Journal: The EMBO Journal
Article Title: PIP2 depletion and altered endocytosis caused by expression of Alzheimer's disease‐protective variant PLCγ2 R522
doi: 10.15252/embj.2020105603
Figure Lengend Snippet: A–C A mass ELISA was used to detect specific PIP species after exposure to anti‐FcγRII/III (2.4G2) with or without 3‐a‐aminocholestane (SHIP 1 inhibitor), SF1670 (PTEN inhibitor) and LY294002 (PI‐3K inhibitor). In M‐MOP cells (left panel) and primary microglia (right panel), PI(4,5)P 2 (A), PI(3,4)P 2 (B) and PIP 3 (C) were detected. All data show the mean ± SD of three independent experiments and were analysed by two‐way ANOVA (log‐transformed data were used in C) with Sidak’s multiple comparison tests performed. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001 (blue: P522 and red: R522).
Article Snippet: PIP isoforms were quantified from extracted lipids using PI(4,5)P2 Mass ELISA (K‐4500), PI(3,4)P2 Mass ELISA (K‐3800) and PIP3 Mass ELISA (K‐2500s) from Echelon Biosciences using the manufacturer’s instructions.
Techniques: Enzyme-linked Immunosorbent Assay, Transformation Assay