Journal: Food Science & Nutrition

Article Title: Gastrodia elata Polysaccharide Attenuates Aging by Modulating Oxidative Stress, Inflammation, and Gut Microbiota

doi: 10.1002/fsn3.72413

Figure Lengend Snippet: GEP reverses oxidative stress in aging mice via the Nrf2/Keap1 antioxidant signaling pathway. (A–C) Levels of MDA (A), SOD activity (B), and CAT activity (C) in mouse serum. (D–F) Levels of MDA (D), SOD activity (E), and CAT activity (F) in mouse brain tissues. (G, H) Representative protein bands of the Nrf2/Keap1 antioxidant signaling pathway in mouse brain tissues (G) and their quantitative analysis (H–K). All experimental data are presented as mean ± SD. n = 6, ns > 0.05, #### p < 0.0001 versus Control group; ** p < 0.01, *** p < 0.001, and **** p < 0.0001 versus Aging group.

Article Snippet: Membranes were incubated with primary antibodies against Nrf2 (1:400, sc‐365949, Santa Cruz), Keap1 (1:800, A25951 , ABclonal), HO‐1 (1:500, ab13248, Abcam), NQO1 (1:5000, A19586, ABclonal), TLR4 (1:600, Sc‐293072; Santa Cruz), P65 (1:600, Sc‐8008; Santa Cruz), phospho‐P65 (1:600, Sc‐136548, Santa Cruz), and GAPDH (1:10,000, 60004‐1‐Ig, Proteintech).

Techniques: Activity Assay, Analysis, Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Effects of Dietary DL-Methionine Supplementation on Meat Quality and Antioxidant Capacity in Wenchang Chickens Subjected to Chronic Heat Stress

doi: 10.3390/ani16142164

Figure Lengend Snippet: Effect of DL-methionine supplementation on relative mRNA expression levels of antioxidant-related genes in breast muscle of Wenchang chickens under heat stress conditions. ( A ) Relative expression level of Keap1 mRNA in the four groups; ( B ) relative expression level of Nrf2 mRNA in the four groups; ( C ) relative expression level of GSH-Px mRNA in the four groups; ( D ) relative expression level of CAT mRNA in the four groups; ( E ) relative expression level of SOD mRNA in the four groups. Note: Differences marked with different lowercase letters indicate significant statistical differences ( p < 0.05), and data are presented as means ± SEM ( n = 6).

Article Snippet: KEAP1 Rabbit pAb , 1:500 , ABclonal (Woburn, MA, USA) , A1820.

Techniques: Expressing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Effects of Dietary DL-Methionine Supplementation on Meat Quality and Antioxidant Capacity in Wenchang Chickens Subjected to Chronic Heat Stress

doi: 10.3390/ani16142164

Figure Lengend Snippet: Effect of DL-methionine supplementation on relative mRNA expression levels of antioxidant-related genes in thigh muscles of Wenchang chickens under heat stress conditions. ( A ) Relative expression level of Keap1 mRNA in the four groups; ( B ) relative expression level of Nrf2 mRNA in the four groups; ( C ) relative expression level of GSH-Px mRNA in the four groups; ( D ) relative expression level of CAT mRNA in the four groups; ( E ) relative expression level of SOD mRNA in the four groups. Note: Differences marked with different lowercase letters indicate significant statistical differences ( p < 0.05), and data are presented as means ± SEM ( n = 6).

Article Snippet: KEAP1 Rabbit pAb , 1:500 , ABclonal (Woburn, MA, USA) , A1820.

Techniques: Expressing, Muscles

Journal: Animals : an Open Access Journal from MDPI

Article Title: Effects of Dietary DL-Methionine Supplementation on Meat Quality and Antioxidant Capacity in Wenchang Chickens Subjected to Chronic Heat Stress

doi: 10.3390/ani16142164

Figure Lengend Snippet: Effects of DL-methionine supplementation on Keap1 and Nrf2 protein expression levels in breast muscle of Wenchang chickens under heat stress conditions. ( A ) Representative Western blot images of Keap1 protein in breast muscle samples from Group 1 (control, 0.00%) and 0.20% (Group 3). β-Actin was used as a loading control. ( B ) Quantitative analysis of Keap1 protein expression normalized to β-actin. Data are presented as means ± SD. p < 0.05 vs. Group 1. ( C ) Representative Western blot images of Nrf2 protein in breast muscle samples from Group 1 (control, 0.00%) and 0.20% (Group 3). β-Actin was used as a loading control. ( D ) Quantitative analysis of Nrf2 protein expression normalized to β-actin. Data are presented as means ± SD. p < 0.05 vs. Group 1. Note: * Asterisks in the figure indicate significant differences compared with Group 1 (control group). *: p < 0.05.

Article Snippet: KEAP1 Rabbit pAb , 1:500 , ABclonal (Woburn, MA, USA) , A1820.

Techniques: Expressing, Western Blot, Control