antibodies against kir6 1  (Alomone Labs)


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    Structured Review

    Alomone Labs antibodies against kir6 1
    Nico ameliorated LPS-induced ALI and inflammation. (a) Nico increased LPS-induced <t>Kir6.1</t> and Kir6.2 downregulation in the lung. (b, c) Lung sections stained with H E showed severe injury in the LPS group which was attenuated by Nico pretreatment. The data revealed a high score for the LPS-treated group which was decreased in the Nico-pretreated group. (d) Nico pretreatment significantly reduced LPS-induced protein leakage in BALF. (e, f) Nico alleviated LPS-induced increments of MPO activities in BALF and lung homogenate. (g, h) Nico prevented the production of TNF- α and IL-1 β in lung homogenate. Data were shown as mean ± SEM ( n = 6 − 8). Statistically significant differences: ∗ P
    Antibodies Against Kir6 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against kir6 1/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against kir6 1 - by Bioz Stars, 2022-01
    90/100 stars

    Images

    1) Product Images from "Nicorandil Attenuates LPS-Induced Acute Lung Injury by Pulmonary Endothelial Cell Protection via NF-κB and MAPK Pathways"

    Article Title: Nicorandil Attenuates LPS-Induced Acute Lung Injury by Pulmonary Endothelial Cell Protection via NF-κB and MAPK Pathways

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2019/4957646

    Nico ameliorated LPS-induced ALI and inflammation. (a) Nico increased LPS-induced Kir6.1 and Kir6.2 downregulation in the lung. (b, c) Lung sections stained with H E showed severe injury in the LPS group which was attenuated by Nico pretreatment. The data revealed a high score for the LPS-treated group which was decreased in the Nico-pretreated group. (d) Nico pretreatment significantly reduced LPS-induced protein leakage in BALF. (e, f) Nico alleviated LPS-induced increments of MPO activities in BALF and lung homogenate. (g, h) Nico prevented the production of TNF- α and IL-1 β in lung homogenate. Data were shown as mean ± SEM ( n = 6 − 8). Statistically significant differences: ∗ P
    Figure Legend Snippet: Nico ameliorated LPS-induced ALI and inflammation. (a) Nico increased LPS-induced Kir6.1 and Kir6.2 downregulation in the lung. (b, c) Lung sections stained with H E showed severe injury in the LPS group which was attenuated by Nico pretreatment. The data revealed a high score for the LPS-treated group which was decreased in the Nico-pretreated group. (d) Nico pretreatment significantly reduced LPS-induced protein leakage in BALF. (e, f) Nico alleviated LPS-induced increments of MPO activities in BALF and lung homogenate. (g, h) Nico prevented the production of TNF- α and IL-1 β in lung homogenate. Data were shown as mean ± SEM ( n = 6 − 8). Statistically significant differences: ∗ P

    Techniques Used: Staining

    2) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    3) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    4) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    5) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    6) Product Images from "Oral administration of the KATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis"

    Article Title: Oral administration of the KATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-8-149

    Western blotting show expression of both Kir6.1 and Kir6.2 K ATP  channel pore-forming subunits in unstimulated and LPS/IFNγ-stimulated BV-2 cells (A, left) and microglial primary cultures (A, Right). Staining for the microglial cell membrane marker CD11b (B and E) and the K ATP  channel subunits Kir 6.1 (C) or Kir 6.2 (F) showed colocalization in BV-2 microglia, indicating the expression of the K ATP  channel at the cytoplasmic membrane (D and G, white arrows) . Control: unstimulated cells; L+I: cells stimulated with LPS and IFNγ. Scale bar = 30 μm.
    Figure Legend Snippet: Western blotting show expression of both Kir6.1 and Kir6.2 K ATP channel pore-forming subunits in unstimulated and LPS/IFNγ-stimulated BV-2 cells (A, left) and microglial primary cultures (A, Right). Staining for the microglial cell membrane marker CD11b (B and E) and the K ATP channel subunits Kir 6.1 (C) or Kir 6.2 (F) showed colocalization in BV-2 microglia, indicating the expression of the K ATP channel at the cytoplasmic membrane (D and G, white arrows) . Control: unstimulated cells; L+I: cells stimulated with LPS and IFNγ. Scale bar = 30 μm.

    Techniques Used: Western Blot, Expressing, Staining, Marker

    7) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    8) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    9) Product Images from "Oral administration of the KATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis"

    Article Title: Oral administration of the KATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-8-149

    Western blotting show expression of both Kir6.1 and Kir6.2 K ATP channel pore-forming subunits in unstimulated and LPS/IFNγ-stimulated BV-2 cells (A, left) and microglial primary cultures (A, Right). Staining for the microglial cell membrane marker CD11b (B and E) and the K ATP channel subunits Kir 6.1 (C) or Kir 6.2 (F) showed colocalization in BV-2 microglia, indicating the expression of the K ATP channel at the cytoplasmic membrane (D and G, white arrows) . Control: unstimulated cells; L+I: cells stimulated with LPS and IFNγ. Scale bar = 30 μm.
    Figure Legend Snippet: Western blotting show expression of both Kir6.1 and Kir6.2 K ATP channel pore-forming subunits in unstimulated and LPS/IFNγ-stimulated BV-2 cells (A, left) and microglial primary cultures (A, Right). Staining for the microglial cell membrane marker CD11b (B and E) and the K ATP channel subunits Kir 6.1 (C) or Kir 6.2 (F) showed colocalization in BV-2 microglia, indicating the expression of the K ATP channel at the cytoplasmic membrane (D and G, white arrows) . Control: unstimulated cells; L+I: cells stimulated with LPS and IFNγ. Scale bar = 30 μm.

    Techniques Used: Western Blot, Expressing, Staining, Marker

    10) Product Images from "The ATP-sensitive K+-channel (KATP) controls early left-right patterning in Xenopus and chick embryos"

    Article Title: The ATP-sensitive K+-channel (KATP) controls early left-right patterning in Xenopus and chick embryos

    Journal: Developmental biology

    doi: 10.1016/j.ydbio.2010.07.011

    Immunolocalization of Xenopus Kir6.1 and SUR2A
    Figure Legend Snippet: Immunolocalization of Xenopus Kir6.1 and SUR2A

    Techniques Used:

    11) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    12) Product Images from "Oral administration of the KATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis"

    Article Title: Oral administration of the KATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-8-149

    Western blotting show expression of both Kir6.1 and Kir6.2 K ATP channel pore-forming subunits in unstimulated and LPS/IFNγ-stimulated BV-2 cells (A, left) and microglial primary cultures (A, Right). Staining for the microglial cell membrane marker CD11b (B and E) and the K ATP channel subunits Kir 6.1 (C) or Kir 6.2 (F) showed colocalization in BV-2 microglia, indicating the expression of the K ATP channel at the cytoplasmic membrane (D and G, white arrows) . Control: unstimulated cells; L+I: cells stimulated with LPS and IFNγ. Scale bar = 30 μm.
    Figure Legend Snippet: Western blotting show expression of both Kir6.1 and Kir6.2 K ATP channel pore-forming subunits in unstimulated and LPS/IFNγ-stimulated BV-2 cells (A, left) and microglial primary cultures (A, Right). Staining for the microglial cell membrane marker CD11b (B and E) and the K ATP channel subunits Kir 6.1 (C) or Kir 6.2 (F) showed colocalization in BV-2 microglia, indicating the expression of the K ATP channel at the cytoplasmic membrane (D and G, white arrows) . Control: unstimulated cells; L+I: cells stimulated with LPS and IFNγ. Scale bar = 30 μm.

    Techniques Used: Western Blot, Expressing, Staining, Marker

    13) Product Images from "The ATP-sensitive K+-channel (KATP) controls early left-right patterning in Xenopus and chick embryos"

    Article Title: The ATP-sensitive K+-channel (KATP) controls early left-right patterning in Xenopus and chick embryos

    Journal: Developmental biology

    doi: 10.1016/j.ydbio.2010.07.011

    Immunolocalization of Xenopus Kir6.1 and SUR2A
    Figure Legend Snippet: Immunolocalization of Xenopus Kir6.1 and SUR2A

    Techniques Used:

    14) Product Images from "Activation of ATP‐sensitive potassium channels facilitates the function of human endothelial colony‐forming cells via Ca2+/Akt/ eNOS pathway"

    Article Title: Activation of ATP‐sensitive potassium channels facilitates the function of human endothelial colony‐forming cells via Ca2+/Akt/ eNOS pathway

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.13006

    The expression of K ATP subtypes in ECFC s. ( A ) RT ‐ PCR showed the expression of Kir6.1, Kir6.2 and SUR 2b, but not SUR 2a and SUR 1 in mRNA level. HPAEC s and mouse brain were used as positive controls, water (no template) as a negative control ( n = 3). ( B ) Western blotting confirmed the expression of Kir6.1 (48 kD), Kir6.2 (44 kD) and SUR 2b (140–150 kD), but not SUR 2a (140–150 kD) and SUR 1 (175 kD), using HPAEC s and mouse brain as positive controls ( n = 3). ( C ) Confocal images showed the subcellular localization of K ATP subunits in ECFC s co‐stained with a endothelial specific marker ( CD 31 or VE ‐cadherin), revealing the extensive distribution of Kir6.1, Kir6.2 and SUR 2b. DAPI staining for nuclear labelling ( n = 3), scale bar: 20 μm. M: marker, MB : mouse brain.
    Figure Legend Snippet: The expression of K ATP subtypes in ECFC s. ( A ) RT ‐ PCR showed the expression of Kir6.1, Kir6.2 and SUR 2b, but not SUR 2a and SUR 1 in mRNA level. HPAEC s and mouse brain were used as positive controls, water (no template) as a negative control ( n = 3). ( B ) Western blotting confirmed the expression of Kir6.1 (48 kD), Kir6.2 (44 kD) and SUR 2b (140–150 kD), but not SUR 2a (140–150 kD) and SUR 1 (175 kD), using HPAEC s and mouse brain as positive controls ( n = 3). ( C ) Confocal images showed the subcellular localization of K ATP subunits in ECFC s co‐stained with a endothelial specific marker ( CD 31 or VE ‐cadherin), revealing the extensive distribution of Kir6.1, Kir6.2 and SUR 2b. DAPI staining for nuclear labelling ( n = 3), scale bar: 20 μm. M: marker, MB : mouse brain.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Negative Control, Western Blot, Staining, Marker

    15) Product Images from "Diabetes mellitus reduces the function and expression of ATP-dependent K+ channels in cardiac mitochondria"

    Article Title: Diabetes mellitus reduces the function and expression of ATP-dependent K+ channels in cardiac mitochondria

    Journal: Life sciences

    doi: 10.1016/j.lfs.2012.11.019

    Diabetes mellitus reduces Kir6.1 expression in SSM
    Figure Legend Snippet: Diabetes mellitus reduces Kir6.1 expression in SSM

    Techniques Used: Expressing

    16) Product Images from "High blood pressure associates with the remodelling of inward rectifier K+ channels in mice mesenteric vascular smooth muscle cells"

    Article Title: High blood pressure associates with the remodelling of inward rectifier K+ channels in mice mesenteric vascular smooth muscle cells

    Journal: The Journal of Physiology

    doi: 10.1113/jphysiol.2012.236190

    Expression of Kir2.1, Kir4.1, Kir6.1 and SUR2 proteins in BPN and BPH mesenteric VSMCs
    Figure Legend Snippet: Expression of Kir2.1, Kir4.1, Kir6.1 and SUR2 proteins in BPN and BPH mesenteric VSMCs

    Techniques Used: Expressing

    17) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    18) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    19) Product Images from "Role of Kir6.2 subunits of ATP-sensitive potassium channels in endotoxemia-induced cardiac dysfunction"

    Article Title: Role of Kir6.2 subunits of ATP-sensitive potassium channels in endotoxemia-induced cardiac dysfunction

    Journal: Cardiovascular Diabetology

    doi: 10.1186/1475-2840-12-75

    Unchanged Kir6.1 subunits expression in heart tissues in Kir6.2 −/− mice. Data are expressed as means ± SEM (n=3 per group).
    Figure Legend Snippet: Unchanged Kir6.1 subunits expression in heart tissues in Kir6.2 −/− mice. Data are expressed as means ± SEM (n=3 per group).

    Techniques Used: Expressing, Mouse Assay

    20) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    21) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    22) Product Images from "Role of vascular KATP channels in blood pressure variability after sinoaortic denervation in rats"

    Article Title: Role of vascular KATP channels in blood pressure variability after sinoaortic denervation in rats

    Journal: Acta Pharmacologica Sinica

    doi: 10.1038/aps.2010.195

    Representative images of Kir6.1, Kir6.2, and SUR2 expression in aorta (A–C) and mesenteric artery (D–F) from sinoaortic denervated (SAD) and sham-operated rats using laser scanning confocal microscopy. Aortic SUR2 expression was greater,
    Figure Legend Snippet: Representative images of Kir6.1, Kir6.2, and SUR2 expression in aorta (A–C) and mesenteric artery (D–F) from sinoaortic denervated (SAD) and sham-operated rats using laser scanning confocal microscopy. Aortic SUR2 expression was greater,

    Techniques Used: Expressing, Confocal Microscopy

    Expression of Kir6.1, Kir6.2 and SUR2 in both aorta and mesenteric artery in sinoaortic denervated and sham-operated rats. A and B, fold changes of mRNA level (Kir6.1, Kir6.2, and SUR2) in aorta and mesenteric artery ( n =7). C and D, fold changes of protein
    Figure Legend Snippet: Expression of Kir6.1, Kir6.2 and SUR2 in both aorta and mesenteric artery in sinoaortic denervated and sham-operated rats. A and B, fold changes of mRNA level (Kir6.1, Kir6.2, and SUR2) in aorta and mesenteric artery ( n =7). C and D, fold changes of protein

    Techniques Used: Expressing

    23) Product Images from "Role of Kir6.2 subunits of ATP-sensitive potassium channels in endotoxemia-induced cardiac dysfunction"

    Article Title: Role of Kir6.2 subunits of ATP-sensitive potassium channels in endotoxemia-induced cardiac dysfunction

    Journal: Cardiovascular Diabetology

    doi: 10.1186/1475-2840-12-75

    Unchanged Kir6.1 subunits expression in heart tissues in Kir6.2 −/− mice. Data are expressed as means ± SEM (n=3 per group).
    Figure Legend Snippet: Unchanged Kir6.1 subunits expression in heart tissues in Kir6.2 −/− mice. Data are expressed as means ± SEM (n=3 per group).

    Techniques Used: Expressing, Mouse Assay

    24) Product Images from "Is Kir6.1 a subunit of mitoKATP?"

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    Journal:

    doi: 10.1016/j.bbrc.2007.11.154

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes
    Figure Legend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Techniques Used:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody
    Figure Legend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Techniques Used:

    25) Product Images from "Activation of ATP‐sensitive potassium channels facilitates the function of human endothelial colony‐forming cells via Ca2+/Akt/ eNOS pathway"

    Article Title: Activation of ATP‐sensitive potassium channels facilitates the function of human endothelial colony‐forming cells via Ca2+/Akt/ eNOS pathway

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.13006

    The expression of K ATP subtypes in ECFC s. ( A ) RT ‐ PCR showed the expression of Kir6.1, Kir6.2 and SUR 2b, but not SUR 2a and SUR 1 in mRNA level. HPAEC s and mouse brain were used as positive controls, water (no template) as a negative control ( n = 3). ( B ) Western blotting confirmed the expression of Kir6.1 (48 kD), Kir6.2 (44 kD) and SUR 2b (140–150 kD), but not SUR 2a (140–150 kD) and SUR 1 (175 kD), using HPAEC s and mouse brain as positive controls ( n = 3). ( C ) Confocal images showed the subcellular localization of K ATP subunits in ECFC s co‐stained with a endothelial specific marker ( CD 31 or VE ‐cadherin), revealing the extensive distribution of Kir6.1, Kir6.2 and SUR 2b. DAPI staining for nuclear labelling ( n = 3), scale bar: 20 μm. M: marker, MB : mouse brain.
    Figure Legend Snippet: The expression of K ATP subtypes in ECFC s. ( A ) RT ‐ PCR showed the expression of Kir6.1, Kir6.2 and SUR 2b, but not SUR 2a and SUR 1 in mRNA level. HPAEC s and mouse brain were used as positive controls, water (no template) as a negative control ( n = 3). ( B ) Western blotting confirmed the expression of Kir6.1 (48 kD), Kir6.2 (44 kD) and SUR 2b (140–150 kD), but not SUR 2a (140–150 kD) and SUR 1 (175 kD), using HPAEC s and mouse brain as positive controls ( n = 3). ( C ) Confocal images showed the subcellular localization of K ATP subunits in ECFC s co‐stained with a endothelial specific marker ( CD 31 or VE ‐cadherin), revealing the extensive distribution of Kir6.1, Kir6.2 and SUR 2b. DAPI staining for nuclear labelling ( n = 3), scale bar: 20 μm. M: marker, MB : mouse brain.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Negative Control, Western Blot, Staining, Marker

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    Alomone Labs kir6 2
    Relative expression of Kir6.1, <t>Kir6.2,</t> SUR2A, and SUR2B mRNA. A, Kir6.1, (B) SUR2B, (C) Kir6.2, and (D) SUR2A. Data shown are the means ± standard error of the mean of 6 separate experiments. * P
    Kir6 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kir6 2/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kir6 2 - by Bioz Stars, 2022-01
    88/100 stars
      Buy from Supplier

    90
    Alomone Labs antibodies against kir6 1
    Nico ameliorated LPS-induced ALI and inflammation. (a) Nico increased LPS-induced <t>Kir6.1</t> and Kir6.2 downregulation in the lung. (b, c) Lung sections stained with H E showed severe injury in the LPS group which was attenuated by Nico pretreatment. The data revealed a high score for the LPS-treated group which was decreased in the Nico-pretreated group. (d) Nico pretreatment significantly reduced LPS-induced protein leakage in BALF. (e, f) Nico alleviated LPS-induced increments of MPO activities in BALF and lung homogenate. (g, h) Nico prevented the production of TNF- α and IL-1 β in lung homogenate. Data were shown as mean ± SEM ( n = 6 − 8). Statistically significant differences: ∗ P
    Antibodies Against Kir6 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against kir6 1/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against kir6 1 - by Bioz Stars, 2022-01
    90/100 stars
      Buy from Supplier

    94
    Alomone Labs anti kir6 2
    Confocal double immunofluorescence images of CD11b (red, A and D) and <t>Kir6.2</t> (green, B and E) in spinal cord sections from healthy control mice (Ctrl) or MOG 35-55 EAE mice . A slight intensity was found for Kir6.2 in healthy section showing low localization of the K ATP Kir6.2 subunit in CD11b-positive cells (white arrows, C). However, higher intensity of Kir6.2 subunit in CD11b reactive cells showing a strong colocalization of both (white arrows, F) was observed. Western blotting for Kir6.2 in total protein homogenates from lumbar-sacral and thoracic-cervical regions of the spinal cord from non-immunized control animals (control, G) and EAE mice (EAE, G) show an increase in Kir6.2 expression in EAE mice. This increase is statistically significant in the thoracic-cervical level of the spinal cord (H). Results are shown as mean ± SEM. ** p
    Anti Kir6 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kir6 2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kir6 2 - by Bioz Stars, 2022-01
    94/100 stars
      Buy from Supplier

    Image Search Results


    Relative expression of Kir6.1, Kir6.2, SUR2A, and SUR2B mRNA. A, Kir6.1, (B) SUR2B, (C) Kir6.2, and (D) SUR2A. Data shown are the means ± standard error of the mean of 6 separate experiments. * P

    Journal: Journal of Cardiovascular Pharmacology

    Article Title: Altered KATP Channel Subunits Expression and Vascular Reactivity in Spontaneously Hypertensive Rats With Age

    doi: 10.1097/FJC.0000000000000394

    Figure Lengend Snippet: Relative expression of Kir6.1, Kir6.2, SUR2A, and SUR2B mRNA. A, Kir6.1, (B) SUR2B, (C) Kir6.2, and (D) SUR2A. Data shown are the means ± standard error of the mean of 6 separate experiments. * P

    Article Snippet: The primary antibody dilutions were 1:300 for Kir6.1 (ABcam, Britain), 1:500 for Kir6.2 (Alomone, Israel), 1:300 for SUR2B (Santa, Japan), and 1:30,000 for GAPDH (ABcam) antibodies.

    Techniques: Expressing

    Nico ameliorated LPS-induced ALI and inflammation. (a) Nico increased LPS-induced Kir6.1 and Kir6.2 downregulation in the lung. (b, c) Lung sections stained with H E showed severe injury in the LPS group which was attenuated by Nico pretreatment. The data revealed a high score for the LPS-treated group which was decreased in the Nico-pretreated group. (d) Nico pretreatment significantly reduced LPS-induced protein leakage in BALF. (e, f) Nico alleviated LPS-induced increments of MPO activities in BALF and lung homogenate. (g, h) Nico prevented the production of TNF- α and IL-1 β in lung homogenate. Data were shown as mean ± SEM ( n = 6 − 8). Statistically significant differences: ∗ P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Nicorandil Attenuates LPS-Induced Acute Lung Injury by Pulmonary Endothelial Cell Protection via NF-κB and MAPK Pathways

    doi: 10.1155/2019/4957646

    Figure Lengend Snippet: Nico ameliorated LPS-induced ALI and inflammation. (a) Nico increased LPS-induced Kir6.1 and Kir6.2 downregulation in the lung. (b, c) Lung sections stained with H E showed severe injury in the LPS group which was attenuated by Nico pretreatment. The data revealed a high score for the LPS-treated group which was decreased in the Nico-pretreated group. (d) Nico pretreatment significantly reduced LPS-induced protein leakage in BALF. (e, f) Nico alleviated LPS-induced increments of MPO activities in BALF and lung homogenate. (g, h) Nico prevented the production of TNF- α and IL-1 β in lung homogenate. Data were shown as mean ± SEM ( n = 6 − 8). Statistically significant differences: ∗ P

    Article Snippet: Then, the transferred membranes were incubated with primary antibodies against Kir6.1 (Alomone Labs, Jerusalem, Israel), Kir6.2 (Abcam), NF-κ B p-p65/p65, p-iκ B-α /iκ B-α , p-p38/p38, p-ERK/ERK, p-JNK/JNK, intercellular adhesion molecule-1 (ICAM-1), cleaved-caspase-3 (c-caspase-3), caspase-9 (1 : 1000, Cell Signaling Technology), endothelial nitric oxide synthase (eNOS) (1 : 1000, Santa Cruz), inducible nitric oxide synthase (iNOS) (1 : 1000, Millipore), CCAAT/enhancer-binding protein homologous protein (CHOP), vascular cell adhesion molecule-1 (VCAM-1), VE-cadherin, Nox4 (1 : 1000), MnSOD (1 : 5000, Abcam), and β -actin (1 : 5000, Proteintech, Rosemont, USA) overnight.

    Techniques: Staining

    Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Journal:

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    doi: 10.1016/j.bbrc.2007.11.154

    Figure Lengend Snippet: Detection of Kir6.1 Immunoreactivity in Mitochondrial Membranes

    Article Snippet: This experiment was particularly revealing since this Kir6.1 antibody did recognize Kir6.1 overexpressed transiently in HEK293 cells ( ).

    Techniques:

    Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Journal:

    Article Title: Is Kir6.1 a subunit of mitoKATP?

    doi: 10.1016/j.bbrc.2007.11.154

    Figure Lengend Snippet: Mitochondrial Kir6.1-Immunoreactivity Revealed by a Second Antibody

    Article Snippet: This experiment was particularly revealing since this Kir6.1 antibody did recognize Kir6.1 overexpressed transiently in HEK293 cells ( ).

    Techniques:

    Confocal double immunofluorescence images of CD11b (red, A and D) and Kir6.2 (green, B and E) in spinal cord sections from healthy control mice (Ctrl) or MOG 35-55 EAE mice . A slight intensity was found for Kir6.2 in healthy section showing low localization of the K ATP Kir6.2 subunit in CD11b-positive cells (white arrows, C). However, higher intensity of Kir6.2 subunit in CD11b reactive cells showing a strong colocalization of both (white arrows, F) was observed. Western blotting for Kir6.2 in total protein homogenates from lumbar-sacral and thoracic-cervical regions of the spinal cord from non-immunized control animals (control, G) and EAE mice (EAE, G) show an increase in Kir6.2 expression in EAE mice. This increase is statistically significant in the thoracic-cervical level of the spinal cord (H). Results are shown as mean ± SEM. ** p

    Journal: Journal of Neuroinflammation

    Article Title: Oral administration of the KATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis

    doi: 10.1186/1742-2094-8-149

    Figure Lengend Snippet: Confocal double immunofluorescence images of CD11b (red, A and D) and Kir6.2 (green, B and E) in spinal cord sections from healthy control mice (Ctrl) or MOG 35-55 EAE mice . A slight intensity was found for Kir6.2 in healthy section showing low localization of the K ATP Kir6.2 subunit in CD11b-positive cells (white arrows, C). However, higher intensity of Kir6.2 subunit in CD11b reactive cells showing a strong colocalization of both (white arrows, F) was observed. Western blotting for Kir6.2 in total protein homogenates from lumbar-sacral and thoracic-cervical regions of the spinal cord from non-immunized control animals (control, G) and EAE mice (EAE, G) show an increase in Kir6.2 expression in EAE mice. This increase is statistically significant in the thoracic-cervical level of the spinal cord (H). Results are shown as mean ± SEM. ** p

    Article Snippet: Cells were then incubated with primary antibodies anti-Kir6.1 and anti-Kir6.2 (1:300 dilution, Alomone, Jerusalem, Israel), anti-CD11b (1:500 dilution, Serotec, Oxford, England, UK) at 4°C overnight, followed by secondary antibodies Alexa®488 and 596 (1:500, Molecular Probes, Invitrogen, Eugene, OR, USA) for 1 h in blocking solution.

    Techniques: Immunofluorescence, Mouse Assay, Western Blot, Expressing

    Western blotting show expression of both Kir6.1 and Kir6.2 K ATP channel pore-forming subunits in unstimulated and LPS/IFNγ-stimulated BV-2 cells (A, left) and microglial primary cultures (A, Right). Staining for the microglial cell membrane marker CD11b (B and E) and the K ATP channel subunits Kir 6.1 (C) or Kir 6.2 (F) showed colocalization in BV-2 microglia, indicating the expression of the K ATP channel at the cytoplasmic membrane (D and G, white arrows) . Control: unstimulated cells; L+I: cells stimulated with LPS and IFNγ. Scale bar = 30 μm.

    Journal: Journal of Neuroinflammation

    Article Title: Oral administration of the KATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis

    doi: 10.1186/1742-2094-8-149

    Figure Lengend Snippet: Western blotting show expression of both Kir6.1 and Kir6.2 K ATP channel pore-forming subunits in unstimulated and LPS/IFNγ-stimulated BV-2 cells (A, left) and microglial primary cultures (A, Right). Staining for the microglial cell membrane marker CD11b (B and E) and the K ATP channel subunits Kir 6.1 (C) or Kir 6.2 (F) showed colocalization in BV-2 microglia, indicating the expression of the K ATP channel at the cytoplasmic membrane (D and G, white arrows) . Control: unstimulated cells; L+I: cells stimulated with LPS and IFNγ. Scale bar = 30 μm.

    Article Snippet: Cells were then incubated with primary antibodies anti-Kir6.1 and anti-Kir6.2 (1:300 dilution, Alomone, Jerusalem, Israel), anti-CD11b (1:500 dilution, Serotec, Oxford, England, UK) at 4°C overnight, followed by secondary antibodies Alexa®488 and 596 (1:500, Molecular Probes, Invitrogen, Eugene, OR, USA) for 1 h in blocking solution.

    Techniques: Western Blot, Expressing, Staining, Marker