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A , gene expression of fission and fusion markers Fis1, Drp1, <t>OPA1</t> and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
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A , gene expression of fission and fusion markers Fis1, Drp1, <t>OPA1</t> and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
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A , gene expression of fission and fusion markers Fis1, Drp1, <t>OPA1</t> and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
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keap 1  (Proteintech)
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A , gene expression of fission and fusion markers Fis1, Drp1, <t>OPA1</t> and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.
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keap 1  (ABclonal Biotechnology)
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A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).
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keap1  (ABclonal Biotechnology)
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ABclonal Biotechnology keap1
A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).
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keap1  (Santa Cruz Biotechnology)
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A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).
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Image Search Results


A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

Journal: The Journal of Physiology

Article Title: Mitochondrial dynamics and repair differences in two mouse models of Friedreich's ataxia

doi: 10.1113/JP291356

Figure Lengend Snippet: A , gene expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD hearts normalized to controls. B , western blot images of the corresponding proteins involved in mitochondrial fission–fusion processes. C , protein expression of fission and fusion markers Fis1, Drp1, OPA1 and MFN2 in FXN‐cKO and FXNKD mice normalized to controls. D , protein expression of OPA1‐l/OPA1‐s ratio in FXN‐cKO hearts. E , OPA1‐l/OPA1‐s ratio in FXNKD hearts. F , schematic representation of fusion and fission. N = 8 mouse hearts per genotype for gene expression and n = 4 mouse hearts per genotype for protein expression. Numbers above the brackets indicate the exact P ‐value.

Article Snippet: Primary antibodies: TFAM (Cell Signalling Technology, 8076), DRP1 (Cell Signalling Technology, 8570), FIS1 (Cell Signalling Technology, 32525), MFN2 (Abcam, ab56889), OPA1 (Cell Signalling Technology, 8047), Pink1 (Abcam, 23707), Parkin (Cell Signalling Technology, 32833), p62 (Abcam, ab56416), FUNDC1 (Cell Signalling Technology, 49240), BNIP3L (Cell Signalling Technology, 12396), LC3 (Cell Signalling Technology, 12 741), ATG7 (Cell Signalling Technology, 8558), ATG12–5 (Cell Signalling Technology, 4180), LAMP1 (Abcam, 24170), CD63 (Cell Signalling Technology, 10112), Cathepsin D (Cell Signalling, 74089), GAPDH (Abcam, ab8245), αTubulin (Millipore Sigma, T6199).

Techniques: Gene Expression, Western Blot, Expressing

A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).

Journal: bioRxiv

Article Title: Combined celecoxib and NDGA induces a non-canonical, autophagy-independent redox cell death via p62/KEAP1 in colorectal cancer

doi: 10.64898/2026.09.29.753356

Figure Lengend Snippet: A Western blot analysis of total p62, phospho-p62 (S349), KEAP1, NRF2, and phospho-ULK1 in HCT116 and HT29 cells after 24 hours of treatment with the mentioned compounds. GAPDH served as a loading control. B-D Densitometric quantification of phospho-p62/total p62 ratio, KEAP1, and NRF2 in HCT116 and HT29 cells. Values were normalized to GAPDH. Data are shown as mean ± SD (n=3). * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001 versus single agents (celecoxib or NDGA alone).

Article Snippet: NRF-2 (A3577), KEAP-1 (A25951), SQSTM1/p62 (A19700), Phospho-SQSTM1/p62-S349 (A20737), and ALOX5 (A2877) phosphor-ULK1 S555 (AP1495) were purchased from ABclonal (Woburn, MA, USA).

Techniques: Western Blot, Analysis, Control