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kchip2  (Alomone Labs)


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    Structured Review

    Alomone Labs kchip2
    Localization and expression of potassium channel-associated proteins in mouse atrial myocytes. A. Immunofluorescence images showing membrane localization and expression of Kv4.3, Kv4.2, <t>KChIP2,</t> Kv1.5, and Kir2.1 in atrial cardiomyocytes from LFD and HFD groups. Scale bar, 100 μm. B. Representative Western blotting images and densitometric quantification of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 protein expression in atrial cardiomyocytes from LFD and HFD mice. LFD, low-fat diet; HFD, high-fat diet. Data are presented as mean ± SD ( n = 3). ns, not significant; * p < 0.05, ** p < 0.01 vs. LFD group.
    Kchip2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kchip2/Anti-KChIP2+Antibody/pmc12773632-188-39-41
    Average 94 stars, based on 11 article reviews
    kchip2 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice"

    Article Title: Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice

    Journal: Channels

    doi: 10.1080/19336950.2025.2611704

    Localization and expression of potassium channel-associated proteins in mouse atrial myocytes. A. Immunofluorescence images showing membrane localization and expression of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 in atrial cardiomyocytes from LFD and HFD groups. Scale bar, 100 μm. B. Representative Western blotting images and densitometric quantification of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 protein expression in atrial cardiomyocytes from LFD and HFD mice. LFD, low-fat diet; HFD, high-fat diet. Data are presented as mean ± SD ( n = 3). ns, not significant; * p < 0.05, ** p < 0.01 vs. LFD group.
    Figure Legend Snippet: Localization and expression of potassium channel-associated proteins in mouse atrial myocytes. A. Immunofluorescence images showing membrane localization and expression of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 in atrial cardiomyocytes from LFD and HFD groups. Scale bar, 100 μm. B. Representative Western blotting images and densitometric quantification of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 protein expression in atrial cardiomyocytes from LFD and HFD mice. LFD, low-fat diet; HFD, high-fat diet. Data are presented as mean ± SD ( n = 3). ns, not significant; * p < 0.05, ** p < 0.01 vs. LFD group.

    Techniques Used: Expressing, Immunofluorescence, Membrane, Western Blot

    Related Articles

    Incubation:

    Article Title: Glucagon-Like Peptide-1 Protects Against Atrial Fibrillation and Atrial Remodeling in Type 2 Diabetic Mice
    Article Snippet: The membrane was blocked for 1 hour at room temperature with 1% casein in Tris-buffered saline (TBS; Bio-Rad Laboratories). .. Blots were incubated overnight at 4 °C with rabbit-raised primary antibodies to Kv4.2 (1:500, Alomone Labs), Kv4.3 (1:1,000, Alomone Labs), Kv1.5 (1:500, Alomone Labs) or KChIP2 (1:500, Alomone Labs). .. The Western blot membranes were then washed 3 times for 10 min in 1% TBST (TBS with 1% Tween 20 (Bio-Rad Laboratories)) before secondary antibody binding.

    Western Blot:

    Article Title: Intravenous Semaphorin 3A Administration Maintains Cardiac Contractility and Improves Electrical Remodeling in a Mouse Model of Isoproterenol-Induced Heart Failure.
    Article Snippet: .. The samples were subjected to Western blotting with antibodies against Kv4.3 (#APC-017; Alomone Labs, Inc., Jerusalem, Israel), KChIP2 (#APC-142; Alomone Labs), sarcoendoplasmic reticulum Ca2+-ATPase 2 (SERCA2; #ab3625; Abcam, Cambridge, UK), and GAPDH (#GTX28245; GeneTexR, Inc., Irvine, CA, USA). .. Rabbit IgG HRP-conjugated Whole Ab (#Cytiva NA934; Sigma) was used as the secondary antibody.

    Blocking Assay:

    Article Title: Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice.
    Article Snippet: After transfer, membranes were blocked with 5% skim milk (Solarbio, China) in TBST (Solarbio, China) for 2 h at room temperature. .. Primary antibodies diluted in blocking buffer were applied overnight at 4°C as follows: resistin (1:1,000; Abcam, USA), chemerin (1:1,000; Abcam, USA), leptin (1:1,000; Abcam, USA), CMKLR1 (1:1,000; Invitrogen, USA), Kv4.3 (1:1,000; Alomone Labs, Israel), Kv4.2 (1:1,000; Alomone Labs, Israel), KChIP2 (1:1,000; Alomone Labs, Israel), Kv1.5 (1:1,000; Alomone Labs, Israel), Kir2.1 (1:1,000; Alomone Labs, Israel), β-Tubulin (1:1,000; Abcam, USA). .. Membranes were washed three times with TBST (10 min each) and then incubated for1 h at room temperature with HRP-conjugated goat anti-rabbit IgG (1:10,000; Solarbio, China) for detection of rabbit primary antibodies.

    Article Title: Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice
    Article Snippet: After transfer, membranes were blocked with 5% skim milk (Solarbio, China) in TBST (Solarbio, China) for 2 h at room temperature. .. Primary antibodies diluted in blocking buffer were applied overnight at 4°C as follows: resistin (1:1,000; Abcam, USA), chemerin (1:1,000; Abcam, USA), leptin (1:1,000; Abcam, USA), CMKLR1 (1:1,000; Invitrogen, USA), Kv4.3 (1:1,000; Alomone Labs, Israel), Kv4.2 (1:1,000; Alomone Labs, Israel), KChIP2 (1:1,000; Alomone Labs, Israel), Kv1.5 (1:1,000; Alomone Labs, Israel), Kir2.1 (1:1,000; Alomone Labs, Israel), β-Tubulin (1:1,000; Abcam, USA). .. Membranes were washed three times with TBST (10 min each) and then incubated for1 h at room temperature with HRP-conjugated goat anti-rabbit IgG (1:10,000; Solarbio, China) for detection of rabbit primary antibodies.

    Control:

    Article Title: Knockout of interleukin-17A diminishes ventricular arrhythmia susceptibility in diabetic mice via inhibiting NF-κB-mediated electrical remodeling.
    Article Snippet: Interleukin-17A (IL-17), a potent proinflammatory cytokine, has been shown to participate in cardiac electrical disorders.. Diabetes mellitus is an independent risk factor for ventricular arrhythmia.. In this study, we investigated the role of IL-17 in ventricular arrhythmia of diabetic mice.



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    Image Search Results


    Localization and expression of potassium channel-associated proteins in mouse atrial myocytes. A. Immunofluorescence images showing membrane localization and expression of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 in atrial cardiomyocytes from LFD and HFD groups. Scale bar, 100 μm. B. Representative Western blotting images and densitometric quantification of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 protein expression in atrial cardiomyocytes from LFD and HFD mice. LFD, low-fat diet; HFD, high-fat diet. Data are presented as mean ± SD ( n = 3). ns, not significant; * p < 0.05, ** p < 0.01 vs. LFD group.

    Journal: Channels

    Article Title: Association of the chemerin-CMKLR1 with atrial potassium current dysregulation and atrial fibrillation in obese mice

    doi: 10.1080/19336950.2025.2611704

    Figure Lengend Snippet: Localization and expression of potassium channel-associated proteins in mouse atrial myocytes. A. Immunofluorescence images showing membrane localization and expression of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 in atrial cardiomyocytes from LFD and HFD groups. Scale bar, 100 μm. B. Representative Western blotting images and densitometric quantification of Kv4.3, Kv4.2, KChIP2, Kv1.5, and Kir2.1 protein expression in atrial cardiomyocytes from LFD and HFD mice. LFD, low-fat diet; HFD, high-fat diet. Data are presented as mean ± SD ( n = 3). ns, not significant; * p < 0.05, ** p < 0.01 vs. LFD group.

    Article Snippet: Primary antibodies diluted in blocking buffer were applied overnight at 4°C as follows: resistin (1:1,000; Abcam, USA), chemerin (1:1,000; Abcam, USA), leptin (1:1,000; Abcam, USA), CMKLR1 (1:1,000; InvitrogenTM, USA), Kv4.3 (1:1,000; Alomone Labs, Israel), Kv4.2 (1:1,000; Alomone Labs, Israel), KChIP2 (1:1,000; Alomone Labs, Israel), Kv1.5 (1:1,000; Alomone Labs, Israel), Kir2.1 (1:1,000; Alomone Labs, Israel), β-Tubulin (1:1,000; Abcam, USA).

    Techniques: Expressing, Immunofluorescence, Membrane, Western Blot

    Effects of NS5806 on I to channel-related gene and protein expression. ( A – C ) Quantification of the mRNA levels of Kv4.2 ( A ), KChIP2 ( B ) and DPP6 ( C ). GAPDH served as an internal control (n = 9 in each group). ( D ) Representative immunoblots. Original blots are presented in Supplementary Figure . ( E – G ) Summary data of Kv4.2 ( E ), KChIP2 ( F ) and DPP6 ( G ) protein levels. GAPDH served as an internal control (n = 5 in each group). * P < 0.05, ** P < 0.01 versus the Sham + Vehicle group. # P < 0.05, ## P < 0.01 versus TAC + Vehicle.

    Journal: Scientific Reports

    Article Title: The Kv4 potassium channel modulator NS5806 attenuates cardiac hypertrophy in vivo and in vitro

    doi: 10.1038/s41598-024-70962-x

    Figure Lengend Snippet: Effects of NS5806 on I to channel-related gene and protein expression. ( A – C ) Quantification of the mRNA levels of Kv4.2 ( A ), KChIP2 ( B ) and DPP6 ( C ). GAPDH served as an internal control (n = 9 in each group). ( D ) Representative immunoblots. Original blots are presented in Supplementary Figure . ( E – G ) Summary data of Kv4.2 ( E ), KChIP2 ( F ) and DPP6 ( G ) protein levels. GAPDH served as an internal control (n = 5 in each group). * P < 0.05, ** P < 0.01 versus the Sham + Vehicle group. # P < 0.05, ## P < 0.01 versus TAC + Vehicle.

    Article Snippet: Primary antibodies against Kv4.2 (1:500, 75–016, NeuroMab, USA), KChIP2 (1:500, DF14620, Affinity, China), DPP6 (1:200, APC-146, Alomone, Israel) and GAPDH (1:10,000, 10494-1-AP, Proteintech, China) were incubated at 4 °C overnight.

    Techniques: Expressing, Control, Western Blot

    Fig. 6. DAPA alleviated ion channel disorders and atrial fibrosis caused by Ang II. A. Immunoblotting analysis of the protein expression of CAMKII, ox-CAMKII, Nav1.5, Kv4.3, Kv4.2, Kchip2, Kir2.1, Cx40, smad2/3, p-smad2/3, TGF-β1 in atrial tissue. B, C. Quantification of CAMKII, ox-CAMKII expression level in atrial tissue. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. D-I. Quantification of atrial electrical activity associated protein expression level. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. J, K. Quantification of atrial fibrosis associated protein expression level. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. Statistical significance was assessed by one-way ANOVA analysis followed by Tukey tests or Kruskal-Wallis post hoc multicomparison test (B–K). Data are presented as means ± SEM.

    Journal: European journal of pharmacology

    Article Title: Dapagliflozin: A sodium-glucose cotransporter 2 inhibitor, attenuates angiotensin II-induced atrial fibrillation by regulating atrial electrical and structural remodeling.

    doi: 10.1016/j.ejphar.2024.176712

    Figure Lengend Snippet: Fig. 6. DAPA alleviated ion channel disorders and atrial fibrosis caused by Ang II. A. Immunoblotting analysis of the protein expression of CAMKII, ox-CAMKII, Nav1.5, Kv4.3, Kv4.2, Kchip2, Kir2.1, Cx40, smad2/3, p-smad2/3, TGF-β1 in atrial tissue. B, C. Quantification of CAMKII, ox-CAMKII expression level in atrial tissue. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. D-I. Quantification of atrial electrical activity associated protein expression level. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. J, K. Quantification of atrial fibrosis associated protein expression level. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. Statistical significance was assessed by one-way ANOVA analysis followed by Tukey tests or Kruskal-Wallis post hoc multicomparison test (B–K). Data are presented as means ± SEM.

    Article Snippet: Protein lysates of tissue or cells were separated by 8% SDS-PAGE and transferred to NC membrane, and blocked for 2 h using 5% non-fat milk and probed with primary polyclonal antibodies against CaMKII (Abclonal), oxCaMKII (GeneTex), Nav1.5 (Abmart), Kv4.3 (Abclonal), Kv4.2 (Cell Signaling Technology), Kchip2 (Abclonal), Kir2.1(Zenbio), Cx40 (GeneTex), TGF-β1(Santa cruz), smad (Zenbio), p-smad (Zenbio) at 4 ◦C overnight.

    Techniques: Western Blot, Expressing, Activity Assay

    Fig. 7. SGLT2i alleviated ion channel disorders and atrial fibrosis caused by activation of CAMKII. A-I Immunoblotting analysis and quantitation of the protein expression of CAMKII, ox-CAMKII, Nav1.5, Kv4.3, Kv4.2, Kchip2, Kir2.1, Cx40 in HL-1 atrial myocytes. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. J-N. Immunoblotting analysis and quantitation of the protein expression of CAMKII, ox-CAMKII, smad2/3, p-smad2/3, TGF-β1 in primary mouse fibroblast. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. Statistical significance was assessed by one-way ANOVA analysis followed by Tukey tests or Kruskal- Wallis post hoc multicomparison test (B–I and K–N). Data are presented as means ± SEM.

    Journal: European journal of pharmacology

    Article Title: Dapagliflozin: A sodium-glucose cotransporter 2 inhibitor, attenuates angiotensin II-induced atrial fibrillation by regulating atrial electrical and structural remodeling.

    doi: 10.1016/j.ejphar.2024.176712

    Figure Lengend Snippet: Fig. 7. SGLT2i alleviated ion channel disorders and atrial fibrosis caused by activation of CAMKII. A-I Immunoblotting analysis and quantitation of the protein expression of CAMKII, ox-CAMKII, Nav1.5, Kv4.3, Kv4.2, Kchip2, Kir2.1, Cx40 in HL-1 atrial myocytes. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. J-N. Immunoblotting analysis and quantitation of the protein expression of CAMKII, ox-CAMKII, smad2/3, p-smad2/3, TGF-β1 in primary mouse fibroblast. n = 4. *p < 0.05 vs. CTL group and #p < 0.05 vs. Ang II group. Statistical significance was assessed by one-way ANOVA analysis followed by Tukey tests or Kruskal- Wallis post hoc multicomparison test (B–I and K–N). Data are presented as means ± SEM.

    Article Snippet: Protein lysates of tissue or cells were separated by 8% SDS-PAGE and transferred to NC membrane, and blocked for 2 h using 5% non-fat milk and probed with primary polyclonal antibodies against CaMKII (Abclonal), oxCaMKII (GeneTex), Nav1.5 (Abmart), Kv4.3 (Abclonal), Kv4.2 (Cell Signaling Technology), Kchip2 (Abclonal), Kir2.1(Zenbio), Cx40 (GeneTex), TGF-β1(Santa cruz), smad (Zenbio), p-smad (Zenbio) at 4 ◦C overnight.

    Techniques: Activation Assay, Western Blot, Quantitation Assay, Expressing