Structured Review

Millipore k atpase
Effect of vanadate on renal <t>α</t> 1 -Na, <t>K-ATPase</t> protein abundance. Ten days of vanadate injection significantly suppressed α 1 -Na, K-ATPase protein abundance. Histogram bars show the densitometric analyses ratios of α 1 -Na, K-ATPase to β-actin intensity, and the representative immunoblot photographs are present. All values are expressed as the mean ± SD of 8 rats/group. *Significantly different from the sham group ( p
K Atpase, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Vanadate-Induced Renal cAMP and Malondialdehyde Accumulation Suppresses Alpha 1 Sodium Potassium Adenosine Triphosphatase Protein Levels"

Article Title: Vanadate-Induced Renal cAMP and Malondialdehyde Accumulation Suppresses Alpha 1 Sodium Potassium Adenosine Triphosphatase Protein Levels

Journal: Toxicological Research

doi: 10.5487/TR.2018.34.2.143

Effect of vanadate on renal α 1 -Na, K-ATPase protein abundance. Ten days of vanadate injection significantly suppressed α 1 -Na, K-ATPase protein abundance. Histogram bars show the densitometric analyses ratios of α 1 -Na, K-ATPase to β-actin intensity, and the representative immunoblot photographs are present. All values are expressed as the mean ± SD of 8 rats/group. *Significantly different from the sham group ( p
Figure Legend Snippet: Effect of vanadate on renal α 1 -Na, K-ATPase protein abundance. Ten days of vanadate injection significantly suppressed α 1 -Na, K-ATPase protein abundance. Histogram bars show the densitometric analyses ratios of α 1 -Na, K-ATPase to β-actin intensity, and the representative immunoblot photographs are present. All values are expressed as the mean ± SD of 8 rats/group. *Significantly different from the sham group ( p

Techniques Used: Injection

Representative immunohistochemical staining images of renal α 1 -Na, K-ATPase protein localization in the cortex (A, D), outer medulla (B, E), and inner medulla (C, F) from sham (A–C), and vanadate (D–F) (n = 5/group). Vanadate reduced immunoreactivity both in the cortex (PCT and Pcap; arrows) and the medulla (PTs and MCD; arrow heads). Bar scale = 20 μm.
Figure Legend Snippet: Representative immunohistochemical staining images of renal α 1 -Na, K-ATPase protein localization in the cortex (A, D), outer medulla (B, E), and inner medulla (C, F) from sham (A–C), and vanadate (D–F) (n = 5/group). Vanadate reduced immunoreactivity both in the cortex (PCT and Pcap; arrows) and the medulla (PTs and MCD; arrow heads). Bar scale = 20 μm.

Techniques Used: Immunohistochemistry, Staining

2) Product Images from "Regulation of cerebrospinal fluid production by caffeine consumption"

Article Title: Regulation of cerebrospinal fluid production by caffeine consumption

Journal: BMC Neuroscience

doi: 10.1186/1471-2202-10-110

The effect inversion of caffeine was associated with the induction of the A 1 adenosine receptor after long-term caffeine treatment . A . Representative photographs of adenosine A 1 and A 2A receptor immunohistochemistry in the choroid epithelial cells of the control and caffeine-treated rats (n = 4). Scale bar, 100 μm. B . Representative Western blots of the adenosine A 1 and A 2 receptors. C . Analysis of the Western blots using an image analyzer (LAS 3000, Fujifilm, Japan). α-tubulin was used as a control. D . Representative MR images (n = 27) and photographs of Na + , K + -ATPase and AQP1 immunohistochemistry of the choroid epithelial cells of the control, CPA-treated and CGS21680-treated rats (n = 4). Scale bar, 100 μm. An A 1 agonist, CPA, and an A 2A agonist, CGS21680, were associated with ventriculomegaly. CPA but not CGS21680 was correlated with an increased expression of Na + , K + -ATPase but not AQP1. E . Representative Western blots of Na + , K + -ATPase and AQP1. F . Analysis of the Western blots using an image analyzer (LAS 3000, Fujifilm, Japan). α-tubulin was used as a control. Values are expressed as a percentage of the control (n = 6). The results were analyzed by one-way ANOVA followed by Tukey's multiple comparison. *, P
Figure Legend Snippet: The effect inversion of caffeine was associated with the induction of the A 1 adenosine receptor after long-term caffeine treatment . A . Representative photographs of adenosine A 1 and A 2A receptor immunohistochemistry in the choroid epithelial cells of the control and caffeine-treated rats (n = 4). Scale bar, 100 μm. B . Representative Western blots of the adenosine A 1 and A 2 receptors. C . Analysis of the Western blots using an image analyzer (LAS 3000, Fujifilm, Japan). α-tubulin was used as a control. D . Representative MR images (n = 27) and photographs of Na + , K + -ATPase and AQP1 immunohistochemistry of the choroid epithelial cells of the control, CPA-treated and CGS21680-treated rats (n = 4). Scale bar, 100 μm. An A 1 agonist, CPA, and an A 2A agonist, CGS21680, were associated with ventriculomegaly. CPA but not CGS21680 was correlated with an increased expression of Na + , K + -ATPase but not AQP1. E . Representative Western blots of Na + , K + -ATPase and AQP1. F . Analysis of the Western blots using an image analyzer (LAS 3000, Fujifilm, Japan). α-tubulin was used as a control. Values are expressed as a percentage of the control (n = 6). The results were analyzed by one-way ANOVA followed by Tukey's multiple comparison. *, P

Techniques Used: Immunohistochemistry, Western Blot, Expressing

Overproduction of CSF in caffeine-treated rats was associated with induction of Na + , K + -ATPase and increase in cerebral blood flow (CBF) . A . Representative photographs of Na + , K + -ATPase, AQP1 and carbonic anhydrase II (CAII) immunohistochemistry in the choroid epithelial cells of the control and caffeine-treated rats (n = 4). Scale bar, 100 μm. B . Representative Western blots of Na + , K + -ATPase, AQP1 and CAII. C . Analysis of Western blots by image analyzer (LAS 3000, Fujifilm, Japan). α-tubulin was used as a control. D . CBF was increased in the caffeine-treated rats. E . Cerebral perfusion was increased in the caffeine-treated rats. Values are expressed as the percentage of control (n = 10). *, P
Figure Legend Snippet: Overproduction of CSF in caffeine-treated rats was associated with induction of Na + , K + -ATPase and increase in cerebral blood flow (CBF) . A . Representative photographs of Na + , K + -ATPase, AQP1 and carbonic anhydrase II (CAII) immunohistochemistry in the choroid epithelial cells of the control and caffeine-treated rats (n = 4). Scale bar, 100 μm. B . Representative Western blots of Na + , K + -ATPase, AQP1 and CAII. C . Analysis of Western blots by image analyzer (LAS 3000, Fujifilm, Japan). α-tubulin was used as a control. D . CBF was increased in the caffeine-treated rats. E . Cerebral perfusion was increased in the caffeine-treated rats. Values are expressed as the percentage of control (n = 10). *, P

Techniques Used: Flow Cytometry, Immunohistochemistry, Western Blot

3) Product Images from "Alpha 2 Na+,K+-ATPase silencing induces loss of inflammatory response and ouabain protection in glial cells"

Article Title: Alpha 2 Na+,K+-ATPase silencing induces loss of inflammatory response and ouabain protection in glial cells

Journal: Scientific Reports

doi: 10.1038/s41598-017-05075-9

α2-Na + ,K + -ATPase is silenced by siRNA. ( A ) Representative western blotting of glial cells after 72 hours of treatment with Lipofectamine RNAiMAX of α2-Na + ,K + -ATPase siRNA and a scrambled sequence. ( B ) Western blotting bands were analyzed, and the α2/β-actin ratio was calculated (n = 6). *p
Figure Legend Snippet: α2-Na + ,K + -ATPase is silenced by siRNA. ( A ) Representative western blotting of glial cells after 72 hours of treatment with Lipofectamine RNAiMAX of α2-Na + ,K + -ATPase siRNA and a scrambled sequence. ( B ) Western blotting bands were analyzed, and the α2/β-actin ratio was calculated (n = 6). *p

Techniques Used: Western Blot, Sequencing

α2-Na + ,K + -ATPase is not related to changes in IL-10 levels. Cell culture supernatants of scrambled sequence and siRNA cells after treatment with LPS(1 µg/mL) and OUA (10 µM) were collected and used to measure IL-1β and IL-10 levels by ELISA (n = 6). ( A ) The levels of IL-1β in the scrambled group, *p
Figure Legend Snippet: α2-Na + ,K + -ATPase is not related to changes in IL-10 levels. Cell culture supernatants of scrambled sequence and siRNA cells after treatment with LPS(1 µg/mL) and OUA (10 µM) were collected and used to measure IL-1β and IL-10 levels by ELISA (n = 6). ( A ) The levels of IL-1β in the scrambled group, *p

Techniques Used: Cell Culture, Sequencing, Enzyme-linked Immunosorbent Assay

The lack of α2-Na + ,K + -ATPase decreases TNF-α levels. Cell culture supernatants of scrambled sequence and siRNA cells after treatment with LPS (1 µg/mL) and OUA (10 µM) were collected and used to measure TNF-α levels by ELISA (n = 6). ( A ) The levels of TNF-α in the scrambled group, *p
Figure Legend Snippet: The lack of α2-Na + ,K + -ATPase decreases TNF-α levels. Cell culture supernatants of scrambled sequence and siRNA cells after treatment with LPS (1 µg/mL) and OUA (10 µM) were collected and used to measure TNF-α levels by ELISA (n = 6). ( A ) The levels of TNF-α in the scrambled group, *p

Techniques Used: Cell Culture, Sequencing, Enzyme-linked Immunosorbent Assay

4) Product Images from "Vanadate-Induced Renal cAMP and Malondialdehyde Accumulation Suppresses Alpha 1 Sodium Potassium Adenosine Triphosphatase Protein Levels"

Article Title: Vanadate-Induced Renal cAMP and Malondialdehyde Accumulation Suppresses Alpha 1 Sodium Potassium Adenosine Triphosphatase Protein Levels

Journal: Toxicological Research

doi: 10.5487/TR.2018.34.2.143

Representative immunohistochemical staining images of renal α 1 -Na, K-ATPase protein localization in the cortex (A, D), outer medulla (B, E), and inner medulla (C, F) from sham (A–C), and vanadate (D–F) (n = 5/group). Vanadate reduced immunoreactivity both in the cortex (PCT and Pcap; arrows) and the medulla (PTs and MCD; arrow heads). Bar scale = 20 μm.
Figure Legend Snippet: Representative immunohistochemical staining images of renal α 1 -Na, K-ATPase protein localization in the cortex (A, D), outer medulla (B, E), and inner medulla (C, F) from sham (A–C), and vanadate (D–F) (n = 5/group). Vanadate reduced immunoreactivity both in the cortex (PCT and Pcap; arrows) and the medulla (PTs and MCD; arrow heads). Bar scale = 20 μm.

Techniques Used: Immunohistochemistry, Staining

5) Product Images from "Vanadate-Induced Renal cAMP and Malondialdehyde Accumulation Suppresses Alpha 1 Sodium Potassium Adenosine Triphosphatase Protein Levels"

Article Title: Vanadate-Induced Renal cAMP and Malondialdehyde Accumulation Suppresses Alpha 1 Sodium Potassium Adenosine Triphosphatase Protein Levels

Journal: Toxicological Research

doi: 10.5487/TR.2018.34.2.143

Effect of vanadate on renal α 1 -Na, K-ATPase protein abundance. Ten days of vanadate injection significantly suppressed α 1 -Na, K-ATPase protein abundance. Histogram bars show the densitometric analyses ratios of α 1 -Na, K-ATPase to β-actin intensity, and the representative immunoblot photographs are present. All values are expressed as the mean ± SD of 8 rats/group. *Significantly different from the sham group ( p
Figure Legend Snippet: Effect of vanadate on renal α 1 -Na, K-ATPase protein abundance. Ten days of vanadate injection significantly suppressed α 1 -Na, K-ATPase protein abundance. Histogram bars show the densitometric analyses ratios of α 1 -Na, K-ATPase to β-actin intensity, and the representative immunoblot photographs are present. All values are expressed as the mean ± SD of 8 rats/group. *Significantly different from the sham group ( p

Techniques Used: Injection

Related Articles

Blocking Assay:

Article Title: Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells
Article Snippet: .. Cells were blocked in immunocytochemical blocking buffer as described previously ( ) and were labeled with the following primary antibodies overnight at 4°C: rabbit anti-p75/NGFR (Cell Signaling Technology; Danvers, USA; Cat. No. 8238; 1:500 dilution), rabbit anti-SOX10 (Cell Signaling Technology; Cat. No. 89356; 1:500 dilution), mouse anti-zonula occludens-1 (Thermo Fisher Scientific; Cat. No. 33-9100; 1:500 dilution), rabbit anti-N-Cadherin (Cell Signaling Technology; Cat. No. 13116; 1:250 dilution), mouse anti-Aquaporin-1 (Abcam; Cat. No. ab9566; 1:250 dilution) or mouse anti-Na+ /K+ ATPase (Millipore; Cat. No. 05-369; 1:100 dilution). .. Sections of human cornea or differentiated CEnCs were labeled with rabbit anti-Cytokeratin 12 (Santa Cruz Biotechnology; Cat. No. SC-25722; 1:500 dilution) or mouse anti-Keratin 3/Keratin 76 (Millipore; Cat. No. CBL218-1; 1:500 dilution).

Purification:

Article Title: Na+/K+-ATPase ?1 Identified as an Abundant Protein in the Blood-Labyrinth Barrier That Plays an Essential Role in the Barrier Integrity
Article Snippet: .. Antibodies and purified proteins The following antibodies were used in this study: mouse monoclonal anti-actin (Cat# MAB1501, Chemicon), anti- Na+ /K+ -ATPase α1 (Cat# 05-369, Millipore), anti-desmin (Cat# ab6322, Abcam), anti-PKCη (Cat# 610814, BD Transduction); rabbit polyclonal anti-Kir 4.1(Cat# AB5818, Chemicon), anti-occludin (Cat# 40-4700, Invitrogen), anti-ZO-1 (Cat# 40-2300, Invitrogen), anti-Collagen type IV (Cat# ab6586, Abcam), anti-NG2 (Cat# NBP1-20170, Novus Biologicals), anti-PECAM-1(Cat# ab24590, Abcam). .. The purified recombinant proteins used in this study were: ATP1A1 (Cat# TP301009) from Origene Technologies, Inc. and PKCη (Cat# 10-782-55103) from Geneway Biotech, Inc.

Labeling:

Article Title: Feeder-free differentiation of cells exhibiting characteristics of corneal endothelium from human induced pluripotent stem cells
Article Snippet: .. Cells were blocked in immunocytochemical blocking buffer as described previously ( ) and were labeled with the following primary antibodies overnight at 4°C: rabbit anti-p75/NGFR (Cell Signaling Technology; Danvers, USA; Cat. No. 8238; 1:500 dilution), rabbit anti-SOX10 (Cell Signaling Technology; Cat. No. 89356; 1:500 dilution), mouse anti-zonula occludens-1 (Thermo Fisher Scientific; Cat. No. 33-9100; 1:500 dilution), rabbit anti-N-Cadherin (Cell Signaling Technology; Cat. No. 13116; 1:250 dilution), mouse anti-Aquaporin-1 (Abcam; Cat. No. ab9566; 1:250 dilution) or mouse anti-Na+ /K+ ATPase (Millipore; Cat. No. 05-369; 1:100 dilution). .. Sections of human cornea or differentiated CEnCs were labeled with rabbit anti-Cytokeratin 12 (Santa Cruz Biotechnology; Cat. No. SC-25722; 1:500 dilution) or mouse anti-Keratin 3/Keratin 76 (Millipore; Cat. No. CBL218-1; 1:500 dilution).

other:

Article Title: G protein-coupled receptor 37L1 regulates renal sodium transport and blood pressure
Article Snippet: GPR37L1, Na+ /H+ exchanger isoform 3 (NHE3), Na+ -K+ -ATPase (NKA), and sodium-glucose cotransporter 2 (SGLT2) proteins were measured by a standard protocol using a validated goat polyclonal anti-GPR37L1 antibody (sc-164532; Santa Cruz Biotechnology), chicken polyclonal antibody against rodent NHE3, mouse monoclonal antibody against NKA (05-369; MilliporeSigma, Burlington, MA), or rabbit polyclonal antibody against SGLT2 (sc-98975; Santa Cruz Biotechnology), respectively.

Immunostaining:

Article Title: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth
Article Snippet: .. For immunostaining antibodies used - anti-α1 Na/K-ATPase antibody (Millipore Cat# 05-369) and Alexa Fluor 488-conjugated anti-mouse secondary antibody. .. Cell growth assay and MTT assay Cell growth assay and MTT assay were performed as previously described , .

Western Blot:

Article Title: Role of the Na+/H+ exchanger 3 in angiotensin II-induced hypertension in NHE3-deficient mice with transgenic rescue of NHE3 in small intestines
Article Snippet: .. For western blot analysis, the following antibodies were used: rabbit polyclonal anti-Na+ /HCO3 cotransporter (NBC) targeting the N-terminus 338–391 of the rat kidney Na+ /HCO3 cotransporter (Abcam, Cambridge, MA, Cat. #AB3212), the mouse monoclonal anti-Na+ /K+ -ATPase recognizing the α 1 subunit isoform of Na+ /K+ -ATPase (Millipore Cat., Billerica, MA, #05-369; Lot: #DAM1794271), the rabbit polyclonal antiprotein kinase Cα antibody targeting phospho-S657+Y658 (Abcam Cat. #ab23513), antiglycogen synthase kinase 3 α /β antibody (GSKα /β ) recognizing phospho-Y216+Y279 (Abcam Cat. #ab4797), the goat polyclonal antiaquaporin 1 (AQP1) antibody targeting the C-terminus of AQP1 of human origin (Santa Cruz Cat., Dallas, TX, #sc-9878), and the mouse monoclonal anti-MAP kinases ERK1/2 antibody recognizing phospho-Tyr 204 (Santa Cruz Cat. #sc-7383), respectively. ..

Article Title: Tamoxifen Decreases Lithium-Induced Natriuresis in Rats With Nephrogenic Diabetes Insipidus
Article Snippet: .. For western blot, the following antibodies were used: αENaC, βENaC, γENaC , NHE3 (ab95299, Abcam, Cambridge, United Kingdom), NaPi (LL696AP) ( ; ), NKCC2 (LL320AP) ( ; ; ), and Na/K-ATPase (Millipore, 05-369). .. The following antibodies were used for IHC: βENaC (GTX41971, GeneTeX, Irvine, CA, United States) and γENaC ( ).

SDS Page:

Article Title: Identification and Characterization of Mechanism of Action of P61-E7, a Novel Phosphine Catalysis-Based Inhibitor of Geranylgeranyltransferase-I
Article Snippet: .. Proteins were then resolved by 12% or 12.5% SDS-PAGE and immunoblotted with antibodies against unprenylated form of Rap1 (U-Rap1; Santa Cruz Biotechnology, Inc.), p21CIP1/WAF1 (Millipore, Temecula, CA), p27Kip1 (rabbit, Santa Cruz Biotechnology, Inc.), phospho-p27Kip1 (T187) (Invitrogen, Carlsbad, CA), HDJ-2 (NeoMarkers, Fremont, CA), RhoGDI (Santa Cruz Biotechnology, Inc.), Na+ /K+ ATPase-α (Sigma), RalA (BD Bioscience), HA.11 (Covance) and Actin (Calbiochem). .. Detection was performed using peroxidase-conjugated secondary antibodies (Biorad) and Amersham ECL Plus™ Western Blotting Detection Reagents (GE Healthcare Life Sciences).

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  • 88
    Millipore na k atpase activity
    PKCη phosphorylated occludin from decreased Na + /K + <t>-ATPase</t> activity is a mechanism for noise-induced blood-labyrinth barrier disruption. A, Confocal images of IgG leakage from capillaries in disrupted blood-labyrinth barrier of noise-exposed (NE) animals. Stria vascularis capillary networks were labeled with anti-collagen type IV (red) and anti-serum protein IgG (H+L, green) antibodies. IgG was confined in normal stria vascularis capillaries (Left) but not in NE animals (Right). B, REE analysis shows significantly increased blood-labyrinth barrier permeability in NE mice (**P = 0.005
    Na K Atpase Activity, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na k atpase activity/product/Millipore
    Average 88 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    na k atpase activity - by Bioz Stars, 2020-05
    88/100 stars
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    89
    Millipore na k atpase α1
    Expressions of NCX1 ( A ) and the major subunits of Na + /K + <t>-ATPase</t> ( B ) in adult myocytes isolated from Wt and NCX1 KO hearts. Myocyte isolation and protein immunoassays were done as indicated in methods . GAPDH was used as a loading control. n = 3–4,
    Na K Atpase α1, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na k atpase α1/product/Millipore
    Average 89 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    na k atpase α1 - by Bioz Stars, 2020-05
    89/100 stars
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    94
    Millipore na k atpase
    TRPM2 channels are expressed in the sarcolemma and transverse tubules of adult cardiac myocytes. Confocal images of adult WT mouse ventricular myocytes labeled with anti-α 1 -subunit of Na + -K + <t>-ATPase</t> antibody ( A and D ), with ( B ) or without ( E ) rabbit
    Na K Atpase, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na k atpase/product/Millipore
    Average 94 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    na k atpase - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    PKCη phosphorylated occludin from decreased Na + /K + -ATPase activity is a mechanism for noise-induced blood-labyrinth barrier disruption. A, Confocal images of IgG leakage from capillaries in disrupted blood-labyrinth barrier of noise-exposed (NE) animals. Stria vascularis capillary networks were labeled with anti-collagen type IV (red) and anti-serum protein IgG (H+L, green) antibodies. IgG was confined in normal stria vascularis capillaries (Left) but not in NE animals (Right). B, REE analysis shows significantly increased blood-labyrinth barrier permeability in NE mice (**P = 0.005

    Journal: PLoS ONE

    Article Title: Na+/K+-ATPase ?1 Identified as an Abundant Protein in the Blood-Labyrinth Barrier That Plays an Essential Role in the Barrier Integrity

    doi: 10.1371/journal.pone.0016547

    Figure Lengend Snippet: PKCη phosphorylated occludin from decreased Na + /K + -ATPase activity is a mechanism for noise-induced blood-labyrinth barrier disruption. A, Confocal images of IgG leakage from capillaries in disrupted blood-labyrinth barrier of noise-exposed (NE) animals. Stria vascularis capillary networks were labeled with anti-collagen type IV (red) and anti-serum protein IgG (H+L, green) antibodies. IgG was confined in normal stria vascularis capillaries (Left) but not in NE animals (Right). B, REE analysis shows significantly increased blood-labyrinth barrier permeability in NE mice (**P = 0.005

    Article Snippet: Enzymatic activity assay Both PKCη and Na+ /K+ -ATPase activity were respectively determined with a PKCη KinEASETM FP Fluorescein Green Assay Kit (Millipore) and an ATPase assay kit (Novus Biologicals).

    Techniques: Activity Assay, Labeling, Permeability, Mouse Assay

    Decreased Na + /K + -ATPase activity increases TJ permeability via PKCη phosphorylated occludin. A and B , Co-immunoprecipitation using isolated stria vascularis capillary lysates shows that PKCη is in a complex with ATP1A1. Goat IgG served as a negative control. C , Protein-protein interaction analysis with purified ATP1A1 (250 ng) and PKCη (250 ng). Ouabain (10 µM) was added where indicated. Control lanes consisted of either anti-ATP1A1 antibody and purified PKCη or anti-PKCη antibody and purified ATP1A1. D , Ouabain inhibition of Na + /K + -ATPase activity causes increased PKCη activity in isolated stria vascularis capillaries (*P = 0.013

    Journal: PLoS ONE

    Article Title: Na+/K+-ATPase ?1 Identified as an Abundant Protein in the Blood-Labyrinth Barrier That Plays an Essential Role in the Barrier Integrity

    doi: 10.1371/journal.pone.0016547

    Figure Lengend Snippet: Decreased Na + /K + -ATPase activity increases TJ permeability via PKCη phosphorylated occludin. A and B , Co-immunoprecipitation using isolated stria vascularis capillary lysates shows that PKCη is in a complex with ATP1A1. Goat IgG served as a negative control. C , Protein-protein interaction analysis with purified ATP1A1 (250 ng) and PKCη (250 ng). Ouabain (10 µM) was added where indicated. Control lanes consisted of either anti-ATP1A1 antibody and purified PKCη or anti-PKCη antibody and purified ATP1A1. D , Ouabain inhibition of Na + /K + -ATPase activity causes increased PKCη activity in isolated stria vascularis capillaries (*P = 0.013

    Article Snippet: Enzymatic activity assay Both PKCη and Na+ /K+ -ATPase activity were respectively determined with a PKCη KinEASETM FP Fluorescein Green Assay Kit (Millipore) and an ATPase assay kit (Novus Biologicals).

    Techniques: Activity Assay, Permeability, Immunoprecipitation, Isolation, Negative Control, Purification, Inhibition

    Expressions of NCX1 ( A ) and the major subunits of Na + /K + -ATPase ( B ) in adult myocytes isolated from Wt and NCX1 KO hearts. Myocyte isolation and protein immunoassays were done as indicated in methods . GAPDH was used as a loading control. n = 3–4,

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Different roles of the cardiac Na+/Ca2+-exchanger in ouabain-induced inotropy, cell signaling, and hypertrophy

    doi: 10.1152/ajpheart.00462.2012

    Figure Lengend Snippet: Expressions of NCX1 ( A ) and the major subunits of Na + /K + -ATPase ( B ) in adult myocytes isolated from Wt and NCX1 KO hearts. Myocyte isolation and protein immunoassays were done as indicated in methods . GAPDH was used as a loading control. n = 3–4,

    Article Snippet: Primary antibodies and their sources were as follows: RDI Research Diagnostics, rabbit anti-Na+ /Ca2+ exchanger; BD Transduction Laboratories, anti-PI3K p85; Cell Signaling Technology, rabbit anti-phospho 473-Akt and anti-Akt; Developmental Studies Hybridoma Bank, University of Iowa: Na+ -K+ -ATPase α1 (α6F); ABR, Na+ /K+ -ATPase α2 ; Millipore, Na+ /K+ ATPase β1; Santa Cruz, anti-phospho-ERK and ERK.

    Techniques: Isolation

    Schematic presentation of the two parallel cell signaling cascades that are linked to digitalis-inhibited Na + /K + -ATPase of the cardiac myocytes, and their relations to digitalis-induced hypertrophy and positive inotropy. See discussion .

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Different roles of the cardiac Na+/Ca2+-exchanger in ouabain-induced inotropy, cell signaling, and hypertrophy

    doi: 10.1152/ajpheart.00462.2012

    Figure Lengend Snippet: Schematic presentation of the two parallel cell signaling cascades that are linked to digitalis-inhibited Na + /K + -ATPase of the cardiac myocytes, and their relations to digitalis-induced hypertrophy and positive inotropy. See discussion .

    Article Snippet: Primary antibodies and their sources were as follows: RDI Research Diagnostics, rabbit anti-Na+ /Ca2+ exchanger; BD Transduction Laboratories, anti-PI3K p85; Cell Signaling Technology, rabbit anti-phospho 473-Akt and anti-Akt; Developmental Studies Hybridoma Bank, University of Iowa: Na+ -K+ -ATPase α1 (α6F); ABR, Na+ /K+ -ATPase α2 ; Millipore, Na+ /K+ ATPase β1; Santa Cruz, anti-phospho-ERK and ERK.

    Techniques:

    TRPM2 channels are expressed in the sarcolemma and transverse tubules of adult cardiac myocytes. Confocal images of adult WT mouse ventricular myocytes labeled with anti-α 1 -subunit of Na + -K + -ATPase antibody ( A and D ), with ( B ) or without ( E ) rabbit

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: The second member of transient receptor potential-melastatin channel family protects hearts from ischemia-reperfusion injury

    doi: 10.1152/ajpheart.00906.2012

    Figure Lengend Snippet: TRPM2 channels are expressed in the sarcolemma and transverse tubules of adult cardiac myocytes. Confocal images of adult WT mouse ventricular myocytes labeled with anti-α 1 -subunit of Na + -K + -ATPase antibody ( A and D ), with ( B ) or without ( E ) rabbit

    Article Snippet: Immunolocalization experiments indicated that both TRPM2 channels and the α1 -subunit of Na+ -K+ -ATPase (sarcolemma marker) were expressed in the sarcolemma and transverse tubules in adult LV myocytes ( ).

    Techniques: Labeling

    I/R decreases Na + -K + -ATPase current ( I pump ) more in TRPM2 KO myocytes. WT or KO hearts were subjected to either sham operation or 30 min ischemia of followed by 3 days of reperfusion before myocyte isolation. LV myocytes were held at 0 mV and 30°C.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: The second member of transient receptor potential-melastatin channel family protects hearts from ischemia-reperfusion injury

    doi: 10.1152/ajpheart.00906.2012

    Figure Lengend Snippet: I/R decreases Na + -K + -ATPase current ( I pump ) more in TRPM2 KO myocytes. WT or KO hearts were subjected to either sham operation or 30 min ischemia of followed by 3 days of reperfusion before myocyte isolation. LV myocytes were held at 0 mV and 30°C.

    Article Snippet: Immunolocalization experiments indicated that both TRPM2 channels and the α1 -subunit of Na+ -K+ -ATPase (sarcolemma marker) were expressed in the sarcolemma and transverse tubules in adult LV myocytes ( ).

    Techniques: Isolation