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Kapa Biosystems kapa blood pcr kit
Genotyping of TPOX STR locus by RML-STR using <t>KAPA</t> <t>PCR</t> as target. TPOX sequences were amplified using 1.0 µl of whole blood in the KAPA Biosystems Blood PCR Kit. After PCR, the reaction was centrifuged and 4 µl of supernatant was used as target in standard RML-STR reaction. Reaction conditions and microarray detection method are as described in ‘Materials and Methods’ section.
Kapa Blood Pcr Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kapa blood pcr kit/product/Kapa Biosystems
Average 88 stars, based on 10 article reviews
Price from $9.99 to $1999.99
kapa blood pcr kit - by Bioz Stars, 2020-07
88/100 stars

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Article Title: Microarray-based STR genotyping using RecA-mediated ligation

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkq657

Genotyping of TPOX STR locus by RML-STR using KAPA PCR as target. TPOX sequences were amplified using 1.0 µl of whole blood in the KAPA Biosystems Blood PCR Kit. After PCR, the reaction was centrifuged and 4 µl of supernatant was used as target in standard RML-STR reaction. Reaction conditions and microarray detection method are as described in ‘Materials and Methods’ section.
Figure Legend Snippet: Genotyping of TPOX STR locus by RML-STR using KAPA PCR as target. TPOX sequences were amplified using 1.0 µl of whole blood in the KAPA Biosystems Blood PCR Kit. After PCR, the reaction was centrifuged and 4 µl of supernatant was used as target in standard RML-STR reaction. Reaction conditions and microarray detection method are as described in ‘Materials and Methods’ section.

Techniques Used: Polymerase Chain Reaction, Amplification, Microarray

Related Articles

Amplification:

Article Title: Apparent competition drives community-wide parasitism rates and changes in host abundance across ecosystem boundaries
Article Snippet: .. We amplified a ∼1 kb region of the 18S ribosomal RNA sequence, using the primer pair Nem_18S_F: 5′-CGC GAA TRG CTC ATT ACA ACA GC-3′ and Nem_18S_R: 5′-GGG CGG TAT CTG ATC GCC-3′ (ref. ), with KAPA Blood PCR Kit (Kapa Biosystems, USA). .. We used the following thermal cycling conditions: 94 °C for 3 min, 30 cycles of 94 °C (30 s), 55 °C (30 s), 72 °C (1 min), and a final extension of 72 °C for 2 min. We used the same purification, sequencing, and analysis methods for the nematode amplicons as for the Hymenoptera amplicons.

Article Title: Microarray-based STR genotyping using RecA-mediated ligation
Article Snippet: .. We have also shown successful genotyping using target amplified with the KAPA Blood PCR Kit (KAPA Biosystems, Woburn, MA). .. This enabled PCR using 1ul of whole blood and allowed deletion of the DNA extraction step from the standard protocol.

Sequencing:

Article Title: Apparent competition drives community-wide parasitism rates and changes in host abundance across ecosystem boundaries
Article Snippet: .. We amplified a ∼1 kb region of the 18S ribosomal RNA sequence, using the primer pair Nem_18S_F: 5′-CGC GAA TRG CTC ATT ACA ACA GC-3′ and Nem_18S_R: 5′-GGG CGG TAT CTG ATC GCC-3′ (ref. ), with KAPA Blood PCR Kit (Kapa Biosystems, USA). .. We used the following thermal cycling conditions: 94 °C for 3 min, 30 cycles of 94 °C (30 s), 55 °C (30 s), 72 °C (1 min), and a final extension of 72 °C for 2 min. We used the same purification, sequencing, and analysis methods for the nematode amplicons as for the Hymenoptera amplicons.

CTG Assay:

Article Title: Apparent competition drives community-wide parasitism rates and changes in host abundance across ecosystem boundaries
Article Snippet: .. We amplified a ∼1 kb region of the 18S ribosomal RNA sequence, using the primer pair Nem_18S_F: 5′-CGC GAA TRG CTC ATT ACA ACA GC-3′ and Nem_18S_R: 5′-GGG CGG TAT CTG ATC GCC-3′ (ref. ), with KAPA Blood PCR Kit (Kapa Biosystems, USA). .. We used the following thermal cycling conditions: 94 °C for 3 min, 30 cycles of 94 °C (30 s), 55 °C (30 s), 72 °C (1 min), and a final extension of 72 °C for 2 min. We used the same purification, sequencing, and analysis methods for the nematode amplicons as for the Hymenoptera amplicons.

Polymerase Chain Reaction:

Article Title: Apparent competition drives community-wide parasitism rates and changes in host abundance across ecosystem boundaries
Article Snippet: .. We amplified a ∼1 kb region of the 18S ribosomal RNA sequence, using the primer pair Nem_18S_F: 5′-CGC GAA TRG CTC ATT ACA ACA GC-3′ and Nem_18S_R: 5′-GGG CGG TAT CTG ATC GCC-3′ (ref. ), with KAPA Blood PCR Kit (Kapa Biosystems, USA). .. We used the following thermal cycling conditions: 94 °C for 3 min, 30 cycles of 94 °C (30 s), 55 °C (30 s), 72 °C (1 min), and a final extension of 72 °C for 2 min. We used the same purification, sequencing, and analysis methods for the nematode amplicons as for the Hymenoptera amplicons.

Article Title: Diversity of spotted fever group rickettsiae and their association with host ticks in Japan
Article Snippet: .. The PCR was carried out in a 25.0 μL reaction mixture containing 12.5 μL of 2 × KAPA blood PCR Kit (KAPA Biosystems, USA), 200 nM of each primer, 2.0 μL of DNA template, and sterile water. .. The reactions were performed at 95 °C for 5 min; followed by 45 cycles of 95 °C for 30 sec, 55 °C for 30 sec, and 72 °C for 40 sec; and 72 °C for 5 min. PCR products were electrophoresed at 100 V in a 1.2% agarose gel for 25 min. DNA from the R . japonica YH strain and sterile water were included in each PCR run as positive and negative controls, respectively.

Article Title: Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Detection of Ehrlichia ruminantium
Article Snippet: .. In order to reduce the effect of PCR inhibitors in the templates, the KAPA Blood PCR kit was used for the analysis of field samples. ..

Article Title: Microarray-based STR genotyping using RecA-mediated ligation
Article Snippet: .. We have also shown successful genotyping using target amplified with the KAPA Blood PCR Kit (KAPA Biosystems, Woburn, MA). .. This enabled PCR using 1ul of whole blood and allowed deletion of the DNA extraction step from the standard protocol.

Article Title: Cross-Institute Evaluations of Inhibitor-Resistant PCR Reagents for Direct Testing of Aerosol and Blood Samples Containing Biological Warfare Agent DNA
Article Snippet: .. These included three separate chemistries: Phire Hot Start DNA polymerase (New England BioLabs, Ipswich, MA), the Phusion Blood Direct PCR kit (New England BioLabs), and the KAPA Blood PCR kit (KAPA Biosystems, Woburn, MA). .. In addition, two buffers (Ampdirect buffer [Rockland Immunochemicals, Gilbertsville, PA] and STRboost buffer [Biomatrica, San Diego, CA]) were run with the Phire Hot Start polymerase (Phire).

Article Title: Apparent competition drives community-wide parasitism rates and changes in host abundance across ecosystem boundaries
Article Snippet: .. The extracted DNA was used as a template to amplify the target COI region using the primer pair HCO2198 (Folmer): 5′-TAA ACT TCA GGG TGA CCA AAA AAT CA-3′ and LCO1490 (Folmer): 5′-GGT CAA CAA ATC ATA AAG ATA TTG G-3′ (ref. ), with KAPA Blood PCR Kit (Kapa Biosystems, USA). .. We used 50 μl reactions and the following thermal cycling conditions: 94 °C for 3 min, 30 cycles of 94 °C (30 s), 52 °C (30 s), 72 °C (40 s), and a final extension of 72 °C for 2 min. 10 μl of the amplified product was resolved on a 1% agarose gel stained with SYBR Safe DNA Gel Stain (Life Technologies, USA) to check for correct amplification of the ∼650 bp product.

Article Title: Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Detection of Ehrlichia ruminantium
Article Snippet: .. PCR was performed with either the KAPA Blood PCR kit (Kapabiosystems, Boston, MA) or the AmpliTaq Gold PCR kit (Applied Biosystem). .. In order to reduce the effect of PCR inhibitors in the templates, the KAPA Blood PCR kit was used for the analysis of field samples.

Activated Clotting Time Assay:

Article Title: Apparent competition drives community-wide parasitism rates and changes in host abundance across ecosystem boundaries
Article Snippet: .. The extracted DNA was used as a template to amplify the target COI region using the primer pair HCO2198 (Folmer): 5′-TAA ACT TCA GGG TGA CCA AAA AAT CA-3′ and LCO1490 (Folmer): 5′-GGT CAA CAA ATC ATA AAG ATA TTG G-3′ (ref. ), with KAPA Blood PCR Kit (Kapa Biosystems, USA). .. We used 50 μl reactions and the following thermal cycling conditions: 94 °C for 3 min, 30 cycles of 94 °C (30 s), 52 °C (30 s), 72 °C (40 s), and a final extension of 72 °C for 2 min. 10 μl of the amplified product was resolved on a 1% agarose gel stained with SYBR Safe DNA Gel Stain (Life Technologies, USA) to check for correct amplification of the ∼650 bp product.

Cellular Antioxidant Activity Assay:

Article Title: Apparent competition drives community-wide parasitism rates and changes in host abundance across ecosystem boundaries
Article Snippet: .. The extracted DNA was used as a template to amplify the target COI region using the primer pair HCO2198 (Folmer): 5′-TAA ACT TCA GGG TGA CCA AAA AAT CA-3′ and LCO1490 (Folmer): 5′-GGT CAA CAA ATC ATA AAG ATA TTG G-3′ (ref. ), with KAPA Blood PCR Kit (Kapa Biosystems, USA). .. We used 50 μl reactions and the following thermal cycling conditions: 94 °C for 3 min, 30 cycles of 94 °C (30 s), 52 °C (30 s), 72 °C (40 s), and a final extension of 72 °C for 2 min. 10 μl of the amplified product was resolved on a 1% agarose gel stained with SYBR Safe DNA Gel Stain (Life Technologies, USA) to check for correct amplification of the ∼650 bp product.

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    Kapa Biosystems kapa blood pcr kit
    Genotyping of TPOX STR locus by RML-STR using <t>KAPA</t> <t>PCR</t> as target. TPOX sequences were amplified using 1.0 µl of whole blood in the KAPA Biosystems Blood PCR Kit. After PCR, the reaction was centrifuged and 4 µl of supernatant was used as target in standard RML-STR reaction. Reaction conditions and microarray detection method are as described in ‘Materials and Methods’ section.
    Kapa Blood Pcr Kit, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa blood pcr kit/product/Kapa Biosystems
    Average 88 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    kapa blood pcr kit - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    90
    Kapa Biosystems kapa sybr fast qpcr kit master mix 2x abi prism
    Genotyping of TPOX STR locus by RML-STR using <t>KAPA</t> <t>PCR</t> as target. TPOX sequences were amplified using 1.0 µl of whole blood in the KAPA Biosystems Blood PCR Kit. After PCR, the reaction was centrifuged and 4 µl of supernatant was used as target in standard RML-STR reaction. Reaction conditions and microarray detection method are as described in ‘Materials and Methods’ section.
    Kapa Sybr Fast Qpcr Kit Master Mix 2x Abi Prism, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kapa sybr fast qpcr kit master mix 2x abi prism/product/Kapa Biosystems
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    kapa sybr fast qpcr kit master mix 2x abi prism - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    Image Search Results


    Genotyping of TPOX STR locus by RML-STR using KAPA PCR as target. TPOX sequences were amplified using 1.0 µl of whole blood in the KAPA Biosystems Blood PCR Kit. After PCR, the reaction was centrifuged and 4 µl of supernatant was used as target in standard RML-STR reaction. Reaction conditions and microarray detection method are as described in ‘Materials and Methods’ section.

    Journal: Nucleic Acids Research

    Article Title: Microarray-based STR genotyping using RecA-mediated ligation

    doi: 10.1093/nar/gkq657

    Figure Lengend Snippet: Genotyping of TPOX STR locus by RML-STR using KAPA PCR as target. TPOX sequences were amplified using 1.0 µl of whole blood in the KAPA Biosystems Blood PCR Kit. After PCR, the reaction was centrifuged and 4 µl of supernatant was used as target in standard RML-STR reaction. Reaction conditions and microarray detection method are as described in ‘Materials and Methods’ section.

    Article Snippet: We have also shown successful genotyping using target amplified with the KAPA Blood PCR Kit (KAPA Biosystems, Woburn, MA).

    Techniques: Polymerase Chain Reaction, Amplification, Microarray