k-1800  (Echelon Biosciences)


Bioz Verified Symbol Echelon Biosciences is a verified supplier
Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Echelon Biosciences k-1800
    K 1800, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k-1800/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k-1800 - by Bioz Stars, 2023-12
    94/100 stars

    Images

    neutral sphingomyelinase activity assay kit  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences neutral sphingomyelinase activity assay kit
    Changes in the activity ( A ) and protein expression ( B ) of neutral <t>sphingomyelinase</t> (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.
    Neutral Sphingomyelinase Activity Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutral sphingomyelinase activity assay kit/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neutral sphingomyelinase activity assay kit - by Bioz Stars, 2023-12
    93/100 stars

    Images

    1) Product Images from "Effect of Quercetin and Fingolimod, Alone or in Combination, on the Sphingolipid Metabolism in HepG2 Cells"

    Article Title: Effect of Quercetin and Fingolimod, Alone or in Combination, on the Sphingolipid Metabolism in HepG2 Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms232213916

    Changes in the activity ( A ) and protein expression ( B ) of neutral sphingomyelinase (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.
    Figure Legend Snippet: Changes in the activity ( A ) and protein expression ( B ) of neutral sphingomyelinase (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.

    Techniques Used: Activity Assay, Expressing, Western Blot

    neutral sphingomyelinase activity  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences neutral sphingomyelinase activity
    Changes in the activity ( A ) and protein expression ( B ) of neutral <t>sphingomyelinase</t> (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.
    Neutral Sphingomyelinase Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutral sphingomyelinase activity/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neutral sphingomyelinase activity - by Bioz Stars, 2023-12
    93/100 stars

    Images

    1) Product Images from "Effect of Quercetin and Fingolimod, Alone or in Combination, on the Sphingolipid Metabolism in HepG2 Cells"

    Article Title: Effect of Quercetin and Fingolimod, Alone or in Combination, on the Sphingolipid Metabolism in HepG2 Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms232213916

    Changes in the activity ( A ) and protein expression ( B ) of neutral sphingomyelinase (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.
    Figure Legend Snippet: Changes in the activity ( A ) and protein expression ( B ) of neutral sphingomyelinase (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.

    Techniques Used: Activity Assay, Expressing, Western Blot

    neutral sphingomyelinase activity assay kit  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences neutral sphingomyelinase activity assay kit
    Changes in the activity ( A ) and protein expression ( B ) of neutral <t>sphingomyelinase</t> (nSMase) in control (white bars) and resveratrol-treated (black bars) A549 cells. Values are expressed as % change of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (nSMase) are shown in the left part of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading; (ᵒ) stands for the values from each independent experiment, obtained by pooling of three replicate samples. Values represent means ± SD. The differences between the obtained values were not statistically significant.
    Neutral Sphingomyelinase Activity Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutral sphingomyelinase activity assay kit/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neutral sphingomyelinase activity assay kit - by Bioz Stars, 2023-12
    93/100 stars

    Images

    1) Product Images from "Resveratrol Affects Sphingolipid Metabolism in A549 Lung Adenocarcinoma Cells"

    Article Title: Resveratrol Affects Sphingolipid Metabolism in A549 Lung Adenocarcinoma Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms231810870

    Changes in the activity ( A ) and protein expression ( B ) of neutral sphingomyelinase (nSMase) in control (white bars) and resveratrol-treated (black bars) A549 cells. Values are expressed as % change of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (nSMase) are shown in the left part of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading; (ᵒ) stands for the values from each independent experiment, obtained by pooling of three replicate samples. Values represent means ± SD. The differences between the obtained values were not statistically significant.
    Figure Legend Snippet: Changes in the activity ( A ) and protein expression ( B ) of neutral sphingomyelinase (nSMase) in control (white bars) and resveratrol-treated (black bars) A549 cells. Values are expressed as % change of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (nSMase) are shown in the left part of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading; (ᵒ) stands for the values from each independent experiment, obtained by pooling of three replicate samples. Values represent means ± SD. The differences between the obtained values were not statistically significant.

    Techniques Used: Activity Assay, Expressing, Western Blot

    Schematic presentation of the effects of resveratrol on sphingolipid metabolism in lung cancer A549 cells. SM—sphingomyelin; CER—ceramide; SPH—sphingosine; S1P—sphingosine-1-phosphate; nSMase—neutral sphingomyelinase; ALCER—alkaline ceramidase; SK1—sphingosine kinase 1; CER synthase—ceramide synthase. (*) indicates the presence in the literature of information about resveratrol-induced changes in HepG2 cells ; (#)—in breast cancer cells ; (^)—in prostate cancer cells ; and (•)—in a mouse model of acute peritonitis .
    Figure Legend Snippet: Schematic presentation of the effects of resveratrol on sphingolipid metabolism in lung cancer A549 cells. SM—sphingomyelin; CER—ceramide; SPH—sphingosine; S1P—sphingosine-1-phosphate; nSMase—neutral sphingomyelinase; ALCER—alkaline ceramidase; SK1—sphingosine kinase 1; CER synthase—ceramide synthase. (*) indicates the presence in the literature of information about resveratrol-induced changes in HepG2 cells ; (#)—in breast cancer cells ; (^)—in prostate cancer cells ; and (•)—in a mouse model of acute peritonitis .

    Techniques Used:

    nsm activity assay asm activity  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences nsm activity assay asm activity
    TBI triggered an activation of <t>ASM</t> via posttranscriptional mechanisms. Brain tissue samples were prepared from the WT mouse brain after the injury imposed by a CCI device. Sham-injured animal brain was used as a control. A: Time course of specific ASM and <t>NSM</t> activity changes was determined. The enzyme activity is expressed as pmol of C15-SM/min per mg of protein. Data are means ± SE. * P < 0.05 (n = 10). B: Time course of ASM protein expression changes was assessed following brain trauma. Brain lysates (30 μg/lane) were analyzed by Western blotting using anti-ASM (Santa Cruz) antibody. To confirm equal loading of samples, the membranes were stripped and probed with anti-β-actin antibody. Data are representative of four independent experiments.
    Nsm Activity Assay Asm Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsm activity assay asm activity/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nsm activity assay asm activity - by Bioz Stars, 2023-12
    93/100 stars

    Images

    1) Product Images from "Acid sphingomyelinase deficiency protects mitochondria and improves function recovery after brain injury"

    Article Title: Acid sphingomyelinase deficiency protects mitochondria and improves function recovery after brain injury

    Journal: Journal of Lipid Research

    doi: 10.1194/jlr.M091132

    TBI triggered an activation of ASM via posttranscriptional mechanisms. Brain tissue samples were prepared from the WT mouse brain after the injury imposed by a CCI device. Sham-injured animal brain was used as a control. A: Time course of specific ASM and NSM activity changes was determined. The enzyme activity is expressed as pmol of C15-SM/min per mg of protein. Data are means ± SE. * P < 0.05 (n = 10). B: Time course of ASM protein expression changes was assessed following brain trauma. Brain lysates (30 μg/lane) were analyzed by Western blotting using anti-ASM (Santa Cruz) antibody. To confirm equal loading of samples, the membranes were stripped and probed with anti-β-actin antibody. Data are representative of four independent experiments.
    Figure Legend Snippet: TBI triggered an activation of ASM via posttranscriptional mechanisms. Brain tissue samples were prepared from the WT mouse brain after the injury imposed by a CCI device. Sham-injured animal brain was used as a control. A: Time course of specific ASM and NSM activity changes was determined. The enzyme activity is expressed as pmol of C15-SM/min per mg of protein. Data are means ± SE. * P < 0.05 (n = 10). B: Time course of ASM protein expression changes was assessed following brain trauma. Brain lysates (30 μg/lane) were analyzed by Western blotting using anti-ASM (Santa Cruz) antibody. To confirm equal loading of samples, the membranes were stripped and probed with anti-β-actin antibody. Data are representative of four independent experiments.

    Techniques Used: Activation Assay, Activity Assay, Expressing, Western Blot

    sphingomyelinase assay kit  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences sphingomyelinase assay kit
    Sphingomyelinase Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphingomyelinase assay kit/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphingomyelinase assay kit - by Bioz Stars, 2023-12
    93/100 stars

    Images

    nsmase2 activity  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences nsmase2 activity
    Nsmase2 Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsmase2 activity/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nsmase2 activity - by Bioz Stars, 2023-12
    93/100 stars

    Images

    neutral sphingomyelinase assay kit  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences neutral sphingomyelinase assay kit
    Neutral Sphingomyelinase Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutral sphingomyelinase assay kit/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neutral sphingomyelinase assay kit - by Bioz Stars, 2023-12
    93/100 stars

    Images

    neutral sphingomyelinase assay nsmase2 activity  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences neutral sphingomyelinase assay nsmase2 activity
    Neutral Sphingomyelinase Assay Nsmase2 Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutral sphingomyelinase assay nsmase2 activity/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neutral sphingomyelinase assay nsmase2 activity - by Bioz Stars, 2023-12
    93/100 stars

    Images

    neutral sphingomyelinase assay kit  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Echelon Biosciences neutral sphingomyelinase assay kit
    Neutral Sphingomyelinase Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutral sphingomyelinase assay kit/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neutral sphingomyelinase assay kit - by Bioz Stars, 2023-12
    93/100 stars

    Images

    k-1800  (Echelon Biosciences)


    Bioz Verified Symbol Echelon Biosciences is a verified supplier
    Bioz Manufacturer Symbol Echelon Biosciences manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Echelon Biosciences k-1800
    K 1800, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k-1800/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k-1800 - by Bioz Stars, 2023-12
    94/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Echelon Biosciences k-1800
    K 1800, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k-1800/product/Echelon Biosciences
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k-1800 - by Bioz Stars, 2023-12
    94/100 stars
      Buy from Supplier

    93
    Echelon Biosciences neutral sphingomyelinase activity assay kit
    Changes in the activity ( A ) and protein expression ( B ) of neutral <t>sphingomyelinase</t> (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.
    Neutral Sphingomyelinase Activity Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutral sphingomyelinase activity assay kit/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neutral sphingomyelinase activity assay kit - by Bioz Stars, 2023-12
    93/100 stars
      Buy from Supplier

    93
    Echelon Biosciences neutral sphingomyelinase activity
    Changes in the activity ( A ) and protein expression ( B ) of neutral <t>sphingomyelinase</t> (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.
    Neutral Sphingomyelinase Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutral sphingomyelinase activity/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neutral sphingomyelinase activity - by Bioz Stars, 2023-12
    93/100 stars
      Buy from Supplier

    93
    Echelon Biosciences nsm activity assay asm activity
    TBI triggered an activation of <t>ASM</t> via posttranscriptional mechanisms. Brain tissue samples were prepared from the WT mouse brain after the injury imposed by a CCI device. Sham-injured animal brain was used as a control. A: Time course of specific ASM and <t>NSM</t> activity changes was determined. The enzyme activity is expressed as pmol of C15-SM/min per mg of protein. Data are means ± SE. * P < 0.05 (n = 10). B: Time course of ASM protein expression changes was assessed following brain trauma. Brain lysates (30 μg/lane) were analyzed by Western blotting using anti-ASM (Santa Cruz) antibody. To confirm equal loading of samples, the membranes were stripped and probed with anti-β-actin antibody. Data are representative of four independent experiments.
    Nsm Activity Assay Asm Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsm activity assay asm activity/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nsm activity assay asm activity - by Bioz Stars, 2023-12
    93/100 stars
      Buy from Supplier

    93
    Echelon Biosciences sphingomyelinase assay kit
    TBI triggered an activation of <t>ASM</t> via posttranscriptional mechanisms. Brain tissue samples were prepared from the WT mouse brain after the injury imposed by a CCI device. Sham-injured animal brain was used as a control. A: Time course of specific ASM and <t>NSM</t> activity changes was determined. The enzyme activity is expressed as pmol of C15-SM/min per mg of protein. Data are means ± SE. * P < 0.05 (n = 10). B: Time course of ASM protein expression changes was assessed following brain trauma. Brain lysates (30 μg/lane) were analyzed by Western blotting using anti-ASM (Santa Cruz) antibody. To confirm equal loading of samples, the membranes were stripped and probed with anti-β-actin antibody. Data are representative of four independent experiments.
    Sphingomyelinase Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sphingomyelinase assay kit/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sphingomyelinase assay kit - by Bioz Stars, 2023-12
    93/100 stars
      Buy from Supplier

    93
    Echelon Biosciences nsmase2 activity
    TBI triggered an activation of <t>ASM</t> via posttranscriptional mechanisms. Brain tissue samples were prepared from the WT mouse brain after the injury imposed by a CCI device. Sham-injured animal brain was used as a control. A: Time course of specific ASM and <t>NSM</t> activity changes was determined. The enzyme activity is expressed as pmol of C15-SM/min per mg of protein. Data are means ± SE. * P < 0.05 (n = 10). B: Time course of ASM protein expression changes was assessed following brain trauma. Brain lysates (30 μg/lane) were analyzed by Western blotting using anti-ASM (Santa Cruz) antibody. To confirm equal loading of samples, the membranes were stripped and probed with anti-β-actin antibody. Data are representative of four independent experiments.
    Nsmase2 Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsmase2 activity/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nsmase2 activity - by Bioz Stars, 2023-12
    93/100 stars
      Buy from Supplier

    93
    Echelon Biosciences neutral sphingomyelinase assay kit
    TBI triggered an activation of <t>ASM</t> via posttranscriptional mechanisms. Brain tissue samples were prepared from the WT mouse brain after the injury imposed by a CCI device. Sham-injured animal brain was used as a control. A: Time course of specific ASM and <t>NSM</t> activity changes was determined. The enzyme activity is expressed as pmol of C15-SM/min per mg of protein. Data are means ± SE. * P < 0.05 (n = 10). B: Time course of ASM protein expression changes was assessed following brain trauma. Brain lysates (30 μg/lane) were analyzed by Western blotting using anti-ASM (Santa Cruz) antibody. To confirm equal loading of samples, the membranes were stripped and probed with anti-β-actin antibody. Data are representative of four independent experiments.
    Neutral Sphingomyelinase Assay Kit, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutral sphingomyelinase assay kit/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neutral sphingomyelinase assay kit - by Bioz Stars, 2023-12
    93/100 stars
      Buy from Supplier

    93
    Echelon Biosciences neutral sphingomyelinase assay nsmase2 activity
    TBI triggered an activation of <t>ASM</t> via posttranscriptional mechanisms. Brain tissue samples were prepared from the WT mouse brain after the injury imposed by a CCI device. Sham-injured animal brain was used as a control. A: Time course of specific ASM and <t>NSM</t> activity changes was determined. The enzyme activity is expressed as pmol of C15-SM/min per mg of protein. Data are means ± SE. * P < 0.05 (n = 10). B: Time course of ASM protein expression changes was assessed following brain trauma. Brain lysates (30 μg/lane) were analyzed by Western blotting using anti-ASM (Santa Cruz) antibody. To confirm equal loading of samples, the membranes were stripped and probed with anti-β-actin antibody. Data are representative of four independent experiments.
    Neutral Sphingomyelinase Assay Nsmase2 Activity, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutral sphingomyelinase assay nsmase2 activity/product/Echelon Biosciences
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neutral sphingomyelinase assay nsmase2 activity - by Bioz Stars, 2023-12
    93/100 stars
      Buy from Supplier

    Image Search Results


    Changes in the activity ( A ) and protein expression ( B ) of neutral sphingomyelinase (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: Effect of Quercetin and Fingolimod, Alone or in Combination, on the Sphingolipid Metabolism in HepG2 Cells

    doi: 10.3390/ijms232213916

    Figure Lengend Snippet: Changes in the activity ( A ) and protein expression ( B ) of neutral sphingomyelinase (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.

    Article Snippet: Neutral sphingomyelinase activity was measured by the Neutral Sphingomyelinase Activity assay Kit (Echelon Biosciences, Cat.# K-1800) (Salt Lake City, UT, USA).

    Techniques: Activity Assay, Expressing, Western Blot

    Changes in the activity ( A ) and protein expression ( B ) of neutral sphingomyelinase (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: Effect of Quercetin and Fingolimod, Alone or in Combination, on the Sphingolipid Metabolism in HepG2 Cells

    doi: 10.3390/ijms232213916

    Figure Lengend Snippet: Changes in the activity ( A ) and protein expression ( B ) of neutral sphingomyelinase (nSMase) in control and treated with quercetin (Q) and fingolimod (F) HepG2 cells. Values are expressed as % of controls (100%). Representative images from Western blot analysis with specific antibodies to neutral sphingomyelinase (anti-nSMase) are shown at the lowermost of panel B. Reaction with anti-glyceraldehyde-3-phosphate dehydrogenase antibodies (anti-GAPDH) was used as an internal control for loading. Values are means ± SD. Statistical significance is presented as comparison of treated cells with controls. * p < 0.05; ** p < 0.01.

    Article Snippet: Neutral sphingomyelinase activity was measured by the Neutral Sphingomyelinase Activity assay Kit (Echelon Biosciences, Cat.# K-1800) (Salt Lake City, UT, USA).

    Techniques: Activity Assay, Expressing, Western Blot

    TBI triggered an activation of ASM via posttranscriptional mechanisms. Brain tissue samples were prepared from the WT mouse brain after the injury imposed by a CCI device. Sham-injured animal brain was used as a control. A: Time course of specific ASM and NSM activity changes was determined. The enzyme activity is expressed as pmol of C15-SM/min per mg of protein. Data are means ± SE. * P < 0.05 (n = 10). B: Time course of ASM protein expression changes was assessed following brain trauma. Brain lysates (30 μg/lane) were analyzed by Western blotting using anti-ASM (Santa Cruz) antibody. To confirm equal loading of samples, the membranes were stripped and probed with anti-β-actin antibody. Data are representative of four independent experiments.

    Journal: Journal of Lipid Research

    Article Title: Acid sphingomyelinase deficiency protects mitochondria and improves function recovery after brain injury

    doi: 10.1194/jlr.M091132

    Figure Lengend Snippet: TBI triggered an activation of ASM via posttranscriptional mechanisms. Brain tissue samples were prepared from the WT mouse brain after the injury imposed by a CCI device. Sham-injured animal brain was used as a control. A: Time course of specific ASM and NSM activity changes was determined. The enzyme activity is expressed as pmol of C15-SM/min per mg of protein. Data are means ± SE. * P < 0.05 (n = 10). B: Time course of ASM protein expression changes was assessed following brain trauma. Brain lysates (30 μg/lane) were analyzed by Western blotting using anti-ASM (Santa Cruz) antibody. To confirm equal loading of samples, the membranes were stripped and probed with anti-β-actin antibody. Data are representative of four independent experiments.

    Article Snippet: ASM and NSM activity assay ASM activity was measured using an ASM activity assay kit (Echelon Biosciences, Salt Lake City, UT) according to the manufacturer’s instructions.

    Techniques: Activation Assay, Activity Assay, Expressing, Western Blot