keratin type ii cytoskeletal 6a  (Proteintech)

Journal: Neural Regeneration Research

Article Title: Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementia

doi: 10.4103/1673-5374.211196

Figure Lengend Snippet: Two-dimensional gel electrophoresis profiles of mitochondrial precursor protein and KRT6A expression in rat models of vascular dementia with or without GC treatment Panels show enlarged regions of the 2DE protein profile. Areas outlined in green show differently expressed protein points. Mitochondrial precursor protein expression was 2.54-fold greater in the GC-treated group than in the model group. KRT6A expression was 1.58-fold lower than in the model group. Proteomic studies were conducted in triplicate. MP: Mitochondrial precursor protein; KRT6A: keratin type II cytoskeletal 6A.

Article Snippet: The membranes were incubated overnight at 4°C with rabbit anti-mitochondrial precursor protein polyclonal antibody (1:2,000; Proteintech Inc., Chicago, IL, USA) and rabbit anti-keratin type II cytoskeletal 6A (KRT6A) polyclonal antibody (1:1,000; Proteintech Inc.) in 10% non-fat milk.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Expressing

Journal: Neural Regeneration Research

Article Title: Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementia

doi: 10.4103/1673-5374.211196

Figure Lengend Snippet: Peptide mass fingerprinting. After destained and dehydrated, peptides were extracted and concentrated using C-18 Zip-Tips. Samples were prepared using a prespotted AnchorChip (PAC96) target. Mass spectrum peptide information was obtained using a 4700 MALDI TOF/TOF mass spectrometer. Peptide mass fingerprinting of (A) mitochondrial precursor protein; (B) KRT6A (type II cytoskeletal 6A) were listed above.

Article Snippet: The membranes were incubated overnight at 4°C with rabbit anti-mitochondrial precursor protein polyclonal antibody (1:2,000; Proteintech Inc., Chicago, IL, USA) and rabbit anti-keratin type II cytoskeletal 6A (KRT6A) polyclonal antibody (1:1,000; Proteintech Inc.) in 10% non-fat milk.

Techniques: Peptide Mass Fingerprinting, Mass Spectrometry

Journal: Neural Regeneration Research

Article Title: Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementia

doi: 10.4103/1673-5374.211196

Figure Lengend Snippet: Protein information of mitochondrial precursor protein and type II cytoskeletal 6A identified by time-of-flight mass spectrometry

Article Snippet: The membranes were incubated overnight at 4°C with rabbit anti-mitochondrial precursor protein polyclonal antibody (1:2,000; Proteintech Inc., Chicago, IL, USA) and rabbit anti-keratin type II cytoskeletal 6A (KRT6A) polyclonal antibody (1:1,000; Proteintech Inc.) in 10% non-fat milk.

Techniques: Mass Spectrometry

Journal: Neural Regeneration Research

Article Title: Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementia

doi: 10.4103/1673-5374.211196

Figure Lengend Snippet: Western blot assay of mitochondrial precursor protein and KRT6A in the hippocampus of rat models of vascular dementia. Data are expressed as the mean ± SD. * P < 0.05, vs . control group; # P < 0.05, vs . VD group (two-way analysis of variance). Western blot assays were conducted in triplicate. GC: Glycosides of Cistanche ; VD: vascular dementia; MP: mitochondrial precursor protein; KRT6A: keratin type II cytoskeletal 6A.

Article Snippet: The membranes were incubated overnight at 4°C with rabbit anti-mitochondrial precursor protein polyclonal antibody (1:2,000; Proteintech Inc., Chicago, IL, USA) and rabbit anti-keratin type II cytoskeletal 6A (KRT6A) polyclonal antibody (1:1,000; Proteintech Inc.) in 10% non-fat milk.

Techniques: Western Blot