Journal: Cancer Science

Article Title: Autotaxin in ascites promotes peritoneal dissemination in pancreatic cancer

doi: 10.1111/cas.14689

Figure Lengend Snippet: Assessment of peritoneal dissemination in an in vivo peritoneal dissemination model. A, Measurement of autotaxin (ATX) activity in murine serum. PF‐8380, solubilized in 2:1 dimethyl sulfoxide (DMSO)‐saline, was injected intraperitoneally at the dose of 10 mg/kg body weight twice a day for 3 d. Using the ATX Activity Kit, ATX activity in the serum collected 8 h after the last PF‐8380 injection or DMSO‐saline injection in the control group was measured. Data represent the means of the control group (n = 8) or PF‐8380 group (n = 3). Bars, standard error; * P < .01. B, Representative images of nude mice with peritoneal dissemination on day 23 after AsPC1/luc cell implantation. Upper, bioluminescence imaging; middle, image under white light; lower, magnified images of peritoneal disseminated tumors. Red arrows indicate the peritoneal disseminated tumors. C, Quantification of bioluminescence imaging. Data represent the means of the control group (n = 11) and the PF‐8380 group (n = 13). Bars, standard error; * P < .001. D, Measurement of the amount of ascites. Data represent the means of the control group (n = 11) and PF‐8380 group (n = 13). Bars, standard error; * P < .001

Article Snippet: Using the collected samples, the ATX concentration and activity were measured with an ATX Sandwich ELISA Kit (Echelon Biosciences Inc) and an ATX Activity Kit (Echelon Biosciences Inc), according to the manufacturer's instructions.

Techniques: In Vivo, Activity Assay, Injection, Imaging

Journal: Journal of Lipid Research

Article Title: Deficiency of alkaline SMase enhances dextran sulfate sodium-induced colitis in mice with upregulation of autotaxin

doi: 10.1194/jlr.M084285

Figure Lengend Snippet: Autotaxin expression in both small intestine and colon. The animals were treated with DSS for 2 and 4 days and the expression of autotaxin in small intestine and colon treatment was studied by IHC. A: The autotaxin expression in the small intestine. As indicated by the arrows, the expression was weak on day 0 and increased thereafter. The increase is more obvious in KO than in WT mice. The expression is mainly in the lamina propria of villi and vessels (arrowed). B: The autotaxin expression in the colon. Essentially no positive stain was identified before DSS treatment (day 0) in both WT and KO groups, but was obviously visible on day 2 and day 4, with that in KO mice being more intensive than in the WT group. The expression was also by endothelial and immune cells (arrowed) in the lamina propria region of the crypt. C: The expression of autotaxin in the endothelium of blood vessels on day 4 in WT and KO mice.

Article Snippet: The sandwich ELISA kit for autotaxin quantification was purchased from Echelon Biosciences Inc. (Salt Lake City, UT).

Techniques: Expressing, Staining

Journal: Journal of Lipid Research

Article Title: Deficiency of alkaline SMase enhances dextran sulfate sodium-induced colitis in mice with upregulation of autotaxin

doi: 10.1194/jlr.M084285

Figure Lengend Snippet: Western blot for autotaxin (Atx) in the small intestine and colon. The animals were treated with DSS for 4 days. Western blot for autotaxin was performed in the homogenates of both colonic and small intestinal mucosa. The right panels show the representative results of one experiment. The densities of the bands from all mice were determined related to that in the WT group. N = 3 for both WT and KO mice. *P < 0.05 compared with WT mice.

Article Snippet: The sandwich ELISA kit for autotaxin quantification was purchased from Echelon Biosciences Inc. (Salt Lake City, UT).

Techniques: Western Blot

Journal: Journal of Lipid Research

Article Title: Deficiency of alkaline SMase enhances dextran sulfate sodium-induced colitis in mice with upregulation of autotaxin

doi: 10.1194/jlr.M084285

Figure Lengend Snippet: ELISA for autotaxin (Atx) in the small intestine (A) and colon (B). The WT and KO mice were treated with DSS for 4 days. The autotaxin levels were assayed in the homogenates of both colonic and small intestinal mucosa. N = 4 for both WT and KO mice. *P < 0.05, **P < 0.01 compared with the WT mice.

Article Snippet: The sandwich ELISA kit for autotaxin quantification was purchased from Echelon Biosciences Inc. (Salt Lake City, UT).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Serum fatty acid-binding protein 4 (FABP4) concentration is associated with insulin resistance in peripheral tissues, A clinical study

doi: 10.1371/journal.pone.0179737

Figure Lengend Snippet: Baseline characteristics.

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) kit was used for measuring the following: A-FABP (human Adipocity FABP ELISA kit, RD191036200R, BioVendor R & D, Bruno, Czech Republic), irisin (Phoenix Pharmaceuticals, EK-067-29,Funakoshi, Tokyo, Japan), IGF-1 (IGF-1 immunoradiometric assay [IRMA], Daiichi, BPKB195, Tokyo, Japan), plasma adiponectin (human adiponectin ELISA kit, 410614, Otsuka, Tokyo, Japan), ATX (Autotaxin Sandwich ELISA, K-5600, Echelon Biosciences Inc. Salt Lake City, UT, USA), and plasma IL-6 (human IL-6 Quantikine ELISA, D6050, R & D Systems, Inc., Minneapolis, MN, USA).

Techniques:

Journal: PLoS ONE

Article Title: Serum fatty acid-binding protein 4 (FABP4) concentration is associated with insulin resistance in peripheral tissues, A clinical study

doi: 10.1371/journal.pone.0179737

Figure Lengend Snippet: (A) Fatty acid binding protein-4 (FABP4), Type 2 diabetes mellitus, (B) FABP4, non- diabetes mellitus, (C) Adiponectin, Type 2 diabetes mellitus (D) Adiponectin, non- diabetes mellitus, (E) Autotaxin (ATX), Type 2 diabetes mellitus, (F) ATX, non-DM, (G) Irisin, T2 diabetes mellitus, (H) Irisin, non- diabetes mellitus, (I) Interleukin (IL)-6, Type 2 diabetes mellitus, (J) IL-6, non- diabetes mellitus, (K) Insulin like growth factor (IGF)-1, Type 2 diabetes mellitus, (L) IGF-1, non- diabetes mellitus.

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) kit was used for measuring the following: A-FABP (human Adipocity FABP ELISA kit, RD191036200R, BioVendor R & D, Bruno, Czech Republic), irisin (Phoenix Pharmaceuticals, EK-067-29,Funakoshi, Tokyo, Japan), IGF-1 (IGF-1 immunoradiometric assay [IRMA], Daiichi, BPKB195, Tokyo, Japan), plasma adiponectin (human adiponectin ELISA kit, 410614, Otsuka, Tokyo, Japan), ATX (Autotaxin Sandwich ELISA, K-5600, Echelon Biosciences Inc. Salt Lake City, UT, USA), and plasma IL-6 (human IL-6 Quantikine ELISA, D6050, R & D Systems, Inc., Minneapolis, MN, USA).

Techniques: Binding Assay

Journal: PLoS ONE

Article Title: Serum fatty acid-binding protein 4 (FABP4) concentration is associated with insulin resistance in peripheral tissues, A clinical study

doi: 10.1371/journal.pone.0179737

Figure Lengend Snippet: Correlation with GDR.

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) kit was used for measuring the following: A-FABP (human Adipocity FABP ELISA kit, RD191036200R, BioVendor R & D, Bruno, Czech Republic), irisin (Phoenix Pharmaceuticals, EK-067-29,Funakoshi, Tokyo, Japan), IGF-1 (IGF-1 immunoradiometric assay [IRMA], Daiichi, BPKB195, Tokyo, Japan), plasma adiponectin (human adiponectin ELISA kit, 410614, Otsuka, Tokyo, Japan), ATX (Autotaxin Sandwich ELISA, K-5600, Echelon Biosciences Inc. Salt Lake City, UT, USA), and plasma IL-6 (human IL-6 Quantikine ELISA, D6050, R & D Systems, Inc., Minneapolis, MN, USA).

Techniques:

Journal: PLoS ONE

Article Title: Serum fatty acid-binding protein 4 (FABP4) concentration is associated with insulin resistance in peripheral tissues, A clinical study

doi: 10.1371/journal.pone.0179737

Figure Lengend Snippet: Body composition and factors providing insulin resistance.

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) kit was used for measuring the following: A-FABP (human Adipocity FABP ELISA kit, RD191036200R, BioVendor R & D, Bruno, Czech Republic), irisin (Phoenix Pharmaceuticals, EK-067-29,Funakoshi, Tokyo, Japan), IGF-1 (IGF-1 immunoradiometric assay [IRMA], Daiichi, BPKB195, Tokyo, Japan), plasma adiponectin (human adiponectin ELISA kit, 410614, Otsuka, Tokyo, Japan), ATX (Autotaxin Sandwich ELISA, K-5600, Echelon Biosciences Inc. Salt Lake City, UT, USA), and plasma IL-6 (human IL-6 Quantikine ELISA, D6050, R & D Systems, Inc., Minneapolis, MN, USA).

Techniques:

Journal: PLoS ONE

Article Title: Serum fatty acid-binding protein 4 (FABP4) concentration is associated with insulin resistance in peripheral tissues, A clinical study

doi: 10.1371/journal.pone.0179737

Figure Lengend Snippet: Baseline characteristics.

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) kit was used for measuring the following: A-FABP (human Adipocity FABP ELISA kit, RD191036200R, BioVendor R & D, Bruno, Czech Republic), irisin (Phoenix Pharmaceuticals, EK-067-29,Funakoshi, Tokyo, Japan), IGF-1 (IGF-1 immunoradiometric assay [IRMA], Daiichi, BPKB195, Tokyo, Japan), plasma adiponectin (human adiponectin ELISA kit, 410614, Otsuka, Tokyo, Japan), ATX (Autotaxin Sandwich ELISA, K-5600, Echelon Biosciences Inc. Salt Lake City, UT, USA), and plasma IL-6 (human IL-6 Quantikine ELISA, D6050, R & D Systems, Inc., Minneapolis, MN, USA).

Techniques:

Journal: PLoS ONE

Article Title: Serum fatty acid-binding protein 4 (FABP4) concentration is associated with insulin resistance in peripheral tissues, A clinical study

doi: 10.1371/journal.pone.0179737

Figure Lengend Snippet: (A) Fatty acid binding protein-4 (FABP4), Type 2 diabetes mellitus, (B) FABP4, non- diabetes mellitus, (C) Adiponectin, Type 2 diabetes mellitus (D) Adiponectin, non- diabetes mellitus, (E) Autotaxin (ATX), Type 2 diabetes mellitus, (F) ATX, non-DM, (G) Irisin, T2 diabetes mellitus, (H) Irisin, non- diabetes mellitus, (I) Interleukin (IL)-6, Type 2 diabetes mellitus, (J) IL-6, non- diabetes mellitus, (K) Insulin like growth factor (IGF)-1, Type 2 diabetes mellitus, (L) IGF-1, non- diabetes mellitus.

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) kit was used for measuring the following: A-FABP (human Adipocity FABP ELISA kit, RD191036200R, BioVendor R & D, Bruno, Czech Republic), irisin (Phoenix Pharmaceuticals, EK-067-29,Funakoshi, Tokyo, Japan), IGF-1 (IGF-1 immunoradiometric assay [IRMA], Daiichi, BPKB195, Tokyo, Japan), plasma adiponectin (human adiponectin ELISA kit, 410614, Otsuka, Tokyo, Japan), ATX (Autotaxin Sandwich ELISA, K-5600, Echelon Biosciences Inc. Salt Lake City, UT, USA), and plasma IL-6 (human IL-6 Quantikine ELISA, D6050, R & D Systems, Inc., Minneapolis, MN, USA).

Techniques: Binding Assay

Journal: PLoS ONE

Article Title: Serum fatty acid-binding protein 4 (FABP4) concentration is associated with insulin resistance in peripheral tissues, A clinical study

doi: 10.1371/journal.pone.0179737

Figure Lengend Snippet: Correlation with GDR.

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) kit was used for measuring the following: A-FABP (human Adipocity FABP ELISA kit, RD191036200R, BioVendor R & D, Bruno, Czech Republic), irisin (Phoenix Pharmaceuticals, EK-067-29,Funakoshi, Tokyo, Japan), IGF-1 (IGF-1 immunoradiometric assay [IRMA], Daiichi, BPKB195, Tokyo, Japan), plasma adiponectin (human adiponectin ELISA kit, 410614, Otsuka, Tokyo, Japan), ATX (Autotaxin Sandwich ELISA, K-5600, Echelon Biosciences Inc. Salt Lake City, UT, USA), and plasma IL-6 (human IL-6 Quantikine ELISA, D6050, R & D Systems, Inc., Minneapolis, MN, USA).

Techniques:

Journal: PLoS ONE

Article Title: Serum fatty acid-binding protein 4 (FABP4) concentration is associated with insulin resistance in peripheral tissues, A clinical study

doi: 10.1371/journal.pone.0179737

Figure Lengend Snippet: Body composition and factors providing insulin resistance.

Article Snippet: An enzyme-linked immunosorbent assay (ELISA) kit was used for measuring the following: A-FABP (human Adipocity FABP ELISA kit, RD191036200R, BioVendor R & D, Bruno, Czech Republic), irisin (Phoenix Pharmaceuticals, EK-067-29,Funakoshi, Tokyo, Japan), IGF-1 (IGF-1 immunoradiometric assay [IRMA], Daiichi, BPKB195, Tokyo, Japan), plasma adiponectin (human adiponectin ELISA kit, 410614, Otsuka, Tokyo, Japan), ATX (Autotaxin Sandwich ELISA, K-5600, Echelon Biosciences Inc. Salt Lake City, UT, USA), and plasma IL-6 (human IL-6 Quantikine ELISA, D6050, R & D Systems, Inc., Minneapolis, MN, USA).

Techniques: