ki67 antibodies  (Boster Bio)


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    Structured Review

    Boster Bio ki67 antibodies
    Supplementation with pasteurised A. muciniphila or Amuc_1100 blunted tumourigenesis induced by azoxymethane (AOM)/DSS. (A) Weight changes are expressed as the mean change from the starting weight. (B) DAI, (C) spleen weight, (D) tumour development, number (E) and tumour area (F) were analysed. (G) Representative histological images of colon tissues by H E staining. Scale bars, 200 µm. IHC staining and quantitation of γH2AX (8 weeks, H), cleaved-caspase 3 (23 weeks, I) and <t>Ki67</t> (23 weeks, J) in the proximal colon. Scale bars, 50 µm. Data are presented as the mean±SEM and were analysed by ordinary one-way ANOVA with Tukey’s multiple comparisons. *P
    Ki67 Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ki67 antibodies/product/Boster Bio
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ki67 antibodies - by Bioz Stars, 2022-12
    94/100 stars

    Images

    1) Product Images from "A purified membrane protein from Akkermansia muciniphila or the pasteurised bacterium blunts colitis associated tumourigenesis by modulation of CD8+ T cells in mice"

    Article Title: A purified membrane protein from Akkermansia muciniphila or the pasteurised bacterium blunts colitis associated tumourigenesis by modulation of CD8+ T cells in mice

    Journal: Gut

    doi: 10.1136/gutjnl-2019-320105

    Supplementation with pasteurised A. muciniphila or Amuc_1100 blunted tumourigenesis induced by azoxymethane (AOM)/DSS. (A) Weight changes are expressed as the mean change from the starting weight. (B) DAI, (C) spleen weight, (D) tumour development, number (E) and tumour area (F) were analysed. (G) Representative histological images of colon tissues by H E staining. Scale bars, 200 µm. IHC staining and quantitation of γH2AX (8 weeks, H), cleaved-caspase 3 (23 weeks, I) and Ki67 (23 weeks, J) in the proximal colon. Scale bars, 50 µm. Data are presented as the mean±SEM and were analysed by ordinary one-way ANOVA with Tukey’s multiple comparisons. *P
    Figure Legend Snippet: Supplementation with pasteurised A. muciniphila or Amuc_1100 blunted tumourigenesis induced by azoxymethane (AOM)/DSS. (A) Weight changes are expressed as the mean change from the starting weight. (B) DAI, (C) spleen weight, (D) tumour development, number (E) and tumour area (F) were analysed. (G) Representative histological images of colon tissues by H E staining. Scale bars, 200 µm. IHC staining and quantitation of γH2AX (8 weeks, H), cleaved-caspase 3 (23 weeks, I) and Ki67 (23 weeks, J) in the proximal colon. Scale bars, 50 µm. Data are presented as the mean±SEM and were analysed by ordinary one-way ANOVA with Tukey’s multiple comparisons. *P

    Techniques Used: Staining, Immunohistochemistry, Quantitation Assay

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    Boster Bio anti ki67 antibody sp6
    MSA-2 combined with YM101 therapy improved the activities of tumor-infiltrating immune cells in EMT-6 model. a–d FACS gating strategies for tumor-infiltrating T, NK, DC, and macrophages. e–x In the FACS assays, quantification of CD3 + T, CD8 + T, activated CD8 + T (CD25 + or CD69 + ), effector memory CD8 + T (CD44 + CD62L − ), <t>Ki67</t> + CD8 + T, IFN-γ + CD8 + T, Granzyme-B + CD8 + T, NK, Ki67 + NK, IFN-γ + NK, Granzyme-B + NK, TNF-α + NK, DC, mature DC (CD80 + or CD86 + ), macrophage, M1-like macrophage (MHC-II + CD206 − ), and M2-like macrophage (MHC-II − CD206 + ). Numbers of immune cells per 100 mg EMT-6 tissue were calculated and compared. y The representative immunofluorescent staining images of tumor-infiltrating T cells. Red refers to CD4 + staining, and green refers to CD8 + staining. z Quantification of infiltration depth of T cells. * p
    Anti Ki67 Antibody Sp6, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ki67 antibody sp6/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ki67 antibody sp6 - by Bioz Stars, 2022-12
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    94
    Boster Bio rabbit anti ki 67 polyclonal antibodies
    Immunohistochemical analysis of Notch1-, <t>Ki-67-,</t> and TUNEL-positive cells in LSCC tissues. a Notch1 was observed in the cell membranes and cytoplasm of cancer cells (×400). b Ki-67 was found in the nuclei of neoplastic cells (×400). c Apoptotic cells were assessed by the TUNEL method (×400)
    Rabbit Anti Ki 67 Polyclonal Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti ki 67 polyclonal antibodies/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti ki 67 polyclonal antibodies - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    94
    Boster Bio anti ki 67 antibody
    SOAT1 is a downstream target of miR-30a-3p. A RKO and SW480 cells were transfected with miR-30a-3p mimics or inhibitors. SOAT1 expression was scouted by qRT-PCR. B Relative activities of luciferase were measured in HEK293T cells after transfection with SOAT1-Mut or SOAT1-WT and miR-30a-3p mimics or mimic NC. C Overexpression of miR-30a-3p restrained the protein production of SOAT1. Suppression of miR-30a-3p raised the expression of SOAT1 protein. D Knockdown of circLDLR (sh-circLDLR) inhibited the protein expression of SOAT1. Overexpression of circLDLR (circLDLR-OE) increased the protein expression of SOAT1. E Knockdown of miR-30a-3p reversed the sh-circLDLR-induced downregulation of SOAT1 expression in RKO cells. Overexpression of miR-30a-3p reversed the circLDLR-OE-induced upregulation of SOAT1 expression in SW480 cells. GAPDH deeded as a loading control. F EdU analysis of the <t>cell</t> <t>proliferation</t> ability in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors. G Cell migration and invasion in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were examined. H The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were assessed. I EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE. J Cell behaviors of migration and invasion in HT29 and SW480 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were examined. K The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were assessed. Data are presented as the mean ± SD of three independent experiments. * P
    Anti Ki 67 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ki 67 antibody/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ki 67 antibody - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    94
    Boster Bio antibodies against ki67
    SOAT1 is a downstream target of miR-30a-3p. A RKO and SW480 cells were transfected with miR-30a-3p mimics or inhibitors. SOAT1 expression was scouted by qRT-PCR. B Relative activities of luciferase were measured in HEK293T cells after transfection with SOAT1-Mut or SOAT1-WT and miR-30a-3p mimics or mimic NC. C Overexpression of miR-30a-3p restrained the protein production of SOAT1. Suppression of miR-30a-3p raised the expression of SOAT1 protein. D Knockdown of circLDLR (sh-circLDLR) inhibited the protein expression of SOAT1. Overexpression of circLDLR (circLDLR-OE) increased the protein expression of SOAT1. E Knockdown of miR-30a-3p reversed the sh-circLDLR-induced downregulation of SOAT1 expression in RKO cells. Overexpression of miR-30a-3p reversed the circLDLR-OE-induced upregulation of SOAT1 expression in SW480 cells. GAPDH deeded as a loading control. F EdU analysis of the <t>cell</t> <t>proliferation</t> ability in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors. G Cell migration and invasion in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were examined. H The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were assessed. I EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE. J Cell behaviors of migration and invasion in HT29 and SW480 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were examined. K The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were assessed. Data are presented as the mean ± SD of three independent experiments. * P
    Antibodies Against Ki67, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against ki67/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against ki67 - by Bioz Stars, 2022-12
    94/100 stars
      Buy from Supplier

    Image Search Results


    MSA-2 combined with YM101 therapy improved the activities of tumor-infiltrating immune cells in EMT-6 model. a–d FACS gating strategies for tumor-infiltrating T, NK, DC, and macrophages. e–x In the FACS assays, quantification of CD3 + T, CD8 + T, activated CD8 + T (CD25 + or CD69 + ), effector memory CD8 + T (CD44 + CD62L − ), Ki67 + CD8 + T, IFN-γ + CD8 + T, Granzyme-B + CD8 + T, NK, Ki67 + NK, IFN-γ + NK, Granzyme-B + NK, TNF-α + NK, DC, mature DC (CD80 + or CD86 + ), macrophage, M1-like macrophage (MHC-II + CD206 − ), and M2-like macrophage (MHC-II − CD206 + ). Numbers of immune cells per 100 mg EMT-6 tissue were calculated and compared. y The representative immunofluorescent staining images of tumor-infiltrating T cells. Red refers to CD4 + staining, and green refers to CD8 + staining. z Quantification of infiltration depth of T cells. * p

    Journal: Journal of Hematology & Oncology

    Article Title: Combination of oral STING agonist MSA-2 and anti-TGF-β/PD-L1 bispecific antibody YM101: a novel immune cocktail therapy for non-inflamed tumors

    doi: 10.1186/s13045-022-01363-8

    Figure Lengend Snippet: MSA-2 combined with YM101 therapy improved the activities of tumor-infiltrating immune cells in EMT-6 model. a–d FACS gating strategies for tumor-infiltrating T, NK, DC, and macrophages. e–x In the FACS assays, quantification of CD3 + T, CD8 + T, activated CD8 + T (CD25 + or CD69 + ), effector memory CD8 + T (CD44 + CD62L − ), Ki67 + CD8 + T, IFN-γ + CD8 + T, Granzyme-B + CD8 + T, NK, Ki67 + NK, IFN-γ + NK, Granzyme-B + NK, TNF-α + NK, DC, mature DC (CD80 + or CD86 + ), macrophage, M1-like macrophage (MHC-II + CD206 − ), and M2-like macrophage (MHC-II − CD206 + ). Numbers of immune cells per 100 mg EMT-6 tissue were calculated and compared. y The representative immunofluorescent staining images of tumor-infiltrating T cells. Red refers to CD4 + staining, and green refers to CD8 + staining. z Quantification of infiltration depth of T cells. * p

    Article Snippet: The Abs targeting PCNA (BM0104, Boster), Ki67 (ab16667, Abcam), CD8 (ab217344, Abcam), and CD4 (ab183685, Abcam) were used in the assays.

    Techniques: FACS, Staining

    MSA-2 combined with YM101 therapy altered the TME and promoted immunity-associated gene expression in B16 model. a–h Quantification of CD8 + T cell, Ki67 + CD8 + T cell, Perforin + CD8 + T cell, IFN-γ + CD8 + T cell, Granzyme-B + CD8 + T cell, CD69 + CD8 + T cell, NK cell, DC. (i) The heatmap shows the differentially expressed genes among four groups. The levels of immune-killing genes including Prf1 , Tnf , Gzma , and Gzmb were quantitatively analyzed. j–m GO enrichment analysis exploring biological processes significantly enriched in MSA-2 combined with YM101 group. n–s The scores of immune signatures. The heatmaps presenting the scaled expression level of genes constituting immune signatures. * p

    Journal: Journal of Hematology & Oncology

    Article Title: Combination of oral STING agonist MSA-2 and anti-TGF-β/PD-L1 bispecific antibody YM101: a novel immune cocktail therapy for non-inflamed tumors

    doi: 10.1186/s13045-022-01363-8

    Figure Lengend Snippet: MSA-2 combined with YM101 therapy altered the TME and promoted immunity-associated gene expression in B16 model. a–h Quantification of CD8 + T cell, Ki67 + CD8 + T cell, Perforin + CD8 + T cell, IFN-γ + CD8 + T cell, Granzyme-B + CD8 + T cell, CD69 + CD8 + T cell, NK cell, DC. (i) The heatmap shows the differentially expressed genes among four groups. The levels of immune-killing genes including Prf1 , Tnf , Gzma , and Gzmb were quantitatively analyzed. j–m GO enrichment analysis exploring biological processes significantly enriched in MSA-2 combined with YM101 group. n–s The scores of immune signatures. The heatmaps presenting the scaled expression level of genes constituting immune signatures. * p

    Article Snippet: The Abs targeting PCNA (BM0104, Boster), Ki67 (ab16667, Abcam), CD8 (ab217344, Abcam), and CD4 (ab183685, Abcam) were used in the assays.

    Techniques: Expressing

    Immunohistochemical analysis of Notch1-, Ki-67-, and TUNEL-positive cells in LSCC tissues. a Notch1 was observed in the cell membranes and cytoplasm of cancer cells (×400). b Ki-67 was found in the nuclei of neoplastic cells (×400). c Apoptotic cells were assessed by the TUNEL method (×400)

    Journal: World Journal of Surgical Oncology

    Article Title: Effect of Notch1 signaling on cellular proliferation and apoptosis in human laryngeal carcinoma

    doi: 10.1186/s12957-022-02728-6

    Figure Lengend Snippet: Immunohistochemical analysis of Notch1-, Ki-67-, and TUNEL-positive cells in LSCC tissues. a Notch1 was observed in the cell membranes and cytoplasm of cancer cells (×400). b Ki-67 was found in the nuclei of neoplastic cells (×400). c Apoptotic cells were assessed by the TUNEL method (×400)

    Article Snippet: The following primary antibodies were incubated with the tissue sections overnight at 4 °C: rabbit anti-Notch1 monoclonal antibodies (Epitomics, Inc., Burlingame, CA, USA) (1:100 dilution) and rabbit anti-Ki-67 polyclonal antibodies (BA1508, Wuhan Boster, China) (1:800 dilution).

    Techniques: Immunohistochemistry, TUNEL Assay

    SOAT1 is a downstream target of miR-30a-3p. A RKO and SW480 cells were transfected with miR-30a-3p mimics or inhibitors. SOAT1 expression was scouted by qRT-PCR. B Relative activities of luciferase were measured in HEK293T cells after transfection with SOAT1-Mut or SOAT1-WT and miR-30a-3p mimics or mimic NC. C Overexpression of miR-30a-3p restrained the protein production of SOAT1. Suppression of miR-30a-3p raised the expression of SOAT1 protein. D Knockdown of circLDLR (sh-circLDLR) inhibited the protein expression of SOAT1. Overexpression of circLDLR (circLDLR-OE) increased the protein expression of SOAT1. E Knockdown of miR-30a-3p reversed the sh-circLDLR-induced downregulation of SOAT1 expression in RKO cells. Overexpression of miR-30a-3p reversed the circLDLR-OE-induced upregulation of SOAT1 expression in SW480 cells. GAPDH deeded as a loading control. F EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors. G Cell migration and invasion in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were examined. H The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were assessed. I EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE. J Cell behaviors of migration and invasion in HT29 and SW480 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were examined. K The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were assessed. Data are presented as the mean ± SD of three independent experiments. * P

    Journal: Cell Death Discovery

    Article Title: Circular RNA circLDLR facilitates cancer progression by altering the miR-30a-3p/SOAT1 axis in colorectal cancer

    doi: 10.1038/s41420-022-01110-5

    Figure Lengend Snippet: SOAT1 is a downstream target of miR-30a-3p. A RKO and SW480 cells were transfected with miR-30a-3p mimics or inhibitors. SOAT1 expression was scouted by qRT-PCR. B Relative activities of luciferase were measured in HEK293T cells after transfection with SOAT1-Mut or SOAT1-WT and miR-30a-3p mimics or mimic NC. C Overexpression of miR-30a-3p restrained the protein production of SOAT1. Suppression of miR-30a-3p raised the expression of SOAT1 protein. D Knockdown of circLDLR (sh-circLDLR) inhibited the protein expression of SOAT1. Overexpression of circLDLR (circLDLR-OE) increased the protein expression of SOAT1. E Knockdown of miR-30a-3p reversed the sh-circLDLR-induced downregulation of SOAT1 expression in RKO cells. Overexpression of miR-30a-3p reversed the circLDLR-OE-induced upregulation of SOAT1 expression in SW480 cells. GAPDH deeded as a loading control. F EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors. G Cell migration and invasion in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were examined. H The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were assessed. I EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE. J Cell behaviors of migration and invasion in HT29 and SW480 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were examined. K The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were assessed. Data are presented as the mean ± SD of three independent experiments. * P

    Article Snippet: Sections were processed and stained with an anti-Ki-67 antibody (BOSTER, California, USA, #BM4381, 1:50), anti-CD31 antibody (Abcam, Cambridge, MA, USA, #ab32457, 1:1500) or anti-SOAT1 antibody (CST, Beverly, Ma, USA, #35695 S, 1:50).

    Techniques: Transfection, Expressing, Quantitative RT-PCR, Luciferase, Over Expression, Migration

    CircLDLR overexpression facilitates CRC malignant behaviour and increases cholesterol levels in vitro. A The expression of circLDLR in RKO and HCT116 cells transfected with a circLDLR-overexpression vector (circLDLR-OE) or the control vector (Vector) was assessed by qRT-PCR. B Cell proliferation was scouted at the indicated time points by CCK-8 assays evaluating RKO cells and HCT116 cells transfected with circLDLR-OE or Vector. C EdU analysis of the proliferative ability of RKO cells and HCT116 cells transfected with circLDLR-OE or Vector. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the EdU-positive cell percentage in transfected CRC cells is shown in the bar graph. D The cell migration and invasion of CRC cells were examined by Transwell assays after cells were transfected with circLDLR-OE or Vector. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the migrated and invaded cell numbers is shown in the bar graph. E e The production of T-CHO and LDL-C in CRC cells transfected with circLDLR-OE or Vector were appraised. Data are presented as the mean ± SD of three independent experiments. * P

    Journal: Cell Death Discovery

    Article Title: Circular RNA circLDLR facilitates cancer progression by altering the miR-30a-3p/SOAT1 axis in colorectal cancer

    doi: 10.1038/s41420-022-01110-5

    Figure Lengend Snippet: CircLDLR overexpression facilitates CRC malignant behaviour and increases cholesterol levels in vitro. A The expression of circLDLR in RKO and HCT116 cells transfected with a circLDLR-overexpression vector (circLDLR-OE) or the control vector (Vector) was assessed by qRT-PCR. B Cell proliferation was scouted at the indicated time points by CCK-8 assays evaluating RKO cells and HCT116 cells transfected with circLDLR-OE or Vector. C EdU analysis of the proliferative ability of RKO cells and HCT116 cells transfected with circLDLR-OE or Vector. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the EdU-positive cell percentage in transfected CRC cells is shown in the bar graph. D The cell migration and invasion of CRC cells were examined by Transwell assays after cells were transfected with circLDLR-OE or Vector. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the migrated and invaded cell numbers is shown in the bar graph. E e The production of T-CHO and LDL-C in CRC cells transfected with circLDLR-OE or Vector were appraised. Data are presented as the mean ± SD of three independent experiments. * P

    Article Snippet: Sections were processed and stained with an anti-Ki-67 antibody (BOSTER, California, USA, #BM4381, 1:50), anti-CD31 antibody (Abcam, Cambridge, MA, USA, #ab32457, 1:1500) or anti-SOAT1 antibody (CST, Beverly, Ma, USA, #35695 S, 1:50).

    Techniques: Over Expression, In Vitro, Expressing, Transfection, Plasmid Preparation, Quantitative RT-PCR, CCK-8 Assay, Migration

    CircLDLR suppression inhibits CRC malignant behaviour and increases cholesterol levels in vitro. A The circLDLR and mLDLR expression was assessed in RKO and HCT116 cells transfected with five independent siRNAs targeting circLDLR by qRT-PCR. B Cell proliferation was scouted at the indicated time points by CCK-8 assays evaluating HCT116 cells and RKO cells stimulated with si-NC or si-circLDLR. C EdU analysis of the proliferative ability of HCT116 cells and RKO cells treated with si-NC or si-circLDLR. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the EdU-positive cell percentage in transfected GC cells is shown in the bar graph. D The cell migration and invasion of CRC cells were examined by Transwell assays after cells were transfected with si-circLDLR or si-NC. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the migrated and invaded cell numbers is shown in the bar graph. E The production of T-CHO and LDL-C was assessed in CRC cells transfected with si-NC or si-circLDLR. Data are presented as the mean ± SD of three independent experiments. * P

    Journal: Cell Death Discovery

    Article Title: Circular RNA circLDLR facilitates cancer progression by altering the miR-30a-3p/SOAT1 axis in colorectal cancer

    doi: 10.1038/s41420-022-01110-5

    Figure Lengend Snippet: CircLDLR suppression inhibits CRC malignant behaviour and increases cholesterol levels in vitro. A The circLDLR and mLDLR expression was assessed in RKO and HCT116 cells transfected with five independent siRNAs targeting circLDLR by qRT-PCR. B Cell proliferation was scouted at the indicated time points by CCK-8 assays evaluating HCT116 cells and RKO cells stimulated with si-NC or si-circLDLR. C EdU analysis of the proliferative ability of HCT116 cells and RKO cells treated with si-NC or si-circLDLR. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the EdU-positive cell percentage in transfected GC cells is shown in the bar graph. D The cell migration and invasion of CRC cells were examined by Transwell assays after cells were transfected with si-circLDLR or si-NC. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the migrated and invaded cell numbers is shown in the bar graph. E The production of T-CHO and LDL-C was assessed in CRC cells transfected with si-NC or si-circLDLR. Data are presented as the mean ± SD of three independent experiments. * P

    Article Snippet: Sections were processed and stained with an anti-Ki-67 antibody (BOSTER, California, USA, #BM4381, 1:50), anti-CD31 antibody (Abcam, Cambridge, MA, USA, #ab32457, 1:1500) or anti-SOAT1 antibody (CST, Beverly, Ma, USA, #35695 S, 1:50).

    Techniques: In Vitro, Expressing, Transfection, Quantitative RT-PCR, CCK-8 Assay, Migration