Structured Review

Abcam kchip2
Relevant pathways in the regulation of <t>KChIP2.</t> The results showed an increasing level of NF-κB p65 and a decreasing level of IκBα after CRP treatment (10 μg/ml) (A, D) and the same level of p-ERK and p-JNK between control group and treatment group (B, E) (# P
Kchip2, supplied by Abcam, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kchip2/product/Abcam
Average 85 stars, based on 3 article reviews
Price from $9.99 to $1999.99
kchip2 - by Bioz Stars, 2022-12
85/100 stars

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1) Product Images from "Effects of C-reactive protein on K+ channel interaction protein 2 in cardiomyocytes"

Article Title: Effects of C-reactive protein on K+ channel interaction protein 2 in cardiomyocytes

Journal: American Journal of Translational Research

doi:

Relevant pathways in the regulation of KChIP2. The results showed an increasing level of NF-κB p65 and a decreasing level of IκBα after CRP treatment (10 μg/ml) (A, D) and the same level of p-ERK and p-JNK between control group and treatment group (B, E) (# P
Figure Legend Snippet: Relevant pathways in the regulation of KChIP2. The results showed an increasing level of NF-κB p65 and a decreasing level of IκBα after CRP treatment (10 μg/ml) (A, D) and the same level of p-ERK and p-JNK between control group and treatment group (B, E) (# P

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  • 90
    Abcam anti kchip2 antibody
    Activation of p38 MAPK, p44/42 MAPK, and NF-κB signaling pathways downregulated I to,f -related protein and mRNA. ( A and B ) Representative immunoblots ( A ) and average data ( B ) of Kv4.2, Kv4.3, <t>KChIP2,</t> and P-p38 MAPK in control, SB, IS, and IS plus SB groups ( n = 3 per group). ( C ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in control, SB, IS, and IS plus SB groups ( n = 3 per group). ( D and E ) Representative immunoblots ( D ) and average data ( E ) of Kv4.2, Kv4.3, KChIP2, and P-p44/42 MAPK in control, U0126, IS, and IS plus U0126 groups ( n = 3 per group). ( F ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in control, U0126, IS, and IS plus U0126 groups ( n = 3 per group). ( G and H ) Representative immunoblots ( G ) and average data ( H ) of Kv4.2, Kv4.3, KChIP2, and P-NF-κB in control, BAY, IS, and IS plus BAY groups ( n = 3 per group). ( I ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in control, BAY, IS, and IS plus BAY groups ( n = 3 per group). NRVMs in IS, IS plus SB, IS plus U0126, and IS plus BAY groups were treated with 10 μM IS. Data are presented as mean ± SEM. Statistical analysis was performed using 1-way ANOVA, followed by Bonferroni post hoc test. * P
    Anti Kchip2 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kchip2 antibody/product/Abcam
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kchip2 antibody - by Bioz Stars, 2022-12
    90/100 stars
      Buy from Supplier

    92
    Abcam anti kchip2 antibody s60 73
    Kvβ2 promotes Kv1 and Kv4 surface expression in cardiac myocytes. ( A ) Differential interference contrast (DIC) and confocal images showing Kvβ2-associated fluorescence (red) in isolated cardiac myocytes from wild type (wt) and Kvβ2 −/− animals. Nuclei (dapi) are shown in blue. ( B ) Western blots showing immunoreactive bands for Kv pore-forming and auxiliary subunits at respective predicted molecular weights as indicated in heart lysates from wt (n = 3) and Kvβ2 −/− (n = 3) animals. As a representative loading control, immunoreactive bands for GAPDH are shown for each lane of Kv1.5 blot. ( C ) Summarized densitometric data for Kv1.4, Kv1.5, Kv2.1, Kv4.2, Kv4.3, Kvβ1.1, Kvβ1.2, Kvβ2, and <t>KChIP2</t> proteins in heart lysates of wt animals. Data are normalized to GAPDH (run for each blot) and expressed relative to wt controls. *P
    Anti Kchip2 Antibody S60 73, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kchip2 antibody s60 73/product/Abcam
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti kchip2 antibody s60 73 - by Bioz Stars, 2022-12
    92/100 stars
      Buy from Supplier

    Image Search Results


    Activation of p38 MAPK, p44/42 MAPK, and NF-κB signaling pathways downregulated I to,f -related protein and mRNA. ( A and B ) Representative immunoblots ( A ) and average data ( B ) of Kv4.2, Kv4.3, KChIP2, and P-p38 MAPK in control, SB, IS, and IS plus SB groups ( n = 3 per group). ( C ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in control, SB, IS, and IS plus SB groups ( n = 3 per group). ( D and E ) Representative immunoblots ( D ) and average data ( E ) of Kv4.2, Kv4.3, KChIP2, and P-p44/42 MAPK in control, U0126, IS, and IS plus U0126 groups ( n = 3 per group). ( F ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in control, U0126, IS, and IS plus U0126 groups ( n = 3 per group). ( G and H ) Representative immunoblots ( G ) and average data ( H ) of Kv4.2, Kv4.3, KChIP2, and P-NF-κB in control, BAY, IS, and IS plus BAY groups ( n = 3 per group). ( I ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in control, BAY, IS, and IS plus BAY groups ( n = 3 per group). NRVMs in IS, IS plus SB, IS plus U0126, and IS plus BAY groups were treated with 10 μM IS. Data are presented as mean ± SEM. Statistical analysis was performed using 1-way ANOVA, followed by Bonferroni post hoc test. * P

    Journal: JCI Insight

    Article Title: Indoxyl sulfate reduces Ito,f by activating ROS/MAPK and NF- κB signaling pathways

    doi: 10.1172/jci.insight.145475

    Figure Lengend Snippet: Activation of p38 MAPK, p44/42 MAPK, and NF-κB signaling pathways downregulated I to,f -related protein and mRNA. ( A and B ) Representative immunoblots ( A ) and average data ( B ) of Kv4.2, Kv4.3, KChIP2, and P-p38 MAPK in control, SB, IS, and IS plus SB groups ( n = 3 per group). ( C ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in control, SB, IS, and IS plus SB groups ( n = 3 per group). ( D and E ) Representative immunoblots ( D ) and average data ( E ) of Kv4.2, Kv4.3, KChIP2, and P-p44/42 MAPK in control, U0126, IS, and IS plus U0126 groups ( n = 3 per group). ( F ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in control, U0126, IS, and IS plus U0126 groups ( n = 3 per group). ( G and H ) Representative immunoblots ( G ) and average data ( H ) of Kv4.2, Kv4.3, KChIP2, and P-NF-κB in control, BAY, IS, and IS plus BAY groups ( n = 3 per group). ( I ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in control, BAY, IS, and IS plus BAY groups ( n = 3 per group). NRVMs in IS, IS plus SB, IS plus U0126, and IS plus BAY groups were treated with 10 μM IS. Data are presented as mean ± SEM. Statistical analysis was performed using 1-way ANOVA, followed by Bonferroni post hoc test. * P

    Article Snippet: The cells were then incubated with primary antibodies including anti-cTNI antibody (1:100, 21652-1-AP, Proteintech), anti-Kv4.2 antibody (1:100, 21298-1-AP, Proteintech), anti-KChIP2 antibody (1:100, ab88542, Abcam), and anti–NF-κB p65 antibody (1:400, 8242S, Cell Signaling Technology) at 4°C overnight.

    Techniques: Activation Assay, Western Blot

    IS reduced I to,f -related protein expression levels and current densities in vitro. ( A and B ) Representative immunoblots ( A ) and average data ( B ) of Kv4.2, Kv4.3, and KChIP2 proteins in NRVMs treated with different concentrations of IS ( n =3 per group). ( C ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in NRVMs treated with different concentrations of IS ( n = 3 per group). ( D and E ) Representative immunofluorescence images of Kv4.2 ( D ) and KChIP2 ( E ) proteins in NRVMs. Scale bar: 50 μm. ( F and G ) Representative I to,f traces ( F ) and average I to,f densities (peak minus steady state) versus membrane potentials ( G ) in NRVMs treated with different concentrations of IS ( n = 5 per group).The inset in F shows the voltage-clamp protocol. ( H ) Average time constants (τ) of decay of I to,f at +60 mV in NRVMs ( n = 5 per group). ( I and J ) Average values of constants (k) of activation ( I ) and half-maximal voltage of activation(V 0.5 , act) ( J ) in NRVMs ( n = 5 per group). ( K ) Voltage-dependent activation curves of I to,f in NRVMs ( n = 5 per group). Data are presented as mean ± SEM. Statistical analysis was performed using 1-way ANOVA followed by Bonferroni post hoc test. * P

    Journal: JCI Insight

    Article Title: Indoxyl sulfate reduces Ito,f by activating ROS/MAPK and NF- κB signaling pathways

    doi: 10.1172/jci.insight.145475

    Figure Lengend Snippet: IS reduced I to,f -related protein expression levels and current densities in vitro. ( A and B ) Representative immunoblots ( A ) and average data ( B ) of Kv4.2, Kv4.3, and KChIP2 proteins in NRVMs treated with different concentrations of IS ( n =3 per group). ( C ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in NRVMs treated with different concentrations of IS ( n = 3 per group). ( D and E ) Representative immunofluorescence images of Kv4.2 ( D ) and KChIP2 ( E ) proteins in NRVMs. Scale bar: 50 μm. ( F and G ) Representative I to,f traces ( F ) and average I to,f densities (peak minus steady state) versus membrane potentials ( G ) in NRVMs treated with different concentrations of IS ( n = 5 per group).The inset in F shows the voltage-clamp protocol. ( H ) Average time constants (τ) of decay of I to,f at +60 mV in NRVMs ( n = 5 per group). ( I and J ) Average values of constants (k) of activation ( I ) and half-maximal voltage of activation(V 0.5 , act) ( J ) in NRVMs ( n = 5 per group). ( K ) Voltage-dependent activation curves of I to,f in NRVMs ( n = 5 per group). Data are presented as mean ± SEM. Statistical analysis was performed using 1-way ANOVA followed by Bonferroni post hoc test. * P

    Article Snippet: The cells were then incubated with primary antibodies including anti-cTNI antibody (1:100, 21652-1-AP, Proteintech), anti-Kv4.2 antibody (1:100, 21298-1-AP, Proteintech), anti-KChIP2 antibody (1:100, ab88542, Abcam), and anti–NF-κB p65 antibody (1:400, 8242S, Cell Signaling Technology) at 4°C overnight.

    Techniques: Expressing, In Vitro, Western Blot, Immunofluorescence, Activation Assay

    ROS production was involved in IS-induced reductions of I to,f -related proteins. ( A and D ) Measurements of ROS productions based on DHE fluorescence in rat hearts. ( A ) Representative images of DHE immunofluorescence. Scale bar: 200 μm. ( D ) Relative ROS fluorescence intensities in sham, CKD, and CKD plus BB536 groups ( n = 5 per group), and in vehicle and IS treatment groups ( n = 6 per group). ( B and E ) Measurements of ROS productions based on flow cytometry in NRVMs treated with different concentrations of IS. ( B ) Representative flow cytometric histograms in NRVMs. ( E ) Relative ROS fluorescence intensities in NRVMs ( n = 5 per group). ( C and F ) NAC reversed ROS production induced by IS in NRVMs. ( C ) Representative flow cytometric histograms in 4 groups. ( F ) Relative ROS fluorescence intensities detected by flow cytometry in 4 groups ( n = 5 per group). ( G and H ) Representative immunoblots of NOX2 proteins in sham, CKD, and CKD plus BB536 groups ( n = 5 per group) ( G ), and in vehicle and IS treatment groups ( n = 6 per group) ( H ). ( I ) Average immunoblots data of NOX2 proteins in the hearts of rats. ( J and M ) Representative immunoblots ( J ) and average data ( M ) of NOX2 proteins in NRVMs treated with different concentrations of IS ( n = 3 per group). ( K and N ) Representative immunoblots ( K ) and average data ( N ) of NOX2 proteins in control, DPI, IS, and IS plus DPI groups ( n = 3 per group). ( L and O ) Representative immunoblots ( L ) and average data ( O ) of NOX2 proteins in control, APO, IS, and IS plus APO groups ( n = 3 per group). ( P and Q ) Representative immunoblots ( P ) and average data ( Q ) of Kv4.2, Kv4.3, and KChIP2 proteins in control, NAC, IS, and IS plus NAC groups ( n = 3 per group). ( R ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in control, NAC, IS, and IS plus NAC groups ( n = 3 per group). NRVMs in IS and IS plus NAC groups were treated with 10 μM IS. Data are presented as mean ± SEM. Statistical analysis was performed using 2-tailed Student’s t test ( D and I ) and 1-way ANOVA followed by Bonferroni post hoc test ( D – F , I , M – O , Q , and R ). * P

    Journal: JCI Insight

    Article Title: Indoxyl sulfate reduces Ito,f by activating ROS/MAPK and NF- κB signaling pathways

    doi: 10.1172/jci.insight.145475

    Figure Lengend Snippet: ROS production was involved in IS-induced reductions of I to,f -related proteins. ( A and D ) Measurements of ROS productions based on DHE fluorescence in rat hearts. ( A ) Representative images of DHE immunofluorescence. Scale bar: 200 μm. ( D ) Relative ROS fluorescence intensities in sham, CKD, and CKD plus BB536 groups ( n = 5 per group), and in vehicle and IS treatment groups ( n = 6 per group). ( B and E ) Measurements of ROS productions based on flow cytometry in NRVMs treated with different concentrations of IS. ( B ) Representative flow cytometric histograms in NRVMs. ( E ) Relative ROS fluorescence intensities in NRVMs ( n = 5 per group). ( C and F ) NAC reversed ROS production induced by IS in NRVMs. ( C ) Representative flow cytometric histograms in 4 groups. ( F ) Relative ROS fluorescence intensities detected by flow cytometry in 4 groups ( n = 5 per group). ( G and H ) Representative immunoblots of NOX2 proteins in sham, CKD, and CKD plus BB536 groups ( n = 5 per group) ( G ), and in vehicle and IS treatment groups ( n = 6 per group) ( H ). ( I ) Average immunoblots data of NOX2 proteins in the hearts of rats. ( J and M ) Representative immunoblots ( J ) and average data ( M ) of NOX2 proteins in NRVMs treated with different concentrations of IS ( n = 3 per group). ( K and N ) Representative immunoblots ( K ) and average data ( N ) of NOX2 proteins in control, DPI, IS, and IS plus DPI groups ( n = 3 per group). ( L and O ) Representative immunoblots ( L ) and average data ( O ) of NOX2 proteins in control, APO, IS, and IS plus APO groups ( n = 3 per group). ( P and Q ) Representative immunoblots ( P ) and average data ( Q ) of Kv4.2, Kv4.3, and KChIP2 proteins in control, NAC, IS, and IS plus NAC groups ( n = 3 per group). ( R ) Relative mRNA expressions for Kv4.2, Kv4.3, and KChIP2 in control, NAC, IS, and IS plus NAC groups ( n = 3 per group). NRVMs in IS and IS plus NAC groups were treated with 10 μM IS. Data are presented as mean ± SEM. Statistical analysis was performed using 2-tailed Student’s t test ( D and I ) and 1-way ANOVA followed by Bonferroni post hoc test ( D – F , I , M – O , Q , and R ). * P

    Article Snippet: The cells were then incubated with primary antibodies including anti-cTNI antibody (1:100, 21652-1-AP, Proteintech), anti-Kv4.2 antibody (1:100, 21298-1-AP, Proteintech), anti-KChIP2 antibody (1:100, ab88542, Abcam), and anti–NF-κB p65 antibody (1:400, 8242S, Cell Signaling Technology) at 4°C overnight.

    Techniques: Fluorescence, Immunofluorescence, Flow Cytometry, Western Blot

    I to,f -related proteins were downregulated in CKD rats with a high levels of IS. ( A ) Flow chart of animal experiments. ( B and D ) Determinations of IS levels in serum ( B ) and heart tissues ( D ) at 8 weeks after right nephrectomy ( n = 5 per group). ( C and E ) Measurements of IS levels in serum ( C ) and heart tissues ( E ) after 8 weeks of IS treatment ( n = 6 per group). ( F and I ) Representative immunoblots ( F ) and average data ( I ) of Kv4.2, Kv4.3, and KChIP2 proteins in vehicle and IS treatment groups ( n = 6 per group). ( G and H ) Representative immunoblots ( G ) and average data ( H ) of Kv4.2, Kv4.3, and KChIP2 proteins in sham, CKD, and CKD plus BB536 groups ( n = 5 per group). ( J ) Representative IHC images of Kv4.2 and Kv4.3 proteins in rats. Scale bar: 100 μm. Data are presented as mean ± SEM. Statistical analysis was performed using 2-tailed Student’s t test ( C , E , and I ) and 1-way ANOVA followed by Bonferroni post hoc test ( B , D , and H ). * P

    Journal: JCI Insight

    Article Title: Indoxyl sulfate reduces Ito,f by activating ROS/MAPK and NF- κB signaling pathways

    doi: 10.1172/jci.insight.145475

    Figure Lengend Snippet: I to,f -related proteins were downregulated in CKD rats with a high levels of IS. ( A ) Flow chart of animal experiments. ( B and D ) Determinations of IS levels in serum ( B ) and heart tissues ( D ) at 8 weeks after right nephrectomy ( n = 5 per group). ( C and E ) Measurements of IS levels in serum ( C ) and heart tissues ( E ) after 8 weeks of IS treatment ( n = 6 per group). ( F and I ) Representative immunoblots ( F ) and average data ( I ) of Kv4.2, Kv4.3, and KChIP2 proteins in vehicle and IS treatment groups ( n = 6 per group). ( G and H ) Representative immunoblots ( G ) and average data ( H ) of Kv4.2, Kv4.3, and KChIP2 proteins in sham, CKD, and CKD plus BB536 groups ( n = 5 per group). ( J ) Representative IHC images of Kv4.2 and Kv4.3 proteins in rats. Scale bar: 100 μm. Data are presented as mean ± SEM. Statistical analysis was performed using 2-tailed Student’s t test ( C , E , and I ) and 1-way ANOVA followed by Bonferroni post hoc test ( B , D , and H ). * P

    Article Snippet: The cells were then incubated with primary antibodies including anti-cTNI antibody (1:100, 21652-1-AP, Proteintech), anti-Kv4.2 antibody (1:100, 21298-1-AP, Proteintech), anti-KChIP2 antibody (1:100, ab88542, Abcam), and anti–NF-κB p65 antibody (1:400, 8242S, Cell Signaling Technology) at 4°C overnight.

    Techniques: Western Blot, Immunohistochemistry

    Kvβ2 promotes Kv1 and Kv4 surface expression in cardiac myocytes. ( A ) Differential interference contrast (DIC) and confocal images showing Kvβ2-associated fluorescence (red) in isolated cardiac myocytes from wild type (wt) and Kvβ2 −/− animals. Nuclei (dapi) are shown in blue. ( B ) Western blots showing immunoreactive bands for Kv pore-forming and auxiliary subunits at respective predicted molecular weights as indicated in heart lysates from wt (n = 3) and Kvβ2 −/− (n = 3) animals. As a representative loading control, immunoreactive bands for GAPDH are shown for each lane of Kv1.5 blot. ( C ) Summarized densitometric data for Kv1.4, Kv1.5, Kv2.1, Kv4.2, Kv4.3, Kvβ1.1, Kvβ1.2, Kvβ2, and KChIP2 proteins in heart lysates of wt animals. Data are normalized to GAPDH (run for each blot) and expressed relative to wt controls. *P

    Journal: Journal of molecular and cellular cardiology

    Article Title: Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits

    doi: 10.1016/j.yjmcc.2019.09.013

    Figure Lengend Snippet: Kvβ2 promotes Kv1 and Kv4 surface expression in cardiac myocytes. ( A ) Differential interference contrast (DIC) and confocal images showing Kvβ2-associated fluorescence (red) in isolated cardiac myocytes from wild type (wt) and Kvβ2 −/− animals. Nuclei (dapi) are shown in blue. ( B ) Western blots showing immunoreactive bands for Kv pore-forming and auxiliary subunits at respective predicted molecular weights as indicated in heart lysates from wt (n = 3) and Kvβ2 −/− (n = 3) animals. As a representative loading control, immunoreactive bands for GAPDH are shown for each lane of Kv1.5 blot. ( C ) Summarized densitometric data for Kv1.4, Kv1.5, Kv2.1, Kv4.2, Kv4.3, Kvβ1.1, Kvβ1.2, Kvβ2, and KChIP2 proteins in heart lysates of wt animals. Data are normalized to GAPDH (run for each blot) and expressed relative to wt controls. *P

    Article Snippet: Blots were probed overnight at 4°C with anti-Kv1.4 (NeuroMab; L13/31, 10 μg/ml) [ ], anti-Kv1.5 (Alomone; APC-004; 6 μg/ml) [ , ], anti-Kv2.1 (NeuroMab; 80/21,10 μg/ml) [ ], anti-Kv4.2 (NeuroMab; L28/4, 10 μg/ml) [ , ], anti-Kv4.3 (Alomone; APC-017, 4 μg/ml) [ ], anti-Kvβ2 (NeuroMab; K17/70, 10 μg/ml) [ ], anti-Kvβ1.1 (NeuroMab; K9/40; 10 μg/ml) [ ], anti-KChIP2 (Abcam; ab99041, 10 μg/ml) [ ], anti-pan cadherin (Santa Cruz; sc-1499, 1 μg/ml), and anti-GAPDH (Millipore; MAB374; 1 μg/ml).

    Techniques: Expressing, Fluorescence, Isolation, Western Blot