Journal: PLoS ONE

Article Title: Identification of Kininogen-1 as a Serum Biomarker for the Early Detection of Advanced Colorectal Adenoma and Colorectal Cancer

doi: 10.1371/journal.pone.0070519

Figure Lengend Snippet: A: Representative mass spectra of control serum (a), serum from a patient with ACA (b), and serum from a patient with CRC (c). B: MALDI/MS/MS spectra of the ions at m/z 1943.95 (a) and m/z 2081.01 (b). Panel c provides the results from the Mascot database search, which indicated both peaks correspond to kininogen-1.

Article Snippet: Kininogen-1 concentrations were quantified using a Human Kininogen ELISA Kit (No. EK2001-1, Assaypro, MO, USA).

Techniques: Tandem Mass Spectroscopy

Journal: PLoS ONE

Article Title: Identification of Kininogen-1 as a Serum Biomarker for the Early Detection of Advanced Colorectal Adenoma and Colorectal Cancer

doi: 10.1371/journal.pone.0070519

Figure Lengend Snippet: A: The ROC curve for serum kininogen-1 (a) and CEA (b) for a diagnosis of ACA, and the ROC curve for serum kininogen-1 (c) and CEA (d) for a diagnosis of CRC. B: A comparison of the sensitivity (Se), specificity (Sp), positive predictive value (Pv+), negative predictive value (Pv-), and accuracy (Ac) rates for detection of kininogen-1 and CEA for a diagnosis of Duke’s stage A and B CRC (a) or Duke’s stage C and D CRC (b).

Article Snippet: Kininogen-1 concentrations were quantified using a Human Kininogen ELISA Kit (No. EK2001-1, Assaypro, MO, USA).

Techniques:

Journal: PLoS ONE

Article Title: Identification of Kininogen-1 as a Serum Biomarker for the Early Detection of Advanced Colorectal Adenoma and Colorectal Cancer

doi: 10.1371/journal.pone.0070519

Figure Lengend Snippet: Representative immunostainings of kininogen-1 in normal colorectal mucosa ( A ), ACA tissue ( B ), and CRC tissue ( C ). Both overview (20×) and magnification (40×) images are provided, with the latter provided as inset boxes in the top left corner of each panel. D: Quantification of kininogen-1 levels in the control, ACA, and CRC groups. E: Survival curves for control, ACA, and CRC groups. The blue line represents CRC patients negative for kininogen-1 expression and the green line represents CRC patients positive for kininogen-1 expression.

Article Snippet: Kininogen-1 concentrations were quantified using a Human Kininogen ELISA Kit (No. EK2001-1, Assaypro, MO, USA).

Techniques: Expressing

Journal: Journal of Visualized Experiments : JoVE

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

doi: 10.3791/3791

Figure Lengend Snippet:

Article Snippet: A18 , Mouse anti-human Kininogen (LMW) monoclonal antibody , Kininogen , Assaypro , 20333-05011.

Techniques: Plasmid Preparation, Positive Control