sphk activity  (Echelon Biosciences)


Bioz Verified Symbol Echelon Biosciences is a verified supplier
Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Echelon Biosciences sphk activity
    Sphk Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphk activity/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphk activity - by Bioz Stars, 2023-02
    95/100 stars

    Images

    sphk activity  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Echelon Biosciences sphk activity
    Sphk Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphk activity/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphk activity - by Bioz Stars, 2023-02
    95/100 stars

    Images

    sphingosine kinase  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Echelon Biosciences sphingosine kinase
    Sphingosine Kinase, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphingosine kinase/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphingosine kinase - by Bioz Stars, 2023-02
    95/100 stars

    Images

    sphingosine kinase activity assay kit  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Echelon Biosciences sphingosine kinase activity assay kit
    Sphingosine Kinase Activity Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphingosine kinase activity assay kit/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphingosine kinase activity assay kit - by Bioz Stars, 2023-02
    95/100 stars

    Images

    rat sphk1 activity elisa kits  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Echelon Biosciences rat sphk1 activity elisa kits
    Rat Sphk1 Activity Elisa Kits, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat sphk1 activity elisa kits/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat sphk1 activity elisa kits - by Bioz Stars, 2023-02
    95/100 stars

    Images

    k 3500  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Echelon Biosciences k 3500
    K 3500, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k 3500/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k 3500 - by Bioz Stars, 2023-02
    95/100 stars

    Images

    sphk1 activity assay  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Echelon Biosciences sphk1 activity assay
    (A) EA.hy926 cells were stimulate with or without aPC for 180 min, <t>phospho-SphK1</t> was detected by immunoblotting. Data (mean ± S.D., n = 3) were analyzed by Student’s t-test. (B) EA.hy926 cells were stimulated with or without 20 nM aPC for 30 min, cells were lysed and subcellular fractionation performed. SphK1, the cytoplasmic control (GAPDH) and the membrane control (Na + /K + ATPase) were detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (C) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h, and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity assessed by using the Echelon SphK1 activity assay. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (D) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h and stimulated with or without 20 nM aPC for various times, phospho-Akt-S473 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (E) HUVECs were treated and analyzed as described in D. ****, P <0.0001, ***, P <0.001, **, P <0.01, and *, P <0.05.
    Sphk1 Activity Assay, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphk1 activity assay/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphk1 activity assay - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "aPC/PAR1 confers endothelial anti-apoptotic activity via a discrete β-arrestin-2 mediated SphK1-S1PR1-Akt signaling axis"

    Article Title: aPC/PAR1 confers endothelial anti-apoptotic activity via a discrete β-arrestin-2 mediated SphK1-S1PR1-Akt signaling axis

    Journal: bioRxiv

    doi: 10.1101/2021.03.27.437291

    (A) EA.hy926 cells were stimulate with or without aPC for 180 min, phospho-SphK1 was detected by immunoblotting. Data (mean ± S.D., n = 3) were analyzed by Student’s t-test. (B) EA.hy926 cells were stimulated with or without 20 nM aPC for 30 min, cells were lysed and subcellular fractionation performed. SphK1, the cytoplasmic control (GAPDH) and the membrane control (Na + /K + ATPase) were detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (C) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h, and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity assessed by using the Echelon SphK1 activity assay. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (D) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h and stimulated with or without 20 nM aPC for various times, phospho-Akt-S473 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (E) HUVECs were treated and analyzed as described in D. ****, P <0.0001, ***, P <0.001, **, P <0.01, and *, P <0.05.
    Figure Legend Snippet: (A) EA.hy926 cells were stimulate with or without aPC for 180 min, phospho-SphK1 was detected by immunoblotting. Data (mean ± S.D., n = 3) were analyzed by Student’s t-test. (B) EA.hy926 cells were stimulated with or without 20 nM aPC for 30 min, cells were lysed and subcellular fractionation performed. SphK1, the cytoplasmic control (GAPDH) and the membrane control (Na + /K + ATPase) were detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (C) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h, and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity assessed by using the Echelon SphK1 activity assay. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (D) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h and stimulated with or without 20 nM aPC for various times, phospho-Akt-S473 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (E) HUVECs were treated and analyzed as described in D. ****, P <0.0001, ***, P <0.001, **, P <0.01, and *, P <0.05.

    Techniques Used: Western Blot, Fractionation, Activity Assay

    (A) EA.hy926 cells were treated with 20 nM aPC for various times. Cells were lysed and SphK1 activity was measured using an Echelon SphK1 activity assay. The luminescence of an ATP standard curve and aPC treated cells was deteremined and the raw values are shown. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. **, P <0.001. (B) EA.hy926 cells were pretreated with or without 100 nM PF-543 and stimulated with or without 20 nM of aPC for various times. Phospho-ERK1/2 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (B) Same as A but in HUVECs.
    Figure Legend Snippet: (A) EA.hy926 cells were treated with 20 nM aPC for various times. Cells were lysed and SphK1 activity was measured using an Echelon SphK1 activity assay. The luminescence of an ATP standard curve and aPC treated cells was deteremined and the raw values are shown. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. **, P <0.001. (B) EA.hy926 cells were pretreated with or without 100 nM PF-543 and stimulated with or without 20 nM of aPC for various times. Phospho-ERK1/2 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (B) Same as A but in HUVECs.

    Techniques Used: Activity Assay, Western Blot

    ( A) EA.hy926 cells were treated with either non-specific (ns) siRNA or β-arr2 siRNA, serum-starved and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity was assessed. Data (mean ± S.D., n = 5) was analyzed by Student’s t-test. β-arr2 knockdown was verified by immunoblotting. (B) EA.hy926 cells were treated with either ns siRNA or Dvl-2 siRNA and SphK1 activity determined. Dvl-2 knockdown was confirmed by immunoblotting. Data (mean ± S.D., n = 5) was analyzed by Student’s t-test, ****, P <0.0001 and ***, P <0.001.
    Figure Legend Snippet: ( A) EA.hy926 cells were treated with either non-specific (ns) siRNA or β-arr2 siRNA, serum-starved and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity was assessed. Data (mean ± S.D., n = 5) was analyzed by Student’s t-test. β-arr2 knockdown was verified by immunoblotting. (B) EA.hy926 cells were treated with either ns siRNA or Dvl-2 siRNA and SphK1 activity determined. Dvl-2 knockdown was confirmed by immunoblotting. Data (mean ± S.D., n = 5) was analyzed by Student’s t-test, ****, P <0.0001 and ***, P <0.001.

    Techniques Used: Activity Assay, Western Blot

    Activation of PAR1 by aPC bound to its’ co-receptor EPCR promotes β-arr2-dependent SphK1 transactivation of S1PR1 that results in Akt at S473 phosphorylation, which protects endothelial cells from TNF-α induced cell death. SphK1 activation is associated with β-arr2-mediated phosphorylation, translocation to the plasma membrane following aPC stimulation.
    Figure Legend Snippet: Activation of PAR1 by aPC bound to its’ co-receptor EPCR promotes β-arr2-dependent SphK1 transactivation of S1PR1 that results in Akt at S473 phosphorylation, which protects endothelial cells from TNF-α induced cell death. SphK1 activation is associated with β-arr2-mediated phosphorylation, translocation to the plasma membrane following aPC stimulation.

    Techniques Used: Activation Assay, Translocation Assay

    sphingosine kinase activity assay kit  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Echelon Biosciences sphingosine kinase activity assay kit
    Sphingosine Kinase Activity Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphingosine kinase activity assay kit/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphingosine kinase activity assay kit - by Bioz Stars, 2023-02
    95/100 stars

    Images

    sphingosine kinase activity assay kit  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Echelon Biosciences sphingosine kinase activity assay kit
    Sphingosine Kinase Activity Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphingosine kinase activity assay kit/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphingosine kinase activity assay kit - by Bioz Stars, 2023-02
    95/100 stars

    Images

    sphk activity  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Echelon Biosciences sphk activity
    (a) mRNA expression levels of SPHK1 and 2 in NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 4hrs. Data is shown as the fold of TSB control. (b) ATP consumptions indicating <t>SPHK</t> <t>activity</t> in NHEKs incubated with 50 μl/ml TSB or sa113 supernatant for 24hrs. (c) S1P secreted into culture medium of NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 48hrs.
    Sphk Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphk activity/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphk activity - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Human keratinocytes use sphingosine 1-phosphate and its receptors to communicate S. aureus invasion and activate host defense."

    Article Title: Human keratinocytes use sphingosine 1-phosphate and its receptors to communicate S. aureus invasion and activate host defense.

    Journal: The Journal of investigative dermatology

    doi: 10.1016/j.jid.2019.02.010

    (a) mRNA expression levels of SPHK1 and 2 in NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 4hrs. Data is shown as the fold of TSB control. (b) ATP consumptions indicating SPHK activity in NHEKs incubated with 50 μl/ml TSB or sa113 supernatant for 24hrs. (c) S1P secreted into culture medium of NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 48hrs.
    Figure Legend Snippet: (a) mRNA expression levels of SPHK1 and 2 in NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 4hrs. Data is shown as the fold of TSB control. (b) ATP consumptions indicating SPHK activity in NHEKs incubated with 50 μl/ml TSB or sa113 supernatant for 24hrs. (c) S1P secreted into culture medium of NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 48hrs.

    Techniques Used: Expressing, Incubation, Activity Assay

    sphk activity  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Echelon Biosciences sphk activity
    (a) mRNA expression levels of SPHK1 and 2 in NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 4hrs. Data is shown as the fold of TSB control. (b) ATP consumptions indicating <t>SPHK</t> <t>activity</t> in NHEKs incubated with 50 μl/ml TSB or sa113 supernatant for 24hrs. (c) S1P secreted into culture medium of NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 48hrs.
    Sphk Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphk activity/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphk activity - by Bioz Stars, 2023-02
    95/100 stars

    Images

    1) Product Images from "Human keratinocytes use sphingosine 1-phosphate and its receptors to communicate S. aureus invasion and activate host defense."

    Article Title: Human keratinocytes use sphingosine 1-phosphate and its receptors to communicate S. aureus invasion and activate host defense.

    Journal: The Journal of investigative dermatology

    doi: 10.1016/j.jid.2019.02.010

    (a) mRNA expression levels of SPHK1 and 2 in NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 4hrs. Data is shown as the fold of TSB control. (b) ATP consumptions indicating SPHK activity in NHEKs incubated with 50 μl/ml TSB or sa113 supernatant for 24hrs. (c) S1P secreted into culture medium of NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 48hrs.
    Figure Legend Snippet: (a) mRNA expression levels of SPHK1 and 2 in NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 4hrs. Data is shown as the fold of TSB control. (b) ATP consumptions indicating SPHK activity in NHEKs incubated with 50 μl/ml TSB or sa113 supernatant for 24hrs. (c) S1P secreted into culture medium of NHEKs incubated with 50 μl/ml of TSB or sa113 supernatant for 48hrs.

    Techniques Used: Expressing, Incubation, Activity Assay

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Echelon Biosciences sphk activity
    Sphk Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphk activity/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphk activity - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    95
    Echelon Biosciences sphingosine kinase
    Sphingosine Kinase, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphingosine kinase/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphingosine kinase - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    95
    Echelon Biosciences sphingosine kinase activity assay kit
    Sphingosine Kinase Activity Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphingosine kinase activity assay kit/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphingosine kinase activity assay kit - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    95
    Echelon Biosciences rat sphk1 activity elisa kits
    Rat Sphk1 Activity Elisa Kits, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat sphk1 activity elisa kits/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat sphk1 activity elisa kits - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    95
    Echelon Biosciences k 3500
    K 3500, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k 3500/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k 3500 - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    95
    Echelon Biosciences sphk1 activity assay
    (A) EA.hy926 cells were stimulate with or without aPC for 180 min, <t>phospho-SphK1</t> was detected by immunoblotting. Data (mean ± S.D., n = 3) were analyzed by Student’s t-test. (B) EA.hy926 cells were stimulated with or without 20 nM aPC for 30 min, cells were lysed and subcellular fractionation performed. SphK1, the cytoplasmic control (GAPDH) and the membrane control (Na + /K + ATPase) were detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (C) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h, and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity assessed by using the Echelon SphK1 activity assay. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (D) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h and stimulated with or without 20 nM aPC for various times, phospho-Akt-S473 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (E) HUVECs were treated and analyzed as described in D. ****, P <0.0001, ***, P <0.001, **, P <0.01, and *, P <0.05.
    Sphk1 Activity Assay, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphk1 activity assay/product/Echelon Biosciences
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphk1 activity assay - by Bioz Stars, 2023-02
    95/100 stars
      Buy from Supplier

    Image Search Results


    (A) EA.hy926 cells were stimulate with or without aPC for 180 min, phospho-SphK1 was detected by immunoblotting. Data (mean ± S.D., n = 3) were analyzed by Student’s t-test. (B) EA.hy926 cells were stimulated with or without 20 nM aPC for 30 min, cells were lysed and subcellular fractionation performed. SphK1, the cytoplasmic control (GAPDH) and the membrane control (Na + /K + ATPase) were detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (C) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h, and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity assessed by using the Echelon SphK1 activity assay. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (D) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h and stimulated with or without 20 nM aPC for various times, phospho-Akt-S473 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (E) HUVECs were treated and analyzed as described in D. ****, P <0.0001, ***, P <0.001, **, P <0.01, and *, P <0.05.

    Journal: bioRxiv

    Article Title: aPC/PAR1 confers endothelial anti-apoptotic activity via a discrete β-arrestin-2 mediated SphK1-S1PR1-Akt signaling axis

    doi: 10.1101/2021.03.27.437291

    Figure Lengend Snippet: (A) EA.hy926 cells were stimulate with or without aPC for 180 min, phospho-SphK1 was detected by immunoblotting. Data (mean ± S.D., n = 3) were analyzed by Student’s t-test. (B) EA.hy926 cells were stimulated with or without 20 nM aPC for 30 min, cells were lysed and subcellular fractionation performed. SphK1, the cytoplasmic control (GAPDH) and the membrane control (Na + /K + ATPase) were detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (C) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h, and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity assessed by using the Echelon SphK1 activity assay. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. (D) EA.hy926 cells were pretreated with or without 100 nM PF-543 for 1 h and stimulated with or without 20 nM aPC for various times, phospho-Akt-S473 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (E) HUVECs were treated and analyzed as described in D. ****, P <0.0001, ***, P <0.001, **, P <0.01, and *, P <0.05.

    Article Snippet: Serum-starved cells stimulated with 20nM aPC for 15 min, washed with cold PBS and SphK1 activity assay performed according to the manufacturer instructions (Echelon, #K-3500).

    Techniques: Western Blot, Fractionation, Activity Assay

    (A) EA.hy926 cells were treated with 20 nM aPC for various times. Cells were lysed and SphK1 activity was measured using an Echelon SphK1 activity assay. The luminescence of an ATP standard curve and aPC treated cells was deteremined and the raw values are shown. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. **, P <0.001. (B) EA.hy926 cells were pretreated with or without 100 nM PF-543 and stimulated with or without 20 nM of aPC for various times. Phospho-ERK1/2 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (B) Same as A but in HUVECs.

    Journal: bioRxiv

    Article Title: aPC/PAR1 confers endothelial anti-apoptotic activity via a discrete β-arrestin-2 mediated SphK1-S1PR1-Akt signaling axis

    doi: 10.1101/2021.03.27.437291

    Figure Lengend Snippet: (A) EA.hy926 cells were treated with 20 nM aPC for various times. Cells were lysed and SphK1 activity was measured using an Echelon SphK1 activity assay. The luminescence of an ATP standard curve and aPC treated cells was deteremined and the raw values are shown. Data (mean ± S.D., n = 3) was analyzed by Student’s t-test. **, P <0.001. (B) EA.hy926 cells were pretreated with or without 100 nM PF-543 and stimulated with or without 20 nM of aPC for various times. Phospho-ERK1/2 was detected by immunoblotting. Data (mean ± S.D., n = 3) was analyzed by two-way ANOVA. (B) Same as A but in HUVECs.

    Article Snippet: Serum-starved cells stimulated with 20nM aPC for 15 min, washed with cold PBS and SphK1 activity assay performed according to the manufacturer instructions (Echelon, #K-3500).

    Techniques: Activity Assay, Western Blot

    ( A) EA.hy926 cells were treated with either non-specific (ns) siRNA or β-arr2 siRNA, serum-starved and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity was assessed. Data (mean ± S.D., n = 5) was analyzed by Student’s t-test. β-arr2 knockdown was verified by immunoblotting. (B) EA.hy926 cells were treated with either ns siRNA or Dvl-2 siRNA and SphK1 activity determined. Dvl-2 knockdown was confirmed by immunoblotting. Data (mean ± S.D., n = 5) was analyzed by Student’s t-test, ****, P <0.0001 and ***, P <0.001.

    Journal: bioRxiv

    Article Title: aPC/PAR1 confers endothelial anti-apoptotic activity via a discrete β-arrestin-2 mediated SphK1-S1PR1-Akt signaling axis

    doi: 10.1101/2021.03.27.437291

    Figure Lengend Snippet: ( A) EA.hy926 cells were treated with either non-specific (ns) siRNA or β-arr2 siRNA, serum-starved and then treated with 20 nM of aPC for 15 min. Cells were lysed and SphK1 activity was assessed. Data (mean ± S.D., n = 5) was analyzed by Student’s t-test. β-arr2 knockdown was verified by immunoblotting. (B) EA.hy926 cells were treated with either ns siRNA or Dvl-2 siRNA and SphK1 activity determined. Dvl-2 knockdown was confirmed by immunoblotting. Data (mean ± S.D., n = 5) was analyzed by Student’s t-test, ****, P <0.0001 and ***, P <0.001.

    Article Snippet: Serum-starved cells stimulated with 20nM aPC for 15 min, washed with cold PBS and SphK1 activity assay performed according to the manufacturer instructions (Echelon, #K-3500).

    Techniques: Activity Assay, Western Blot

    Activation of PAR1 by aPC bound to its’ co-receptor EPCR promotes β-arr2-dependent SphK1 transactivation of S1PR1 that results in Akt at S473 phosphorylation, which protects endothelial cells from TNF-α induced cell death. SphK1 activation is associated with β-arr2-mediated phosphorylation, translocation to the plasma membrane following aPC stimulation.

    Journal: bioRxiv

    Article Title: aPC/PAR1 confers endothelial anti-apoptotic activity via a discrete β-arrestin-2 mediated SphK1-S1PR1-Akt signaling axis

    doi: 10.1101/2021.03.27.437291

    Figure Lengend Snippet: Activation of PAR1 by aPC bound to its’ co-receptor EPCR promotes β-arr2-dependent SphK1 transactivation of S1PR1 that results in Akt at S473 phosphorylation, which protects endothelial cells from TNF-α induced cell death. SphK1 activation is associated with β-arr2-mediated phosphorylation, translocation to the plasma membrane following aPC stimulation.

    Article Snippet: Serum-starved cells stimulated with 20nM aPC for 15 min, washed with cold PBS and SphK1 activity assay performed according to the manufacturer instructions (Echelon, #K-3500).

    Techniques: Activation Assay, Translocation Assay