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hyaluronidase activity elisa  (Echelon Biosciences)


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    Echelon Biosciences hyaluronidase activity elisa
    Hyaluronidase Activity Elisa, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hyaluronidase activity elisa/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    hyaluronidase activity elisa - by Bioz Stars, 2025-04
    94/100 stars

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    HYAL1 knockdown protected endothelial glycocalyx in LPS-induced AKI mice. (A) Western blotting for syndecan-1 and heparanase-1 protein levels in murine kidneys of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (B) ELISA for <t>hyaluronidase</t> activity in murine serum of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (C, D) ELISA for hyaluronic acid level in murine serum and urine of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (E) Immunofluorescence staining for hyaluronic acid level in murine renal endothelial glycocalyx of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups (scale bar = 20 μm). (F) The quantification of the hyaluronic acid fluorescence intensity. (G, H) IHC staining for hyaluronic acid level within tubulointerstitial (scale bar = 50 μm). N = 10 mice each group. ** p < .01, *** p < .001 vs. Sham + LV-NC group; # p < .05, ## p < .01 vs. LPS + LV-NC group.
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    HYAL1 knockdown protected endothelial glycocalyx in LPS-induced AKI mice. (A) Western blotting for syndecan-1 and heparanase-1 protein levels in murine kidneys of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (B) ELISA for hyaluronidase activity in murine serum of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (C, D) ELISA for hyaluronic acid level in murine serum and urine of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (E) Immunofluorescence staining for hyaluronic acid level in murine renal endothelial glycocalyx of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups (scale bar = 20 μm). (F) The quantification of the hyaluronic acid fluorescence intensity. (G, H) IHC staining for hyaluronic acid level within tubulointerstitial (scale bar = 50 μm). N = 10 mice each group. ** p < .01, *** p < .001 vs. Sham + LV-NC group; # p < .05, ## p < .01 vs. LPS + LV-NC group.

    Journal: Renal Failure

    Article Title: HYAL1 deficiency attenuates lipopolysaccharide-triggered renal injury and endothelial glycocalyx breakdown in septic AKI in mice

    doi: 10.1080/0886022X.2023.2188966

    Figure Lengend Snippet: HYAL1 knockdown protected endothelial glycocalyx in LPS-induced AKI mice. (A) Western blotting for syndecan-1 and heparanase-1 protein levels in murine kidneys of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (B) ELISA for hyaluronidase activity in murine serum of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (C, D) ELISA for hyaluronic acid level in murine serum and urine of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (E) Immunofluorescence staining for hyaluronic acid level in murine renal endothelial glycocalyx of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups (scale bar = 20 μm). (F) The quantification of the hyaluronic acid fluorescence intensity. (G, H) IHC staining for hyaluronic acid level within tubulointerstitial (scale bar = 50 μm). N = 10 mice each group. ** p < .01, *** p < .001 vs. Sham + LV-NC group; # p < .05, ## p < .01 vs. LPS + LV-NC group.

    Article Snippet: Hyaluronidase activity was assessed by an ELISA kit (Echelon Biosciences, USA).

    Techniques: Knockdown, Western Blot, Enzyme-linked Immunosorbent Assay, Activity Assay, Immunofluorescence, Staining, Fluorescence, Immunohistochemistry

    HYAL1 knockdown protected endothelial glycocalyx in LPS-induced AKI mice. (A) Western blotting for syndecan-1 and heparanase-1 protein levels in murine kidneys of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (B) ELISA for hyaluronidase activity in murine serum of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (C, D) ELISA for hyaluronic acid level in murine serum and urine of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (E) Immunofluorescence staining for hyaluronic acid level in murine renal endothelial glycocalyx of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups (scale bar = 20 μm). (F) The quantification of the hyaluronic acid fluorescence intensity. (G, H) IHC staining for hyaluronic acid level within tubulointerstitial (scale bar = 50 μm). N = 10 mice each group. ** p < .01, *** p < .001 vs. Sham + LV-NC group; # p < .05, ## p < .01 vs. LPS + LV-NC group.

    Journal: Renal Failure

    Article Title: HYAL1 deficiency attenuates lipopolysaccharide-triggered renal injury and endothelial glycocalyx breakdown in septic AKI in mice

    doi: 10.1080/0886022X.2023.2188966

    Figure Lengend Snippet: HYAL1 knockdown protected endothelial glycocalyx in LPS-induced AKI mice. (A) Western blotting for syndecan-1 and heparanase-1 protein levels in murine kidneys of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (B) ELISA for hyaluronidase activity in murine serum of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (C, D) ELISA for hyaluronic acid level in murine serum and urine of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups. (E) Immunofluorescence staining for hyaluronic acid level in murine renal endothelial glycocalyx of Sham + LV-NC, Sham + LV-sh-HYAL1, LPS + LV-NC and LPS + LV-sh-HYAL1 groups (scale bar = 20 μm). (F) The quantification of the hyaluronic acid fluorescence intensity. (G, H) IHC staining for hyaluronic acid level within tubulointerstitial (scale bar = 50 μm). N = 10 mice each group. ** p < .01, *** p < .001 vs. Sham + LV-NC group; # p < .05, ## p < .01 vs. LPS + LV-NC group.

    Article Snippet: Hyaluronidase activity was assessed by an ELISA kit (Echelon Biosciences, USA).

    Techniques: Knockdown, Western Blot, Enzyme-linked Immunosorbent Assay, Activity Assay, Immunofluorescence, Staining, Fluorescence, Immunohistochemistry