Structured Review

Thermo Fisher k atpase
STED microscopy dissecting the localization of Na + ,K + <t>-ATPase</t> in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled <t>α3</t> NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm
K Atpase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/k atpase/product/Thermo Fisher
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
k atpase - by Bioz Stars, 2020-05
92/100 stars

Images

1) Product Images from "Spatial distribution of Na+-K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy"

Article Title: Spatial distribution of Na+-K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy

Journal: BMC Neuroscience

doi: 10.1186/1471-2202-12-16

STED microscopy dissecting the localization of Na + ,K + -ATPase in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled α3 NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm
Figure Legend Snippet: STED microscopy dissecting the localization of Na + ,K + -ATPase in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled α3 NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm

Techniques Used: Microscopy, Cell Culture

2) Product Images from "Nitric oxide synthase in cardiac sarcoplasmic reticulum"

Article Title: Nitric oxide synthase in cardiac sarcoplasmic reticulum

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

( a ) Immunofluorescent localization of cardiomyocyte antigens with mAbs on cryostat sections of human ventricular myocardium by laser-scanning confocal microscopy. ( aA ) nNOS indirect immunofluorescent labeling (IIL) shows a linear longitudinal and transverse, striated pattern of labeling; Na + ,K + -ATPase IIL demonstrates a distinctly different sarcolemma pattern of labeling of the human heart section ( aB ); RyR IIL ( aC ) and SERCA2 IIL ( aD ) have a similar pattern of labeling as NOS IIL suggesting SR localization of NOS rather than exclusive sarcolemma localization in cardiac muscle; an irrelevant anti-viral protein mAb ( aE ) used at the same concentration as the other primary mAbs, has only low levels of background fluorescence. ( b ) Single immunogold labeling. SR vesicles were incubated with specific primary antibodies and a secondary antibody conjugated to 12 nm of colloidalgold. Control vesicles in the absence of primary antibody ( bA ); with anti-eNOS ( bB ); with anti-iNOS ( bC ); and single vesicle ( bD ) and a group of vesicles ( bE ) with anti-nNOS. Electron photomicrographs show that only nNOS is broadly dispersed on the surface of cardiac muscle SR membrane vesicles. Double immunogold labeling: Ca 2+ -ATPase (6 nm gold particles) and nNOS (12 nm) ( bF ); Na + , K + -ATPase (6 nm) and nNOS (12 nm) ( bG ); and phospholamban (6 nm) and nNOS (12 nm) ( bH ). Each vesicle represents hundreds of similar labeled or unlabeled vesicles in each condition. The results show that cardiac SR Ca 2+ -ATPase and phospholamban coexist with a SR neuronal-type NOS. Absence of Na + ,K + -ATPase on the SR vesicles further demonstrates the vesicles represent SR rather than sarcolemma.
Figure Legend Snippet: ( a ) Immunofluorescent localization of cardiomyocyte antigens with mAbs on cryostat sections of human ventricular myocardium by laser-scanning confocal microscopy. ( aA ) nNOS indirect immunofluorescent labeling (IIL) shows a linear longitudinal and transverse, striated pattern of labeling; Na + ,K + -ATPase IIL demonstrates a distinctly different sarcolemma pattern of labeling of the human heart section ( aB ); RyR IIL ( aC ) and SERCA2 IIL ( aD ) have a similar pattern of labeling as NOS IIL suggesting SR localization of NOS rather than exclusive sarcolemma localization in cardiac muscle; an irrelevant anti-viral protein mAb ( aE ) used at the same concentration as the other primary mAbs, has only low levels of background fluorescence. ( b ) Single immunogold labeling. SR vesicles were incubated with specific primary antibodies and a secondary antibody conjugated to 12 nm of colloidalgold. Control vesicles in the absence of primary antibody ( bA ); with anti-eNOS ( bB ); with anti-iNOS ( bC ); and single vesicle ( bD ) and a group of vesicles ( bE ) with anti-nNOS. Electron photomicrographs show that only nNOS is broadly dispersed on the surface of cardiac muscle SR membrane vesicles. Double immunogold labeling: Ca 2+ -ATPase (6 nm gold particles) and nNOS (12 nm) ( bF ); Na + , K + -ATPase (6 nm) and nNOS (12 nm) ( bG ); and phospholamban (6 nm) and nNOS (12 nm) ( bH ). Each vesicle represents hundreds of similar labeled or unlabeled vesicles in each condition. The results show that cardiac SR Ca 2+ -ATPase and phospholamban coexist with a SR neuronal-type NOS. Absence of Na + ,K + -ATPase on the SR vesicles further demonstrates the vesicles represent SR rather than sarcolemma.

Techniques Used: Confocal Microscopy, Labeling, Concentration Assay, Fluorescence, Incubation

3) Product Images from "Nitric oxide synthase in cardiac sarcoplasmic reticulum"

Article Title: Nitric oxide synthase in cardiac sarcoplasmic reticulum

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

( a ) Immunofluorescent localization of cardiomyocyte antigens with mAbs on cryostat sections of human ventricular myocardium by laser-scanning confocal microscopy. ( aA ) nNOS indirect immunofluorescent labeling (IIL) shows a linear longitudinal and transverse, striated pattern of labeling; Na + ,K + -ATPase IIL demonstrates a distinctly different sarcolemma pattern of labeling of the human heart section ( aB ); RyR IIL ( aC ) and SERCA2 IIL ( aD ) have a similar pattern of labeling as NOS IIL suggesting SR localization of NOS rather than exclusive sarcolemma localization in cardiac muscle; an irrelevant anti-viral protein mAb ( aE ) used at the same concentration as the other primary mAbs, has only low levels of background fluorescence. ( b ) Single immunogold labeling. SR vesicles were incubated with specific primary antibodies and a secondary antibody conjugated to 12 nm of colloidalgold. Control vesicles in the absence of primary antibody ( bA ); with anti-eNOS ( bB ); with anti-iNOS ( bC ); and single vesicle ( bD ) and a group of vesicles ( bE ) with anti-nNOS. Electron photomicrographs show that only nNOS is broadly dispersed on the surface of cardiac muscle SR membrane vesicles. Double immunogold labeling: Ca 2+ -ATPase (6 nm gold particles) and nNOS (12 nm) ( bF ); Na + , K + -ATPase (6 nm) and nNOS (12 nm) ( bG ); and phospholamban (6 nm) and nNOS (12 nm) ( bH ). Each vesicle represents hundreds of similar labeled or unlabeled vesicles in each condition. The results show that cardiac SR Ca 2+ -ATPase and phospholamban coexist with a SR neuronal-type NOS. Absence of Na + ,K + -ATPase on the SR vesicles further demonstrates the vesicles represent SR rather than sarcolemma.
Figure Legend Snippet: ( a ) Immunofluorescent localization of cardiomyocyte antigens with mAbs on cryostat sections of human ventricular myocardium by laser-scanning confocal microscopy. ( aA ) nNOS indirect immunofluorescent labeling (IIL) shows a linear longitudinal and transverse, striated pattern of labeling; Na + ,K + -ATPase IIL demonstrates a distinctly different sarcolemma pattern of labeling of the human heart section ( aB ); RyR IIL ( aC ) and SERCA2 IIL ( aD ) have a similar pattern of labeling as NOS IIL suggesting SR localization of NOS rather than exclusive sarcolemma localization in cardiac muscle; an irrelevant anti-viral protein mAb ( aE ) used at the same concentration as the other primary mAbs, has only low levels of background fluorescence. ( b ) Single immunogold labeling. SR vesicles were incubated with specific primary antibodies and a secondary antibody conjugated to 12 nm of colloidalgold. Control vesicles in the absence of primary antibody ( bA ); with anti-eNOS ( bB ); with anti-iNOS ( bC ); and single vesicle ( bD ) and a group of vesicles ( bE ) with anti-nNOS. Electron photomicrographs show that only nNOS is broadly dispersed on the surface of cardiac muscle SR membrane vesicles. Double immunogold labeling: Ca 2+ -ATPase (6 nm gold particles) and nNOS (12 nm) ( bF ); Na + , K + -ATPase (6 nm) and nNOS (12 nm) ( bG ); and phospholamban (6 nm) and nNOS (12 nm) ( bH ). Each vesicle represents hundreds of similar labeled or unlabeled vesicles in each condition. The results show that cardiac SR Ca 2+ -ATPase and phospholamban coexist with a SR neuronal-type NOS. Absence of Na + ,K + -ATPase on the SR vesicles further demonstrates the vesicles represent SR rather than sarcolemma.

Techniques Used: Confocal Microscopy, Labeling, Concentration Assay, Fluorescence, Incubation

Related Articles

Transduction:

Article Title: Nitric oxide synthase in cardiac sarcoplasmic reticulum
Article Snippet: .. Primary mAbs to cardiac SR Ca2+ -ATPase (SERCA2, Affinity BioReagents), RyR (MA3–925, Affinity BioReagents), Na+ ,K+ -ATPase (Affinity BioReagents), nNOS ( , Transduction Laboratories, Lexington, KY) and simian immunodeficiency virus Gag (Karen Kent, AIDS Research and Reference Reagents) were used at 1:100. .. Donkey anti-mouse antibodies labeled with Cy3 (Jackson Immuno Research) were used at 1:500 as secondary antibodies.

Cell Attachment Assay:

Article Title: Ouabain modulates ciliogenesis in epithelial cells
Article Snippet: .. Contreras RG, Shoshani L, Flores-Maldonado C, Lázaro A, Cereijido M. Relationship between Na(+),K(+)-ATPase and cell attachment. ..

Article Title: Ouabain modulates ciliogenesis in epithelial cells
Article Snippet: .. Contreras RG, et al. Ouabain binding to Na+,K+-ATPase relaxes cell attachment and sends a specific signal (NACos) to the nucleus. ..

Labeling:

Article Title: Nitric oxide synthase in cardiac sarcoplasmic reticulum
Article Snippet: .. Immunogold labeling ( ) was performed by adsorbing SR vesicles (0.04 μg/ml) on copper grids and incubating the grids with the primary antibodies [anti-eNOS (PA1–037), anti-iNOS (PA1–036), and anti-nNOS (PA3–032) for the single labeling; anti-SERCA2 (MA3–910), anti-Na+ ,K+ -ATPase (MA3–928), and anti-phospholamban (MA3–922) for the double labeling, (Affinity BioReagents)] diluted 1:250 in PBS. .. After 30 min of incubation at room temperature, the grids were washed with PBS and then incubated for 30 min with a secondary antibody (donkey anti-mouse IgG or anti-rabbit IgG from Jackson ImmnoResearch) conjugated to 12 nm of colloidal gold diluted 1:40 in PBS.

other:

Article Title: Ouabain modulates ciliogenesis in epithelial cells
Article Snippet: Menco BP, et al. Ultrastructural localization of amiloride-sensitive sodium channels and Na+,K(+)-ATPase in the rat's olfactory epithelial surface.

Expressing:

Article Title: Ouabain modulates ciliogenesis in epithelial cells
Article Snippet: .. The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells. ..

Affinity Purification:

Article Title: Detergent-Resistant Membrane Microdomains Facilitate Ib Oligomer Formation and Biological Activity of Clostridium perfringens Iota-Toxin
Article Snippet: .. Mouse monoclonal antibodies against clathrin heavy chain and α-1 Na+ ,K+ -ATPase as well as affinity-purified rabbit antibody against dynamin II were purchased from Affinity BioReagents (Golden, Colo.). .. Affinity-purified antibodies against flotillin-1 and caveolin-1 were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, Calif.).

Binding Assay:

Article Title: Ouabain modulates ciliogenesis in epithelial cells
Article Snippet: .. Contreras RG, et al. Ouabain binding to Na+,K+-ATPase relaxes cell attachment and sends a specific signal (NACos) to the nucleus. ..

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  • 91
    Thermo Fisher anti na k atpase
    Cu-deficiency stimulates the degradation of ATP7A protein. (a) IEC-6 cells pretreated with media containing 100 μM CuCl 2 for 2.5 h were further treated with 50 μg/mL cycloheximide (CHX). Following 30 min incubation, cells were exposed to fresh culture media containing 300 μM BCS and 50 μg/ml CHX and then lysed after 0.5, 1, 3, or 6 h (lanes 3–6). Whole cell lysates were processed for immunoblotting analysis using antibodies as indicated. Immunoblot images are representative results of four independent experiments. (b) IEC-6 cells were pretreated with media containing 100 μM CuCl 2 for 1 h before addition of 20 μM MG132, 100 nM bortezomib, or DMSO (vehicle control) and treated for another 90 min. Cells were further treated with 50 μg/mL CHX for 30 min, and then the media was replaced with fresh media containing 300 μM BCS and 50 μg/mL CHX for 2 h in the presence of MG132 and/or bortezomib, or DMSO. Na + /K + <t>-ATPase</t> served as a loading control. Representative immunoblots of four independent experiments are shown. Full-length blots are presented in Supplementary Figure 12 .
    Anti Na K Atpase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti na k atpase/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti na k atpase - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    89
    Thermo Fisher mouse anti na k atpase
    TRPM1 is present on the plasma membrane of mGluR6-null ON bipolar cell's processes. Shown are dissociated cells that retained their processes; staining for Na + -K + <t>-ATPase</t> is in cyan, for TRPM in red, and DAPI stain is blue. A1 : low magnification of a WT
    Mouse Anti Na K Atpase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti na k atpase/product/Thermo Fisher
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti na k atpase - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    91
    Thermo Fisher h k atpase
    Immunohistochemical detection of Troy + cells in the gastric corpus mucosa A-D . Whole section overview of the stomach epithelium. As expected, nuclei were immunonegative (original magnification A: 60x, inserts B-D: 250x). E-I . Identification of Troy + cells (brown DAB staining) by double staining with lineage markers (VectorRed): gastric H + /K + <t>-ATPase</t> (E), pepsinogen C (F), chromogranin A (G), Muc6 (H) or Muc5 (I); original magnifications 400x. K . Troy + myocytes allowed for internal staining control.
    H K Atpase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h k atpase/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h k atpase - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    Image Search Results


    Cu-deficiency stimulates the degradation of ATP7A protein. (a) IEC-6 cells pretreated with media containing 100 μM CuCl 2 for 2.5 h were further treated with 50 μg/mL cycloheximide (CHX). Following 30 min incubation, cells were exposed to fresh culture media containing 300 μM BCS and 50 μg/ml CHX and then lysed after 0.5, 1, 3, or 6 h (lanes 3–6). Whole cell lysates were processed for immunoblotting analysis using antibodies as indicated. Immunoblot images are representative results of four independent experiments. (b) IEC-6 cells were pretreated with media containing 100 μM CuCl 2 for 1 h before addition of 20 μM MG132, 100 nM bortezomib, or DMSO (vehicle control) and treated for another 90 min. Cells were further treated with 50 μg/mL CHX for 30 min, and then the media was replaced with fresh media containing 300 μM BCS and 50 μg/mL CHX for 2 h in the presence of MG132 and/or bortezomib, or DMSO. Na + /K + -ATPase served as a loading control. Representative immunoblots of four independent experiments are shown. Full-length blots are presented in Supplementary Figure 12 .

    Journal: Scientific Reports

    Article Title: Organ-specific regulation of ATP7A abundance is coordinated with systemic copper homeostasis

    doi: 10.1038/s41598-017-11961-z

    Figure Lengend Snippet: Cu-deficiency stimulates the degradation of ATP7A protein. (a) IEC-6 cells pretreated with media containing 100 μM CuCl 2 for 2.5 h were further treated with 50 μg/mL cycloheximide (CHX). Following 30 min incubation, cells were exposed to fresh culture media containing 300 μM BCS and 50 μg/ml CHX and then lysed after 0.5, 1, 3, or 6 h (lanes 3–6). Whole cell lysates were processed for immunoblotting analysis using antibodies as indicated. Immunoblot images are representative results of four independent experiments. (b) IEC-6 cells were pretreated with media containing 100 μM CuCl 2 for 1 h before addition of 20 μM MG132, 100 nM bortezomib, or DMSO (vehicle control) and treated for another 90 min. Cells were further treated with 50 μg/mL CHX for 30 min, and then the media was replaced with fresh media containing 300 μM BCS and 50 μg/mL CHX for 2 h in the presence of MG132 and/or bortezomib, or DMSO. Na + /K + -ATPase served as a loading control. Representative immunoblots of four independent experiments are shown. Full-length blots are presented in Supplementary Figure 12 .

    Article Snippet: Antibodies The anti-ATP7A antibody (generous gift from Drs. Stephen G. Kaler, National Institutes of Health (NIH), Bethesda, MD and Michael J. Petris, University of Missouri, Columbia, MO), anti-ATP7B antibody (generous gift from Dr. Svetlana Lutsenko, Johns Hopkins University, Baltimore, MD), anti-Ctr1 antibody , anti-β-tubulin antibody (Cell Signaling Technology), anti-β-actin antibody (Sigma), and anti-Na+ /K+ -ATPase (Thermo Scientific) antibody were each used at a 1:1,000 dilution.

    Techniques: Incubation, Western Blot

    TRPM1 is present on the plasma membrane of mGluR6-null ON bipolar cell's processes. Shown are dissociated cells that retained their processes; staining for Na + -K + -ATPase is in cyan, for TRPM in red, and DAPI stain is blue. A1 : low magnification of a WT

    Journal: Journal of Neurophysiology

    Article Title: mGluR6 deletion renders the TRPM1 channel in retina inactive

    doi: 10.1152/jn.00933.2011

    Figure Lengend Snippet: TRPM1 is present on the plasma membrane of mGluR6-null ON bipolar cell's processes. Shown are dissociated cells that retained their processes; staining for Na + -K + -ATPase is in cyan, for TRPM in red, and DAPI stain is blue. A1 : low magnification of a WT

    Article Snippet: The following antibodies were used: rabbit anti-Gαo , 1:100 (MAB 3073, Millipore, Billerica, MA); rabbit anti-Gβ3 , 1:300 (HPA005645, Sigma, St. Louis, MO); rabbit anti-Gγ13 , 1:500 (gift of Dr. Robert Margolskee); rabbit anti-TRPM1, 1:100 (prepared by Dr. Furukawa's group, for immunocytochemistry); sheep anti-TRPM1, 1:1,1000 (Gift of Dr. Kirill Martemayanov, for Western blots); rabbit anti-Gβ5 , 1:500 (gift of Theodore Wensel); rabbit anti-RGS11, 1:1000 (gift of Theodore Wensel); mouse anti-Na+ -K+ -ATPase, 1:100 (MA3–915, Thermo Scientific, Rockford, IL); and rabbit anti-ERGIC-53/58 (p58), 1:1000 (Sigma-Aldrich).

    Techniques: Staining

    Immunohistochemical detection of Troy + cells in the gastric corpus mucosa A-D . Whole section overview of the stomach epithelium. As expected, nuclei were immunonegative (original magnification A: 60x, inserts B-D: 250x). E-I . Identification of Troy + cells (brown DAB staining) by double staining with lineage markers (VectorRed): gastric H + /K + -ATPase (E), pepsinogen C (F), chromogranin A (G), Muc6 (H) or Muc5 (I); original magnifications 400x. K . Troy + myocytes allowed for internal staining control.

    Journal: Oncotarget

    Article Title: Troy is expressed in human stomach mucosa and a novel putative prognostic marker of intestinal type gastric cancer

    doi: 10.18632/oncotarget.10672

    Figure Lengend Snippet: Immunohistochemical detection of Troy + cells in the gastric corpus mucosa A-D . Whole section overview of the stomach epithelium. As expected, nuclei were immunonegative (original magnification A: 60x, inserts B-D: 250x). E-I . Identification of Troy + cells (brown DAB staining) by double staining with lineage markers (VectorRed): gastric H + /K + -ATPase (E), pepsinogen C (F), chromogranin A (G), Muc6 (H) or Muc5 (I); original magnifications 400x. K . Troy + myocytes allowed for internal staining control.

    Article Snippet: For double staining, the following antibodies were used: H+ /K+ -ATPase (1:2000, 2G11, Thermo Scientific), pepsinogen C (1:100, ABIN1999516, antibodies-online, Aachen, Germany), Muc5AC (1:100), Muc6 (1:40), chromogranin A (1:100, all Leica Biosystems, Nussloch, Germany).

    Techniques: Immunohistochemistry, Staining, Double Staining