Structured Review

Kapa Biosystems kapa hifi hot start dna polymerase
Kapa Hifi Hot Start Dna Polymerase, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kapa hifi hot start dna polymerase/product/Kapa Biosystems
Average 91 stars, based on 4 article reviews
Price from $9.99 to $1999.99
kapa hifi hot start dna polymerase - by Bioz Stars, 2020-08
91/100 stars

Images

Related Articles

Polymerase Chain Reaction:

Article Title: High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution
Article Snippet: .. The KAPA HiFi Hot Start DNA Polymerase (KAPA Biosystems) was used to perform 20 cycles of PCR amplification, with denaturing at 95°C for 30 s, annealing at 57°C for 30 s and extension at 72°C for 60 s. Post-amplification quality control was performed by on a Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). ..

Article Title: Biased transcription and selective degradation of small RNAs shape the pattern of DNA elimination in Tetrahymena
Article Snippet: .. The cDNA was amplified by subjecting 9.5 μL of the reaction to 17 cycles of PCR with KAPA Hifi Hot Start DNA polymerase (Kapa Biosystems) and Sol-fw and Sol-rv primers. .. The amplified cDNA library was fractionated in a 2% agarose gel; ∼100- to 200-base pair (bp) products were extracted, and single-read 50-base sequences were generated on a HiSeq 2000 platform (Illumina) to obtain ∼200 million reads.

Produced:

Article Title: Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes
Article Snippet: .. Typically, we employ 1.5 μL of the cellular DNA (1% of what was produced from a single well of a 96-well plate), which is usually around 15 ng of DNA, in a 10 μL amplification reaction using KAPA HiFi Hot Start DNA Polymerase (Kapa Biosystems, Wilmington, MA, USA). ..

Generated:

Article Title: Dominant-Negative Loss of Function Arises from a Second, More Frequent Variant Within the SAND Domain of Autoimmune Regulator (AIRE)
Article Snippet: .. Point mutations within AIRE plasmids were generated by using KAPA HiFi Hot start DNA polymerase (Kapa Biosystems, Wilmington, MA). .. The same mutagenesis strategy was used to introduce new epitope tags to make pCDNA3.1 hAIRE-HA and hAIRE-FLAG.

Amplification:

Article Title: High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution
Article Snippet: .. The KAPA HiFi Hot Start DNA Polymerase (KAPA Biosystems) was used to perform 20 cycles of PCR amplification, with denaturing at 95°C for 30 s, annealing at 57°C for 30 s and extension at 72°C for 60 s. Post-amplification quality control was performed by on a Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). ..

Article Title: Fusion to Flaviviral Leader Peptide Targets HIV-1 Reverse Transcriptase for Secretion and Reduces Its Enzymatic Activity and Ability to Induce Oxidative Stress but Has No Major Effects on Its Immunogenic Performance in DNA-Immunized Mice
Article Snippet: .. This fragment including 78 nucleotide b.p. was amplified with KAPA HiFi Hot Start DNA polymerase (Kapa Biosystems, USA) and Ld-NheI-F (5′-ATA-CGC-AAG-CTA-GCA-ATA-TGA-GAA-ACC-CTA-CAA-TG-3′) and Ld-BamHI-R (5′-TCT-AAC-AGG-ATC-CCG-CCC-CCA-CTC-CAA-GGG-3′) primers (Syntol, Russia), containing restriction sites NheI and BamHI , respectively. .. Resulting PCR product was cloned at the 5′-terminus of RT1.14 gene using NheI and BamHI restriction sites.

Article Title: Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes
Article Snippet: .. Typically, we employ 1.5 μL of the cellular DNA (1% of what was produced from a single well of a 96-well plate), which is usually around 15 ng of DNA, in a 10 μL amplification reaction using KAPA HiFi Hot Start DNA Polymerase (Kapa Biosystems, Wilmington, MA, USA). ..

Article Title: Biased transcription and selective degradation of small RNAs shape the pattern of DNA elimination in Tetrahymena
Article Snippet: .. The cDNA was amplified by subjecting 9.5 μL of the reaction to 17 cycles of PCR with KAPA Hifi Hot Start DNA polymerase (Kapa Biosystems) and Sol-fw and Sol-rv primers. .. The amplified cDNA library was fractionated in a 2% agarose gel; ∼100- to 200-base pair (bp) products were extracted, and single-read 50-base sequences were generated on a HiSeq 2000 platform (Illumina) to obtain ∼200 million reads.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 80
    Roche kapa hifi hot start dna polymerase
    Polymorphisms detected in the ITS-1 sequence of paper mulberry barkcloth textiles. Multiple alignment of 20 selected partial ITS sequences (50 bp) of textile samples. Image adapted from Bioedit 7.1.3.0. The reverse sequence (with primer ITS-C) is shown. The red arrow indicates the polymorphic nucleotide at the relative position 203 [ 21 ]. HM623778 corresponds to the ID of the B . papyrifera ITS region published in Genbank. M = double signal A/C at this position. Y = C or T; S = C or G; W = A or T. Bite Score (BS), Query Coverage (QC), E-values and identity percentage (Id) are indicated on the right. Extension names OT = sample amplified at the University of Otago using <t>KAPA</t> <t>HiFi</t> Hot Start <t>DNA</t> Polymerase; BM = sample amplified using GoTaq® G2 DNA Polymerase at the Faculty of Chemical and Pharmaceutical Sciences, University of Chile; MD = sample amplified using GoTaq® G2 Hot Start Flexi DNA Polymerase at the Faculty of Medicine, University of Chile, and P = sample amplified after extract purification.
    Kapa Hifi Hot Start Dna Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hifi hot start dna polymerase/product/Roche
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kapa hifi hot start dna polymerase - by Bioz Stars, 2020-08
    80/100 stars
      Buy from Supplier

    91
    Kapa Biosystems kapa hifi hot start dna polymerase
    Polymorphisms detected in the ITS-1 sequence of paper mulberry barkcloth textiles. Multiple alignment of 20 selected partial ITS sequences (50 bp) of textile samples. Image adapted from Bioedit 7.1.3.0. The reverse sequence (with primer ITS-C) is shown. The red arrow indicates the polymorphic nucleotide at the relative position 203 [ 21 ]. HM623778 corresponds to the ID of the B . papyrifera ITS region published in Genbank. M = double signal A/C at this position. Y = C or T; S = C or G; W = A or T. Bite Score (BS), Query Coverage (QC), E-values and identity percentage (Id) are indicated on the right. Extension names OT = sample amplified at the University of Otago using <t>KAPA</t> <t>HiFi</t> Hot Start <t>DNA</t> Polymerase; BM = sample amplified using GoTaq® G2 DNA Polymerase at the Faculty of Chemical and Pharmaceutical Sciences, University of Chile; MD = sample amplified using GoTaq® G2 Hot Start Flexi DNA Polymerase at the Faculty of Medicine, University of Chile, and P = sample amplified after extract purification.
    Kapa Hifi Hot Start Dna Polymerase, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hifi hot start dna polymerase/product/Kapa Biosystems
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    kapa hifi hot start dna polymerase - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    92
    Kapa Biosystems kapa hifi
    Polymorphisms detected in the ITS-1 sequence of paper mulberry barkcloth textiles. Multiple alignment of 20 selected partial ITS sequences (50 bp) of textile samples. Image adapted from Bioedit 7.1.3.0. The reverse sequence (with primer ITS-C) is shown. The red arrow indicates the polymorphic nucleotide at the relative position 203 [ 21 ]. HM623778 corresponds to the ID of the B . papyrifera ITS region published in Genbank. M = double signal A/C at this position. Y = C or T; S = C or G; W = A or T. Bite Score (BS), Query Coverage (QC), E-values and identity percentage (Id) are indicated on the right. Extension names OT = sample amplified at the University of Otago using <t>KAPA</t> <t>HiFi</t> Hot Start <t>DNA</t> Polymerase; BM = sample amplified using GoTaq® G2 DNA Polymerase at the Faculty of Chemical and Pharmaceutical Sciences, University of Chile; MD = sample amplified using GoTaq® G2 Hot Start Flexi DNA Polymerase at the Faculty of Medicine, University of Chile, and P = sample amplified after extract purification.
    Kapa Hifi, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 92/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa hifi/product/Kapa Biosystems
    Average 92 stars, based on 74 article reviews
    Price from $9.99 to $1999.99
    kapa hifi - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Polymorphisms detected in the ITS-1 sequence of paper mulberry barkcloth textiles. Multiple alignment of 20 selected partial ITS sequences (50 bp) of textile samples. Image adapted from Bioedit 7.1.3.0. The reverse sequence (with primer ITS-C) is shown. The red arrow indicates the polymorphic nucleotide at the relative position 203 [ 21 ]. HM623778 corresponds to the ID of the B . papyrifera ITS region published in Genbank. M = double signal A/C at this position. Y = C or T; S = C or G; W = A or T. Bite Score (BS), Query Coverage (QC), E-values and identity percentage (Id) are indicated on the right. Extension names OT = sample amplified at the University of Otago using KAPA HiFi Hot Start DNA Polymerase; BM = sample amplified using GoTaq® G2 DNA Polymerase at the Faculty of Chemical and Pharmaceutical Sciences, University of Chile; MD = sample amplified using GoTaq® G2 Hot Start Flexi DNA Polymerase at the Faculty of Medicine, University of Chile, and P = sample amplified after extract purification.

    Journal: PLoS ONE

    Article Title: A tale of textiles: Genetic characterization of historical paper mulberry barkcloth from Oceania

    doi: 10.1371/journal.pone.0233113

    Figure Lengend Snippet: Polymorphisms detected in the ITS-1 sequence of paper mulberry barkcloth textiles. Multiple alignment of 20 selected partial ITS sequences (50 bp) of textile samples. Image adapted from Bioedit 7.1.3.0. The reverse sequence (with primer ITS-C) is shown. The red arrow indicates the polymorphic nucleotide at the relative position 203 [ 21 ]. HM623778 corresponds to the ID of the B . papyrifera ITS region published in Genbank. M = double signal A/C at this position. Y = C or T; S = C or G; W = A or T. Bite Score (BS), Query Coverage (QC), E-values and identity percentage (Id) are indicated on the right. Extension names OT = sample amplified at the University of Otago using KAPA HiFi Hot Start DNA Polymerase; BM = sample amplified using GoTaq® G2 DNA Polymerase at the Faculty of Chemical and Pharmaceutical Sciences, University of Chile; MD = sample amplified using GoTaq® G2 Hot Start Flexi DNA Polymerase at the Faculty of Medicine, University of Chile, and P = sample amplified after extract purification.

    Article Snippet: Analyses at the University of Otago were performed with KAPA HiFi Hot Start DNA Polymerase (Kapa Biosystems, Wilmington, MS, USA), while GoTaq® G2 Flexi DNA Polymerase and GoTaq® G2 Hot Start Polymerase (Promega, Madison, WI, USA) were used at the facilities in Chile.

    Techniques: Sequencing, Amplification, Purification