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competitive elisa  (Echelon Biosciences)


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    Echelon Biosciences competitive elisa
    Competitive Elisa, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Phage growth parameters obtained from the first lytic cycle of one step growth experiments with E.coli cultured in a chemostat at the dilution rates shown in Fig. : ( A ) Initial concentration of bacteria (X 0 ) and bacteriophages (P 0 ); ( B ) Concentration of infected bacteria (I X ) and generated phages (GF); ( C ) Latent period (LP) and Eclipse period (EP); ( D ) Adsorption constant (Ka); ( E ) Burst size (BS), which increased linearly with dilution rate (slope = 86.69 ± 3.98, R² = 0.985). The dashed Line indicates the regression fit, and dotted Lines represent the 95% confidence interval; (F) Bacteriophage fitness (F, red squares) decreased with decreasing dilution rate, in agreement with model predictions λ = λ max *D/(K λ +D) (λ max = 11.1 h −1 ; K λ =0.39 h −1 , black dashed line). ANOVA confirmed significant differences between all dilution rates ( p < 0.0001). Error bars indicate ± SD of three independent biological replicates

    Journal: Virology Journal

    Article Title: Bacteriophage T4 propagation in E.coli exposed to severe substrate limitation

    doi: 10.1186/s12985-025-02934-0

    Figure Lengend Snippet: Phage growth parameters obtained from the first lytic cycle of one step growth experiments with E.coli cultured in a chemostat at the dilution rates shown in Fig. : ( A ) Initial concentration of bacteria (X 0 ) and bacteriophages (P 0 ); ( B ) Concentration of infected bacteria (I X ) and generated phages (GF); ( C ) Latent period (LP) and Eclipse period (EP); ( D ) Adsorption constant (Ka); ( E ) Burst size (BS), which increased linearly with dilution rate (slope = 86.69 ± 3.98, R² = 0.985). The dashed Line indicates the regression fit, and dotted Lines represent the 95% confidence interval; (F) Bacteriophage fitness (F, red squares) decreased with decreasing dilution rate, in agreement with model predictions λ = λ max *D/(K λ +D) (λ max = 11.1 h −1 ; K λ =0.39 h −1 , black dashed line). ANOVA confirmed significant differences between all dilution rates ( p < 0.0001). Error bars indicate ± SD of three independent biological replicates

    Article Snippet: The bacterial strain Escherichia coli K-12 MG 1655 (DSM 18039), sourced from the Leibniz Institute DSMZ Germany, and the lytic bacteriophage T4 (DSM 4505) were employed in all experiments.

    Techniques: Cell Culture, Concentration Assay, Bacteria, Infection, Generated, Adsorption