sphk activity (Echelon Biosciences)


Structured Review
Sphk Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphk activity/product/Echelon Biosciences
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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sphk activity (Echelon Biosciences)


Structured Review
Sphk Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphk activity/product/Echelon Biosciences
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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sphingosine kinase (Echelon Biosciences)


Structured Review
Sphingosine Kinase, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphingosine kinase/product/Echelon Biosciences
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
sphingosine kinase activity assay kit (Echelon Biosciences)


Structured Review
Sphingosine Kinase Activity Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphingosine kinase activity assay kit/product/Echelon Biosciences
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rat sphk1 activity elisa kits (Echelon Biosciences)


Structured Review
Rat Sphk1 Activity Elisa Kits, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat sphk1 activity elisa kits/product/Echelon Biosciences
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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k 3500 (Echelon Biosciences)


Structured Review
K 3500, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/k 3500/product/Echelon Biosciences
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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sphk1 activity assay (Echelon Biosciences)


Structured Review

Sphk1 Activity Assay, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphk1 activity assay/product/Echelon Biosciences
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "aPC/PAR1 confers endothelial anti-apoptotic activity via a discrete β-arrestin-2 mediated SphK1-S1PR1-Akt signaling axis"
Article Title: aPC/PAR1 confers endothelial anti-apoptotic activity via a discrete β-arrestin-2 mediated SphK1-S1PR1-Akt signaling axis
Journal: bioRxiv
doi: 10.1101/2021.03.27.437291

Figure Legend Snippet: (A) EA.hy926 cells were stimulate with or without aPC for 180 min, phospho-SphK1 was detected by immunoblotting. Data (mean ± S.D., n = 3) were analyzed by Student’s t-test. (B) EA.hy926 cells were stimulated with or without 20 nM aPC for 30 min, cells were lysed and subcellular fractionation performed. SphK1, the cytoplasmic control (GAPDH) and the membrane control (Na + /K + ATPase) were detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (C) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h, and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity assessed by using the Echelon SphK1 activity assay. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (D) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h and stimulated with or without 20 nM aPC for various times, phospho-Akt-S473 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (E) HUVECs were treated and analyzed as described in D. ****, P <0.0001, ***, P <0.001, **, P <0.01, and *, P <0.05.
Techniques Used: Western Blot, Fractionation, Activity Assay

Figure Legend Snippet: (A) EA.hy926 cells were treated with 20 nM aPC for various times. Cells were lysed and SphK1 activity was measured using an Echelon SphK1 activity assay. The luminescence of an ATP standard curve and aPC treated cells was deteremined and the raw values are shown. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. **, P <0.001. (B) EA.hy926 cells were pretreated with or without 100 nM PF-543 and stimulated with or without 20 nM of aPC for various times. Phospho-ERK1/2 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (B) Same as A but in HUVECs.
Techniques Used: Activity Assay, Western Blot

Figure Legend Snippet: ( A) EA.hy926 cells were treated with either non-specific (ns) siRNA or β-arr2 siRNA, serum-starved and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity was assessed. Data (mean ± S.D., n = 5) was analyzed by Student’s t-test. β-arr2 knockdown was verified by immunoblotting. (B) EA.hy926 cells were treated with either ns siRNA or Dvl-2 siRNA and SphK1 activity determined. Dvl-2 knockdown was confirmed by immunoblotting. Data (mean ± S.D., n = 5) was analyzed by Student’s t-test, ****, P <0.0001 and ***, P <0.001.
Techniques Used: Activity Assay, Western Blot

Figure Legend Snippet: Activation of PAR1 by aPC bound to its’ co-receptor EPCR promotes β-arr2-dependent SphK1 transactivation of S1PR1 that results in Akt at S473 phosphorylation, which protects endothelial cells from TNF-α induced cell death. SphK1 activation is associated with β-arr2-mediated phosphorylation, translocation to the plasma membrane following aPC stimulation.
Techniques Used: Activation Assay, Translocation Assay
sphingosine kinase activity assay kit (Echelon Biosciences)


Structured Review
Sphingosine Kinase Activity Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphingosine kinase activity assay kit/product/Echelon Biosciences
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
sphingosine kinase activity assay kit (Echelon Biosciences)


Structured Review
Sphingosine Kinase Activity Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphingosine kinase activity assay kit/product/Echelon Biosciences
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
sphk activity (Echelon Biosciences)


Structured Review

Sphk Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphk activity/product/Echelon Biosciences
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Human keratinocytes use sphingosine 1-phosphate and its receptors to communicate S. aureus invasion and activate host defense."
Article Title: Human keratinocytes use sphingosine 1-phosphate and its receptors to communicate S. aureus invasion and activate host defense.
Journal: The Journal of investigative dermatology
doi: 10.1016/j.jid.2019.02.010

Figure Legend Snippet: (a) mRNA expression levels of SPHK1 and 2 in NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 4hrs. Data is shown as the fold of TSB control. (b) ATP consumptions indicating SPHK activity in NHEKs incubated with 50 μl/ml TSB or sa113 supernatant for 24hrs. (c) S1P secreted into culture medium of NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 48hrs.
Techniques Used: Expressing, Incubation, Activity Assay
sphk activity (Echelon Biosciences)


Structured Review

Sphk Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sphk activity/product/Echelon Biosciences
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Human keratinocytes use sphingosine 1-phosphate and its receptors to communicate S. aureus invasion and activate host defense."
Article Title: Human keratinocytes use sphingosine 1-phosphate and its receptors to communicate S. aureus invasion and activate host defense.
Journal: The Journal of investigative dermatology
doi: 10.1016/j.jid.2019.02.010

Figure Legend Snippet: (a) mRNA expression levels of SPHK1 and 2 in NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 4hrs. Data is shown as the fold of TSB control. (b) ATP consumptions indicating SPHK activity in NHEKs incubated with 50 μl/ml TSB or sa113 supernatant for 24hrs. (c) S1P secreted into culture medium of NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 48hrs.
Techniques Used: Expressing, Incubation, Activity Assay