebsiella (formerly Enterobacter ) aerogenes ATCC 35029 (origin not available), and Klebsiella oxytoca ATCC 43165 (source: clinical isolate).

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Propolis: A Complex Natural Product with a Plethora of Biological Activities That Can Be Explored for Drug Development

doi: 10.1155/2015/206439

Figure Lengend Snippet: Antibacterial activity of propolis and its chemical constituents.

Article Snippet: France , European propolis/ Populus nigra , Dichloromethane extract , Gram-negative: 7 Acinetobacter baumannii (RCH, SAN008, 12, AYE, CIP7034, 107292, and 5377), 2 Escherichia coli (ATCC 25922 and a clinical isolate), 3 Pseudomonas aeruginosa (ATCC 27853 and two clinical isolates), and 4 clinical isolates of Enterobacter cloacae , E. aerogenes , Klebsiella oxytoca , and Salmonella enteritidis (phage type 4) Gram-negative: 13 Staphylococcus aureus (ATCC 25923, six methicillin-susceptible clinical isolates, and six methicillin-resistant clinical isolates), 2 clinical isolates of S. epidermidis (methiS and methiR), 3 clinical isolates of Enterococcus faecalis and 1 E. faecium , and 1 clinical isolate of Corynebacterium striatum , Influencing the Gram-positive bacteria viability specially S. aureus and several of its methicillin-resistant and methicillin-susceptible , [ ] .

Techniques: Activity Assay, Permeability, Inhibition, Isolation, Mouse Assay, Infection, Blocking Assay

Journal: PLoS ONE

Article Title: Cytotoxic and Pathogenic Properties of Klebsiella oxytoca Isolated from Laboratory Animals

doi: 10.1371/journal.pone.0100542

Figure Lengend Snippet: Sources and origin of Klebsiella oxytoca used in this study.

Article Snippet: In addition, a non-toxigenic K. oxytoca strain, ATCC 13182 , was obtained from American Type Culture Collection (ATCC) and used as a control.

Techniques: Diagnostic Assay

Journal: PLoS ONE

Article Title: Cytotoxic and Pathogenic Properties of Klebsiella oxytoca Isolated from Laboratory Animals

doi: 10.1371/journal.pone.0100542

Figure Lengend Snippet: Antimicrobial resistance of K. oxytoca.

Article Snippet: In addition, a non-toxigenic K. oxytoca strain, ATCC 13182 , was obtained from American Type Culture Collection (ATCC) and used as a control.

Techniques:

Journal: PLoS ONE

Article Title: Cytotoxic and Pathogenic Properties of Klebsiella oxytoca Isolated from Laboratory Animals

doi: 10.1371/journal.pone.0100542

Figure Lengend Snippet: Cytotoxin of K. oxytoca varies with different growth conditions.

Article Snippet: In addition, a non-toxigenic K. oxytoca strain, ATCC 13182 , was obtained from American Type Culture Collection (ATCC) and used as a control.

Techniques:

Journal: PLoS ONE

Article Title: Cytotoxic and Pathogenic Properties of Klebsiella oxytoca Isolated from Laboratory Animals

doi: 10.1371/journal.pone.0100542

Figure Lengend Snippet: HEp-2 cell culture inoculated with K. oxytoca supernatant and media control at 4, 10, and 30× magnifications. A) Media control showing normal morphology of cells stained with Diff-quick after 48 hours of incubation. B) K. oxytoca , ATCC 13182, isolate negative for cytotoxin production showing normal cell morphology. C) K. oxytoca isolate, 09-7231-1, positive for cytotoxin production showing abnormal cell morphology, decreased concentration of attached cells, small round cells, and multinucleated cells.

Article Snippet: In addition, a non-toxigenic K. oxytoca strain, ATCC 13182 , was obtained from American Type Culture Collection (ATCC) and used as a control.

Techniques: Cell Culture, Staining, Diff-Quik, Incubation, Concentration Assay

Journal: PLoS ONE

Article Title: Cytotoxic and Pathogenic Properties of Klebsiella oxytoca Isolated from Laboratory Animals

doi: 10.1371/journal.pone.0100542

Figure Lengend Snippet: K. oxytoca , 09-7231-1, was cultured in A) LB; B) TSB; and C) HBI liquid media. At various time points, the supernatant was collected and used in the cytotoxicity assay using HEp-2 cells. TSB media has the strongest cytotoxin induction capability compared to LB and HBI media. The degree of cytoxicity is indirectly proportional to the level of monolayer confluency i.e., the higher % the confluency, the lower the cytotoxity and vice versa.

Article Snippet: In addition, a non-toxigenic K. oxytoca strain, ATCC 13182 , was obtained from American Type Culture Collection (ATCC) and used as a control.

Techniques: Cell Culture, Cytotoxicity Assay

Journal: PLoS ONE

Article Title: Cytotoxic and Pathogenic Properties of Klebsiella oxytoca Isolated from Laboratory Animals

doi: 10.1371/journal.pone.0100542

Figure Lengend Snippet: HEp-2 cells treated with A) media containing only casein extract (Tryptone; control); B) supernatant of K. oxytoca , 09-7231-1, grown in media containing only casein extract; C) media containing only soy extract (Soytone); D) supernatant of K. oxytoca , 09-7231-1, grown in the media containing only soy extract.

Article Snippet: In addition, a non-toxigenic K. oxytoca strain, ATCC 13182 , was obtained from American Type Culture Collection (ATCC) and used as a control.

Techniques:

Journal: PLoS ONE

Article Title: Cytotoxic and Pathogenic Properties of Klebsiella oxytoca Isolated from Laboratory Animals

doi: 10.1371/journal.pone.0100542

Figure Lengend Snippet: The arrow beneath the bar shows the region in the reference protein to which the corresponding K. Oxytoca protein aligns from the BLAST analysis.

Article Snippet: In addition, a non-toxigenic K. oxytoca strain, ATCC 13182 , was obtained from American Type Culture Collection (ATCC) and used as a control.

Techniques: