ki67 antibodies  (Boster Bio)

Journal: Journal of Hematology & Oncology

Article Title: Combination of oral STING agonist MSA-2 and anti-TGF-β/PD-L1 bispecific antibody YM101: a novel immune cocktail therapy for non-inflamed tumors

doi: 10.1186/s13045-022-01363-8

Figure Lengend Snippet: MSA-2 combined with YM101 therapy improved the activities of tumor-infiltrating immune cells in EMT-6 model. a–d FACS gating strategies for tumor-infiltrating T, NK, DC, and macrophages. e–x In the FACS assays, quantification of CD3 + T, CD8 + T, activated CD8 + T (CD25 + or CD69 + ), effector memory CD8 + T (CD44 + CD62L − ), Ki67 + CD8 + T, IFN-γ + CD8 + T, Granzyme-B + CD8 + T, NK, Ki67 + NK, IFN-γ + NK, Granzyme-B + NK, TNF-α + NK, DC, mature DC (CD80 + or CD86 + ), macrophage, M1-like macrophage (MHC-II + CD206 − ), and M2-like macrophage (MHC-II − CD206 + ). Numbers of immune cells per 100 mg EMT-6 tissue were calculated and compared. y The representative immunofluorescent staining images of tumor-infiltrating T cells. Red refers to CD4 + staining, and green refers to CD8 + staining. z Quantification of infiltration depth of T cells. * p

Article Snippet: The Abs targeting PCNA (BM0104, Boster), Ki67 (ab16667, Abcam), CD8 (ab217344, Abcam), and CD4 (ab183685, Abcam) were used in the assays.

Techniques: FACS, Staining

Journal: Journal of Hematology & Oncology

Article Title: Combination of oral STING agonist MSA-2 and anti-TGF-β/PD-L1 bispecific antibody YM101: a novel immune cocktail therapy for non-inflamed tumors

doi: 10.1186/s13045-022-01363-8

Figure Lengend Snippet: MSA-2 combined with YM101 therapy altered the TME and promoted immunity-associated gene expression in B16 model. a–h Quantification of CD8 + T cell, Ki67 + CD8 + T cell, Perforin + CD8 + T cell, IFN-γ + CD8 + T cell, Granzyme-B + CD8 + T cell, CD69 + CD8 + T cell, NK cell, DC. (i) The heatmap shows the differentially expressed genes among four groups. The levels of immune-killing genes including Prf1 , Tnf , Gzma , and Gzmb were quantitatively analyzed. j–m GO enrichment analysis exploring biological processes significantly enriched in MSA-2 combined with YM101 group. n–s The scores of immune signatures. The heatmaps presenting the scaled expression level of genes constituting immune signatures. * p

Article Snippet: The Abs targeting PCNA (BM0104, Boster), Ki67 (ab16667, Abcam), CD8 (ab217344, Abcam), and CD4 (ab183685, Abcam) were used in the assays.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: Effect of Notch1 signaling on cellular proliferation and apoptosis in human laryngeal carcinoma

doi: 10.1186/s12957-022-02728-6

Figure Lengend Snippet: Immunohistochemical analysis of Notch1-, Ki-67-, and TUNEL-positive cells in LSCC tissues. a Notch1 was observed in the cell membranes and cytoplasm of cancer cells (×400). b Ki-67 was found in the nuclei of neoplastic cells (×400). c Apoptotic cells were assessed by the TUNEL method (×400)

Article Snippet: The following primary antibodies were incubated with the tissue sections overnight at 4 °C: rabbit anti-Notch1 monoclonal antibodies (Epitomics, Inc., Burlingame, CA, USA) (1:100 dilution) and rabbit anti-Ki-67 polyclonal antibodies (BA1508, Wuhan Boster, China) (1:800 dilution).

Techniques: Immunohistochemistry, TUNEL Assay

Journal: Cell Death Discovery

Article Title: Circular RNA circLDLR facilitates cancer progression by altering the miR-30a-3p/SOAT1 axis in colorectal cancer

doi: 10.1038/s41420-022-01110-5

Figure Lengend Snippet: SOAT1 is a downstream target of miR-30a-3p. A RKO and SW480 cells were transfected with miR-30a-3p mimics or inhibitors. SOAT1 expression was scouted by qRT-PCR. B Relative activities of luciferase were measured in HEK293T cells after transfection with SOAT1-Mut or SOAT1-WT and miR-30a-3p mimics or mimic NC. C Overexpression of miR-30a-3p restrained the protein production of SOAT1. Suppression of miR-30a-3p raised the expression of SOAT1 protein. D Knockdown of circLDLR (sh-circLDLR) inhibited the protein expression of SOAT1. Overexpression of circLDLR (circLDLR-OE) increased the protein expression of SOAT1. E Knockdown of miR-30a-3p reversed the sh-circLDLR-induced downregulation of SOAT1 expression in RKO cells. Overexpression of miR-30a-3p reversed the circLDLR-OE-induced upregulation of SOAT1 expression in SW480 cells. GAPDH deeded as a loading control. F EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors. G Cell migration and invasion in SW480 and HT29 transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were examined. H The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with miR-30a-3p inhibitors or cotransfected with si-SOAT1 and miR-30a-3p inhibitors were assessed. I EdU analysis of the cell proliferation ability in SW480 and HT29 transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE. J Cell behaviors of migration and invasion in HT29 and SW480 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were examined. K The contents of T-CHO and LDL-C in SW480 and HT29 cells transfected with circLDLR-OE or cotransfected with si-SOAT1 and circLDLR-OE were assessed. Data are presented as the mean ± SD of three independent experiments. * P

Article Snippet: Sections were processed and stained with an anti-Ki-67 antibody (BOSTER, California, USA, #BM4381, 1:50), anti-CD31 antibody (Abcam, Cambridge, MA, USA, #ab32457, 1:1500) or anti-SOAT1 antibody (CST, Beverly, Ma, USA, #35695 S, 1:50).

Techniques: Transfection, Expressing, Quantitative RT-PCR, Luciferase, Over Expression, Migration

Journal: Cell Death Discovery

Article Title: Circular RNA circLDLR facilitates cancer progression by altering the miR-30a-3p/SOAT1 axis in colorectal cancer

doi: 10.1038/s41420-022-01110-5

Figure Lengend Snippet: CircLDLR overexpression facilitates CRC malignant behaviour and increases cholesterol levels in vitro. A The expression of circLDLR in RKO and HCT116 cells transfected with a circLDLR-overexpression vector (circLDLR-OE) or the control vector (Vector) was assessed by qRT-PCR. B Cell proliferation was scouted at the indicated time points by CCK-8 assays evaluating RKO cells and HCT116 cells transfected with circLDLR-OE or Vector. C EdU analysis of the proliferative ability of RKO cells and HCT116 cells transfected with circLDLR-OE or Vector. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the EdU-positive cell percentage in transfected CRC cells is shown in the bar graph. D The cell migration and invasion of CRC cells were examined by Transwell assays after cells were transfected with circLDLR-OE or Vector. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the migrated and invaded cell numbers is shown in the bar graph. E e The production of T-CHO and LDL-C in CRC cells transfected with circLDLR-OE or Vector were appraised. Data are presented as the mean ± SD of three independent experiments. * P

Article Snippet: Sections were processed and stained with an anti-Ki-67 antibody (BOSTER, California, USA, #BM4381, 1:50), anti-CD31 antibody (Abcam, Cambridge, MA, USA, #ab32457, 1:1500) or anti-SOAT1 antibody (CST, Beverly, Ma, USA, #35695 S, 1:50).

Techniques: Over Expression, In Vitro, Expressing, Transfection, Plasmid Preparation, Quantitative RT-PCR, CCK-8 Assay, Migration

Journal: Cell Death Discovery

Article Title: Circular RNA circLDLR facilitates cancer progression by altering the miR-30a-3p/SOAT1 axis in colorectal cancer

doi: 10.1038/s41420-022-01110-5

Figure Lengend Snippet: CircLDLR suppression inhibits CRC malignant behaviour and increases cholesterol levels in vitro. A The circLDLR and mLDLR expression was assessed in RKO and HCT116 cells transfected with five independent siRNAs targeting circLDLR by qRT-PCR. B Cell proliferation was scouted at the indicated time points by CCK-8 assays evaluating HCT116 cells and RKO cells stimulated with si-NC or si-circLDLR. C EdU analysis of the proliferative ability of HCT116 cells and RKO cells treated with si-NC or si-circLDLR. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the EdU-positive cell percentage in transfected GC cells is shown in the bar graph. D The cell migration and invasion of CRC cells were examined by Transwell assays after cells were transfected with si-circLDLR or si-NC. Representative images are shown. Scale bar, 200 μm. Statistical analysis of the migrated and invaded cell numbers is shown in the bar graph. E The production of T-CHO and LDL-C was assessed in CRC cells transfected with si-NC or si-circLDLR. Data are presented as the mean ± SD of three independent experiments. * P

Article Snippet: Sections were processed and stained with an anti-Ki-67 antibody (BOSTER, California, USA, #BM4381, 1:50), anti-CD31 antibody (Abcam, Cambridge, MA, USA, #ab32457, 1:1500) or anti-SOAT1 antibody (CST, Beverly, Ma, USA, #35695 S, 1:50).

Techniques: In Vitro, Expressing, Transfection, Quantitative RT-PCR, CCK-8 Assay, Migration