autotaxin activity  (Echelon Biosciences)


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    Echelon Biosciences autotaxin activity
    Elevated <t>autotaxin</t> (ATX) activity following ischemia and reperfusion (I/R). A , Triphenyl tetrazolium chloride staining of mouse brain slices and respective infarct volume (percentage of the hemisphere) measured in sham and I/R mice. B , Mouse brain cryosections stained for microglia (Iba1) in sham and I/R mice ipsilateral cortex. Bar=200 µm. C , ATX mRNA expression was measured in ipsilateral cortex brain tissue lysate from sham and I/R mice. D , Enzymatic activity test for ATX measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU) in sham and I/R mouse brain slices. E , Lysophosphatidylcholine subspecies in plasma from sham and I/R mice, measured with liquid chromatography–mass spectrometry, quantified relative to 18:1 LPC. All values are mean±SD compared with the sham group using Mann‐Whitney U test. DAPI indicates 4′,6‐diamidino‐2‐phenylindole. NI, near‐infrared.
    Autotaxin Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/autotaxin activity/product/Echelon Biosciences
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    autotaxin activity - by Bioz Stars, 2022-09
    93/100 stars

    Images

    1) Product Images from "Disrupted Blood‐Brain Barrier and Mitochondrial Impairment by Autotaxin–Lysophosphatidic Acid Axis in Postischemic Stroke"

    Article Title: Disrupted Blood‐Brain Barrier and Mitochondrial Impairment by Autotaxin–Lysophosphatidic Acid Axis in Postischemic Stroke

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    doi: 10.1161/JAHA.121.021511

    Elevated autotaxin (ATX) activity following ischemia and reperfusion (I/R). A , Triphenyl tetrazolium chloride staining of mouse brain slices and respective infarct volume (percentage of the hemisphere) measured in sham and I/R mice. B , Mouse brain cryosections stained for microglia (Iba1) in sham and I/R mice ipsilateral cortex. Bar=200 µm. C , ATX mRNA expression was measured in ipsilateral cortex brain tissue lysate from sham and I/R mice. D , Enzymatic activity test for ATX measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU) in sham and I/R mouse brain slices. E , Lysophosphatidylcholine subspecies in plasma from sham and I/R mice, measured with liquid chromatography–mass spectrometry, quantified relative to 18:1 LPC. All values are mean±SD compared with the sham group using Mann‐Whitney U test. DAPI indicates 4′,6‐diamidino‐2‐phenylindole. NI, near‐infrared.
    Figure Legend Snippet: Elevated autotaxin (ATX) activity following ischemia and reperfusion (I/R). A , Triphenyl tetrazolium chloride staining of mouse brain slices and respective infarct volume (percentage of the hemisphere) measured in sham and I/R mice. B , Mouse brain cryosections stained for microglia (Iba1) in sham and I/R mice ipsilateral cortex. Bar=200 µm. C , ATX mRNA expression was measured in ipsilateral cortex brain tissue lysate from sham and I/R mice. D , Enzymatic activity test for ATX measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU) in sham and I/R mouse brain slices. E , Lysophosphatidylcholine subspecies in plasma from sham and I/R mice, measured with liquid chromatography–mass spectrometry, quantified relative to 18:1 LPC. All values are mean±SD compared with the sham group using Mann‐Whitney U test. DAPI indicates 4′,6‐diamidino‐2‐phenylindole. NI, near‐infrared.

    Techniques Used: Activity Assay, Staining, Mouse Assay, Expressing, Fluorescence, Liquid Chromatography, Mass Spectrometry, MANN-WHITNEY

    Autotaxin (ATX) inhibitors in middle cerebral artery occlusion decrease ATX activity and lysophosphatidic acid (LPA). A , Enzymatic activity test for ATX, measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU), in sham, ischemia and reperfusion (I/R), HA130, and PF8380 mouse brain slices. B , Lysophosphatidylcholine (LPC) subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with high‐performance liquid chromatography–tandem mass spectrometry (liquid chromatography–mass spectrometry [LC‐MS]) quantified relative to 18:1 LPC. C , LPA subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with LC‐MS, quantified relative to 18:1 LPA. D and E , Graph represents oxygen consumption rate (OCR) (pmol/min per µg protein) measurements in isolated mitochondria from sham, I/R, HA130, PF8380, or BrP‐LPA mouse brain tissue at baseline and after sequential addition of oligomycin, carbonyl cyanide p‐trifluoro‐methoxyphenyl hydrazone (FCCP), and antimycin A+rotenone. F , Spare respiratory capacity, ATP turnover, and maximum respiration values analyzed in sham, I/R, HA130, PF8380, or BrP‐LPA mice. All values are mean±SD. * P
    Figure Legend Snippet: Autotaxin (ATX) inhibitors in middle cerebral artery occlusion decrease ATX activity and lysophosphatidic acid (LPA). A , Enzymatic activity test for ATX, measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU), in sham, ischemia and reperfusion (I/R), HA130, and PF8380 mouse brain slices. B , Lysophosphatidylcholine (LPC) subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with high‐performance liquid chromatography–tandem mass spectrometry (liquid chromatography–mass spectrometry [LC‐MS]) quantified relative to 18:1 LPC. C , LPA subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with LC‐MS, quantified relative to 18:1 LPA. D and E , Graph represents oxygen consumption rate (OCR) (pmol/min per µg protein) measurements in isolated mitochondria from sham, I/R, HA130, PF8380, or BrP‐LPA mouse brain tissue at baseline and after sequential addition of oligomycin, carbonyl cyanide p‐trifluoro‐methoxyphenyl hydrazone (FCCP), and antimycin A+rotenone. F , Spare respiratory capacity, ATP turnover, and maximum respiration values analyzed in sham, I/R, HA130, PF8380, or BrP‐LPA mice. All values are mean±SD. * P

    Techniques Used: Activity Assay, Fluorescence, Mouse Assay, High Performance Liquid Chromatography, Mass Spectrometry, Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy, Isolation

    2) Product Images from "Disrupted Blood‐Brain Barrier and Mitochondrial Impairment by Autotaxin–Lysophosphatidic Acid Axis in Postischemic Stroke"

    Article Title: Disrupted Blood‐Brain Barrier and Mitochondrial Impairment by Autotaxin–Lysophosphatidic Acid Axis in Postischemic Stroke

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    doi: 10.1161/JAHA.121.021511

    Elevated autotaxin (ATX) activity following ischemia and reperfusion (I/R). A , Triphenyl tetrazolium chloride staining of mouse brain slices and respective infarct volume (percentage of the hemisphere) measured in sham and I/R mice. B , Mouse brain cryosections stained for microglia (Iba1) in sham and I/R mice ipsilateral cortex. Bar=200 µm. C , ATX mRNA expression was measured in ipsilateral cortex brain tissue lysate from sham and I/R mice. D , Enzymatic activity test for ATX measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU) in sham and I/R mouse brain slices. E , Lysophosphatidylcholine subspecies in plasma from sham and I/R mice, measured with liquid chromatography–mass spectrometry, quantified relative to 18:1 LPC. All values are mean±SD compared with the sham group using Mann‐Whitney U test. DAPI indicates 4′,6‐diamidino‐2‐phenylindole. NI, near‐infrared.
    Figure Legend Snippet: Elevated autotaxin (ATX) activity following ischemia and reperfusion (I/R). A , Triphenyl tetrazolium chloride staining of mouse brain slices and respective infarct volume (percentage of the hemisphere) measured in sham and I/R mice. B , Mouse brain cryosections stained for microglia (Iba1) in sham and I/R mice ipsilateral cortex. Bar=200 µm. C , ATX mRNA expression was measured in ipsilateral cortex brain tissue lysate from sham and I/R mice. D , Enzymatic activity test for ATX measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU) in sham and I/R mouse brain slices. E , Lysophosphatidylcholine subspecies in plasma from sham and I/R mice, measured with liquid chromatography–mass spectrometry, quantified relative to 18:1 LPC. All values are mean±SD compared with the sham group using Mann‐Whitney U test. DAPI indicates 4′,6‐diamidino‐2‐phenylindole. NI, near‐infrared.

    Techniques Used: Activity Assay, Staining, Mouse Assay, Expressing, Fluorescence, Liquid Chromatography, Mass Spectrometry, MANN-WHITNEY

    Autotaxin (ATX) inhibitors in middle cerebral artery occlusion decrease ATX activity and lysophosphatidic acid (LPA). A , Enzymatic activity test for ATX, measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU), in sham, ischemia and reperfusion (I/R), HA130, and PF8380 mouse brain slices. B , Lysophosphatidylcholine (LPC) subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with high‐performance liquid chromatography–tandem mass spectrometry (liquid chromatography–mass spectrometry [LC‐MS]) quantified relative to 18:1 LPC. C , LPA subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with LC‐MS, quantified relative to 18:1 LPA. D and E , Graph represents oxygen consumption rate (OCR) (pmol/min per µg protein) measurements in isolated mitochondria from sham, I/R, HA130, PF8380, or BrP‐LPA mouse brain tissue at baseline and after sequential addition of oligomycin, carbonyl cyanide p‐trifluoro‐methoxyphenyl hydrazone (FCCP), and antimycin A+rotenone. F , Spare respiratory capacity, ATP turnover, and maximum respiration values analyzed in sham, I/R, HA130, PF8380, or BrP‐LPA mice. All values are mean±SD. * P
    Figure Legend Snippet: Autotaxin (ATX) inhibitors in middle cerebral artery occlusion decrease ATX activity and lysophosphatidic acid (LPA). A , Enzymatic activity test for ATX, measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU), in sham, ischemia and reperfusion (I/R), HA130, and PF8380 mouse brain slices. B , Lysophosphatidylcholine (LPC) subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with high‐performance liquid chromatography–tandem mass spectrometry (liquid chromatography–mass spectrometry [LC‐MS]) quantified relative to 18:1 LPC. C , LPA subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with LC‐MS, quantified relative to 18:1 LPA. D and E , Graph represents oxygen consumption rate (OCR) (pmol/min per µg protein) measurements in isolated mitochondria from sham, I/R, HA130, PF8380, or BrP‐LPA mouse brain tissue at baseline and after sequential addition of oligomycin, carbonyl cyanide p‐trifluoro‐methoxyphenyl hydrazone (FCCP), and antimycin A+rotenone. F , Spare respiratory capacity, ATP turnover, and maximum respiration values analyzed in sham, I/R, HA130, PF8380, or BrP‐LPA mice. All values are mean±SD. * P

    Techniques Used: Activity Assay, Fluorescence, Mouse Assay, High Performance Liquid Chromatography, Mass Spectrometry, Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy, Isolation

    3) Product Images from "Epithelial Biology and Secretion: Inhibition of autotaxin alleviates inflammation and increases the expression of sodium-dependent glucose cotransporter 1 and Na+/H+ exchanger 3 in SAMP1/Fc mice"

    Article Title: Epithelial Biology and Secretion: Inhibition of autotaxin alleviates inflammation and increases the expression of sodium-dependent glucose cotransporter 1 and Na+/H+ exchanger 3 in SAMP1/Fc mice

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    doi: 10.1152/ajpgi.00215.2018

    Inhibition of autotaxin (ATX) upregulates Na + -dependent glucose transporter 1 (SGLT1) expression in the intestine of SAMP1/Fc mice. AKR and SAMP1/Fc mice were treated with PF-8380 (PF) or control (Con). A : SGLT1 mRNA expression was determined by quantitative
    Figure Legend Snippet: Inhibition of autotaxin (ATX) upregulates Na + -dependent glucose transporter 1 (SGLT1) expression in the intestine of SAMP1/Fc mice. AKR and SAMP1/Fc mice were treated with PF-8380 (PF) or control (Con). A : SGLT1 mRNA expression was determined by quantitative

    Techniques Used: Inhibition, Expressing, Mouse Assay

    Inhibition of autotaxin (ATX) attenuates inflammatory cytokine expression and immune cell migration in SAMP1/Fc mice. Ileal tissues were collected from AKR and SAMP1/Fc mice that were treated with PF-8380 (PF) or control (Con), and the mRNA expression
    Figure Legend Snippet: Inhibition of autotaxin (ATX) attenuates inflammatory cytokine expression and immune cell migration in SAMP1/Fc mice. Ileal tissues were collected from AKR and SAMP1/Fc mice that were treated with PF-8380 (PF) or control (Con), and the mRNA expression

    Techniques Used: Inhibition, Expressing, Migration, Mouse Assay

    Inhibition of autotaxin (ATX) enhances Na + /H + exchanger 3 (NHE3) membrane expression in SAMP1/Fc mice. A : NHE3 protein expression was determined by immunoblotting of ileal lysates. B : ileal sections of PF-8380 (PF)- or control (Con)-treated AKR and SAMP1/Fc
    Figure Legend Snippet: Inhibition of autotaxin (ATX) enhances Na + /H + exchanger 3 (NHE3) membrane expression in SAMP1/Fc mice. A : NHE3 protein expression was determined by immunoblotting of ileal lysates. B : ileal sections of PF-8380 (PF)- or control (Con)-treated AKR and SAMP1/Fc

    Techniques Used: Inhibition, Expressing, Mouse Assay

    PF-8380 (PF) treatment inhibits autotaxin (ATX) activity and increases weight gain of SAMP1/Fc mice. Ileal scrapes were isolated from 4-, 8-, and 24-wk-old AKR and SAMP1/Fc mice, and the ATX mRNA expression was determined by quantitative RT-PCR ( A ). Data
    Figure Legend Snippet: PF-8380 (PF) treatment inhibits autotaxin (ATX) activity and increases weight gain of SAMP1/Fc mice. Ileal scrapes were isolated from 4-, 8-, and 24-wk-old AKR and SAMP1/Fc mice, and the ATX mRNA expression was determined by quantitative RT-PCR ( A ). Data

    Techniques Used: Activity Assay, Mouse Assay, Isolation, Expressing, Quantitative RT-PCR

    Inhibition of autotaxin (ATX) increases membrane expression of sucrase-isomaltase (SI) in intestinal epithelial cells in SAMP1/Fc mice. A : filter-grown Caco-2 cells were treated from the basolateral side with 50 ng/ml IL-4, IL-13, or IL-4 + IL-13 for
    Figure Legend Snippet: Inhibition of autotaxin (ATX) increases membrane expression of sucrase-isomaltase (SI) in intestinal epithelial cells in SAMP1/Fc mice. A : filter-grown Caco-2 cells were treated from the basolateral side with 50 ng/ml IL-4, IL-13, or IL-4 + IL-13 for

    Techniques Used: Inhibition, Expressing, Mouse Assay

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    Echelon Biosciences autotaxin activity
    Elevated <t>autotaxin</t> (ATX) activity following ischemia and reperfusion (I/R). A , Triphenyl tetrazolium chloride staining of mouse brain slices and respective infarct volume (percentage of the hemisphere) measured in sham and I/R mice. B , Mouse brain cryosections stained for microglia (Iba1) in sham and I/R mice ipsilateral cortex. Bar=200 µm. C , ATX mRNA expression was measured in ipsilateral cortex brain tissue lysate from sham and I/R mice. D , Enzymatic activity test for ATX measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU) in sham and I/R mouse brain slices. E , Lysophosphatidylcholine subspecies in plasma from sham and I/R mice, measured with liquid chromatography–mass spectrometry, quantified relative to 18:1 LPC. All values are mean±SD compared with the sham group using Mann‐Whitney U test. DAPI indicates 4′,6‐diamidino‐2‐phenylindole. NI, near‐infrared.
    Autotaxin Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/autotaxin activity/product/Echelon Biosciences
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    autotaxin activity - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

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    Elevated autotaxin (ATX) activity following ischemia and reperfusion (I/R). A , Triphenyl tetrazolium chloride staining of mouse brain slices and respective infarct volume (percentage of the hemisphere) measured in sham and I/R mice. B , Mouse brain cryosections stained for microglia (Iba1) in sham and I/R mice ipsilateral cortex. Bar=200 µm. C , ATX mRNA expression was measured in ipsilateral cortex brain tissue lysate from sham and I/R mice. D , Enzymatic activity test for ATX measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU) in sham and I/R mouse brain slices. E , Lysophosphatidylcholine subspecies in plasma from sham and I/R mice, measured with liquid chromatography–mass spectrometry, quantified relative to 18:1 LPC. All values are mean±SD compared with the sham group using Mann‐Whitney U test. DAPI indicates 4′,6‐diamidino‐2‐phenylindole. NI, near‐infrared.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Disrupted Blood‐Brain Barrier and Mitochondrial Impairment by Autotaxin–Lysophosphatidic Acid Axis in Postischemic Stroke

    doi: 10.1161/JAHA.121.021511

    Figure Lengend Snippet: Elevated autotaxin (ATX) activity following ischemia and reperfusion (I/R). A , Triphenyl tetrazolium chloride staining of mouse brain slices and respective infarct volume (percentage of the hemisphere) measured in sham and I/R mice. B , Mouse brain cryosections stained for microglia (Iba1) in sham and I/R mice ipsilateral cortex. Bar=200 µm. C , ATX mRNA expression was measured in ipsilateral cortex brain tissue lysate from sham and I/R mice. D , Enzymatic activity test for ATX measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU) in sham and I/R mouse brain slices. E , Lysophosphatidylcholine subspecies in plasma from sham and I/R mice, measured with liquid chromatography–mass spectrometry, quantified relative to 18:1 LPC. All values are mean±SD compared with the sham group using Mann‐Whitney U test. DAPI indicates 4′,6‐diamidino‐2‐phenylindole. NI, near‐infrared.

    Article Snippet: Autotaxin activity in the mouse brain and brain slices was analyzed using a fluorogenic analog of lysophosphatidylcholine (AR‐2), enabling in vivo visualization of autotaxin activity.

    Techniques: Activity Assay, Staining, Mouse Assay, Expressing, Fluorescence, Liquid Chromatography, Mass Spectrometry, MANN-WHITNEY

    Autotaxin (ATX) inhibitors in middle cerebral artery occlusion decrease ATX activity and lysophosphatidic acid (LPA). A , Enzymatic activity test for ATX, measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU), in sham, ischemia and reperfusion (I/R), HA130, and PF8380 mouse brain slices. B , Lysophosphatidylcholine (LPC) subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with high‐performance liquid chromatography–tandem mass spectrometry (liquid chromatography–mass spectrometry [LC‐MS]) quantified relative to 18:1 LPC. C , LPA subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with LC‐MS, quantified relative to 18:1 LPA. D and E , Graph represents oxygen consumption rate (OCR) (pmol/min per µg protein) measurements in isolated mitochondria from sham, I/R, HA130, PF8380, or BrP‐LPA mouse brain tissue at baseline and after sequential addition of oligomycin, carbonyl cyanide p‐trifluoro‐methoxyphenyl hydrazone (FCCP), and antimycin A+rotenone. F , Spare respiratory capacity, ATP turnover, and maximum respiration values analyzed in sham, I/R, HA130, PF8380, or BrP‐LPA mice. All values are mean±SD. * P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Disrupted Blood‐Brain Barrier and Mitochondrial Impairment by Autotaxin–Lysophosphatidic Acid Axis in Postischemic Stroke

    doi: 10.1161/JAHA.121.021511

    Figure Lengend Snippet: Autotaxin (ATX) inhibitors in middle cerebral artery occlusion decrease ATX activity and lysophosphatidic acid (LPA). A , Enzymatic activity test for ATX, measured with AR‐2 fluorescence, quantified as relative fluorescence unit (RFU), in sham, ischemia and reperfusion (I/R), HA130, and PF8380 mouse brain slices. B , Lysophosphatidylcholine (LPC) subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with high‐performance liquid chromatography–tandem mass spectrometry (liquid chromatography–mass spectrometry [LC‐MS]) quantified relative to 18:1 LPC. C , LPA subspecies in plasma from sham, I/R, HA130, and PF8380 mice measured with LC‐MS, quantified relative to 18:1 LPA. D and E , Graph represents oxygen consumption rate (OCR) (pmol/min per µg protein) measurements in isolated mitochondria from sham, I/R, HA130, PF8380, or BrP‐LPA mouse brain tissue at baseline and after sequential addition of oligomycin, carbonyl cyanide p‐trifluoro‐methoxyphenyl hydrazone (FCCP), and antimycin A+rotenone. F , Spare respiratory capacity, ATP turnover, and maximum respiration values analyzed in sham, I/R, HA130, PF8380, or BrP‐LPA mice. All values are mean±SD. * P

    Article Snippet: Autotaxin activity in the mouse brain and brain slices was analyzed using a fluorogenic analog of lysophosphatidylcholine (AR‐2), enabling in vivo visualization of autotaxin activity.

    Techniques: Activity Assay, Fluorescence, Mouse Assay, High Performance Liquid Chromatography, Mass Spectrometry, Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy, Isolation

    Inhibition of autotaxin (ATX) upregulates Na + -dependent glucose transporter 1 (SGLT1) expression in the intestine of SAMP1/Fc mice. AKR and SAMP1/Fc mice were treated with PF-8380 (PF) or control (Con). A : SGLT1 mRNA expression was determined by quantitative

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Epithelial Biology and Secretion: Inhibition of autotaxin alleviates inflammation and increases the expression of sodium-dependent glucose cotransporter 1 and Na+/H+ exchanger 3 in SAMP1/Fc mice

    doi: 10.1152/ajpgi.00215.2018

    Figure Lengend Snippet: Inhibition of autotaxin (ATX) upregulates Na + -dependent glucose transporter 1 (SGLT1) expression in the intestine of SAMP1/Fc mice. AKR and SAMP1/Fc mice were treated with PF-8380 (PF) or control (Con). A : SGLT1 mRNA expression was determined by quantitative

    Article Snippet: Serum was then prepared, and ATX activity was measured by using Autotaxin Activity Assay kit (Echelon Biosciences, Inc., Salt Lake, UT) according to the manufacturer’s instructions.

    Techniques: Inhibition, Expressing, Mouse Assay

    Inhibition of autotaxin (ATX) attenuates inflammatory cytokine expression and immune cell migration in SAMP1/Fc mice. Ileal tissues were collected from AKR and SAMP1/Fc mice that were treated with PF-8380 (PF) or control (Con), and the mRNA expression

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Epithelial Biology and Secretion: Inhibition of autotaxin alleviates inflammation and increases the expression of sodium-dependent glucose cotransporter 1 and Na+/H+ exchanger 3 in SAMP1/Fc mice

    doi: 10.1152/ajpgi.00215.2018

    Figure Lengend Snippet: Inhibition of autotaxin (ATX) attenuates inflammatory cytokine expression and immune cell migration in SAMP1/Fc mice. Ileal tissues were collected from AKR and SAMP1/Fc mice that were treated with PF-8380 (PF) or control (Con), and the mRNA expression

    Article Snippet: Serum was then prepared, and ATX activity was measured by using Autotaxin Activity Assay kit (Echelon Biosciences, Inc., Salt Lake, UT) according to the manufacturer’s instructions.

    Techniques: Inhibition, Expressing, Migration, Mouse Assay

    Inhibition of autotaxin (ATX) enhances Na + /H + exchanger 3 (NHE3) membrane expression in SAMP1/Fc mice. A : NHE3 protein expression was determined by immunoblotting of ileal lysates. B : ileal sections of PF-8380 (PF)- or control (Con)-treated AKR and SAMP1/Fc

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Epithelial Biology and Secretion: Inhibition of autotaxin alleviates inflammation and increases the expression of sodium-dependent glucose cotransporter 1 and Na+/H+ exchanger 3 in SAMP1/Fc mice

    doi: 10.1152/ajpgi.00215.2018

    Figure Lengend Snippet: Inhibition of autotaxin (ATX) enhances Na + /H + exchanger 3 (NHE3) membrane expression in SAMP1/Fc mice. A : NHE3 protein expression was determined by immunoblotting of ileal lysates. B : ileal sections of PF-8380 (PF)- or control (Con)-treated AKR and SAMP1/Fc

    Article Snippet: Serum was then prepared, and ATX activity was measured by using Autotaxin Activity Assay kit (Echelon Biosciences, Inc., Salt Lake, UT) according to the manufacturer’s instructions.

    Techniques: Inhibition, Expressing, Mouse Assay

    PF-8380 (PF) treatment inhibits autotaxin (ATX) activity and increases weight gain of SAMP1/Fc mice. Ileal scrapes were isolated from 4-, 8-, and 24-wk-old AKR and SAMP1/Fc mice, and the ATX mRNA expression was determined by quantitative RT-PCR ( A ). Data

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Epithelial Biology and Secretion: Inhibition of autotaxin alleviates inflammation and increases the expression of sodium-dependent glucose cotransporter 1 and Na+/H+ exchanger 3 in SAMP1/Fc mice

    doi: 10.1152/ajpgi.00215.2018

    Figure Lengend Snippet: PF-8380 (PF) treatment inhibits autotaxin (ATX) activity and increases weight gain of SAMP1/Fc mice. Ileal scrapes were isolated from 4-, 8-, and 24-wk-old AKR and SAMP1/Fc mice, and the ATX mRNA expression was determined by quantitative RT-PCR ( A ). Data

    Article Snippet: Serum was then prepared, and ATX activity was measured by using Autotaxin Activity Assay kit (Echelon Biosciences, Inc., Salt Lake, UT) according to the manufacturer’s instructions.

    Techniques: Activity Assay, Mouse Assay, Isolation, Expressing, Quantitative RT-PCR

    Inhibition of autotaxin (ATX) increases membrane expression of sucrase-isomaltase (SI) in intestinal epithelial cells in SAMP1/Fc mice. A : filter-grown Caco-2 cells were treated from the basolateral side with 50 ng/ml IL-4, IL-13, or IL-4 + IL-13 for

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Epithelial Biology and Secretion: Inhibition of autotaxin alleviates inflammation and increases the expression of sodium-dependent glucose cotransporter 1 and Na+/H+ exchanger 3 in SAMP1/Fc mice

    doi: 10.1152/ajpgi.00215.2018

    Figure Lengend Snippet: Inhibition of autotaxin (ATX) increases membrane expression of sucrase-isomaltase (SI) in intestinal epithelial cells in SAMP1/Fc mice. A : filter-grown Caco-2 cells were treated from the basolateral side with 50 ng/ml IL-4, IL-13, or IL-4 + IL-13 for

    Article Snippet: Serum was then prepared, and ATX activity was measured by using Autotaxin Activity Assay kit (Echelon Biosciences, Inc., Salt Lake, UT) according to the manufacturer’s instructions.

    Techniques: Inhibition, Expressing, Mouse Assay