kb 3 1 cells  (ATCC)


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    ATCC kb 3 1 cells
    Kb 3 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    kb 3 1 cells  (ATCC)


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    ATCC kb 3 1 cells
    Kb 3 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human cervix carcinoma cell line kb 3 1  (ATCC)


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    ATCC human cervix carcinoma cell line kb 3 1
    Human Cervix Carcinoma Cell Line Kb 3 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    kb 3 1 cells  (ATCC)


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    ATCC kb 3 1 cells
    Analysis by (A) flow cytometry and (B) imaging flow cytometry confirms MSLN presence on the surface of <t>KB-3-1</t> cells compared to the MSLN-negative MCF-7 cells, as detected by an anti-MSLN antibody. (C, D) KB-3-1 cells ( top ) internalize AF488-labeled 1.4.1 (C) and AF488-labeled 2.4.1 (D), while MCF-7 cells show no specific binding or internalization (C bottom , D bottom ). Endosomes are detected by an AF647-conjuated antibody recognizing the EAA1 early endosomal marker. Yellow in the merged images indicate co-localization between AF488-1.4.1 or AF488-2.4.1 anti-MSLN engineered proteins (green) and EEA1 (red). Original magnification 40X. Co-localization is quantified by the Bright Detail Similarity (BDS) metric, with values near 1 indicating co-localization. KB-3-1 BDS = 1.31 and 0.919 for AF488-1.4.1 and AF488-2.4.1, respectively. BDS values are not quantifiable for the negative control cell line, due to insufficient fraction of negative control cell population staining for binding or internalization of engineered protein variants.
    Kb 3 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Fn3 proteins engineered to recognize tumor biomarker mesothelin internalize upon binding"

    Article Title: Fn3 proteins engineered to recognize tumor biomarker mesothelin internalize upon binding

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0197029

    Analysis by (A) flow cytometry and (B) imaging flow cytometry confirms MSLN presence on the surface of KB-3-1 cells compared to the MSLN-negative MCF-7 cells, as detected by an anti-MSLN antibody. (C, D) KB-3-1 cells ( top ) internalize AF488-labeled 1.4.1 (C) and AF488-labeled 2.4.1 (D), while MCF-7 cells show no specific binding or internalization (C bottom , D bottom ). Endosomes are detected by an AF647-conjuated antibody recognizing the EAA1 early endosomal marker. Yellow in the merged images indicate co-localization between AF488-1.4.1 or AF488-2.4.1 anti-MSLN engineered proteins (green) and EEA1 (red). Original magnification 40X. Co-localization is quantified by the Bright Detail Similarity (BDS) metric, with values near 1 indicating co-localization. KB-3-1 BDS = 1.31 and 0.919 for AF488-1.4.1 and AF488-2.4.1, respectively. BDS values are not quantifiable for the negative control cell line, due to insufficient fraction of negative control cell population staining for binding or internalization of engineered protein variants.
    Figure Legend Snippet: Analysis by (A) flow cytometry and (B) imaging flow cytometry confirms MSLN presence on the surface of KB-3-1 cells compared to the MSLN-negative MCF-7 cells, as detected by an anti-MSLN antibody. (C, D) KB-3-1 cells ( top ) internalize AF488-labeled 1.4.1 (C) and AF488-labeled 2.4.1 (D), while MCF-7 cells show no specific binding or internalization (C bottom , D bottom ). Endosomes are detected by an AF647-conjuated antibody recognizing the EAA1 early endosomal marker. Yellow in the merged images indicate co-localization between AF488-1.4.1 or AF488-2.4.1 anti-MSLN engineered proteins (green) and EEA1 (red). Original magnification 40X. Co-localization is quantified by the Bright Detail Similarity (BDS) metric, with values near 1 indicating co-localization. KB-3-1 BDS = 1.31 and 0.919 for AF488-1.4.1 and AF488-2.4.1, respectively. BDS values are not quantifiable for the negative control cell line, due to insufficient fraction of negative control cell population staining for binding or internalization of engineered protein variants.

    Techniques Used: Flow Cytometry, Imaging, Labeling, Binding Assay, Marker, Negative Control, Staining

    kb 3 1 cells  (ATCC)


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    ATCC kb 3 1 cells
    Kb 3 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human cervix carcinoma cell line kb 3 1  (ATCC)


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    ATCC human cervix carcinoma cell line kb 3 1
    Human Cervix Carcinoma Cell Line Kb 3 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cervical carcinoma derived kb 3 1 kb31 cell line  (ATCC)


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    ATCC cervical carcinoma derived kb 3 1 kb31 cell line
    Cervical Carcinoma Derived Kb 3 1 Kb31 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cervical carcinoma derived kb 3 1 kb31 cell line  (ATCC)


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    ATCC cervical carcinoma derived kb 3 1 kb31 cell line
    Cervical Carcinoma Derived Kb 3 1 Kb31 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cervical carcinoma derived kb 3 1 kb31 cell line  (ATCC)


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    ATCC cervical carcinoma derived kb 3 1 kb31 cell line
    Cervical Carcinoma Derived Kb 3 1 Kb31 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cervical carcinoma derived kb 3 1 kb31 cell line  (ATCC)


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    ATCC cervical carcinoma derived kb 3 1 kb31 cell line
    Cervical Carcinoma Derived Kb 3 1 Kb31 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cervical carcinoma derived kb 3 1 kb31 cell line  (ATCC)


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    ATCC cervical carcinoma derived kb 3 1 kb31 cell line
    Cervical Carcinoma Derived Kb 3 1 Kb31 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC kb 3 1 cells
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