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(A-F) FoG values of clinical isolates calculated as described in under the following conditions (A) YPD media and different azole drugs, FCZ=fluconazole, <t>KCZ=ketoconazole,</t> VCZ= voriconazole (B) Fluconazole and media variations, (C) Casitone minimal media and different drugs, (D) Sabouraud Dextrose Agar (SGA) and different drugs, (E) Ketoconazole, casitone media and varying temperatures, (F) Voriconazole, casitone media and varying temperature. Error bars represent the standard deviation between three separate biological replicates. The statistical tests performed are determined by a two-way Annova and the p-value ranging between (<0.0001-0.005) with a significance level ranging from highest (****) to lowest (**). (G) All FoG 20 values (in panels A-F) for a given isolate was plotted to visualise the intra-strain variability. This illustrates the impact of environmental variables on tolerance levels for each of the strains.
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sample code spee sche controltested microorganisms fungi ketoconazole aspergillus fumigatus  (ATCC)
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(A-F) FoG values of clinical isolates calculated as described in under the following conditions (A) YPD media and different azole drugs, FCZ=fluconazole, <t>KCZ=ketoconazole,</t> VCZ= voriconazole (B) Fluconazole and media variations, (C) Casitone minimal media and different drugs, (D) Sabouraud Dextrose Agar (SGA) and different drugs, (E) Ketoconazole, casitone media and varying temperatures, (F) Voriconazole, casitone media and varying temperature. Error bars represent the standard deviation between three separate biological replicates. The statistical tests performed are determined by a two-way Annova and the p-value ranging between (<0.0001-0.005) with a significance level ranging from highest (****) to lowest (**). (G) All FoG 20 values (in panels A-F) for a given isolate was plotted to visualise the intra-strain variability. This illustrates the impact of environmental variables on tolerance levels for each of the strains.
Sample Code Spee Sche Controltested Microorganisms Fungi Ketoconazole Aspergillus Fumigatus, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A-F) FoG values of clinical isolates calculated as described in under the following conditions (A) YPD media and different azole drugs, FCZ=fluconazole, KCZ=ketoconazole, VCZ= voriconazole (B) Fluconazole and media variations, (C) Casitone minimal media and different drugs, (D) Sabouraud Dextrose Agar (SGA) and different drugs, (E) Ketoconazole, casitone media and varying temperatures, (F) Voriconazole, casitone media and varying temperature. Error bars represent the standard deviation between three separate biological replicates. The statistical tests performed are determined by a two-way Annova and the p-value ranging between (<0.0001-0.005) with a significance level ranging from highest (****) to lowest (**). (G) All FoG 20 values (in panels A-F) for a given isolate was plotted to visualise the intra-strain variability. This illustrates the impact of environmental variables on tolerance levels for each of the strains.

Journal: bioRxiv

Article Title: Combined genetic and environmental factors shape the landscape of anti-fungal tolerance in Nakaseomyces glabratus

doi: 10.1101/2025.07.02.662741

Figure Lengend Snippet: (A-F) FoG values of clinical isolates calculated as described in under the following conditions (A) YPD media and different azole drugs, FCZ=fluconazole, KCZ=ketoconazole, VCZ= voriconazole (B) Fluconazole and media variations, (C) Casitone minimal media and different drugs, (D) Sabouraud Dextrose Agar (SGA) and different drugs, (E) Ketoconazole, casitone media and varying temperatures, (F) Voriconazole, casitone media and varying temperature. Error bars represent the standard deviation between three separate biological replicates. The statistical tests performed are determined by a two-way Annova and the p-value ranging between (<0.0001-0.005) with a significance level ranging from highest (****) to lowest (**). (G) All FoG 20 values (in panels A-F) for a given isolate was plotted to visualise the intra-strain variability. This illustrates the impact of environmental variables on tolerance levels for each of the strains.

Article Snippet: For the broth-microdilution assay, the drugs Fluconazole (Biorbyt #orb1223822), Voriconazole (Biorbyt #orb134756) and Ketoconazole (Biorbyt #orb545799) purchased in powder form were utilized.

Techniques: Standard Deviation

Data are plotted as doubling time (hours) against FoG 20 values for the 4 clinical isolates, MLY040, MLT063, MLT081, MLT031, and WT. Doubling times were calculated in the absence of drug at the indicated temperatures, 37 °C, 25 °C and 42 °C. FoG 20 values obtained using DDA as in with (A-C) Voriconazole (1µg) treatment or (D-F) Ketoconazole (50 µg) treatment. Data represent mean of biological triplicates.

Journal: bioRxiv

Article Title: Combined genetic and environmental factors shape the landscape of anti-fungal tolerance in Nakaseomyces glabratus

doi: 10.1101/2025.07.02.662741

Figure Lengend Snippet: Data are plotted as doubling time (hours) against FoG 20 values for the 4 clinical isolates, MLY040, MLT063, MLT081, MLT031, and WT. Doubling times were calculated in the absence of drug at the indicated temperatures, 37 °C, 25 °C and 42 °C. FoG 20 values obtained using DDA as in with (A-C) Voriconazole (1µg) treatment or (D-F) Ketoconazole (50 µg) treatment. Data represent mean of biological triplicates.

Article Snippet: For the broth-microdilution assay, the drugs Fluconazole (Biorbyt #orb1223822), Voriconazole (Biorbyt #orb134756) and Ketoconazole (Biorbyt #orb545799) purchased in powder form were utilized.

Techniques: