Journal: PLoS ONE
Article Title: Autotaxin Signaling Governs Phenotypic Heterogeneity in Visceral and Parietal Mesothelia
doi: 10.1371/journal.pone.0069712
Figure Lengend Snippet: Immunofluorescence was used to detect autotaxin (ATX) expression in visceral and parietal tissues from Wt1 cre/+ ; R26R eYFP/+ reporter mice. Cells that express Wt1, a mesothelial marker, will express enhanced yellow fluorescent protein (eYFP) that can be detected with a green fluorescent protein (GFP) antibody (A, D, G). Robust autotaxin expression is evident in visceral mesothelia (A–F). In contrast, the parietal mesothelium that lines the body wall expresses the mesothelial marker (G), but is devoid of autotaxin expression (H). Nuclei are marked with DAPI. Quantitative real-time RT-PCR was used to determine autotaxin transcript levels in visceral (omental) mesothelium, parietal mesothelium, heart, and liver (J). The autotaxin expression in visceral cells was set to 1 and fold changes were calculated for each subsequent tissue type. The asterisk represents a statistically significant difference when compared to the expression level in visceral cells (p<0.05, n = 4). Error bars were calculated using standard error of the mean.
Article Snippet: Visceral mesothelial, parietal mesothelial, MSTO, and ROB cells were cultured in serum free medium for 48 h. Conditioned media from these cells was collected and used in a commercially available autotaxin activity assay (Echelon, K-4100).
Techniques: Immunofluorescence, Expressing, Marker, Quantitative RT-PCR