k pneumonia atcc 10031  (ATCC)


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    • 99
    Name:
    Klebsiella pneumoniae subsp pneumoniae
    Description:

    Catalog Number:
    10031-MINI-PACK
    Price:
    None
    Applications:
    ATCC® 10031-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 10031™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.  Learn more about ATCC® MinisDeveloped by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures®. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go! . Assay of capreomycin sulfate capreomycin, capromycin Assay of cefamandole Assay of cefoxitin Assay of cephaloglycin Assay of cephaloridine cephalomycin Assay of cephalothin Assay of dihydrostreptomycin sulfate dihydrostreptomycin Assay of erythromycin Assay of neomycin Assay of spectinomycin actinospectacin Respiratory research Pharmaceutical and Personal Care Assay of streptomycin Assay of troleandomycin Inhibition testing Quality control strain Quality control strain for Sensititre products
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    ATCC k pneumonia atcc 10031

    https://www.bioz.com/result/k pneumonia atcc 10031/product/ATCC
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    k pneumonia atcc 10031 - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Rapid Evolution of Reduced Susceptibility against a Balanced Dual-Targeting Antibiotic through Stepping-Stone Mutations
    Article Snippet: .. Following adaptive laboratory evolution of ciprofloxacin resistance in K. pneumoniae ATCC 10031 and E. coli K-12 MG1655, single colonies from three adapted lines were subjected to whole-genome sequencing on an Illumina HiSeq 4000 platform. .. Prior to sequencing, genomic DNA (gDNA) was isolated from each adapted line and the corresponding wild-type strain by using the GenElute gDNA isolation kit (Sigma-Aldrich), according to the manufacturer’s instructions.

    Isolation:

    Article Title: Chromatographic Characterization and GC-MS Evaluation of the Bioactive Constituents with Antimicrobial Potential from the Pigmented Ink of Loligo duvauceli
    Article Snippet: .. Antimicrobial Bioassay The antimicrobial activity of isolated fractions was checked against Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 10031), Staphylococcus aureus (ATCC 25923), Candida albicans (ATCC 10231), and Lactobacillus acidophilus [MTCC 447]. ..

    other:

    Article Title: Genome-guided Investigation of Antibiotic Substances produced by Allosalinactinospora lopnorensis CA15-2T from Lop Nor region, China
    Article Snippet: Eight standard strains including Acinetobacter baumannii ATCC 19606, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 10031 and ATCC 700603, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 33186 and ATCC 29212, Pseudomonas aeruginosa ATCC 27853 were obtained from American Type Culture Collection (ATCC, Rockville, MD).

    Activity Assay:

    Article Title: Chromatographic Characterization and GC-MS Evaluation of the Bioactive Constituents with Antimicrobial Potential from the Pigmented Ink of Loligo duvauceli
    Article Snippet: .. Antimicrobial Bioassay The antimicrobial activity of isolated fractions was checked against Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 10031), Staphylococcus aureus (ATCC 25923), Candida albicans (ATCC 10231), and Lactobacillus acidophilus [MTCC 447]. ..

    Concentration Assay:

    Article Title: Rapid Evolution of Reduced Susceptibility against a Balanced Dual-Targeting Antibiotic through Stepping-Stone Mutations
    Article Snippet: .. Briefly, heat-induced pORTMAGE3 (Addgene no. 72678) containing Klebsiella pneumoniae ATCC 10031 cells was electroporated with KpLacZW23* oligonucleotide (2.5μM final concentration). .. Following oligonucleotide integration into the bacterial chromosome, lacZ -deficient variants were identified by plating cells out to X-Gal plus IPTG-containing MHBII agar plates at appropriate dilution to form single colonies, where knockout mutants formed white colonies and could be easily distinguished from the dark-blue colonies of the wild type containing a functional β-galactosidase gene.

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    • k pneumoniae atcc 10031  (ATCC)
    99
    ATCC k pneumoniae atcc 10031
    Adaptive laboratory evolution of Klebsiella <t>pneumoniae</t> and Escherichia coli under ciprofloxacin stress. (A) Antibiotic concentrations at which K. pneumoniae <t>ATCC</t> 10031 as well as E. coli K-12 MG1655 wild-type and Δ mutS ). (B) Relative resistance level of the evolved lines against ciprofloxacin (gray bars) and gepotidacin (yellow bars) compared to the wild type at the end of the adaptive laboratory evolution. Asterisk (*) indicates that the evolved line was subjected to whole-genome sequence analysis to uncover mutational processes behind the reduced susceptibility.
    K Pneumoniae Atcc 10031, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k pneumoniae atcc 10031/product/ATCC
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    k pneumoniae atcc 10031 - by Bioz Stars, 2021-01
    99/100 stars
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    Adaptive laboratory evolution of Klebsiella pneumoniae and Escherichia coli under ciprofloxacin stress. (A) Antibiotic concentrations at which K. pneumoniae ATCC 10031 as well as E. coli K-12 MG1655 wild-type and Δ mutS ). (B) Relative resistance level of the evolved lines against ciprofloxacin (gray bars) and gepotidacin (yellow bars) compared to the wild type at the end of the adaptive laboratory evolution. Asterisk (*) indicates that the evolved line was subjected to whole-genome sequence analysis to uncover mutational processes behind the reduced susceptibility.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Rapid Evolution of Reduced Susceptibility against a Balanced Dual-Targeting Antibiotic through Stepping-Stone Mutations

    doi: 10.1128/AAC.00207-19

    Figure Lengend Snippet: Adaptive laboratory evolution of Klebsiella pneumoniae and Escherichia coli under ciprofloxacin stress. (A) Antibiotic concentrations at which K. pneumoniae ATCC 10031 as well as E. coli K-12 MG1655 wild-type and Δ mutS ). (B) Relative resistance level of the evolved lines against ciprofloxacin (gray bars) and gepotidacin (yellow bars) compared to the wild type at the end of the adaptive laboratory evolution. Asterisk (*) indicates that the evolved line was subjected to whole-genome sequence analysis to uncover mutational processes behind the reduced susceptibility.

    Article Snippet: Specifically, we examined wound colonization for representative gepotidacin- and ciprofloxacin-resistant mutants of K. pneumoniae ATCC 10031 and compared it to that of the wild type.

    Techniques: Sequencing

    Fitness cost and virulence of bacteria with reduced susceptibility to gepotidacin. (A) In vitro competition between mutant and wild-type Klebsiella pneumoniae . Isogenic mutants of Klebsiella pneumoniae ATCC 10031 carrying either a mutation combination conferring reduced susceptibility to gepotidacin or its single-step constituents (red), or clinically occurring fluoroquinolone resistance-associated mutations (blue) competed against the wild-type strain. In competition assays, a competitive index of

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Rapid Evolution of Reduced Susceptibility against a Balanced Dual-Targeting Antibiotic through Stepping-Stone Mutations

    doi: 10.1128/AAC.00207-19

    Figure Lengend Snippet: Fitness cost and virulence of bacteria with reduced susceptibility to gepotidacin. (A) In vitro competition between mutant and wild-type Klebsiella pneumoniae . Isogenic mutants of Klebsiella pneumoniae ATCC 10031 carrying either a mutation combination conferring reduced susceptibility to gepotidacin or its single-step constituents (red), or clinically occurring fluoroquinolone resistance-associated mutations (blue) competed against the wild-type strain. In competition assays, a competitive index of

    Article Snippet: Specifically, we examined wound colonization for representative gepotidacin- and ciprofloxacin-resistant mutants of K. pneumoniae ATCC 10031 and compared it to that of the wild type.

    Techniques: In Vitro, Mutagenesis

    Coomassie-stained SDS-PAGE (10%) protein profiles of K. pneumoniae ATCC 10031, MS K. pneumoniae and KPC-producing K. pneumoniae and relative OMVs. Molecular mass marker (MW) is expressed in kilodaltons (kDa). The rectangles indicate the bands subjected to trypsin digestion and MS and MS/MS analysis.

    Journal: Microorganisms

    Article Title: Outer Membrane Vesicles Derived from Klebsiella pneumoniae Influence the miRNA Expression Profile in Human Bronchial Epithelial BEAS-2B Cells

    doi: 10.3390/microorganisms8121985

    Figure Lengend Snippet: Coomassie-stained SDS-PAGE (10%) protein profiles of K. pneumoniae ATCC 10031, MS K. pneumoniae and KPC-producing K. pneumoniae and relative OMVs. Molecular mass marker (MW) is expressed in kilodaltons (kDa). The rectangles indicate the bands subjected to trypsin digestion and MS and MS/MS analysis.

    Article Snippet: Vesicle proteins from K. pneumoniae ATCC 10031, MS K. pneumoniae and KPC-producing K. pneumoniae were analysed by 10% SDS-PAGE.

    Techniques: Staining, SDS Page, Marker, Tandem Mass Spectroscopy

    Validation of miRNA microarray data by real-time PCR. The expression levels of miR-21, miR-25, miR223, and let-7g were consistent with the miRNA microarray results after treatment with K. pneumoniae ATCC 10031 ( A ), MS K. pneumoniae ( B ) and KPC-producing K. pneumoniae ( C ).

    Journal: Microorganisms

    Article Title: Outer Membrane Vesicles Derived from Klebsiella pneumoniae Influence the miRNA Expression Profile in Human Bronchial Epithelial BEAS-2B Cells

    doi: 10.3390/microorganisms8121985

    Figure Lengend Snippet: Validation of miRNA microarray data by real-time PCR. The expression levels of miR-21, miR-25, miR223, and let-7g were consistent with the miRNA microarray results after treatment with K. pneumoniae ATCC 10031 ( A ), MS K. pneumoniae ( B ) and KPC-producing K. pneumoniae ( C ).

    Article Snippet: Vesicle proteins from K. pneumoniae ATCC 10031, MS K. pneumoniae and KPC-producing K. pneumoniae were analysed by 10% SDS-PAGE.

    Techniques: Microarray, Real-time Polymerase Chain Reaction, Expressing

    Gene prediction analysis. MiRNA sequences up and downregulated in the BEAS-2B sample exposed to OMVs of K. pneumoniae ATCC 10031 ( A , B ), MS K. pneumoniae ( C , D ) and KPC-producing K. pneumoniae ( E , F ).

    Journal: Microorganisms

    Article Title: Outer Membrane Vesicles Derived from Klebsiella pneumoniae Influence the miRNA Expression Profile in Human Bronchial Epithelial BEAS-2B Cells

    doi: 10.3390/microorganisms8121985

    Figure Lengend Snippet: Gene prediction analysis. MiRNA sequences up and downregulated in the BEAS-2B sample exposed to OMVs of K. pneumoniae ATCC 10031 ( A , B ), MS K. pneumoniae ( C , D ) and KPC-producing K. pneumoniae ( E , F ).

    Article Snippet: Vesicle proteins from K. pneumoniae ATCC 10031, MS K. pneumoniae and KPC-producing K. pneumoniae were analysed by 10% SDS-PAGE.

    Techniques: