tetramethyl benzidine tmb substrate  (Echelon Biosciences)


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    Echelon Biosciences tetramethyl benzidine tmb substrate
    Tetramethyl Benzidine Tmb Substrate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tetramethyl benzidine tmb substrate/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
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    tetramethyl benzidine tmb substrate - by Bioz Stars, 2023-02
    94/100 stars

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    tetramethyl benzidine tmb substrate  (Echelon Biosciences)


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    Echelon Biosciences tetramethyl benzidine tmb substrate
    Tetramethyl Benzidine Tmb Substrate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tetramethyl benzidine tmb substrate/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tetramethyl benzidine tmb substrate - by Bioz Stars, 2023-02
    94/100 stars

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    k tmbp  (Echelon Biosciences)


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    Echelon Biosciences k tmbp
    K Tmbp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k tmbp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k tmbp - by Bioz Stars, 2023-02
    94/100 stars

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    k tmbp  (Echelon Biosciences)


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    Echelon Biosciences k tmbp
    K Tmbp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k tmbp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k tmbp - by Bioz Stars, 2023-02
    94/100 stars

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    k tmbp  (Echelon Biosciences)


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    Echelon Biosciences k tmbp
    K Tmbp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k tmbp/product/Echelon Biosciences
    Average 86 stars, based on 1 article reviews
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    k tmbp - by Bioz Stars, 2023-02
    86/100 stars

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    k tmbp  (Echelon Biosciences)


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    Echelon Biosciences k tmbp
    K Tmbp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k tmbp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
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    k tmbp - by Bioz Stars, 2023-02
    94/100 stars

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    k tmbp  (Echelon Biosciences)


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    Echelon Biosciences k tmbp
    A. PHLDA2 interferes with AKT to localize to the <t>cell</t> <t>membrane.</t> 293T cells were transfected with GFP-PH-AKT together with DsRed, DsRed-WT PHLDA2 or DsRed-mtPHLDA2 and subcellular localization was analyzed. Green and red arrows indicate AKT and PHLDA2 localized at the plasma membrane respectively. B. Binding of GST-PHLDA2 to PIP was assessed by protein-overlay assay. PIP strips were spotted with 15 different biologically active lipids at 100 pmol per spot. Bound proteins were detected with anti-GST antibody and detected with <t>K-TMBP</t> substrate. C. PHLDA2 inhibits PH-AKT binding to PIP2 and PIP3. Binding of GST-PH-AKT to immobilized PIP was assessed by protein-lipid overlay assay. Nitrocellulose membranes spotted with serially diluted PIP2 and PIP3 were incubated with the indicated proteins. While GST did not interfere with AKT binding to PIP, PHLDA2 significantly interfered. Bound AKT was detected with anti-AKT PH domain antibody.
    K Tmbp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k tmbp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k tmbp - by Bioz Stars, 2023-02
    94/100 stars

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    1) Product Images from "PHLDA2 is a key oncogene-induced negative feedback inhibitor of EGFR/ErbB2 signaling via interference with AKT signaling"

    Article Title: PHLDA2 is a key oncogene-induced negative feedback inhibitor of EGFR/ErbB2 signaling via interference with AKT signaling

    Journal: Oncotarget

    doi: 10.18632/oncotarget.3674

    A. PHLDA2 interferes with AKT to localize to the cell membrane. 293T cells were transfected with GFP-PH-AKT together with DsRed, DsRed-WT PHLDA2 or DsRed-mtPHLDA2 and subcellular localization was analyzed. Green and red arrows indicate AKT and PHLDA2 localized at the plasma membrane respectively. B. Binding of GST-PHLDA2 to PIP was assessed by protein-overlay assay. PIP strips were spotted with 15 different biologically active lipids at 100 pmol per spot. Bound proteins were detected with anti-GST antibody and detected with K-TMBP substrate. C. PHLDA2 inhibits PH-AKT binding to PIP2 and PIP3. Binding of GST-PH-AKT to immobilized PIP was assessed by protein-lipid overlay assay. Nitrocellulose membranes spotted with serially diluted PIP2 and PIP3 were incubated with the indicated proteins. While GST did not interfere with AKT binding to PIP, PHLDA2 significantly interfered. Bound AKT was detected with anti-AKT PH domain antibody.
    Figure Legend Snippet: A. PHLDA2 interferes with AKT to localize to the cell membrane. 293T cells were transfected with GFP-PH-AKT together with DsRed, DsRed-WT PHLDA2 or DsRed-mtPHLDA2 and subcellular localization was analyzed. Green and red arrows indicate AKT and PHLDA2 localized at the plasma membrane respectively. B. Binding of GST-PHLDA2 to PIP was assessed by protein-overlay assay. PIP strips were spotted with 15 different biologically active lipids at 100 pmol per spot. Bound proteins were detected with anti-GST antibody and detected with K-TMBP substrate. C. PHLDA2 inhibits PH-AKT binding to PIP2 and PIP3. Binding of GST-PH-AKT to immobilized PIP was assessed by protein-lipid overlay assay. Nitrocellulose membranes spotted with serially diluted PIP2 and PIP3 were incubated with the indicated proteins. While GST did not interfere with AKT binding to PIP, PHLDA2 significantly interfered. Bound AKT was detected with anti-AKT PH domain antibody.

    Techniques Used: Transfection, Binding Assay, Overlay Assay, Protein-lipid Overlay Assay (PLOA), Incubation

    k tmbp  (Echelon Biosciences)


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    Echelon Biosciences k tmbp
    A. PHLDA2 interferes with AKT to localize to the cell membrane. 293T cells were transfected with GFP-PH-AKT together with DsRed, DsRed-WT PHLDA2 or DsRed-mtPHLDA2 and subcellular localization was analyzed. Green and red arrows indicate AKT and PHLDA2 localized at the plasma membrane respectively. B. Binding of GST-PHLDA2 to PIP was assessed by protein-overlay assay. PIP strips were spotted with 15 different biologically active lipids at 100 pmol per spot. Bound proteins were detected with anti-GST antibody and detected with <t>K-TMBP</t> <t>substrate.</t> C. PHLDA2 inhibits PH-AKT binding to PIP2 and PIP3. Binding of GST-PH-AKT to immobilized PIP was assessed by protein-lipid overlay assay. Nitrocellulose membranes spotted with serially diluted PIP2 and PIP3 were incubated with the indicated proteins. While GST did not interfere with AKT binding to PIP, PHLDA2 significantly interfered. Bound AKT was detected with anti-AKT PH domain antibody.
    K Tmbp, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k tmbp/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k tmbp - by Bioz Stars, 2023-02
    94/100 stars

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    1) Product Images from "PHLDA2 is a key oncogene-induced negative feedback inhibitor of EGFR/ErbB2 signaling via interference with AKT signaling"

    Article Title: PHLDA2 is a key oncogene-induced negative feedback inhibitor of EGFR/ErbB2 signaling via interference with AKT signaling

    Journal: Oncotarget

    doi: 10.18632/oncotarget.3674

    A. PHLDA2 interferes with AKT to localize to the cell membrane. 293T cells were transfected with GFP-PH-AKT together with DsRed, DsRed-WT PHLDA2 or DsRed-mtPHLDA2 and subcellular localization was analyzed. Green and red arrows indicate AKT and PHLDA2 localized at the plasma membrane respectively. B. Binding of GST-PHLDA2 to PIP was assessed by protein-overlay assay. PIP strips were spotted with 15 different biologically active lipids at 100 pmol per spot. Bound proteins were detected with anti-GST antibody and detected with K-TMBP substrate. C. PHLDA2 inhibits PH-AKT binding to PIP2 and PIP3. Binding of GST-PH-AKT to immobilized PIP was assessed by protein-lipid overlay assay. Nitrocellulose membranes spotted with serially diluted PIP2 and PIP3 were incubated with the indicated proteins. While GST did not interfere with AKT binding to PIP, PHLDA2 significantly interfered. Bound AKT was detected with anti-AKT PH domain antibody.
    Figure Legend Snippet: A. PHLDA2 interferes with AKT to localize to the cell membrane. 293T cells were transfected with GFP-PH-AKT together with DsRed, DsRed-WT PHLDA2 or DsRed-mtPHLDA2 and subcellular localization was analyzed. Green and red arrows indicate AKT and PHLDA2 localized at the plasma membrane respectively. B. Binding of GST-PHLDA2 to PIP was assessed by protein-overlay assay. PIP strips were spotted with 15 different biologically active lipids at 100 pmol per spot. Bound proteins were detected with anti-GST antibody and detected with K-TMBP substrate. C. PHLDA2 inhibits PH-AKT binding to PIP2 and PIP3. Binding of GST-PH-AKT to immobilized PIP was assessed by protein-lipid overlay assay. Nitrocellulose membranes spotted with serially diluted PIP2 and PIP3 were incubated with the indicated proteins. While GST did not interfere with AKT binding to PIP, PHLDA2 significantly interfered. Bound AKT was detected with anti-AKT PH domain antibody.

    Techniques Used: Transfection, Binding Assay, Overlay Assay, Protein-lipid Overlay Assay (PLOA), Incubation

    k tmbp substrate  (Echelon Biosciences)


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    Echelon Biosciences k tmbp substrate
    K Tmbp Substrate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k tmbp substrate/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k tmbp substrate - by Bioz Stars, 2023-02
    94/100 stars

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    k tmbp substrate  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
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    Echelon Biosciences k tmbp substrate
    K Tmbp Substrate, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k tmbp substrate/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k tmbp substrate - by Bioz Stars, 2023-02
    94/100 stars

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