kapa long range pcr  (Kapa Biosystems)

 
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    Kapa Biosystems kapa long range pcr
    Kapa Long Range Pcr, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa long range pcr/product/Kapa Biosystems
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kapa long range pcr - by Bioz Stars, 2020-08
    85/100 stars

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    Polymerase Chain Reaction:

    Article Title: Epitope-Tagged Pkhd1 Tracks the Processing, Secretion, and Localization of Fibrocystin
    Article Snippet: .. PCR was with Kapa Long–Range PCR (KAPABIOSYSTEMS). .. Northern blotting was done with 10 μg of total cellular RNA denatured in formaldehyde/formamide and run on a MOPS–formaldehyde 0.5% agarose gel, 50V overnight.

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    Kapa Biosystems xl pcr
    Electrophoretic mobility shift assays demonstrating binding of <t>SpdA2-His</t> to the palindromic sequence motif sps . Different <t>PCR</t> fragments, as schematically illustrated, were incubated with ~2 pmol purified SpdA2-His protein (+) or without SpdA2-His (−), run on a 6% Tris-acetate polyacrylamide gel and stained with EtBr. The gray arrow indicates the band corresponding to a PCR fragment (FTraR pSVH1 ) added as a negative control for binding specificity. Black arrows mark retarded DNA fragments. Interaction of SpdA2-His with the FA2 fragment resulted in several retarded bands (A) . Whereas SpdA2-His did not interact with the FA2-sps fragment (B) , SpdA2-His shifted fragment F1sps, containing a single palindromic sps sequence (C) . SpdA2-His also bound to the PCR fragment FSpdA up , containing a T insertion within the palindromic sequence sps (D) , but did not recognize FSpdA, lacking sps (E) .
    Xl Pcr, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    kapa long range hotstart dna polymerase  (Kapa Biosystems)
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    Kapa Biosystems kapa long range hotstart dna polymerase
    Electrophoretic mobility shift assays demonstrating binding of <t>SpdA2-His</t> to the palindromic sequence motif sps . Different <t>PCR</t> fragments, as schematically illustrated, were incubated with ~2 pmol purified SpdA2-His protein (+) or without SpdA2-His (−), run on a 6% Tris-acetate polyacrylamide gel and stained with EtBr. The gray arrow indicates the band corresponding to a PCR fragment (FTraR pSVH1 ) added as a negative control for binding specificity. Black arrows mark retarded DNA fragments. Interaction of SpdA2-His with the FA2 fragment resulted in several retarded bands (A) . Whereas SpdA2-His did not interact with the FA2-sps fragment (B) , SpdA2-His shifted fragment F1sps, containing a single palindromic sps sequence (C) . SpdA2-His also bound to the PCR fragment FSpdA up , containing a T insertion within the palindromic sequence sps (D) , but did not recognize FSpdA, lacking sps (E) .
    Kapa Long Range Hotstart Dna Polymerase, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    high fidelity pcr kapa hot start long range pcr kit kapa biosystems wilmington ma usa  (Kapa Biosystems)
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    Kapa Biosystems high fidelity pcr kapa hot start long range pcr kit kapa biosystems wilmington ma usa
    Electrophoretic mobility shift assays demonstrating binding of <t>SpdA2-His</t> to the palindromic sequence motif sps . Different <t>PCR</t> fragments, as schematically illustrated, were incubated with ~2 pmol purified SpdA2-His protein (+) or without SpdA2-His (−), run on a 6% Tris-acetate polyacrylamide gel and stained with EtBr. The gray arrow indicates the band corresponding to a PCR fragment (FTraR pSVH1 ) added as a negative control for binding specificity. Black arrows mark retarded DNA fragments. Interaction of SpdA2-His with the FA2 fragment resulted in several retarded bands (A) . Whereas SpdA2-His did not interact with the FA2-sps fragment (B) , SpdA2-His shifted fragment F1sps, containing a single palindromic sps sequence (C) . SpdA2-His also bound to the PCR fragment FSpdA up , containing a T insertion within the palindromic sequence sps (D) , but did not recognize FSpdA, lacking sps (E) .
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    https://www.bioz.com/result/high fidelity pcr kapa hot start long range pcr kit kapa biosystems wilmington ma usa/product/Kapa Biosystems
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    kapa long range hot start dna polymerase kit  (Kapa Biosystems)
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    Kapa Biosystems kapa long range hot start dna polymerase kit
    Electrophoretic mobility shift assays demonstrating binding of <t>SpdA2-His</t> to the palindromic sequence motif sps . Different <t>PCR</t> fragments, as schematically illustrated, were incubated with ~2 pmol purified SpdA2-His protein (+) or without SpdA2-His (−), run on a 6% Tris-acetate polyacrylamide gel and stained with EtBr. The gray arrow indicates the band corresponding to a PCR fragment (FTraR pSVH1 ) added as a negative control for binding specificity. Black arrows mark retarded DNA fragments. Interaction of SpdA2-His with the FA2 fragment resulted in several retarded bands (A) . Whereas SpdA2-His did not interact with the FA2-sps fragment (B) , SpdA2-His shifted fragment F1sps, containing a single palindromic sps sequence (C) . SpdA2-His also bound to the PCR fragment FSpdA up , containing a T insertion within the palindromic sequence sps (D) , but did not recognize FSpdA, lacking sps (E) .
    Kapa Long Range Hot Start Dna Polymerase Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa long range hot start dna polymerase kit/product/Kapa Biosystems
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    Electrophoretic mobility shift assays demonstrating binding of SpdA2-His to the palindromic sequence motif sps . Different PCR fragments, as schematically illustrated, were incubated with ~2 pmol purified SpdA2-His protein (+) or without SpdA2-His (−), run on a 6% Tris-acetate polyacrylamide gel and stained with EtBr. The gray arrow indicates the band corresponding to a PCR fragment (FTraR pSVH1 ) added as a negative control for binding specificity. Black arrows mark retarded DNA fragments. Interaction of SpdA2-His with the FA2 fragment resulted in several retarded bands (A) . Whereas SpdA2-His did not interact with the FA2-sps fragment (B) , SpdA2-His shifted fragment F1sps, containing a single palindromic sps sequence (C) . SpdA2-His also bound to the PCR fragment FSpdA up , containing a T insertion within the palindromic sequence sps (D) , but did not recognize FSpdA, lacking sps (E) .

    Journal: Frontiers in Microbiology

    Article Title: The stability region of the Streptomyces lividans plasmid pIJ101 encodes a DNA-binding protein recognizing a highly conserved short palindromic sequence motif

    doi: 10.3389/fmicb.2014.00499

    Figure Lengend Snippet: Electrophoretic mobility shift assays demonstrating binding of SpdA2-His to the palindromic sequence motif sps . Different PCR fragments, as schematically illustrated, were incubated with ~2 pmol purified SpdA2-His protein (+) or without SpdA2-His (−), run on a 6% Tris-acetate polyacrylamide gel and stained with EtBr. The gray arrow indicates the band corresponding to a PCR fragment (FTraR pSVH1 ) added as a negative control for binding specificity. Black arrows mark retarded DNA fragments. Interaction of SpdA2-His with the FA2 fragment resulted in several retarded bands (A) . Whereas SpdA2-His did not interact with the FA2-sps fragment (B) , SpdA2-His shifted fragment F1sps, containing a single palindromic sps sequence (C) . SpdA2-His also bound to the PCR fragment FSpdA up , containing a T insertion within the palindromic sequence sps (D) , but did not recognize FSpdA, lacking sps (E) .

    Article Snippet: Construction of plasmids pIJ303ΔspdA2, pIJ303-sps, and pIJ303spdA2c To construct pIJ303Δ spdA2 we performed an XL-PCR (KAPA Long Range DNA Polymerase, Kapa Biosystems) with primers 303DelAl/303DelBu (Table ).

    Techniques: Electrophoretic Mobility Shift Assay, Binding Assay, Sequencing, Polymerase Chain Reaction, Incubation, Purification, Staining, Negative Control

    pIJ303 Δ spdA2 and pIJ303-sps do not accumulate single-stranded plasmid DNA . Approximately 10 6 protoplasts of S. lividans TK54 with respective plasmids were lysed in a 1% TA agarose gel containing 0.25% SDS (A) . Black arrows indicate chromosomal DNA, while the gray arrow marks ccc-DNA. The gel was either blotted in native state (B) to detect accumulation of single-stranded plasmid DNA, or denatured before blotting in order to detect also double-stranded plasmid DNA (C) . As a probe a 600 bp PCR-fragment corresponding to the dso of pIJ101 was used. Plasmid pIJ702, which lacks the sso , was used as a positive control for the accumulation of single-stranded plasmid DNA (white arrow). 1, pIJ303; 2, pIJ303-sps; 3, pIJ303Δ spdA2; 4, pIJ303spdA2c; 5, pIJ702; 6, plasmid free TK54.

    Journal: Frontiers in Microbiology

    Article Title: The stability region of the Streptomyces lividans plasmid pIJ101 encodes a DNA-binding protein recognizing a highly conserved short palindromic sequence motif

    doi: 10.3389/fmicb.2014.00499

    Figure Lengend Snippet: pIJ303 Δ spdA2 and pIJ303-sps do not accumulate single-stranded plasmid DNA . Approximately 10 6 protoplasts of S. lividans TK54 with respective plasmids were lysed in a 1% TA agarose gel containing 0.25% SDS (A) . Black arrows indicate chromosomal DNA, while the gray arrow marks ccc-DNA. The gel was either blotted in native state (B) to detect accumulation of single-stranded plasmid DNA, or denatured before blotting in order to detect also double-stranded plasmid DNA (C) . As a probe a 600 bp PCR-fragment corresponding to the dso of pIJ101 was used. Plasmid pIJ702, which lacks the sso , was used as a positive control for the accumulation of single-stranded plasmid DNA (white arrow). 1, pIJ303; 2, pIJ303-sps; 3, pIJ303Δ spdA2; 4, pIJ303spdA2c; 5, pIJ702; 6, plasmid free TK54.

    Article Snippet: Construction of plasmids pIJ303ΔspdA2, pIJ303-sps, and pIJ303spdA2c To construct pIJ303Δ spdA2 we performed an XL-PCR (KAPA Long Range DNA Polymerase, Kapa Biosystems) with primers 303DelAl/303DelBu (Table ).

    Techniques: Plasmid Preparation, Agarose Gel Electrophoresis, Countercurrent Chromatography, Polymerase Chain Reaction, Positive Control