Structured Review

NeuroMab kchip2 pan kchip
Auxiliary subunits regulate BBS-Kv4.2 surface expression in HEK 293T cells. (A) Auxiliary subunits were shown to increase BBS-Kv4.2 membrane expression in HEK 293T cells via western blot analysis. Cells transfected with BBS-Kv4.2 alone or together with DPP6 or <t>KChIP2</t> were processed for surface biotinylation. (B) Surface labeling experiments show that auxiliary subunits facilitate BBS-Kv4.2 membrane localization in HEK 293T cells. Cells transfected with BBS-Kv4.2 alone or together with DPP6 and KChIP2 were incubated with RhBTX at 17°C for 30 min. Cells were fixed, permeabilized and stained with anti-Myc antibody. Co-transfection with DPP6 and KChIP2 increased surface BBS-Kv4.2 expression. Scale bar: 10 μm. (C) Graphical representation of (B) and Figure S2. The surface stain intensity of S3-S4 BBS-Kv4.2 (BBS-Kv4.2–285) is significantly higher than that of S1-S2 BBS-Kv4.2 (BBS-Kv4.2–220). n = 15 cells for each group. ***p < 0.001 vs alone, #p < 0.05, ###p < 0.001 vs BBS-Kv4.2–220. (D) KChIP2 and DPP6 auxiliary subunits increase Kv4.2 and BBS-Kv4.2 current density. Left, Kv4.2 and BBS-Kv4.2 current traces. Vertical and horizontal scale bars correspond to 100 pA/pF and 100 ms respectively. Right, current density for each construct co-expressed with DPP6 or KChIP2. BBS-Kv4.2 alone exhibits a decreased current density compared to that of Kv4.2 but the current densities of both constructs are similarly increased by auxiliary subunits.
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1) Product Images from "A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking"

Article Title: A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking

Journal: Molecular and cellular neurosciences

doi: 10.1016/j.mcn.2019.06.007

Auxiliary subunits regulate BBS-Kv4.2 surface expression in HEK 293T cells. (A) Auxiliary subunits were shown to increase BBS-Kv4.2 membrane expression in HEK 293T cells via western blot analysis. Cells transfected with BBS-Kv4.2 alone or together with DPP6 or KChIP2 were processed for surface biotinylation. (B) Surface labeling experiments show that auxiliary subunits facilitate BBS-Kv4.2 membrane localization in HEK 293T cells. Cells transfected with BBS-Kv4.2 alone or together with DPP6 and KChIP2 were incubated with RhBTX at 17°C for 30 min. Cells were fixed, permeabilized and stained with anti-Myc antibody. Co-transfection with DPP6 and KChIP2 increased surface BBS-Kv4.2 expression. Scale bar: 10 μm. (C) Graphical representation of (B) and Figure S2. The surface stain intensity of S3-S4 BBS-Kv4.2 (BBS-Kv4.2–285) is significantly higher than that of S1-S2 BBS-Kv4.2 (BBS-Kv4.2–220). n = 15 cells for each group. ***p < 0.001 vs alone, #p < 0.05, ###p < 0.001 vs BBS-Kv4.2–220. (D) KChIP2 and DPP6 auxiliary subunits increase Kv4.2 and BBS-Kv4.2 current density. Left, Kv4.2 and BBS-Kv4.2 current traces. Vertical and horizontal scale bars correspond to 100 pA/pF and 100 ms respectively. Right, current density for each construct co-expressed with DPP6 or KChIP2. BBS-Kv4.2 alone exhibits a decreased current density compared to that of Kv4.2 but the current densities of both constructs are similarly increased by auxiliary subunits.
Figure Legend Snippet: Auxiliary subunits regulate BBS-Kv4.2 surface expression in HEK 293T cells. (A) Auxiliary subunits were shown to increase BBS-Kv4.2 membrane expression in HEK 293T cells via western blot analysis. Cells transfected with BBS-Kv4.2 alone or together with DPP6 or KChIP2 were processed for surface biotinylation. (B) Surface labeling experiments show that auxiliary subunits facilitate BBS-Kv4.2 membrane localization in HEK 293T cells. Cells transfected with BBS-Kv4.2 alone or together with DPP6 and KChIP2 were incubated with RhBTX at 17°C for 30 min. Cells were fixed, permeabilized and stained with anti-Myc antibody. Co-transfection with DPP6 and KChIP2 increased surface BBS-Kv4.2 expression. Scale bar: 10 μm. (C) Graphical representation of (B) and Figure S2. The surface stain intensity of S3-S4 BBS-Kv4.2 (BBS-Kv4.2–285) is significantly higher than that of S1-S2 BBS-Kv4.2 (BBS-Kv4.2–220). n = 15 cells for each group. ***p < 0.001 vs alone, #p < 0.05, ###p < 0.001 vs BBS-Kv4.2–220. (D) KChIP2 and DPP6 auxiliary subunits increase Kv4.2 and BBS-Kv4.2 current density. Left, Kv4.2 and BBS-Kv4.2 current traces. Vertical and horizontal scale bars correspond to 100 pA/pF and 100 ms respectively. Right, current density for each construct co-expressed with DPP6 or KChIP2. BBS-Kv4.2 alone exhibits a decreased current density compared to that of Kv4.2 but the current densities of both constructs are similarly increased by auxiliary subunits.

Techniques Used: Expressing, Western Blot, Transfection, Labeling, Incubation, Staining, Cotransfection, Construct

Biophysical properties of Kv4.2 and BBS-Kv4.2 voltage gated K + currents.
Figure Legend Snippet: Biophysical properties of Kv4.2 and BBS-Kv4.2 voltage gated K + currents.

Techniques Used:


Structured Review

NeuroMab kchip2 pan kchip
Auxiliary subunits regulate BBS-Kv4.2 surface expression in HEK 293T cells. (A) Auxiliary subunits were shown to increase BBS-Kv4.2 membrane expression in HEK 293T cells via western blot analysis. Cells transfected with BBS-Kv4.2 alone or together with DPP6 or <t>KChIP2</t> were processed for surface biotinylation. (B) Surface labeling experiments show that auxiliary subunits facilitate BBS-Kv4.2 membrane localization in HEK 293T cells. Cells transfected with BBS-Kv4.2 alone or together with DPP6 and KChIP2 were incubated with RhBTX at 17°C for 30 min. Cells were fixed, permeabilized and stained with anti-Myc antibody. Co-transfection with DPP6 and KChIP2 increased surface BBS-Kv4.2 expression. Scale bar: 10 μm. (C) Graphical representation of (B) and Figure S2. The surface stain intensity of S3-S4 BBS-Kv4.2 (BBS-Kv4.2–285) is significantly higher than that of S1-S2 BBS-Kv4.2 (BBS-Kv4.2–220). n = 15 cells for each group. ***p < 0.001 vs alone, #p < 0.05, ###p < 0.001 vs BBS-Kv4.2–220. (D) KChIP2 and DPP6 auxiliary subunits increase Kv4.2 and BBS-Kv4.2 current density. Left, Kv4.2 and BBS-Kv4.2 current traces. Vertical and horizontal scale bars correspond to 100 pA/pF and 100 ms respectively. Right, current density for each construct co-expressed with DPP6 or KChIP2. BBS-Kv4.2 alone exhibits a decreased current density compared to that of Kv4.2 but the current densities of both constructs are similarly increased by auxiliary subunits.
Kchip2 Pan Kchip, supplied by NeuroMab, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Price from $9.99 to $1999.99
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Images

1) Product Images from "A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking"

Article Title: A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking

Journal: Molecular and cellular neurosciences

doi: 10.1016/j.mcn.2019.06.007

Auxiliary subunits regulate BBS-Kv4.2 surface expression in HEK 293T cells. (A) Auxiliary subunits were shown to increase BBS-Kv4.2 membrane expression in HEK 293T cells via western blot analysis. Cells transfected with BBS-Kv4.2 alone or together with DPP6 or KChIP2 were processed for surface biotinylation. (B) Surface labeling experiments show that auxiliary subunits facilitate BBS-Kv4.2 membrane localization in HEK 293T cells. Cells transfected with BBS-Kv4.2 alone or together with DPP6 and KChIP2 were incubated with RhBTX at 17°C for 30 min. Cells were fixed, permeabilized and stained with anti-Myc antibody. Co-transfection with DPP6 and KChIP2 increased surface BBS-Kv4.2 expression. Scale bar: 10 μm. (C) Graphical representation of (B) and Figure S2. The surface stain intensity of S3-S4 BBS-Kv4.2 (BBS-Kv4.2–285) is significantly higher than that of S1-S2 BBS-Kv4.2 (BBS-Kv4.2–220). n = 15 cells for each group. ***p < 0.001 vs alone, #p < 0.05, ###p < 0.001 vs BBS-Kv4.2–220. (D) KChIP2 and DPP6 auxiliary subunits increase Kv4.2 and BBS-Kv4.2 current density. Left, Kv4.2 and BBS-Kv4.2 current traces. Vertical and horizontal scale bars correspond to 100 pA/pF and 100 ms respectively. Right, current density for each construct co-expressed with DPP6 or KChIP2. BBS-Kv4.2 alone exhibits a decreased current density compared to that of Kv4.2 but the current densities of both constructs are similarly increased by auxiliary subunits.
Figure Legend Snippet: Auxiliary subunits regulate BBS-Kv4.2 surface expression in HEK 293T cells. (A) Auxiliary subunits were shown to increase BBS-Kv4.2 membrane expression in HEK 293T cells via western blot analysis. Cells transfected with BBS-Kv4.2 alone or together with DPP6 or KChIP2 were processed for surface biotinylation. (B) Surface labeling experiments show that auxiliary subunits facilitate BBS-Kv4.2 membrane localization in HEK 293T cells. Cells transfected with BBS-Kv4.2 alone or together with DPP6 and KChIP2 were incubated with RhBTX at 17°C for 30 min. Cells were fixed, permeabilized and stained with anti-Myc antibody. Co-transfection with DPP6 and KChIP2 increased surface BBS-Kv4.2 expression. Scale bar: 10 μm. (C) Graphical representation of (B) and Figure S2. The surface stain intensity of S3-S4 BBS-Kv4.2 (BBS-Kv4.2–285) is significantly higher than that of S1-S2 BBS-Kv4.2 (BBS-Kv4.2–220). n = 15 cells for each group. ***p < 0.001 vs alone, #p < 0.05, ###p < 0.001 vs BBS-Kv4.2–220. (D) KChIP2 and DPP6 auxiliary subunits increase Kv4.2 and BBS-Kv4.2 current density. Left, Kv4.2 and BBS-Kv4.2 current traces. Vertical and horizontal scale bars correspond to 100 pA/pF and 100 ms respectively. Right, current density for each construct co-expressed with DPP6 or KChIP2. BBS-Kv4.2 alone exhibits a decreased current density compared to that of Kv4.2 but the current densities of both constructs are similarly increased by auxiliary subunits.

Techniques Used: Expressing, Western Blot, Transfection, Labeling, Incubation, Staining, Cotransfection, Construct

Biophysical properties of Kv4.2 and BBS-Kv4.2 voltage gated K + currents.
Figure Legend Snippet: Biophysical properties of Kv4.2 and BBS-Kv4.2 voltage gated K + currents.

Techniques Used:

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    NeuroMab kchip2 pan kchip
    Auxiliary subunits regulate BBS-Kv4.2 surface expression in HEK 293T cells. (A) Auxiliary subunits were shown to increase BBS-Kv4.2 membrane expression in HEK 293T cells via western blot analysis. Cells transfected with BBS-Kv4.2 alone or together with DPP6 or <t>KChIP2</t> were processed for surface biotinylation. (B) Surface labeling experiments show that auxiliary subunits facilitate BBS-Kv4.2 membrane localization in HEK 293T cells. Cells transfected with BBS-Kv4.2 alone or together with DPP6 and KChIP2 were incubated with RhBTX at 17°C for 30 min. Cells were fixed, permeabilized and stained with anti-Myc antibody. Co-transfection with DPP6 and KChIP2 increased surface BBS-Kv4.2 expression. Scale bar: 10 μm. (C) Graphical representation of (B) and Figure S2. The surface stain intensity of S3-S4 BBS-Kv4.2 (BBS-Kv4.2–285) is significantly higher than that of S1-S2 BBS-Kv4.2 (BBS-Kv4.2–220). n = 15 cells for each group. ***p < 0.001 vs alone, #p < 0.05, ###p < 0.001 vs BBS-Kv4.2–220. (D) KChIP2 and DPP6 auxiliary subunits increase Kv4.2 and BBS-Kv4.2 current density. Left, Kv4.2 and BBS-Kv4.2 current traces. Vertical and horizontal scale bars correspond to 100 pA/pF and 100 ms respectively. Right, current density for each construct co-expressed with DPP6 or KChIP2. BBS-Kv4.2 alone exhibits a decreased current density compared to that of Kv4.2 but the current densities of both constructs are similarly increased by auxiliary subunits.
    Kchip2 Pan Kchip, supplied by NeuroMab, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kchip2 pan kchip/product/NeuroMab
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kchip2 pan kchip - by Bioz Stars, 2023-02
    86/100 stars
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    Auxiliary subunits regulate BBS-Kv4.2 surface expression in HEK 293T cells. (A) Auxiliary subunits were shown to increase BBS-Kv4.2 membrane expression in HEK 293T cells via western blot analysis. Cells transfected with BBS-Kv4.2 alone or together with DPP6 or KChIP2 were processed for surface biotinylation. (B) Surface labeling experiments show that auxiliary subunits facilitate BBS-Kv4.2 membrane localization in HEK 293T cells. Cells transfected with BBS-Kv4.2 alone or together with DPP6 and KChIP2 were incubated with RhBTX at 17°C for 30 min. Cells were fixed, permeabilized and stained with anti-Myc antibody. Co-transfection with DPP6 and KChIP2 increased surface BBS-Kv4.2 expression. Scale bar: 10 μm. (C) Graphical representation of (B) and Figure S2. The surface stain intensity of S3-S4 BBS-Kv4.2 (BBS-Kv4.2–285) is significantly higher than that of S1-S2 BBS-Kv4.2 (BBS-Kv4.2–220). n = 15 cells for each group. ***p < 0.001 vs alone, #p < 0.05, ###p < 0.001 vs BBS-Kv4.2–220. (D) KChIP2 and DPP6 auxiliary subunits increase Kv4.2 and BBS-Kv4.2 current density. Left, Kv4.2 and BBS-Kv4.2 current traces. Vertical and horizontal scale bars correspond to 100 pA/pF and 100 ms respectively. Right, current density for each construct co-expressed with DPP6 or KChIP2. BBS-Kv4.2 alone exhibits a decreased current density compared to that of Kv4.2 but the current densities of both constructs are similarly increased by auxiliary subunits.

    Journal: Molecular and cellular neurosciences

    Article Title: A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking

    doi: 10.1016/j.mcn.2019.06.007

    Figure Lengend Snippet: Auxiliary subunits regulate BBS-Kv4.2 surface expression in HEK 293T cells. (A) Auxiliary subunits were shown to increase BBS-Kv4.2 membrane expression in HEK 293T cells via western blot analysis. Cells transfected with BBS-Kv4.2 alone or together with DPP6 or KChIP2 were processed for surface biotinylation. (B) Surface labeling experiments show that auxiliary subunits facilitate BBS-Kv4.2 membrane localization in HEK 293T cells. Cells transfected with BBS-Kv4.2 alone or together with DPP6 and KChIP2 were incubated with RhBTX at 17°C for 30 min. Cells were fixed, permeabilized and stained with anti-Myc antibody. Co-transfection with DPP6 and KChIP2 increased surface BBS-Kv4.2 expression. Scale bar: 10 μm. (C) Graphical representation of (B) and Figure S2. The surface stain intensity of S3-S4 BBS-Kv4.2 (BBS-Kv4.2–285) is significantly higher than that of S1-S2 BBS-Kv4.2 (BBS-Kv4.2–220). n = 15 cells for each group. ***p < 0.001 vs alone, #p < 0.05, ###p < 0.001 vs BBS-Kv4.2–220. (D) KChIP2 and DPP6 auxiliary subunits increase Kv4.2 and BBS-Kv4.2 current density. Left, Kv4.2 and BBS-Kv4.2 current traces. Vertical and horizontal scale bars correspond to 100 pA/pF and 100 ms respectively. Right, current density for each construct co-expressed with DPP6 or KChIP2. BBS-Kv4.2 alone exhibits a decreased current density compared to that of Kv4.2 but the current densities of both constructs are similarly increased by auxiliary subunits.

    Article Snippet: Kv4.2 (K57/1): Neuromab 75–016, 1:100 for staining, 1:2000 for western blot; Myc: Millipore, 05–419, 1:500 for staining, 1:5000 for western blot; DPP6: Abcam, ab41811, 1:1000 for western blot; KChIP2: pan KChIP, Neuromab, 75–006, 1:1000 for western blot; Actin: Sigma, A-1978, 1:10000 for western blot; Alexa Fluor 488 goat anti-mouse: Invitrogen, A-11029, 1:500; Alexa Fluor 680 goat anti-mouse: Invitrogen, A-21057, 1:10000; Alexa Fluor 680 goat anti-rabbit: Invitrogen, A-21076, 1:10000.

    Techniques: Expressing, Western Blot, Transfection, Labeling, Incubation, Staining, Cotransfection, Construct

    Biophysical properties of Kv4.2 and BBS-Kv4.2 voltage gated K + currents.

    Journal: Molecular and cellular neurosciences

    Article Title: A novel bungarotoxin binding site-tagged construct reveals MAPK-dependent Kv4.2 trafficking

    doi: 10.1016/j.mcn.2019.06.007

    Figure Lengend Snippet: Biophysical properties of Kv4.2 and BBS-Kv4.2 voltage gated K + currents.

    Article Snippet: Kv4.2 (K57/1): Neuromab 75–016, 1:100 for staining, 1:2000 for western blot; Myc: Millipore, 05–419, 1:500 for staining, 1:5000 for western blot; DPP6: Abcam, ab41811, 1:1000 for western blot; KChIP2: pan KChIP, Neuromab, 75–006, 1:1000 for western blot; Actin: Sigma, A-1978, 1:10000 for western blot; Alexa Fluor 488 goat anti-mouse: Invitrogen, A-11029, 1:500; Alexa Fluor 680 goat anti-mouse: Invitrogen, A-21057, 1:10000; Alexa Fluor 680 goat anti-rabbit: Invitrogen, A-21076, 1:10000.

    Techniques: